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See detailProtective effect of a new CHITOSAN BIOMATERIAL against the development of experimental osteoarthritis lesions in rabbit
Oprenyeszk, Frédéric ULg; Chausson, M; Maquet, V et al

Conference (2013, November 23)

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See detailProtective effect of a new biomaterial against the development of experimental osteoarthritis lesions in rabbit: a pilot study evaluating the intra-articular injection of alginate-chitosan beads dispersed in an hydrogel.
Oprenyeszk, Frédéric ULg; Chausson, Mickael; Maquet, Véronique et al

in Osteoarthritis and Cartilage (2013), 21(8), 1099-1107

Objective: This study aimed to evaluate the structural benefit of a new biomaterial composed of alginate-chitosan (AC) beads dispersed in an hydrogel (H) derived from chitosan on the development of ... [more ▼]

Objective: This study aimed to evaluate the structural benefit of a new biomaterial composed of alginate-chitosan (AC) beads dispersed in an hydrogel (H) derived from chitosan on the development of osteoarthritis (OA) in rabbit. Design: OA was induced by the surgical transection of the anterior cruciate ligament in rabbits. Animals received a single intra-articular injection (900 μl) of AC beads in H hydrogel, H hydrogel alone or saline one week after surgery. OA development was followed by X-rays. Blood samples were collected throughout the study to measure biological markers (PGE2 and CRP). Macroscopic observation and histological evaluation of articular cartilage and synovial membrane were performed 6 weeks after surgery. Results: AC beads in H hydrogel prevented from the development of OA based on the reduction of the Kellgren & Lawrence (K&L) score. It also significantly reduced the histological score of cartilage lesion severity. This effect was homogenous on every joint compartment. It was due to a significant effect on cartilage structure and cellularity scores. The injection of AC beads in H hydrogel also tended to reduce the synovial membrane inflammation. No significant variation of biological markers was noted. Conclusions: The present pilot study provides interesting and promising results for the use of AC beads in H hydrogel in animal. It indeed prevented the development of OA cartilage lesions without inflammatory signs. The potencies of this biomaterial to protect OA joint should be further documented. It could then represent a new alternative for viscosupplementation in human OA management. [less ▲]

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See detailProtective effect of EGB 761 against oxidative phosphorylation damages of brain mitochondria after Anoxia/reoxygenation in vivo and in vitro
Du, G.; Willet, K.; Jarmuszkiewicz, W. et al

in Toxicology Mechanisms & Methods (2004), 14

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See detailProtective effect of Ginkgo biloba extract (Egb 761) on functional impairments of mitochondria induced by anoxia-reoxygenation in situ and in vitro
Sluse, Francis ULg; DU, G.-H.; Willet, K. et al

in Packer, L.; Christen, Y. (Eds.) Gingko biloba extract (EGb 761) : lessons from cell biology (1998)

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See detailProtective effect of prion protein via the N-terminal region in mediating a protective effect on paraquat-induced oxidative injury in neuronal cells.
Dupiereux-Fettweis, Ingrid ULg; Falisse-Poirier, Nandini; Zorzi, Willy ULg et al

in Journal of Neuroscience Research (2008), 86(3), 653-9

Transmissible spongiform encephalopathies are a group of neurodegenerative disorders caused by a posttranslational, conformational change in the cellular isoform of the prion protein (PrP(C)) into an ... [more ▼]

Transmissible spongiform encephalopathies are a group of neurodegenerative disorders caused by a posttranslational, conformational change in the cellular isoform of the prion protein (PrP(C)) into an infectious, disease-associated form (PrP(Sc)). Increasing evidence supports a role for PrP(C) in the cellular response to oxidative stress. We investigated the effect of oxidative stress mediated by paraquat exposure on SH-SY5Y neuroblastoma cells. A loss of mitochondrial membrane potential and subsequent reduction in ATP production were demonstrated in untransfected SH-SY5Y cells, an effect that was ameliorated by the expression of PrP(C). Cells expressing either PrP-DeltaOct, which lacks the octapeptide repeats, or PrP-DA, in which the N-terminus is tethered to the membrane, showed increased sensitivity to paraquat compared with cells expressing wild-type PrP(C) as shown by reduced viability, loss of their membrane integrity, and reduced mitochondrial bioenergetic measurements. Exposure of prion-infected mouse SMB15S cells to paraquat resulted in a reduction in viability to levels similar to those seen in the untransfected SH-SY5Y cells. However, "curing" the cells with pentosan sulfate restored the viability to the level observed in the SH-SY5Y cells expressing PrP(C). These data would indicate that the molecular mechanism promoting cellular resistance to oxidative stress had been compromised in the infected SMB15S cells, which could be reinstated upon curing. Our study supports the hypothesis that PrP(C) expression protects cells against paraquat-induced oxidative injury, demonstrates the significance of the N-terminal region of the protein in mediating this protective effect, and also shows that the biochemical consequences of prion infection may be reversed with therapeutic intervention. [less ▲]

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See detailProtective Effect of Various Antagonists of Inflammatory Mediators against Paraoxon-Induced Pulmonary Edema in the Rabbit
Delaunois, Annie ULg; Gustin, Pascal ULg; Vargas, M. et al

in Toxicology and Applied Pharmacology (1995), 132(2), 343-345

The protective effect of some antagonists of various inflammatory mediators against paraoxon-induced increases in endothelial permeability has been investigated in isolated perfused rabbit lungs. The ... [more ▼]

The protective effect of some antagonists of various inflammatory mediators against paraoxon-induced increases in endothelial permeability has been investigated in isolated perfused rabbit lungs. The edema induced by paraoxon has been previously related to a chain reaction mediated by acetylcholine. Lungs were ventilated and blood-free perfused with a constant flow. Arterial and venous pressures and lung weight were continuously recorded. Endothelial permeability was evaluated by measuring the capillary filtration coefficient (Kf,c). Paraoxon (4 x 10(-4) M) was injected in the perfusion circuit, in lungs with or without pretreatment with atropine, ketanserin, clonidine, morphine, indomethacin, and terfenadine plus cimetidine. Paraoxon induced a time-dependent increase in the Kf,c, a maximal effect being recorded 60 min after the injection. All the antagonists used as pretreatment significantly reduced the maximal effect recorded after paraoxon. These results show that muscarinic receptor antagonists, inhibitors of neuropeptides release, cyclooxygenase inhibitors, and 5-hydroxytryptamine and histamine receptor antagonists can protect the lung against the edema induced by paraoxon. This protective effect is due to inhibition of the chain reaction triggered by acetylcholine. [less ▲]

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See detailProtective Effects Of A Live Attenuated Bovine Leukaemia Virus Vaccine With Deletion In The R3 And G4 Genes
Reichert, M.; Cantor, Gh.; Willems, Luc ULg et al

in Journal of General Virology (2000), 81

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See detailProtective Effects of Web 2086 (Paf Antagonist) and Ketoprofen (Nsaid) on Paf-Induced Changes in the Morphological Ultrastructure of Blood Platelets in Calves
Bastos da Silva, Miriam; Doizé, Cécile ULg; Coghe, Joost et al

in Veterinary Research Communications (1998), 22(4), 273-291

The ultrastructure of bovine platelets was examined by transmission electron microscopy without any pretreatment (control), and after WEB 2086 (a triazolodiazepine) or ketoprofen (NSAID) pretreatment ... [more ▼]

The ultrastructure of bovine platelets was examined by transmission electron microscopy without any pretreatment (control), and after WEB 2086 (a triazolodiazepine) or ketoprofen (NSAID) pretreatment, followed by PAF infusion. The blood platelet count was also investigated. The group of calves that received WEB 2086 pretreatment before platelet-activating factor (PAF) infusion did not show a decreased number of platelets. However, in the other group, with ketoprofen pretreatment before PAF infusion, there was a rapid decrease from 1 to 3 min, while from 5 min the number of platelets recovered to the normal value. Electron microscopy revealed that pretreatment with WEB 2086 followed by PAF infusion did not alter the morphological ultrastructure of bovine platelets, except that the microtubules were briefly modified from 1 until 3 min after PAF challenge. After ketoprofen pretreatment, bovine platelets kept their regular shape, the number of dense bodies was not significantly altered, the number of mitochondria was maintained from 5 min after PAF infusion, giant platelets were not observed and the Golgi apparatus was rarely visible. Thus pretreatment with WEB 2086 and ketoprofen before PAF infusion had a protective activity on the ultrastructure of bovine platelets and, in cattle, pretreatment with WEB 2086 and ketoprofen before PAF challenge prevented the thrombocytopenia induced by PAF. [less ▲]

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See detailProtective role of P2Y(2) receptor against lung infection induced by pneumonia virus of mice
Vanderstocken, Gilles; Van de Paar, Els; Robaye, Benoit et al

in PLoS ONE (2012), 7(11), 50385

ATP released in the early inflammatory processes acts as a danger signal by binding to purinergic receptors expressed on immune cells. A major contribution of the P2Y2 receptor of ATP/UTP to dendritic ... [more ▼]

ATP released in the early inflammatory processes acts as a danger signal by binding to purinergic receptors expressed on immune cells. A major contribution of the P2Y2 receptor of ATP/UTP to dendritic cell function and Th2 lymphocyte recruitment during asthmatic airway inflammation was previously reported. We investigated here the involvement of P2Y2 receptor in lung inflammation initiated by pneumonia virus of mice infection. We demonstrated that P2Y2-/- mice display a severe increase in morbidity and mortality rate in response to the virus. Lower survival of P2Y2-/- mice was not correlated with excessive inflammation despite the higher level of neutrophil recruiters in their bronchoalveolar fluids. Interestingly, we observed reduced ATP level and lower numbers of dendritic cells, CD4+ T cells and CD8+ T cells in P2Y2-/- compared to P2Y2+/+ infected lungs. Lower level of IL-12 and higher level of IL-6 in bronchoalveolar fluid support an inhibition of Th1 response in P2Y2-/- infected mice. Quantification of DC recruiter expression revealed comparable IP-10 and MIP-3 levels but a reduced BRAK level in P2Y2-/- compared to P2Y2+/+ bronchoalveolar fluids. Higher morbidity and mortality of P2Y2-/- mice appear to result from defective dendritic cell and T cell infiltration that were correlated with higher virus titer. In conclusion, P2Y2 receptor previously described as a target in cystic fibrosis therapy and as a mediator of Th2 response in asthma, may also regulate Th1 response protecting mice against lung viral infection. [less ▲]

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See detailLa « Protectrice du Païs-Bas ». Stratégies politiques et figures de la Vierge dans les Pays-Bas espagnols
Delfosse, Annick ULg

Book published by Brepols Publishers (2009)

Dans les Pays-Bas espagnols, morcelés par les revendications particularistes provinciales et bouleversés par d'incessantes guerres, se construit tout au long du XVIIe siècle une figure originale de la ... [more ▼]

Dans les Pays-Bas espagnols, morcelés par les revendications particularistes provinciales et bouleversés par d'incessantes guerres, se construit tout au long du XVIIe siècle une figure originale de la Vierge. "Reine de Guerre" et "Victorieuse", garante du pouvoir des Habsbourg et Patronne des villes, elle entre dans l'appareil symbolique que forgent les autorités pour promettre aux populations l'ordre espéré et fonder par la même occasion leur légitimité. L'A. repère et analyse les procédés qui ont contribué à définir ce rôle politique d'une Vierge puissante, triomphale et souveraine. Elle montre comment émerge cette fonction mariale tant dans la littérature pèlerine, dévotionnelle et théologique que dans les pratiques des communautés urbaines et de la Cour installée à Bruxelles [less ▲]

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See detailProtégeons la beauté du ciel nocturne-I
Jehin, Emmanuel ULg; Demoulin, Philippe ULg

Article for general public (2000)

Not Available

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See detailProtégeons la beauté du ciel nocturne-II
Jehin, Emmanuel ULg; Demoulin, Philippe ULg

Article for general public (2000)

Not Available

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See detailProtégeons nos mammifères: le point cinq ans après
Libois, Roland ULg

Learning material (1987)

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See detailProtein adsorption on preadsorbed polyampholytic monolayers
Mahltig, Boris; Werner, Carsten; Müller, Martin et al

in Journal of Biomaterials Science. Polymer Edition (2001), 12(9), 995-1010

The adsorption behaviour of five different globular proteins on pure silicon substrates and on preadsorbed polyampholytic monolayers has been investigated as a function of protein concentration. The ... [more ▼]

The adsorption behaviour of five different globular proteins on pure silicon substrates and on preadsorbed polyampholytic monolayers has been investigated as a function of protein concentration. The prelayers were prepared by adsorption of the ampholytic diblock copolymer poly(methacrylic acid)-block-poly ((dimethylamino)ethyl methacrylate) (PMAA-b-PDMAEMA). This polyampholyte adsorbs in densely packed micelles directly from aqueous solution. Ellipsometry was used to determine the amount of adsorbed polyampholyte and protein. While ATR-IR spectroscopy gives information about the adsorption and desorption behaviour of the preadsorbed polyampholytic layer, the lateral structures of the dried films were investigated by scanning force microscopy (SFM). The amount of protein adsorbed was found to be strongly influenced by the preadsorbed polyampholyte compared to the adsorption on the pure silicon substrates. No displacement of the polyampholyte by the proteins was detected. In most cases the protein adsorption was reduced by the preadsorbed polyampholytic layer. The observed trends are explained by the change in electrostatic and hydrophilic characteristics of the substrates. Furthermore, the entropy of adsorption has to be taken into account. [less ▲]

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See detailProtein And Amino Acid Profiles Of Tunisian Deglet Nour And Allig Date Palm Fruit Seeds
Bouaziz, Mohamed; Besbes, Souhail; Blecker, Christophe ULg et al

in Fruits (2008), 63(1),

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See detailProtein and crude fiber determination on ground or whole seeds triticale by near infrared reflectance or transmittance spectroscopy.
Leterme, Pascal; Dardenne, Pierre; Thewis, André ULg et al

in Proceedings of the Second International Tritical Symposium, Passo Fundo (Brazil) (1990)

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See detailProtein and crude fiber determination on tritical: NI and NIT comparison.
Leterme, Pascal; Dardenne, Pierre; Thewis, André ULg et al

in Biston, Robert; Barthiaux-Thill, Nicole (Eds.) Proceedings of the Third International Conference on Near Infrared Spectroscopy, vol.2 (1990)

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See detailProtein composition and agglomeration tendency of gluten isolated from European wheats (Triticum aestivum L.) in a batter system
Roels, S. P.; Sindic, Marianne ULg; Deroanne, Claude et al

in Journal of Agricultural and Food Chemistry (1998), 46(4), 1344-1349

Pilot scale isolation of gluten (with recovery of gluten on 400, 250, and 125 mu sieves) from flour prepared from six European wheat varieties (Apollo, Slejpner, Sperber, Camp Remy, Minaret, and Soissons ... [more ▼]

Pilot scale isolation of gluten (with recovery of gluten on 400, 250, and 125 mu sieves) from flour prepared from six European wheat varieties (Apollo, Slejpner, Sperber, Camp Remy, Minaret, and Soissons) resulted in, on average, gluten yields of 9.6% (4.7-13.2% range). Gluten protein recoveries averaged 63.0% (34.5-85.7% range). Gluten yields and gluten protein recoveries were linearly related and increased when mixing times and baking absorptions required for optimal dough development of the parent flours increased, indicating that there is a relationship between the agglomeration properties of gluten proteins in a batter system and the optimal technological conditions necessary for processing the flours in breadmaking. The Osborne protein fractions in the gluten fractions were determined. The 0.05 M acetic acid soluble (glutenin) fraction was quantitatively the most important fraction (40-46% of gluten Kjeldahl nitrogen) while comparable levels of 70% ethanol soluble (gliadins) and 0.05 M acetic acid insoluble (residue protein) were found (17.9-22.3% of gluten Kjeldahl nitrogen and 22.0-29.0% of gluten Kjeldahl nitrogen for gliadins and residue protein, respectively). With decreasing pore size of the sieves, the level of glutenin in the gluten decreased while the level of gliadins increased. This indicates that, in gluten with good agglomeration properties, the level of glutenins is high and that the agglomeration properties of such proteins (i.e. their tendency to aggregate) strongly determines the agglomeration behavior of the gluten as a whole. [less ▲]

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