Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailQuantification of Bifidobacterium ssp and Lactobacillus spp in rat fecal samples by real-time PCR
Delroisse, Jean-Marc; Boulvin, Anne-Lise; Parmentier, Isabelle et al

in Archives Internationales de Physiologie et de Biochimie (2005), 191

Detailed reference viewed: 26 (3 ULg)
Peer Reviewed
See detailQuantification of bST in milk by electrochemiluminescence: a way for detection of treated cows.
Renaville, Robert ULg; Deaver, D.; Baronheid, Christophe et al

in Proceedings of the 4th International Conference on Farm Animal Endocrinology (2001, October)

Detailed reference viewed: 17 (0 ULg)
Peer Reviewed
See detailQuantification of cardiac IGF-1 expression by the real-time taqman PCR : development and first application in a canine model of congestive heart failure
Motte, S.; Van Beneden, R.; Mottet, J. et al

in 11th ESVIM Meeting - Dublin - Irlande - 2001 (2001)

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailQuantification of dandruff adherence to hair.
Pierard, Claudine ULg; Uhoda, Emmanuelle ULg; Pierard, Gérald ULg

in International Journal of Cosmetic Science (2005), 27(5), 279-82

Dandruff adherence to hair shafts is a visible annoying phenomenon. The aspect can be recorded by different means including the ultraviolet light-enhanced visualization (ULEV) method and microscopy. We ... [more ▼]

Dandruff adherence to hair shafts is a visible annoying phenomenon. The aspect can be recorded by different means including the ultraviolet light-enhanced visualization (ULEV) method and microscopy. We present another quantitative method. Hairs were clipped from the parietal scalp area of 25 volunteers complaining of dandruff. They were firmly applied onto cyanoacrylate-coated microscopic slides. Hairs were lifted up after 15-20 s leaving a cast in the adhesive coat. Dandruff were thus harvested from the hair shafts and remained attached to the cyanoacrylate coat. After staining, the material was submitted to image analysis to derive the dandruff density per unit length of hairs. In 11/25 subjects, a correlation was found between the width of the hair casts and the dandruff density. This method does not collect all dandruff along hair shafts, but data are likely representative of the whole corneocyte load. [less ▲]

Detailed reference viewed: 18 (0 ULg)
See detailQuantification of drugs in plasma of injured drivers
Charlier, Corinne ULg

Conference (1997)

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailQuantification of Equid herpesvirus 5 DNA in clinical and necropsy specimens collected from a horse with equine multinodular pulmonary fibrosis
Marenzoni, M. L.; Passamonti, F.; Lepri, E. et al

in Journal of Veterinary Diagnostic Investigation (2011), 23

Detailed reference viewed: 9 (3 ULg)
Full Text
Peer Reviewed
See detailQuantification of fetal head direction and descent.
Tutschek, B.; Braun, T.; CHANTRAINE, Frédéric ULg et al

in Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology (2013), 41(1), 99-100

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailQuantification of HTLV-1 Clonality and TCR Diversity.
Laydon, Daniel J.; Melamed, Anat; Sim, Aaron et al

in PLoS computational biology (2014), 10(6), 1003646

Estimation of immunological and microbiological diversity is vital to our understanding of infection and the immune response. For instance, what is the diversity of the T cell repertoire? These questions ... [more ▼]

Estimation of immunological and microbiological diversity is vital to our understanding of infection and the immune response. For instance, what is the diversity of the T cell repertoire? These questions are partially addressed by high-throughput sequencing techniques that enable identification of immunological and microbiological "species" in a sample. Estimators of the number of unseen species are needed to estimate population diversity from sample diversity. Here we test five widely used non-parametric estimators, and develop and validate a novel method, DivE, to estimate species richness and distribution. We used three independent datasets: (i) viral populations from subjects infected with human T-lymphotropic virus type 1; (ii) T cell antigen receptor clonotype repertoires; and (iii) microbial data from infant faecal samples. When applied to datasets with rarefaction curves that did not plateau, existing estimators systematically increased with sample size. In contrast, DivE consistently and accurately estimated diversity for all datasets. We identify conditions that limit the application of DivE. We also show that DivE can be used to accurately estimate the underlying population frequency distribution. We have developed a novel method that is significantly more accurate than commonly used biodiversity estimators in microbiological and immunological populations. [less ▲]

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailQuantification of in Vivo Tumor Invasion and Vascularization by Computerized Image Analysis
Blacher, Silvia ULg; Jost, M.; Melen-Lamalle, Laurence ULg et al

in Microvascular Research (2008), 75(2), 169-78

The matrix-inserted surface transplantation model is an in vivo assay used to analyse the kinetics of tumor-vessel interactions during different stages of skin carcinoma progression. This system allows ... [more ▼]

The matrix-inserted surface transplantation model is an in vivo assay used to analyse the kinetics of tumor-vessel interactions during different stages of skin carcinoma progression. This system allows the study of host-tumor interface, i.e. penetration of tumor cells into normal host tissue as well as infiltration of normal host cells into the tumor. In the present study, image analysis algorithms for processing and quantifying the extent of such migratory and tissue remodeling events are presented. The proposed method is non-parametric and its originality lies in its particularity to take into account the specific geometry of tumor-host interface. This methodology is validated by evaluating the contribution of matrix metalloproteases (MMPs) in skin carcinoma invasion and vascularization through pharmacological and genetic approaches. [less ▲]

Detailed reference viewed: 35 (4 ULg)
Full Text
Peer Reviewed
See detailQuantification of lipid bilayer effective microviscosity and fluidity effect induced by propofol
Bahri, Mohamed Ali ULg; Heyne, Belinda; Hans, Pol ULg et al

in Biophysical Chemistry (2005), 114(1), 53-61

Electron spin resonance (ESR) spectroscopy with nitroxide spin probes was used as a method to probe the liposome microenvironments. The effective microviscosities have been determined from the calibration ... [more ▼]

Electron spin resonance (ESR) spectroscopy with nitroxide spin probes was used as a method to probe the liposome microenvironments. The effective microviscosities have been determined from the calibration of the ESR spectra of the probes in solvent mixtures of known viscosities. In the first time, by measuring ESR order parameter (S ) and correlation time (s c) of stearic spin probes, we have been able to quantify the value of effective microviscosity at different depths inside the liposome membrane. At room temperature, local microviscosities measured in dimyristoyl-l-a phosphatidylcholine (DMPC) liposome membrane at the different depths of 7.8, 16.95, and 27.7 2 were 222.53, 64.09, and 62.56 cP, respectively. In the gel state (10 °C), those microviscosity values increased to 472.56, 370.61, and 243.37 cP. In a second time, we have applied this technique to determine the modifications in membrane microviscosity induced by 2,6-diisopropyl phenol (propofol; PPF), an anaesthetic agent extensively used in clinical practice. Propofol is characterized by a unique phenolic structure, absent in the other conventional anaesthetics. Indeed, given its lipophilic property, propofol is presumed to penetrate into and interact with membrane lipids and hence to induce changes in membrane fluidity. Incorporation of propofol into dimyristoyl-L-alpha-phosphatidylcholine liposomes above the phase-transition temperature (23.9 °C) did not change microviscosity. At 10 °C, an increase of propofol concentration from 0 to 1.0 10 [less ▲]

Detailed reference viewed: 110 (5 ULg)
See detailQuantification of lipid bilayer effective microviscosity and fluidity effect induced by propofol
Bahri, Mohamed Ali ULg; Heyne, B.; Hans, P. et al

Conference (2004)

Detailed reference viewed: 8 (4 ULg)
See detailQuantification of lipid bilayer viscosity and fuidity effect induced by propofol.
Bahri, Mohamed Ali ULg; Heyne, Belinda; Hans, Pol ULg et al

Conference (2004, April 23)

Detailed reference viewed: 37 (6 ULg)
Full Text
Peer Reviewed
See detailQuantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Veterinary Microbiology (2006), 114(3-4), 318-326

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We ... [more ▼]

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We investigated the nature of the local immune response mounted in canine sino-nasal aspergillosis. Quantitative RT-PCR was carried out on RNA isolated from nasal biopsies from diseased and control dogs, using specific assays designed to amplify mRNA encoding a panel of cytokines and chemokines. Canine sino-nasal aspergillosis was associated with significantly increased expression of mRNA encoding MCP-1, -2, -3 and -4, IL-8, IL-10, EL-18 and TNF-alpha relative to controls (P < 0.01) but there was no difference between groups with respect to IL-4, IL-5, IL-6, IL-12, TGF-beta, and eotaxin-2 and -3. The up-regulation of proinflammatory cytokines and chemokines related to the influx of phagocytic cells might account for the localisation of this infection to the upper respiratory tract. The up-regulation of the expression of the immunomodulatory cytokine IL-10 in nasal tissue from affected dogs might be important in limiting the extent of local tissue destruction, but might also account for the fact that infected dogs are generally unable to clear this infection spontaneously. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 20 (0 ULg)
Peer Reviewed
See detailQuantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis.
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Proceedings of the 15th Annual Congress of the ECVIM-CA (2005)

Detailed reference viewed: 4 (0 ULg)
Peer Reviewed
See detailQuantification of mRNA encoding cytokines, chemokines and CCR3 in bronchial biopsies from dogs with eosinophilic bronchopneumopathy.
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Proceedings of the 15th Annual Congress of the ECVIM-CA (2005)

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailQuantification of pigeon circovirus in serum, blood, semen and different tissues of naturally infected pigeons using a real-time polymerase chain reaction
Duchatel, Jean-Pierre ULg; Todd, D; Willeman, C et al

in Avian Pathology : Journal of the W.V.P.A (2009), 38(2), 143-148

The development of a real-time polymerase chain reaction (PCR) based on SYBR Green chemistry is described for the quantification of pigeon circovirus (PiCV) DNA in various samples. Plasmid containing a ... [more ▼]

The development of a real-time polymerase chain reaction (PCR) based on SYBR Green chemistry is described for the quantification of pigeon circovirus (PiCV) DNA in various samples. Plasmid containing a fragment of the PiCV genome was used to create a standard curve and to estimate the viral DNA copies in analysed samples. Both primers were designed in highly conserved regions to avoid false negatives, and amplified a 139-base-pair amplicon. When the amplifications were performed in the presence of cellular DNA extracted from PCR-negative liver, bursa and spleen samples, the detection limits were respectively 20, 20 and 60 copies of genome per milligram of tissue. These limits were 10, 160 and 25 copies/ml for control blood, sera and semen, respectively. For cloacal swab, the detection limit was 200 copies. The assay showed a <br />linear detection over a six-log range (R2 0.99) and displayed reliable inter-assay and intra-assay reproducibility. Application of the test to sera samples indicated the presence of the virus in Belgium in 1991, 6 years before PiCV infections were histologically diagnosed. Testing of samples from pigeons with <br />‘‘young pigeon sickness’’ showed that the viral loads were high in the bursa of Fabricius (up to 2.07 x 10^9 copies/mg), the liver (up to 2.88x10^8 copies/mg) and spleen (up to 5.57x10^8 copies/mg). For liver, the viral load was significantly higher in sick pigeons than in apparently healthy pigeons. Detection of high quantities of PiCV DNA (up to 1.6x10^9 copies/ml) in the sera or blood of some young healthy pigeons <br />indicated that the viral load in this sample type would not be useful as predictive indicator of disease. This work also showed that PiCV DNA can be detected in relatively large amounts in semen (up to 1.0x10^7 copies/ejaculate) and cloacal swabs (up to 3.6x10^10 copies/swab), confirming that PiCV may be transmitted by vertical and horizontal routes. [less ▲]

Detailed reference viewed: 43 (6 ULg)
Full Text
Peer Reviewed
See detailQuantification of Randomly-methylated-b-cyclodextrin effect on liposome: An ESR study
Grammenos, Angeliki ULg; Bahri, Mohamed Ali ULg; Guelluy, Pierre-Henri ULg et al

in Biochemical and Biophysical Research Communications (2009), 390

In the present work, the effect of Randomly-methylated-b-cyclodextrin (Rameb) on the microviscosity of dimyristoyl-L-a phosphatidylcholine (DMPC) bilayer was investigated using the electron spin resonance ... [more ▼]

In the present work, the effect of Randomly-methylated-b-cyclodextrin (Rameb) on the microviscosity of dimyristoyl-L-a phosphatidylcholine (DMPC) bilayer was investigated using the electron spin resonance (ESR) technique. The ability of Rameb to extract membrane cholesterol was demonstrated. For the first time, the percentage of cholesterol extracted by Rameb from cholesterol doped DMPC bilayer was monitored and quantified throughout a wide Rameb concentration range. The effect of cholesterol on the inner part of the membrane was also investigated using 16-doxyl stearic acid spin label (16-DSA). 16-DSA seems to explore two different membrane domains and report their respective microviscosities. ESR experiments also establish that the presence of 30% of cholesterol in DMPC liposomes suppresses the jump in membrane fluidity at lipids phase-transition temperature (23.9°C). [less ▲]

Detailed reference viewed: 65 (28 ULg)