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See detailPurification, Characterization, and Nucleotide Sequence of the Thermolabile Alpha-Amylase from the Antarctic Psychrotroph Alteromonas Haloplanctis A23
Feller, Georges ULg; Lonhienne, T.; Deroanne, Christophe ULg et al

in Journal of Biological Chemistry (1992), 267(8), 5217-21

The alpha-amylase excreted by the antarctic bacterium Alteromonas haloplanctis was purified and the corresponding amy gene was cloned and sequenced. N- and C-terminal amino acid sequencing were used to ... [more ▼]

The alpha-amylase excreted by the antarctic bacterium Alteromonas haloplanctis was purified and the corresponding amy gene was cloned and sequenced. N- and C-terminal amino acid sequencing were used to establish the primary structure of the mature A. haloplanctis alpha-amylase which is composed of 453 amino acids with a predicted Mr of 49,340 and a pI of 5.5. Three Ca2+ ions are bound per molecule and its activity is modulated by chloride ions. Within the four consensus sequences, Asp-174, Glu-200, and Asp-264 are the proposed catalytic residues. The psychrotrophic A. haloplanctis alpha-amylase is characterized by a high amylolytic activity at low temperatures, a reduced apparent optimal temperature, and typical thermodynamic activation parameters A. haloplanctis alpha-amylase has also a low thermal stability as demonstrated by the temperature effect on both activity and secondary structure. It is suggested that structure flexibility and lower sensitivity of secondary structure to temperature variations in the low temperature range are the main structural adaptations of the psychrotrophic enzyme. The unusual stacking of small amino acids around the catalytic residues is proposed as a factor inducing active site flexibility and concomitant high activity of the enzyme at low temperatures. [less ▲]

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See detailPurification, expansion, and multiple fluorochrome labeling of cord blood hemopoietic precursors: preliminary results.
Feron, C.; Gothot, André ULg; Grosdent, Jean-Claude ULg et al

in Journal of Hematotherapy (1993), 2(2), 259-61

CD34-positive cells were isolated from a total of 23 cords using CellPro Ceprate columns. AIS MicroCellector flasks, and panning. The cells were (1) expanded in serum-free culture supplemented with a ... [more ▼]

CD34-positive cells were isolated from a total of 23 cords using CellPro Ceprate columns. AIS MicroCellector flasks, and panning. The cells were (1) expanded in serum-free culture supplemented with a variety of combinations of cytokines and (2) immunophenotyped using multiple fluorochrome labeling. The results indicated that the avidin column produced the highest purity of CD34-positive cells, and that immature blast cells could be expanded in serum-free culture. Preliminary results suggested that the four fluorochrome labeling technique may provide useful information on the lineage commitment of cord blood precursor and blast cells. [less ▲]

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See detailPurification, refolding and characterization of the trimeric Omp2a outer membrane porin from Brucella melitensis.
Roussel, G; Matagne, André ULg; De Bolle, X et al

in Protein Expression & Purification (2012), 83(2), 198-204

Brucella melitensis is a gram-negative bacteria known to cause brucellosis and to produce severe infections in humans. Whilst brucella's outer membrane proteins have been extensively studied due to their ... [more ▼]

Brucella melitensis is a gram-negative bacteria known to cause brucellosis and to produce severe infections in humans. Whilst brucella's outer membrane proteins have been extensively studied due to their potential role as antigens or virulence factors, their function is still poorly understood at the structural level, as the 3D structure of Brucella beta-barrel membrane proteins are still unknown. In this context, the B. melitensis trimeric Omp2a porin has been overexpressed and refolded in n-dodecyl-beta-d-maltopyranoside. We here show that this refolding process is insensitive to urea but is temperature- and ionic strength-dependent. Reassembled species were characterized by fluorescence, size-exclusion chromatography and circular dichroism. A refolding mechanism is proposed, suggesting that Omp2a first refolds under a monomeric form and then self-associates into a trimeric state. This first complete in vitro refolding of a membrane protein from B. melitensis shall eventually lead to functional and 3D structure determination. [less ▲]

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See detailPurificazione delle proteine associate alla gestazione (PAG) nella bufala (Bubalus bubalis): risultati preliminari.
Barbato, O.; Melo de Sousa, Noelita ULg; Malfatti, A. et al

in Atti 2o Congresso Nazionale sull’Allevamento del Bufalo (2003)

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See detailPurified FSH supplemented with defined amounts of LH for superovulation in dairy cattle .
Herrler, A.; Beckers, Jean-François ULg; Viviers-Donnay, I. et al

in Theriogenology (1988, January), 29(1), 260

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See detailLe purisme enfantin au prisme du discours spontané
Meunier, Deborah ULg

in Français Moderne (Le) (2008), LXXXVI/1

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See detailPurpose and scope
Abramowucz, Daniel; Cochat, Pierre; Claas, Frans et al

in Nephrology Dialysis Transplantation (2013), 28

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See detailPurpura thrombotique thrombocytopénique
Dokekias, A. E.; Croisiaux, C.; Andrien, F. et al

in Revue Médicale de Liège (1989), 44

Deux patientes âgées respectivement de 58 et 24 ans ont été hospitalisées dans le Service d'Hématologie du CHU Sart Tilman pour PTT. Le diagnostic a été établi à partir de certains symptômes classiques à ... [more ▼]

Deux patientes âgées respectivement de 58 et 24 ans ont été hospitalisées dans le Service d'Hématologie du CHU Sart Tilman pour PTT. Le diagnostic a été établi à partir de certains symptômes classiques à savoir : hémolyse intravasculaire avec schizocytose (40/1000 dans le premier cas et 30/1000 dans le deuxième), élévation importante des LDH, thrombocytopénie majeure < 30.000/mm³, signes de souffrance du système nerveux central et insuffisance rénale aiguë dans le premier cas. Le scanner abdominal a permis d'observer dans le premier cas (admis à un stade avancé) un infarctus splénique massif et dans les deux cas une augmentation des indices de résistivité artérielle, témoignant d'une souffrance rénale. Le traitement par échanges plasmatiques a été instauré avec succès. Après obtention de la rémission complète, des infusions de plasma frais ont été poursuivies et le traitement d'entretien a été relayé par les antiagrégants plaquettaires. [less ▲]

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See detailPushing the precision limit of ground-based eclipse photometry
Gillon, Michaël ULg; Anderson, D. R.; Demory, B *-O et al

Report (2008)

Until recently, it was considered by many that ground-based photometry could not reach the high cadence sub-mmag regime because of the presence of the atmosphere. Indeed, high frequency atmospheric noises ... [more ▼]

Until recently, it was considered by many that ground-based photometry could not reach the high cadence sub-mmag regime because of the presence of the atmosphere. Indeed, high frequency atmospheric noises (mainly scintillation) limit the precision that high SNR photometry can reach within small time bins. If one is ready to damage the sampling of his photometric time-series, binning the data (or using longer exposures) allows to get better errors, but the obtained precision will be finally limited by low frequency noises. To observe several times the same planetary eclipse and to fold the photometry with the orbital period is thus generally considered as the only option to get very well sampled and precise eclipse light curve from the ground. Nevertheless, we show here that reaching the sub-mmag sub-min regime for one eclipse is possible with a ground-based instrument. This has important implications for transiting planets characterization, secondary eclipses measurement and small planets detection from the ground. [less ▲]

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See detailPustulose exanthématique aiguë généralisée due à un agent de contraste iodé pour radiodiagnostic.
Paquet, Philippe ULg; Vandenbossche, Géraldine; Nikkels, Arjen ULg et al

in Revue Médicale de Liège (2009), 64

Iodinated contrast agents are frequently involved in delayed polymorphic adverse skin reactions. Acute generalized exanthematous pustulosis following administration of iodinated contrast agents is a rare ... [more ▼]

Iodinated contrast agents are frequently involved in delayed polymorphic adverse skin reactions. Acute generalized exanthematous pustulosis following administration of iodinated contrast agents is a rare but severe form of such reactions. The disease is characterized by the sudden occurrence of an erosive and pustular erythroderma with fever, leukocytosis and sometimes peripheral adenopathies and liver involvement. This condition is considered as an immunologic reaction, primarily involving T lymphocytes. The overall mortality reaches about 1%. Elucidating the differential diagnosis with other acute paroxysmal drug eruptions (toxic epidermal necrolysis, Steven-Johnson syndrome and drug hypersensitivity syndrome) is of paramount importance for establishing the adequate treatment of PEAG. [less ▲]

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See detailLa pustulose sous-cornee de Sneddon et Wilkinson.
Martalo, O.; Paquet, Philippe ULg; Pierard, Gérald ULg

in Revue Médicale de Liège (2003), 58(10), 628-30

The subcorneal pustular dermatosis of Sneddon and Wilkinson belongs to the heterogeneous group of neutrophilic dermatosis. This unique disorder is characterized by a superficial pustular eruption. The ... [more ▼]

The subcorneal pustular dermatosis of Sneddon and Wilkinson belongs to the heterogeneous group of neutrophilic dermatosis. This unique disorder is characterized by a superficial pustular eruption. The pustules are flaccid and aseptic. They develop predominantly on the trunk and in the groins, axillae and submammary areas. This disease is benign and chronic. However, it can be associated with some other conditions such as lymphoproliferative and myeloproliferative diseases. Dapsone is the treatment of choice to control the skin manifestations. [less ▲]

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See detail"Putain" de Nelly Aran, une écriture scandaleusement intime
Hick, Anne-Laure ULg

in Bertrand, Jean-Pierre; Frederic, Madeleine; den Toonder, Jeanette (Eds.) Écritures de l’intime dans la littérature francophone du Canada (1980-2005) (in press)

Récit, roman, autofiction, autobiographie, roman autobiographique… qu’il s’agisse des éditeurs, des critiques, du monde universitaire ou de l’auteur elle-même, personne ne s’accorde lorsqu’il s’agit de ... [more ▼]

Récit, roman, autofiction, autobiographie, roman autobiographique… qu’il s’agisse des éditeurs, des critiques, du monde universitaire ou de l’auteur elle-même, personne ne s’accorde lorsqu’il s’agit de qualifier Putain (2001) de Nelly Arcan. En effet, au-delà du scandale thématique de l’œuvre, le mécanisme énonciatif de celle-ci est très particulier. Il s’agira dans cet article de montrer comment l’écriture de ce récit intime fuit tout classement selon les règles des genres autobiographiques, tout en exploitant celles-ci de façon tout à fait originale – et donc scandaleuse. [less ▲]

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See detailPutative DNA G-quadruplex formation within the promoters of Plasmodium falciparum var genes
Smargiasso, Nicolas ULg; Gabelica, Valérie ULg; Damblon, Christian ULg et al

in BMC Genomics (2009), 10

Background. Guanine-rich nucleic acid sequences are capable of folding into an intramolecular four-stranded structure called a G-quadruplex. When found in gene promoter regions, G-quadruplexes can ... [more ▼]

Background. Guanine-rich nucleic acid sequences are capable of folding into an intramolecular four-stranded structure called a G-quadruplex. When found in gene promoter regions, G-quadruplexes can downregulate gene expression, possibly by blocking the transcriptional machinery. Here we have used a genome-wide bioinformatic approach to identify Putative G-Quadruplex Sequences (PQS) in the Plasmodium falciparum genome, along with biophysical techniques to examine the physiological stability of P. falciparum PQS in vitro. Results. We identified 63 PQS in the non-telomeric regions of the P. falciparum clone 3D7. Interestingly, 16 of these PQS occurred in the upstream region of a subset of the P. falciparum var genes (group B var genes). The var gene family encodes PfEMP1, the parasite’s major variant antigen and adhesin expressed at the surface of infected erythrocytes, that plays a key role in malaria pathogenesis and immune evasion. The ability of the PQS found in the upstream regions of group B var genes (UpsB-Q) to form stable Gquadruplex structures in vitro was confirmed using 1H NMR, circular dichroism, UV spectroscopy, and thermal denaturation experiments. Moreover, the synthetic compound BOQ1 that shows a higher affinity for DNA forming quadruplex rather than duplex structures was found to bind with high affinity to the UpsB-Q. Conclusions. This is the first demonstration of non-telomeric PQS in the genome of P. falciparum that form stable G-quadruplexes under physiological conditions in vitro. These results allow the generation of a novel hypothesis that the G-quadruplex sequences in the upstream regions of var genes have the potential to play a role in the transcriptional control of this major virulence-associated multi-gene family. [less ▲]

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See detailPutative glacial refugia of Cedrus atlantica deduced from Quaternary pollen records and modern genetic diversity
Cheddadi, R.; Fady, B.; François, Louis ULg et al

in Journal of Biogeography (2009), 36(7), 1361-1371

Aim To investigate the impact of past environmental changes on Cedrus atlantica and its current genetic diversity, and to predict its future distribution. Location Morocco, Algeria and Tunisia. Methods ... [more ▼]

Aim To investigate the impact of past environmental changes on Cedrus atlantica and its current genetic diversity, and to predict its future distribution. Location Morocco, Algeria and Tunisia. Methods Eleven fossil pollen records from these three countries were used to locate putative glacial refugia and to reconstruct past climate changes. A mechanistic vegetation distribution model was used to simulate the distribution of C. atlantica in the year 2100. In addition, a genetic survey was carried out on modern Moroccan C. atlantica. Results Pollen records indicate that Cedrus was present during the last glacial period, probably in scattered refugia, in Tunisia, Algeria and Morocco. In the Tunisian and Algerian sites, cedar expanded during the late glacial and the early Holocene, then disappeared after c. 8000 yr bp. Reconstructed mean annual precipitation and January temperature show that the last glacial period in Morocco was cooler by 10-15 degrees C and drier by c. 300-400 mm year(-1) than the climate today. Modern chloroplast microsatellites of 15 C. atlantica populations in Morocco confirm the presence of multiple refugia and indicate that cedar recolonized the Moroccan mountains fairly recently. Model simulation indicates that by the year 2100 the potential distribution of C. atlantica will be much restricted with a potential survival area located in the High Atlas. Main conclusions Environmental changes in northern Africa since the last glacial period have had an impact on the geographical distribution of C. atlantica and on its modern genetic diversity. It is possible that by the end of this century C. atlantica may be unable to survive in its present-day locations. To preserve the species, we suggest that seedlings from modern C. atlantica populations located in the High Atlas mountains, where a high genetic diversity is found, be transplanted into the western High Atlas. [less ▲]

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See detailPutative implication of equine herpesvirus in lower airway inflammation: systematic PCR assays of 708 respiratory fluids
Fortier, Guillaume ULg; Richard, Eric ULg; Pronost, S. et al

in Tessier, Caroline; Gerber, Vincent (Eds.) Abstract book: 4th World Equine Airways Symposium (WEAS) (2009)

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See detailPutative roles of the CNF2 and CDTIII toxins in experimental infections with necrotoxigenic Escherichia coli type 2 (NTEC2) strains in calves
Van Bost, S.; Roels, S.; Oswald, E. et al

in Microbes & Infection (2003), 5(13), 1189-1193

Newborn colostrum-restricted calves were orally inoculated with an Escherichia coli strain, identified originally as non-pathogenic, and into which the plasmid pVir was conjugally transferred. This ... [more ▼]

Newborn colostrum-restricted calves were orally inoculated with an Escherichia coli strain, identified originally as non-pathogenic, and into which the plasmid pVir was conjugally transferred. This resulted in diarrhea, intestinal lesions and extra-intestinal invasion, suggesting that factors affecting these pathogenic properties are located on pVir. In order to analyze the respective roles of the toxins CNF2 and CDTIII in the pathogenesis, colostrum-restricted calves were inoculated with isogenic mutants in the cnf2 and the cdt-III genes. The loss of cnf2 is associated with a reduction in the pathogenicity, since diarrhea does not occur in calves challenged, in spite of successful colonization of the intestine. Nevertheless, the mutant strain remains able to invade the bloodstream and to localize in the internal organs. Conversely, the calves inoculated with mutant in the cdt-III gene evolved in the same way as wild-type strain-inoculated calves with regard to clinical signs and macroscopic or microscopic lesions. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved. [less ▲]

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See detailPutois, écureuil, hérisson, loir
Libois, Roland ULg

Learning material (1992)

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See detailPutrescine metabolism in a fully habituated nonorganogenic sugar beet callus and its relationship with growth
Kevers, Claire ULg; Bisbis, Badia; Faivre-Rampant, Odile et al

in Journal of Plant Physiology (1999), 154(4), 503-508

A fully-habituated and nonorganogenic (HNO) sugar beet callus was previously shown to overproduce polyamines, as compared with a normal (N) auxin- and cytokinin-dependent callus of the same strain ... [more ▼]

A fully-habituated and nonorganogenic (HNO) sugar beet callus was previously shown to overproduce polyamines, as compared with a normal (N) auxin- and cytokinin-dependent callus of the same strain. Because relationships were established between polyamine levels and metabolism with different growth and development processes, some key enzymes in the metabolic pathways of polyamines were investigated in the HNO callus, and their involvement in growth appraised. Putrescine was found to be the major free and conjugated polyamine in the HNO callus. It was biosynthesised preferentially via ornithine and ornithine decarboxylase (ODC), which is in agreement with the surplus of synthesised ornithine. Diamine (DAO) and polyamine (PAO)-oxidase activities were also highest in the HNO callus, as compared with the normal, with DAO being the more active. Transglutaminase activities (+/- Ca) were also higher in HNO than in normal callus. Addition of different polyamines or of inhibitors of their biosynthesis to the culture medium of the HNO callus modified the level of endogenous polyamines and affected callus growth. The results thus pointed out a higher polyamine metabolism, particularly of putrescine, in the actively growing auxin- and cytokinin-independent callus than in the normal one. They also provided evidence for the sensitivity of a habituated tissue type towards this class of growth regulators. [less ▲]

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See detailPutting Prebiotics in their Place. On Europe's Intestinal Flora
Hendrickx, Kim ULg

Scientific conference (2012, January 18)

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