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See detailNucleotide sequence of the gene encoding the active-site serine β-lactamase from Streptomyces cacaoi
Lenzini, Mauro V.; Ishihara, Hiroshi; Dusart, Jean et al

in FEMS Microbiology Letters (1988), 49(3), 371-376

The gene encoding the extracellular active-site serine β-lactamase of Streptomyces cacaoi previously cloned into Streptomyces lividans, has the information for the synthesis of a 303 amino-acid precursor ... [more ▼]

The gene encoding the extracellular active-site serine β-lactamase of Streptomyces cacaoi previously cloned into Streptomyces lividans, has the information for the synthesis of a 303 amino-acid precursor. The β-lactamase as excreted by the host S. lividans ML1, has a ragged amino-terminus, indicating either the presence of a leader peptidase of poor specificity or the action of an amino-peptidase. The deduced primary structure has been confirmed by amino acid sequencing of a 10-residue stretch at the amino terminus of the mature protein and an 8-residue stretch containing the active-site serine. The S. cacaoiβ-lactamase is highly homologous with the class A β-lactamases of Streptomyces albus G and Staphylococcus aureus of known three-dimensional structure. Amino acid alignments show that the S. cacaoiβ-lactamase essentially differs from these two latter enzymes by short insertions and deletions that do not affect the spatial disposition of the secondary structures. [less ▲]

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See detailNucleotide sequence of the gene encoding the Streptomyces albus G β-lactamase precursor
Dehottay, Philippe; Dusart, Jean; De Meester, Fabien et al

in European Journal of Biochemistry (1987), 166(2), 345-350

A 1400-base DNA fragment, which contains the gene encoding the extracellular active-site serine beta-lactamase of Streptomyces albus G previously cloned into Streptomyces lividans [Dehottay et al. (1986 ... [more ▼]

A 1400-base DNA fragment, which contains the gene encoding the extracellular active-site serine beta-lactamase of Streptomyces albus G previously cloned into Streptomyces lividans [Dehottay et al. (1986) Gene 42, 31-36], was sequenced. The gene codes for a 314-amino-acid precursor, the N-terminal region of which has the characteristics of a signal peptide. The beta-lactamase as excreted by the host strain S. lividans PD6 has a ragged N-terminus, indicating either the presence of a leader peptidase of poor specificity or the action of an aminopeptidase. The primary structure (as deduced from the nucleotide sequence) was confirmed by amino acid sequencing of a 16-residue stretch at the amino terminus of the protein, a 12-residue stretch containing the active-site serine [De Meester et al. (1987) Biochem. J. 244, 427-432] and a 23-residue stretch obtained by trypsin digestion of the protein. The beta-lactamase belongs to class A, has three half-cystine residues (one of which occurs on the amino side of the active-site serine) and is inactivated by thiol reagents. Putative ribosome binding site and terminator region were identified. [less ▲]

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See detailNucleotide Sequence of the Lipase Gene Lip2 from the Antarctic Psychrotroph Moraxella Ta144 and Site-Specific Mutagenesis of the Conserved Serine and Histidine Residues
Feller, Georges ULg; Thiry, M.; Gerday, Charles ULg

in DNA & Cell Biology (1991), 10(5), 381-8

The lip2 gene from the antarctic psychotroph Moraxella TA144 was sequenced. The primary structure of the Lip2 preprotein deduced from the nucleotide sequence is composed of 433 amino acids with a ... [more ▼]

The lip2 gene from the antarctic psychotroph Moraxella TA144 was sequenced. The primary structure of the Lip2 preprotein deduced from the nucleotide sequence is composed of 433 amino acids with a predicted Mr of 47,222. This enzyme contains a Ser-centered consensus sequence and a conserved His-Gly dipeptide found in most lipase amino-terminal domains. These sequences are involved in the lipase active site conformation since substitution of the conserved Ser or His residues by Ala and Gln, respectively, results in the loss of both lipase and esterase activities. Structural factors that would allow proper enzyme flexibility at low temperatures are discussed. It is suggested that only subtle changes in the primary structure of these psychrotrophic enzymes can account for their ability to catalyze lipolysis at temperatures close to 0 degrees C. [less ▲]

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See detailNucleotide Sequence of the Lipase Gene Lip3 from the Antarctic Psychotroph Moraxella Ta144
Feller, Georges ULg; Thiry, M.; Gerday, Charles ULg

in Biochimica et Biophysica Acta (1991), 1088(2), 323-4

A lipase gene (lip3) from the psychotrophic strain Moraxella TA144 has been cloned and sequenced. The deduced primary structure of the lipase preprotein is composed of 315 amino acids with a predicted Mr ... [more ▼]

A lipase gene (lip3) from the psychotrophic strain Moraxella TA144 has been cloned and sequenced. The deduced primary structure of the lipase preprotein is composed of 315 amino acids with a predicted Mr of 34,772. This enzyme contains two consensus peptides showing cluster of glycine residues that may be involved in domain flexibility. The cloned gene product conserves the low temperature activity and the thermolability properties of the wild enzyme. [less ▲]

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See detailNucleotide sequence of the ovine P53 tumor-suppressor cDNA and its genomic organization.
Dequiedt, Franck ULg; Kettmann, Richard ULg; Burny, A. et al

in DNA Sequence : The Journal of DNA Sequencing & Mapping (1995), 5(4),

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See detailNucleotide sequence of the proline permease gene PUT4 of Saccharomyces cerevisiae.
Vandenbol, Micheline ULg; Hidalgo, E.; Vissers, S. et al

Poster (1987)

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See detailNucleotide sequences of the pbpX genes encoding the penicillin-binding proteins 2x from Streptococcus pneumoniae R6 and a cefotaxime-resistant mutant, C506
Laible, G.; Hakenbeck, R.; Sicard, M. A. et al

in Molecular Microbiology (1989), 3(10), 1337-1348

Development of penicillin resistance in Streptococcus pneumoniae is due to successive mutations in penicillin-binding proteins (PBPs) which reduce their affinity for beta-lactam antibiotics. PBP2x is one ... [more ▼]

Development of penicillin resistance in Streptococcus pneumoniae is due to successive mutations in penicillin-binding proteins (PBPs) which reduce their affinity for beta-lactam antibiotics. PBP2x is one of the high-Mr PBPs which appears to be altered both in resistant clinical isolates, and in cefotaxime-resistant laboratory mutants. In this study, we have sequenced a 2564 base-pair chromosomal fragment from the penicillin-sensitive S. pneumoniae strain R6, which contains the PBP2x gene. Within this fragment, a 2250 base-pair open reading frame was found which coded for a protein having an Mr of 82.35kD, a value which is in good agreement with the Mr of 80-85 kD measured by SDS-gel electrophoresis of the PBP2x protein itself. The N-terminal region resembled an unprocessed signal peptide and was followed by a hydrophobic sequence that may be responsible for membrane attachment of PBP2x. The corresponding nucleotide sequence of the PBP2x gene from C504, a cefotaxime-resistant laboratory mutant obtained after five selection steps, contained three nucleotide substitutions, causing three amino acid alterations within the beta-lactam binding domain of the PBP2x protein. Alterations affecting similar regions of Escherichia coli PBP3 and Neisseria gonorrhoeae PBP2 from beta-lactam-resistant strains are known. The penicillin-binding domain of PBP2x shows highest homology with these two PBPs and S. pneumoniae PBP2b. In contrast, the N-terminal extension of PBP2x has the highest homology with E. coli PBP2 and methicillin-resistant Staphylococcus aureus PBP2'. No significant homology was detected with PBP1a or PBP1b of Escherichia coli, or with the low-Mr PBPs. [less ▲]

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See detailNude athymic syndrom in a calf
Leroy, Pascal ULg; Hanset, R.; Doizé, Cécile ULg

(1980)

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See detailNudge and the Law: A European Perspective
Sibony, Anne-Lise ULg; Alemanno, Alberto

Book published by Hart Publishing (2015)

Behavioural sciences provide a better understanding of human decision-making. Increasingly, governments around the world are keen to rely on these insights for reshaping public interventions in a wide ... [more ▼]

Behavioural sciences provide a better understanding of human decision-making. Increasingly, governments around the world are keen to rely on these insights for reshaping public interventions in a wide range of policy areas such as energy, health, financial services and data protection. When policy-making meets behavioural sciences, effective, low- cost and choice preserving regulations can emerge in the form of default rules, smart disclosure and simplification requirements. While behaviourally-informed regulation has a huge potential, it also attracts legitimacy and practicability concerns. Nudge and the Law explores the legal implications of the emergent phenomenon of behavioural regulation by focusing on the challenges and opportunities it may offer to EU policy-making and beyond [less ▲]

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See detailNudging acne by topical beta-lipohydroxy acid (LHA), a new comedolytic agent.
Pierard, Gérald ULg; Rougier, A.

in European Journal of Dermatology (2002), 12(4), -

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See detailNudging epidermal field cancerogenesis by imiquimod.
Uhoda, Isabelle; Quatresooz, Pascale ULg; Pierard, Claudine ULg et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (2003), 206(4), 357-60

BACKGROUND: The coexistence of malignancies close to each other on the skin has been occasionally reported. The concept of field cancerogenesis applies to such cases. Given the purported mechanism of ... [more ▼]

BACKGROUND: The coexistence of malignancies close to each other on the skin has been occasionally reported. The concept of field cancerogenesis applies to such cases. Given the purported mechanism of action of imiquimod, it should not be surprising that this treatment could inhibit epidermal field cancerogenesis. AIM: To assess the effect of imiquimod applied twice weekly on incipient bowenoid changes disclosed in the vicinity of basal cell carcinomas. MATERIALS AND METHOD: Biopsies were taken before treatment and after 4-6 weeks and 12 weeks of imiquimod treatment. RESULTS: Large atypical bowenoid keratinocytes and dyskeratotic cells were cleared in time while factor-XIIIa-positive dermal dendrocytes appeared boosted and admixed with a brisk lymphocytic infiltration. CONCLUSION: Epidermal field cancerogenesis appears to be controlled by imiquimod. Dermal dendrocytes might play a pivotal role in this regression phenomenon. [less ▲]

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See detailNudging hair shedding by antidandruff shampoos. A comparison of 1% ketoconazole, 1% piroctone olamine and 1% zinc pyrithione formulations.
Franchimont, Claudine ULg; Goffin, Véronique ULg; Henry, Frédérique ULg et al

in International Journal of Cosmetic Science (2002), 24(5), 249-56

Hair shedding and hair thinning have been reported to be affected by dandruff and seborrhoeic dermatitis. The present study was conducted in 150 men presenting with telogen effluvium related to androgenic ... [more ▼]

Hair shedding and hair thinning have been reported to be affected by dandruff and seborrhoeic dermatitis. The present study was conducted in 150 men presenting with telogen effluvium related to androgenic alopecia associated with dandruff. They were randomly allocated to three groups receiving each one of the three shampoos in the market containing either 1% ketoconazole (KTZ), 1% piroctone olamine (PTO) or 1% zinc pyrithione (ZPT). Shampoos had to be used 2-3 times a week for 6 months. Hair shedding during shampoo was evaluated semiquantitatively. Hair density on the vertex was evaluated on photographs using a Dermaphot. Trichograms were used for determining the anagen hair percentage and the mean proximal hair shaft diameter using computerized image analysis. The sebum excretion rate (SER, mug cm(-2) h(-1)) was also measured using a Sebumeter((R)). The three treatments cleared pruritus and dandruff rapidly. At end point, hair density was unchanged, although hair shedding was decreased (KTZ: -17.3%, PTO: -16.5%, ZPT: -10.1%) and the anagen hair percentage was increased (KTZ: 4.9%, PTO: 7.9%, ZPT: 6.8%). The effect on the mean hair shaft diameter was contrasted between the three groups of volunteers (KTZ: 5.4%, PTO: 7.7%, ZPT: -2.2%). In conclusion, telogen effluvium was controlled by KTZ, PTO and ZPT shampoos at 1% concentration. In addition, KTZ and PTO increased the mean hair shaft thickness while discretely decreasing the sebum output at the skin surface. [less ▲]

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See detailNudus... infans... A propos de Lucrèce, V, 222-227
Rochette, Bruno ULg

in Etudes Classiques (Namur, Belgium) (1992), 60

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See detailNueva mutacion de AIP en adenomas hipofisarios familiares (FIPA)
Bilbao, I.; Daly, A. F.; Alvarez Coca, M. et al

in Abstract book - XIV congreso de la Sociedad de Endocrinologia, diabetes y nutricion de Euskadi (2011, March)

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See detailLa nueva novela hispanoamericana. Boom y bumerang
Vanden Berghe, Kristine ULg

Master's dissertation (1987)

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See detailNueva visión en las relaciones médico-paciente, de la Prevención Cuaternaria a la Actitud Cuaternaria.
Jamoulle, Marc ULg

Conference (2015, March 21)

Allocution plénière finale du congrès de Montevideo de la Wonca CIMF Description du concept de prévention quaternaire. Annonce de la parution et présentation des auteurs du numéro spécial de la revue ... [more ▼]

Allocution plénière finale du congrès de Montevideo de la Wonca CIMF Description du concept de prévention quaternaire. Annonce de la parution et présentation des auteurs du numéro spécial de la revue Brésilienne de médecin de famille et communautaire (RBMFC) qui va paraitre incessamment sur ce thème. Annonce de la création par les représentants des associations de médecine de famille de 15 pays, au sein de la branche ibéro américaine de la Wonca (Wonca CIMF), d'un groupe d'intérêt sur la prévention quaternaire [less ▲]

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See detailUn nuevo enfoque sobre la epidemioloiga de las onicomicosis.
Arrese Estrada, Jorge ULg; Valverde, J.; Pierard, Gérald ULg

in Revista Iberoamericana de Micología (2005), 22

Onychomycoses represent a group of nail affections caused by one or more fungi. Whether aging represents a risk factor for developing onychomycosis, remains a question. In the present work, we studied the ... [more ▼]

Onychomycoses represent a group of nail affections caused by one or more fungi. Whether aging represents a risk factor for developing onychomycosis, remains a question. In the present work, we studied the variations in frequency and prevalence of onychomycoses and non-mycotic onychodystrophies according to age. Our results show that there is an increase in the frequency and prevalence of onychomycoses and non-mycotic onychodystrophies predominantly in patients over forty years of age. [less ▲]

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See detailNuevos registros paleobotánicos del Cretácico inferior de la cuenca de Cameros occidental (provincia de Burgos)
Sender, Luis Miguel; Villanueva-Amadoz, Uxue; Diez, José B. et al

in Geogaceta (2010), 50(2), 181-184

Palaeobotanical records of the Cameros basin are scarce and limited to a few localities. Two new records , mainly corresponding to Barremian deposits near Salas de los Infantes and Hortezuelos (Burgos ... [more ▼]

Palaeobotanical records of the Cameros basin are scarce and limited to a few localities. Two new records , mainly corresponding to Barremian deposits near Salas de los Infantes and Hortezuelos (Burgos) are presented. The new flora from Horcajuelos-1 near Salas corresponds to fluvial deposits of the Pinilla de los Moros Fm. A lower bed is dominated by Tempskya, while an upper layer includes abundant Onychiopsis cf. psilotoides, Regnellites, Ruffordia and Cladophlebis. The palynological records of the laterally equivalent lacustrine facies from the Hortezuelos Fm. are dominated by abundant and diverse fern spore assemblages (mainly Schizaeaceae) along with conifers (Classopollis and Araucariacites) and rare monosulcate angiosperm pollen. [less ▲]

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See detailLos nuevos trabajadores migrantes en Europa. El surgimiento de una política común de inmigración laboral.
Gsir, Sonia ULg

in Revista Latina de Sociología (2013), 3

Since the zero-immigration discourse dominated, the European cooperation on labour immigration did not develop as one of free movement of Community workers. Discussing about a common policy on new labour ... [more ▼]

Since the zero-immigration discourse dominated, the European cooperation on labour immigration did not develop as one of free movement of Community workers. Discussing about a common policy on new labour immigration in Europe has not been an easy process. A policy plan was finally adopted six years after the implementation of the Amsterdam Treaty in 1999 to reach a first law in 2009. The first aim of this paper is to understand the policy-making process of a common labour immigration policy. Based on the theoretical framework of discursive institutionalism, this paper aims to explain the policy process carried out by the European actors, particularly the European Commission. Using the concept of discourse defined as ideas and interactions, it examines the ideas about new labour migration in Europe. It shows how the European actors reached step by step an agreement on the so-called Blue Card Directive. The migrant workers categories emerging from the process range from the new economic migrant to the emblematic highly skilled worker. The common labour migration policy that was finally adopted leaves the Community door ajar. It is characterized by a differentiated approach and a preference for temporary immigration. [less ▲]

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