Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailRegulation of procollagen amino-propeptide processing during mouse embryogenesis by specialization of homologous ADAMTS proteases: insights on collagen biosynthesis and dermatosparaxis
Le Goff, Carine; Somerville, Robert PT; Kesteloot, Frédéric ULg et al

in Development (2006), 133(8), 1587-1596

Mutations in ADAMTS2, a procollagen amino-propeptidase, cause severe skin fragility, designated as dermatosparaxis in animals, and a subtype of the Ehlers-Danlos syndrome (dermatosparactic type or VIIC ... [more ▼]

Mutations in ADAMTS2, a procollagen amino-propeptidase, cause severe skin fragility, designated as dermatosparaxis in animals, and a subtype of the Ehlers-Danlos syndrome (dermatosparactic type or VIIC) in humans. Not all collagen-rich tissues are affected to the same degree, which suggests compensation by the ADAMTS2 homologs ADAMTS3 and ADAMTS14. In situ hybridization of Adamts2, Adamts3 and Adamts14, and of the genes encoding the major. brillar collagens, Col1a1, Col2a1 and Col3a1, during mouse embryogenesis, demonstrated distinct tissue-specific, overlapping expression patterns of the protease and substrate genes. Adamts3, but not Adamts2 or Adamts14, was co-expressed with Col2a1 in cartilage throughout development, and with Col1a1 in bone and musculotendinous tissues. ADAMTS3 induced procollagen I processing in dermatosparactic. broblasts, suggesting a role in procollagen I processing during musculoskeletal development. Adamts2, but not Adamts3 or Adamts14, was co-expressed with Col3a1 in many tissues including the lungs and aorta, and Adamts2(-/-) mice showed widespread defects in procollagen III processing. Adamts2(-/-) mice had abnormal lungs, characterized by a decreased parenchymal density. However, the aorta and collagen fibrils in the aortic wall appeared normal. Although Adamts14 lacked developmental tissue-specific expression, it was co-expressed with Adamts2 in mature dermis, which possibly explains the presence of some processed skin procollagen in dermatosparaxis. The data show how evolutionarily related proteases with similar substrate preferences may have distinct biological roles owing to tissue specific gene expression, and provide insights into collagen biosynthesis and the pathobiology of dermatosparaxis. [less ▲]

Detailed reference viewed: 43 (11 ULg)
Peer Reviewed
See detailRegulation of prolactin gene expression in fishes
Poncelet, A. C.; Yaron, Z.; Levavi-Sivan, B. et al

in Recent advances in marine biotechnology (1996)

Detailed reference viewed: 15 (2 ULg)
Full Text
See detailRegulation of Radial Glia Phenotype
Leprince, Pierre ULg; Chanas-Sacre, Grazyna

Part of book (2001)

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailRegulation of subchondral bone osteoblast metabolism by cyclic compression.
Sanchez, Christelle ULg; Pesesse, Laurence ULg; Gabay, Odile et al

in Arthritis and Rheumatism (2012), 64(4), 1193-203

OBJECTIVE: Recent data have shown that abnormal subchondral bone remodeling plays an important role in osteoarthritis (OA) onset and progression, and it was suggested that abnormal mechanical pressure ... [more ▼]

OBJECTIVE: Recent data have shown that abnormal subchondral bone remodeling plays an important role in osteoarthritis (OA) onset and progression, and it was suggested that abnormal mechanical pressure applied to the articulation was responsible for these metabolic changes. This study was undertaken to evaluate the effects of cyclic compression on osteoblasts from OA subchondral bone. METHODS: Osteoblasts were isolated from sclerotic and nonsclerotic areas of human OA subchondral bone. After 28 days, the osteoblasts were surrounded by an abundant extracellular matrix and formed a resistant membrane, which was submitted to cyclic compression (1 MPa at 1 Hz) for 4 hours. Gene expression was evaluated by reverse transcription-polymerase chain reaction. Protein production in culture supernatants was quantified by enzyme-linked immunosorbent assay or visualized by immunohistochemistry. RESULTS: Compression increased the expression of genes coding for interleukin-6 (IL-6), cyclooxygenase 2, RANKL, fibroblast growth factor 2, IL-8, matrix metalloproteinase 3 (MMP-3), MMP-9, and MMP-13 but reduced the expression of osteoprotegerin in osteoblasts in both sclerotic and nonsclerotic areas. Colalpha1(I) and MMP-2 were not significantly affected by mechanical stimuli. Nonsclerotic osteoblasts were significantly more sensitive to compression than sclerotic ones, but after compression, differences in messenger RNA levels between nonsclerotic and sclerotic osteoblasts were largely reduced or even abolished. Under basal conditions, sclerotic osteoblasts expressed similar levels of alpha5, alphav, beta1, and beta3 integrins and CD44 as nonsclerotic osteoblasts but 30% less connexin 43, an important mechanoreceptor. CONCLUSION: Genes involved in subchondral bone sclerosis are mechanosensitive. After compression, nonsclerotic and sclerotic osteoblasts expressed a similar phenotype, suggesting that compression could be responsible for the phenotype changes in OA subchondral osteoblasts. [less ▲]

Detailed reference viewed: 29 (1 ULg)
Full Text
See detailRegulation of the activity of monoaminergic neurons by ion channels : an opportunity for new therapeutic approaches ?
Seutin, Vincent ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (2008), 163(5), 213-22

Detailed reference viewed: 27 (14 ULg)
Full Text
Peer Reviewed
See detailRegulation of the alternative oxidase Aox1 gene in Chlamydomonas reinhardtii. Role of the nitrogen source on the expression of a reporter gene under the control of the Aox1 promoter
Baurain, Denis ULg; Dinant, Monique; Coosemans, Nadine ULg et al

in Plant Physiology (2003), 131(3), 1418-1430

In higher plants, various developmental and environmental conditions enhance expression of the alternative oxidase (AOX), whereas its induction in fungi is mainly dependent on cytochrome pathway ... [more ▼]

In higher plants, various developmental and environmental conditions enhance expression of the alternative oxidase (AOX), whereas its induction in fungi is mainly dependent on cytochrome pathway restriction and triggering by reactive oxygen species. The AOX of the unicellular green alga Chlamydomonas reinhardtii is encoded by two different genes, the Aox1 gene being much more transcribed than Aox2. To analyze the transcriptional regulation of Aox1, we have fused its 1.4-kb promoter region to the promoterless arylsulfatase (Ars) reporter gene and measured ARS enzyme activities in transformants carrying the chimeric construct. We show that the Aox1 promoter is generally unresponsive to a number of known AOX inducers, including stress agents, respiratory inhibitors, and metabolites, possibly because the AOX activity is constitutively high in the alga. In contrast, the Aox1 expression is strongly dependent on the nitrogen source, being down-regulated by ammonium and stimulated by nitrate. Inactivation of nitrate reductase leads to a further increase of expression. The stimulation by nitrate also occurs at the AOX protein and respiratory levels. A deletion analysis of the Aox1 promoter region demonstrates that a short upstream segment (-253 to +59 with respect to the transcription start site) is sufficient to ensure gene expression and regulation, but that distal elements are required for full gene expression. The observed pattern of AOX regulation points to the possible interaction between chloroplast and mitochondria in relation to a potential increase of photogenerated ATP when nitrate is used as a nitrogen source. [less ▲]

Detailed reference viewed: 11 (2 ULg)
Full Text
Peer Reviewed
See detailRegulation Of The Beta-Lactamase Blal Of Streptomyces-Cacaoi - The Product Of The Blab Regulatory Gene Is An Internal Membrane-Bound Protein
Magdalena, J.; Joris, Bernard ULg; Vanbeeumen, J. et al

in Biochemical Journal (1995), 311

The beta-lactamase-encoding gene blaL, cloned from Streptomyces cacaoi in Streptomyces lividans, is inducible by beta-lactam compounds. This regulation has been shown to depend on the products of two open ... [more ▼]

The beta-lactamase-encoding gene blaL, cloned from Streptomyces cacaoi in Streptomyces lividans, is inducible by beta-lactam compounds. This regulation has been shown to depend on the products of two open reading frames, ORF1 (blaA) and ORF2 (blaB) [Lenzini, Magdalena, Fraipont, Joris, Matagne and Dusart (1992) Mol. Gen. Genet. 235, 41-48]. BlaA belongs to the LysR family of transcription activators, whereas BlaB shares some features with the penicillin-recognizing proteins. BlaB has now been overexpressed in Escherichia coli, purified and used for antibody preparation. Immunoblotting of cell-fractionated materials from S. cacaoi showed that BlaB is attached to the internal face of the cytoplasmic membrane. It could not be released by high salt concentrations or EDTA, but only by protease treatment. Under the assay conditions, BlaB did not act as a penicillin-binding protein, a beta-lactamase, a D-amino-peptidase or a target in a phosphorylation step. [less ▲]

Detailed reference viewed: 9 (1 ULg)
Peer Reviewed
See detailRegulation of the chondrocyte metabolism by a new divalent strontium salt (S12911)
Henrotin, Yves ULg; Labasse, A; Deloffre, P et al

in BONE (1998), 23(S5), 344

Detailed reference viewed: 4 (0 ULg)
Peer Reviewed
See detailRegulation of the genes for ACTH and growth hormone by glucocorticoid hormones
Johnson, L. K.; Eberhardt, N. L.; Spindler, S. S. et al

in Endocrinology 1980 (1980)

Detailed reference viewed: 6 (1 ULg)
Full Text
See detailRegulation of the Immune Response by Innate Lymphocyte and Dendritic Cell Cross Talk
Reschner, Anca ULg; Langers, Inge ULg; Renoux, Virginie ULg et al

in Welles, Lorraine (Ed.) Dendritic Cells: Types, Life Cycles and Biological Functions (2010)

Dendritic cell (DC) is the generic name of different professional antigen presenting cell sub-populations, which are responsible for the initiation of specific immune responses. Recently, DC have been ... [more ▼]

Dendritic cell (DC) is the generic name of different professional antigen presenting cell sub-populations, which are responsible for the initiation of specific immune responses. Recently, DC have been involved in supporting innate immunity by interacting with various innate lymphocytes, such as natural killer (NK), NKT or γδ T (T cells expressing γδ T cell receptor). The functional links between innate lymphocytes and DC have been investigated widely and different studies demonstrated that the cross-talk between innate lymphocytes and DC was found to be multi-directional, involving not only cell-cell contacts but also soluble factors which lead to lymphocyte activation and DC maturation. The final outcome of these cellular interactions may have a dramatic impact on the quality and strength of the down-stream immune responses, mainly in the context of early responses to tumor cells and infectious agents. Interestingly, DC, NK and γδ T cells also share similar functions, such as antigen uptake and presentation, as well as cytotoxic and tumoricidal activity. In addition, NK and NKT cells have the ability to kill DC. This chapter will focus upon the different aspects of the cross-talk between DC and innate lymphocytes and its key role in all the steps of the immune response. These cellular interactions may be particularly critical in situations where immune surveillance requires efficient early innate responses. [less ▲]

Detailed reference viewed: 74 (26 ULg)
Full Text
Peer Reviewed
See detailRegulation of the mammalian heart function by nitric oxide.
MASSION, Paul ULg; Pelat, Michel; Belge, C. et al

in Comparative Biochemistry & Physiology Part A : Molecular & Integrative Physiology (2005), 142(2), 144-50

The mammalian heart expresses all three isoforms of nitric oxide synthases (NOS) in diverse cell types of the myocardium. Despite their apparent promiscuity, the NOS isoforms support specific signaling ... [more ▼]

The mammalian heart expresses all three isoforms of nitric oxide synthases (NOS) in diverse cell types of the myocardium. Despite their apparent promiscuity, the NOS isoforms support specific signaling because of their subcellular compartmentation with colocalized effectors and limited diffusibility of NO in muscle cells. eNOS and nNOS sustain normal EC coupling and contribute to the early and late phases of the Frank-Starling mechanism of the heart. They also attenuate the beta1-/beta2-adrenergic increase in inotropy and chronotropy, and reinforce the pre- and post-synaptic vagal control of cardiac contraction. By doing so, the NOS protect the heart against excessive stimulation by catecholamines, just as an "endogenous beta-blocker". In the ischemic and failing myocardium, induced iNOS further reinforces this effect, as does eNOS coupled to overexpressed beta3-adrenoceptors. nNOS expression also increases in the aging and infarcted heart, but its role (compensatory or deleterious) is less clear. In addition to their direct regulation of contractility, the NOS modulate oxygen consumption, substrate utilization, sensitivity to apoptosis, hypertrophy and regenerative potential, all of which illustrate the pleiotropic effects of this radical on the cardiac cell biology. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailRegulation of the mammalian heart function by nitric oxide.
MASSION, Paul ULg; Pelat, Michel; Belge, C. et al

in Comparative Biochemistry & Physiology Part A : Molecular & Integrative Physiology (2005), 142(2), 144-50

The mammalian heart expresses all three isoforms of nitric oxide synthases (NOS) in diverse cell types of the myocardium. Despite their apparent promiscuity, the NOS isoforms support specific signaling ... [more ▼]

The mammalian heart expresses all three isoforms of nitric oxide synthases (NOS) in diverse cell types of the myocardium. Despite their apparent promiscuity, the NOS isoforms support specific signaling because of their subcellular compartmentation with colocalized effectors and limited diffusibility of NO in muscle cells. eNOS and nNOS sustain normal EC coupling and contribute to the early and late phases of the Frank-Starling mechanism of the heart. They also attenuate the beta1-/beta2-adrenergic increase in inotropy and chronotropy, and reinforce the pre- and post-synaptic vagal control of cardiac contraction. By doing so, the NOS protect the heart against excessive stimulation by catecholamines, just as an "endogenous beta-blocker". In the ischemic and failing myocardium, induced iNOS further reinforces this effect, as does eNOS coupled to overexpressed beta3-adrenoceptors. nNOS expression also increases in the aging and infarcted heart, but its role (compensatory or deleterious) is less clear. In addition to their direct regulation of contractility, the NOS modulate oxygen consumption, substrate utilization, sensitivity to apoptosis, hypertrophy and regenerative potential, all of which illustrate the pleiotropic effects of this radical on the cardiac cell biology. [less ▲]

Detailed reference viewed: 2 (1 ULg)
Full Text
Peer Reviewed
See detailRegulation of the pigment optical density of an algal cell: filling the gap between photosynthetic productivity in the laboratory and in mass culture
Formighieri, Cinzia; Franck, Fabrice ULg; Bassi, Roberto

in Journal of Biotechnology (2012), 162

An increasing number of investors is looking at algae as a viable source of biofuels, beside Q3 cultivation for human/animal feeding or to extract high-value chemicals and pharmaceuticals. However ... [more ▼]

An increasing number of investors is looking at algae as a viable source of biofuels, beside Q3 cultivation for human/animal feeding or to extract high-value chemicals and pharmaceuticals. However, present biomass productivities are far below theoretical estimations implying that a large part of the available photosynthetically active radiation is not used in photosynthesis. Light utilisation inefficiency and rapid light attenuation within a mass culture due to high pigment optical density of wild type strains have been proposed as major limiting factors reducing solar-to-biomass conversion efficiency. Analysis of growth yields of mutants with reduced light-harvesting antennae and/or reduced overall pigment concentration per cell, generated by either mutagenesis or genetic engineering, could help understanding limiting factors for biomass accumulation in photobioreactor. Meanwhile, studies on photo-acclimation can provide additional information on the average status of algal cells in a photobioreactor to be used in modellingbased predictions. Identifying limiting factors in solar-to-biomass conversion efficiency is the first step for planning strategies of genetic improvement and domestication of algae to finally fill the gap between theoretical and industrial photosynthetic productivity. [less ▲]

Detailed reference viewed: 36 (10 ULg)
See detailRegulation of the xylanolytic system in Streptomyces species
Rigali, Sébastien ULg

Scientific conference (2003)

Detailed reference viewed: 3 (3 ULg)
See detailRegulation of training system for adults in educational technology
Denis, Brigitte ULg; Piette, Sylvie-Anne ULg

in Davies, Gordon; Stacey, Elisabeth (Eds.) Quality education @ a distance (2003)

This chapter reports the characteristics of a post graduate diploma on educational technologies based on a combination of face-to-face and distance activities and assesses its quality. We first define the ... [more ▼]

This chapter reports the characteristics of a post graduate diploma on educational technologies based on a combination of face-to-face and distance activities and assesses its quality. We first define the bases of the training program and present several tools (logbook, portfolio and questionnaires) which have been used to evaluate and regulate both training and individual learning process. Our observations indicate an increase and better regulation of the quality of the educational and learning processes. On the basis of the results we achieved, we propose that our original design could be extended to similar adult training programs. The way this innovative system is integrated in the universities shows an evolution due to its success that implies a certain kind of return on investment due to the demand of such kinds of device. [less ▲]

Detailed reference viewed: 17 (2 ULg)
Full Text
Peer Reviewed
See detailRegulation of training system for adults in educational technology
Denis, Brigitte ULg; Piette, Sylvie-Anne

in Stacey, E.; Davies, E. (Eds.) Quality education @ a distance (2003, February)

This paper reports the characteristics of a post graduate diploma on educational technologies based on a combination of face-to-face and distance activities and assesses its quality. We first define the ... [more ▼]

This paper reports the characteristics of a post graduate diploma on educational technologies based on a combination of face-to-face and distance activities and assesses its quality. We first define the bases of the training program and present several tools (logbook, portfolio and questionnaires) which have been used to evaluate and regulate both training and individual learning process. Our observations indicate an increase and better regulation of the quality of the educational and learning processes. On the basis of the results we achieved, we propose that our original design could be extended to similar adult training programs. The way this innovative system is integrated in the universities shows an evolution due to its success that implies a certain kind of return on investment due to the demand of such kinds of device. [less ▲]

Detailed reference viewed: 12 (2 ULg)