Synthesis of growth hormone by bacteria

in Nature (1978), 276(5690), 795-8

A hybrid gene was constructed between the beta-lactamase gene of plasmid pBR322 and the cloned coding sequence for rat growth hormone. This gene is expressed in bacteria and growth hormone sequences are ... [more ▼]

A hybrid gene was constructed between the beta-lactamase gene of plasmid pBR322 and the cloned coding sequence for rat growth hormone. This gene is expressed in bacteria and growth hormone sequences are detectable by immunological methods. [less ▲]

Synthesis of high-relaxivity contrast agents for magnetic resonance imaging by "click" chemistry

Poster (2007, August 31)

Synthesis of highly functionalized poly(alkyl cyanoacrylate) nanoparticles by means of click chemistry

in Macromolecules (2008), 41(22), 8418-8428

A general methodology was proposed to prepare highly functionalized poly(alkyl cyanoacrylate) nanoparticles by means of Huisgen 1,3-dipolar cyclo-addition, the so-called click chemistry. To achieve this ... [more ▼]

A general methodology was proposed to prepare highly functionalized poly(alkyl cyanoacrylate) nanoparticles by means of Huisgen 1,3-dipolar cyclo-addition, the so-called click chemistry. To achieve this goal, different protocols were investigated to obtain azidopoly(ethylene glycol) cyanoacetate of variable molar mass, followed by a Knoevenagel condensation−Michael addition reaction with hexadecyl cyanoacetate to produce a poly[(hexadecyl cyanoacrylate)-co-azidopoly(ethylene glycol) cyanoacrylate] (P(HDCA-co-N3PEGCA)) copolymer, displaying azide functionalities at the extremity of the PEG chains. As a proof of concept, model alkynes were quantitatively coupled either to the P(HDCA-co-N3PEGCA) copolymers in homogeneous medium followed by self-assembly in aqueous solution or directly at the surface of the preformed P(HDCA-co-N3PEGCA) nanoparticles in aqueous dispersed medium, both yielding highly functionalized nanoparticles. This versatile approach, using alkyl cyanoacrylate derivatives, opened the door to ligand-functionalized and biodegradable nanoparticles with “stealth” properties for biomedical applications. [less ▲]

Synthesis of highly ordered mesoporous compounds with control of morphology using a non-ionic surfactant as template

in Studies in Surface Science and Catalysis (2002), 141

Highly ordered hexagonal mesostructures (CMI-1 compounds) can be obtained under mild acidic conditions by working at low concentrations of non-ionic decaoxyethylene cetyl ether [C16(EO)10]. The present ... [more ▼]

Highly ordered hexagonal mesostructures (CMI-1 compounds) can be obtained under mild acidic conditions by working at low concentrations of non-ionic decaoxyethylene cetyl ether [C16(EO)10]. The present work shows that it is possible to gain control at the nanometer scale over the packing symmetry of the channels, as well as at the micrometer level over the morphology of the particles by varying the surfactant / silica molar ratio and the hydrothermal treatment conditions. Very high loadings of silica precursors typically afford highly ordered hexagonal CMI-1 compounds whereas an increase of the surfactant / silica molar ratio results in materials with a more disordered channel array. In a parallel way, very low molar ratios of surfactant / silica lead to ropes, gyroids and toroids whereas spheres are the most stable shape with the lower quantities of silica. From this point, it appears thus that not only the structure but also the morphologies encountered for MCM-41 type mesoporous silica can be reproduced with a non-ionic templating agent. [less ▲]

Synthesis of large pore disordered MSU-type mesoporous silicas through the assembly of C16(EO)10 surfactant and TMOS silica source: Effect of the hydrothermal treatment and thermal stability of materials

in Journal of Physical Chemistry B (2001), 105(26), 6070-6079

Decaoxyethylene cetyl ether [C 16(EO) 10] has been employed as templating agent for large pore disordered MSU-type mesoporous silicas synthesis. The effect of the hydrothermal treatment (heating time and ... [more ▼]

Decaoxyethylene cetyl ether [C 16(EO) 10] has been employed as templating agent for large pore disordered MSU-type mesoporous silicas synthesis. The effect of the hydrothermal treatment (heating time and temperature) on the textural properties, in particular, pore diameter of materials has been investigated. The obtained materials have been characterized by different techniques such as X-ray diffraction, scanning and transmission electron microscopy and nitrogen adsorption-desorption analysis. The thermal stability of the compounds is also evaluated. Our results show that the pore diameter depends strongly on the heating time and temperature. This is the result of a competition between the stretching of the surfactant chains, which can lead to the breakdown of the walls separating adjacent pores, and the reorganization of micelles with heating time and temperature. At higher temperature or for longer durations, a more extended surfactant molecular conformation can be obtained, which leads to materials with larger pore sizes. A reorganization of the micellar solution during the hydrothermal treatment in autoclave can also simultaneously occur to give materials with smaller pore diameter. It is found that compounds prepared with the nonionic surfactant exhibit an enhanced thermal stability compared to those synthesized with cetyltrimethylammonium bromide (CTMABr) as templating agent. This higher stability is related to the synthesis pathway and the structure of materials. [less ▲]

Synthesis of light-responsive poly(vinyl alcohol)-C60 nanohybrids for cancer photodynamic therapy

Poster (2008, October 28)

Synthesis of macrocyclic polyesters

Poster (2003, May 16)

Synthesis of microsphere-loaded porous polymers by combining emulsion and dispersion polymerisations in supercritical carbon dioxide

in Chemical Communications (2012), 48(67), 8356-8358

Highly porous materials were produced by acrylamide polymerisation templated by supercritical CO2-in-water emulsions using new fluorinated glycosurfactants. Properties of the resulting polymer scaffolds ... [more ▼]

Highly porous materials were produced by acrylamide polymerisation templated by supercritical CO2-in-water emulsions using new fluorinated glycosurfactants. Properties of the resulting polymer scaffolds were tuned by performing dispersion polymerisations within their cavities filled with supercritical CO2. [less ▲]

The Synthesis of N-Benzyl-2-azanorbornene via Aqueous Hetero Diels-Alder Reaction

in Journal of Chemical Education (2008), 85(11), 1538-1540

The synthesis of N-benzyl-2-azanorbornene via aqueous hetero Diels-Alder reaction of cyclopentadiene and benzyliminium chloride formed in situ from benzylamine hydrochloride and formaldehyde is described ... [more ▼]

The synthesis of N-benzyl-2-azanorbornene via aqueous hetero Diels-Alder reaction of cyclopentadiene and benzyliminium chloride formed in situ from benzylamine hydrochloride and formaldehyde is described. Characterization of the product was achieved by IR and NMR spectroscopies. The spectral data acquired are thoroughly discussed. Numerous coupling constants were extracted from the 1H NMR spectrum. They provide a valuable material to familiarize students with the different types of proton-proton coupling patterns and their typical ranges. Karplus type correlations served to calculate 3J vicinal coupling constants as a function of dihedral angles. These computational studies involved molecular modeling. Because the synthetic part is rather straightforward and easy to carry out, this experiment is suitable for an introductory laboratory course where it can serve to illustrate the concepts of green chemistry and atom efficiency. Emphasis can also be placed on structural analysis for use within a spectroscopy course even if there is no activity component associated with it. Last but not least, the whole project provides ample materials for an advanced undergraduate laboratory in green chemistry that combines organic synthesis, structural analysis, and theoretical calculations. [less ▲]

Synthesis of Na2FePO4F/Carbon composite cathode materials for lithium-ion batteries

Conference (2013, January 30)