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See detailLes pure players, une alternative journalistique
Colmant, François ULg

Scientific conference (2012, May 23)

Alors que la presse écrite traditionnelle francophone affronte depuis des années une sévère crise endémique et systémique, une alternative journalistique est apparue avec l’arrivée des pure players. Ces ... [more ▼]

Alors que la presse écrite traditionnelle francophone affronte depuis des années une sévère crise endémique et systémique, une alternative journalistique est apparue avec l’arrivée des pure players. Ces acteurs médiatiques, uniquement présents en ligne, dessinent les contours d’une autre presse et tendent à définir un nouveau modèle d’information de masse. Actifs depuis quelques années, ces sites, calqués à l’origine sur le modèle du Huffington Post (premier du genre), occupent désormais une réelle place médiatique et cultivent une approche de l’information et du journalisme volontairement différente des médias classiques, toujours adossés à un support papier. Aux prémices de la transition vers le paper free, cette nouvelle presse entend prendre à contre-courant l’ambition annoncée par plusieurs grands groupes de presse de modifier profondément la façon d’informer, où l’immédiateté serait érigée en règle d’or. Angles différents, actualités alternatives et investigation prononcée deviennent les maîtres mots d’un média essentiellement animé par d’historiques figures de la presse classique, associés à de jeunes journalistes pratiquement « nés » sur Internet. Gratuits ou payants, ces nouveaux médias, qui privilégient un autre rapport au lecteur et à l’information, gagnent petit à petit en reconnaissance mais peinent encore à se faire considérer comme l’égal de leurs cousins de papier. Malgré leur sérieux et leur rigueur, il semble que le support numérique, malgré son accessibilité accrue, souffre encore d’un profond déficit de légitimité auprès des caciques de la profession. Devant cet autre possible journalistique, notre approche consistera à interroger cette nouvelle donne, étudier son modèle économique – sensiblement différent – et questionner les changements concrets que cette approche induit sur le comportement et les conditions de travail, au quotidien, des journalistes. [less ▲]

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See detailPure red cell aplasia following peripheral stem cell transplantation: complete response to a short course of high-dose recombinant human erythropoietin.
Martelli, M.; Ponchio, L.; Beguin, Yves ULg et al

in Haematologica (1994), 79(5), 456-9

We studied a patient who developed pure red cell aplasia (PRCA) following peripheral stem cell transplantation for non-Hodgkin lymphoma. Serum erythropoietin was appropriate for the degree of anemia ... [more ▼]

We studied a patient who developed pure red cell aplasia (PRCA) following peripheral stem cell transplantation for non-Hodgkin lymphoma. Serum erythropoietin was appropriate for the degree of anemia. Corticosteroid treatment was ineffective. Four months after transplantation rHuEpo was administered subcutaneously at a dose of 150 U/Kg per day, five days a week for 8 weeks. Treatment induced an erythropoietic response and corrected anemia. Response was maintained following discontinuation of rHuEpo. This study and previous reports indicate that high doses of rHuEpo given over a short time can resolve PRCA following autologous or allogeneic stem cell transplantation. [less ▲]

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See detailPure species of grass discrimination with the help of hyperspectral imaging NIR
Dale, Laura ULg; Rotar, Ioan; Bogdan, Anca Dorina et al

in Agronomy Series of Scientific Research = Lucrari Stiintifice Seria Agronomie (2011, December), 54(2), 23-27

The possibilities to discriminate with hyperspectral imaging system, SWIR ImSpector N25E in grass mixtures of the pure species Festuca rubra L., Trifolium repens L., Agrostis capillaris L., Hieracium ... [more ▼]

The possibilities to discriminate with hyperspectral imaging system, SWIR ImSpector N25E in grass mixtures of the pure species Festuca rubra L., Trifolium repens L., Agrostis capillaris L., Hieracium aurantiacum L., Arnica montana L.was the objective of this study. All the samples were collected from natural meadows, from the National Apuseni Park, Apuseni Mountains, from Gârda area. The samples were naturally dried then prepared using the protocol for NIRS analyze. The model built with PLS–DA, was used to demonstrate wether the classes discrimination between pure species is possible or not. The goal of this study was to see in other images if the pure species are or are not recognized according to the spectral data base. The potential of using the Hyperspectral Imaging NIR (Camera NIR) to discriminate or to identify pure species was confirmed. For this technique the MatLab program was used .A percentage of more than 96% correct prediction for species discrimination was obtained. This study should drive to a more important point, which is to verify whether the toxic species are or are not used as food for animals in the natural meadows. The floristic composition of a meadow can be determined only if we have in the data base, dates for each identified species, as being part of the mix. [less ▲]

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See detailA purely distributed implementation of undervoltage load shedding
Otomega, Bogdan; Glavic, Mevludin; Van Cutsem, Thierry ULg

(2007, June)

A new design of load shedding against long-term voltage instability is proposed. It uses a set of distributed controllers, each monitoring a transmission voltage and controlling a group of loads. Each ... [more ▼]

A new design of load shedding against long-term voltage instability is proposed. It uses a set of distributed controllers, each monitoring a transmission voltage and controlling a group of loads. Each controller acts in closed-loop, shedding amounts that vary in magnitude and time according to the evolution of its monitored voltage. The whole system operates without information exchange between controllers, the latter being implicitly coordinated through network voltages. The operation, design and robustness of the proposed scheme are illustrated through a small but realistic example. [less ▲]

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See detailPurely Flavored Leptogenesis
Aristizabal Sierra, Diego ULg; Munoz, Luis Alfredo; Enrico, Nardi

in Physical Review D (2009), 80

We study a model for leptogenesis in which the total CP asymmetries in the decays and scatterings involving the SU(2) singlet seesaw neutrinos Nα vanish (ϵNα=0). Leptogenesis is possible due to ... [more ▼]

We study a model for leptogenesis in which the total CP asymmetries in the decays and scatterings involving the SU(2) singlet seesaw neutrinos Nα vanish (ϵNα=0). Leptogenesis is possible due to nonvanishing CP violating lepton flavor asymmetries, realizing a situation in which the baryon asymmetry is due exclusively to flavor effects. We study the production of a net lepton asymmetry by solving the Boltzmann equations specific to this model, and we show that successful leptogenesis can be obtained at a scale as low as the TeV. We also discuss constraints on the model parameter space arising from current experimental upper limits on lepton flavor violating decays. [less ▲]

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See detailPurificacao, caracterizacao e dosagem radioimunologica de glicoproteinas associadas a gestacao em zebuinos
Melo de Sousa, Noelita ULg

Doctoral thesis (2002)

The pregnancy-associated glycoproteins (PAGs) constitute a large family of aspartic proteinases expressed in the outer epithelial cell layer of the Artyodactyla placenta. In the first part of the present ... [more ▼]

The pregnancy-associated glycoproteins (PAGs) constitute a large family of aspartic proteinases expressed in the outer epithelial cell layer of the Artyodactyla placenta. In the first part of the present work, two biochemical approaches were used to characterize PAGs isolated from zebu (Bos indicus) fetal cotyledons. The first procedure, used to isolate PAG from zebu placenta removed late in pregnancy, included extraction of proteins at neutral pH, acidic and ammonium sulfate precipitations, anion and cation exchange chromatographies. The second procedure, used to investigate PAG in placentas removed at early and mid gestational periods, included protein extractions at acid or alkaline pH followed by pepstatin-A agarose affinity chromatography. A bovine PAG radioimmunoassay was used to monitor the immunoreactivity throughout the isolation procedures. The most immunoreactive fractions issued from cation exchange and affinity chromatographies were analyzed by SDS-PAGE and Western Blot, before transfer to polyvinylidene difluoride (PVDF) membrane for NH2-microsequence determination. By use of SDS-PAGE and Western Blot, different isoforms of PAG with apparent molecular masses from 51 to 69 kDa and isoeletric points varying from 3.1 to 6.7 were identified in placentas from different gestational ages. After CM ceramic chromatography of all except 0.32 M NaCl DEAE fraction, the most immunoreactive proteins revealed N-terminal amino acid sequences (10 to 25 aa long) which were 100% identical to bovine PAG-1. The same sequence (14 aa long) was found after pepstatin-agarose affinity chromatography of proteins extracted from placentas removed earlier in pregnancy. These results converged towards the expression of one major N-terminal PAG amino acid sequence in zebu placentas at different gestational ages. In the second part of this study, two specific RIA systems were developed then used to measure plasmatic PAG concentrations during gestation and postpartum period in Azawak zebu cows. Twelve females palpated per rectum and diagnosed as pregnant were bled at 5-10 days interval approximately from Week 8 of gestation till Week 10 postpartum (pp). One zebu cow initially diagnosed as pregnant showed PAG concentrations lower than the assay sensitivity (<0.20 ng/ml) and did not calve. Another cow showed abnormally high PAG concentrations during gestation, being excluded from the general PAG profile. The 10 other zebu cows gave a very homogeneous PAG profile. In these animals, concentrations increased progressively from Week 8 to Week 35 of gestation (from 6.04.2 to 196.034.8 ng/ml), remaining relatively constant until Week 39 (210.874.8 ng/ml), when they increased sharply to reach their highest level (1,095.6607.2 ng/ml) at parturition. After delivery, PAG concentrations declined significantly (P<0.05) till the Week 2 postpartum (348.4  85.6 ng/ml) and slowly till the Week 10 postpartum. These results revealed that PAG concentrations in zebu cattle were lower than those previously described in taurine breeds between week 35 and 39 of gestation. [less ▲]

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See detailPurification and analysis by mass spectometry of low M.W. soluble factors released into conditioned culture media by B16 melanoma cells
Gillet, Marie-Claire ULg; Siwek, Brigitte; Quetin-Leclercq, Joelle et al

Poster (1995, July)

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See detailPurification and Biochemical Characterization of Recombinant Human Placental Growth Hormone Produced in Escherichia Coli
Igout, Ahmed ULg; Van Beeumen, Jozef; Frankenne, Francis ULg et al

in Biochemical Journal (1993), 295(3), 719-724

The hGH-V (or hGH-2) gene codes for human placental growth hormone (hPGH). Secretion of hPGH is continuous, in contrast with the pulsed secretion of pituitary growth hormone (hGH) which it progressively ... [more ▼]

The hGH-V (or hGH-2) gene codes for human placental growth hormone (hPGH). Secretion of hPGH is continuous, in contrast with the pulsed secretion of pituitary growth hormone (hGH) which it progressively replaces in the maternal bloodstream. hGH- V cDNA has previously been cloned and isolated. Analysis of its nucleotide sequence has revealed a 191-residue protein, hPGH, differing from hGH at 13 positions. The calculated pI is more basic than that of the pituitary hormone. Here we have inserted hGH- VcDNA into the pIN-III-ompA3 plasmid in order to produce hPGH in its native form in Escherichia coli D1210. Expression of hGH- V cDNA in E. coli is significantly lower than that of hGH cDNA with the same expression system. The hPGH produced in E. coli was purified in quantities sufficient to allow its biochemical and immunochemical characterization. The molecular mass of the protein was determined by electrospray m.s. The determined mass, 22320 Da, agrees well with the molecular mass calculated from the translated cDNA sequence, assuming the presence of two disulphide bridges. Having established the technique for producing hPGH with a primary structure identical to the natural, non-glycosylated, 22 kDa isoform, we can now plan the full physicochemical and pharmaceutical characterization of this new hormonal entity. [less ▲]

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See detailPurification and characterisation of a 31 kDa chitinase from the Myzus persicae aphid, a target for Hemiptera biocontrol
Francis, Frédéric ULg; Saguez, Julien; Cherqui, Anas et al

in Applied Biochemistry and Biotechnology (2012), 166

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See detailPurification and characterisation of phage tail-like bacteriocin induced in Serratia sp.
Jabrane, A.; Vandenberghe, I.; Van beeumen, J. et al

Poster (1997, August)

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See detailPurification and Characterization of a 315 Kda Keratinolytic Subtilisin-Like Serine Protease from Microsporum Canis and Evidence of Its Secretion in Naturally Infected Cats
Mignon, Bernard ULg; Swinnen, M.; Bouchara, J. P. et al

in Medical Mycology (1998), 36(6), 395-404

A keratinolytic protease, secreted as the major component by a feline clinical isolate of Microsporum canis cultivated in a minimal medium containing cat keratin, was purified by affinity chromatography ... [more ▼]

A keratinolytic protease, secreted as the major component by a feline clinical isolate of Microsporum canis cultivated in a minimal medium containing cat keratin, was purified by affinity chromatography on bacitracin agarose and gel filtration. The apparent molecular mass of the enzyme was 31.5 kDa and the pI was 11.8. The enzyme was not glycosylated and its first 15 N-terminal amino acids showed numerous similarities with other fungal subtilisins. The optimum pH was around 9 while inactivation of the enzyme was reversible at pH 4, but not at pH 11. The enzyme was stable at 37 degrees C with an apparent optimum temperature around 55 degrees C. PMSF, soybean trypsin inhibitor (SBTI) and chymostatin strongly inhibited the proteinase. The highest affinity (Km of 0.37 mM) and physiological efficiency (k(cat)/Km) were obtained for the synthetic substrate N-Suc-Ala-Ala-Pro-Phe-p-nitroanilide. These results indicate that the keratinase belongs to the subtilisin-like serine protease family. Purified rabbit immunoglobulins G prepared against the keratinase and used in an immunohistochemical test allowed the detection of the keratinase produced by the fungus invading hair structures in naturally infected cats. The in vitro keratinolytic activity of the enzyme and its production in vivo suggest that it may contribute to pathogenicity. [less ▲]

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See detailPurification and characterization of a 43.5 kDa keratinase from Microsporum canis
Brouta, F.; Descamps, F.; Fett, Thomas ULg et al

Conference (2000)

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See detailPurification and characterization of a 43.5 kDa keratinolytic metalloprotease from Microsporum canis.
Brouta, F.; Descamps, F.; Fett, Thomas ULg et al

in Medical Mycology (2001), 39(3), 269-275

A keratinolytic protease secreted by a feline clinical isolate of Microsporum canis cultivated in a broth containing feline keratin as the sole nitrogen source was purified from the culture filtrate by ... [more ▼]

A keratinolytic protease secreted by a feline clinical isolate of Microsporum canis cultivated in a broth containing feline keratin as the sole nitrogen source was purified from the culture filtrate by affinity chromatography on bacitracin-agarose and by hydrophobic chromatography on octyl-agarose. The enzyme had an apparent molecular mass of 43.5 kDa and the pI was 7.7. It had a significant activity against keratin azure, elastin-Congo red and denatured type I collagen (azocoll). Using the latter substrate, the optimum pH was around 8 and the apparent optimum temperature around 50 degrees C. The protease was strongly inhibited by 1,10-phenanthroline, phosphoramidon and EDTA. The first 13 N-terminal amino acid sequence showed a 61% homology with that of the extracellular metalloprotease of Aspergillus fumigatus and with the neutral protease I of A. oryzae, confirming that this 43.5 kDa keratinase is a metalloprotease. This keratinolytic metalloprotease could be a virulence-related factor involved in pathophysiological mechanisms of M. canis dermatophytosis. [less ▲]

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See detailPurification and characterization of a bovine pregnancy-associated glycoprotein
Zoli, André Pagnah; Beckers, Jean-François ULg; Wouters-Ballman, Patricia et al

in Biology of Reproduction (1991), 45(1), 1-10

A 67000 Mr bovine pregnancy-associated glycoprotein (bPAG) has been isolated from fetal cotyledons and purified to homogeneity by HPLC. The purification was monitored by a double immunodiffusion test and ... [more ▼]

A 67000 Mr bovine pregnancy-associated glycoprotein (bPAG) has been isolated from fetal cotyledons and purified to homogeneity by HPLC. The purification was monitored by a double immunodiffusion test and by RIA in conjunction with an antiserum raised against a crude fraction of placenta-specific antigens. The molecular weight of bPAG was estimated to be 67000 by SDS-PAGE. The isoelectric points (pI) of the four isoforms, determined by high-resolution analytical electrofocusing in polyacrylamide gel, were 4.4, 4.6, 5.2, and 5.4. The carbohydrate content of the bPAG consisted of approximately 10.02 +/- 1.09% neutral sugar and variant amounts of sialic acid (from 0.29 +/- 0.06% in the most basic isoform to 2.1 +/- 0.31% in the most acidic isoform). A specific antiserum was raised against the purified bPAG. A specific RIA showed that the bPAG was antigenically unrelated to BSA, alphafetoprotein (AFP), and human schwangerschafts-spezifischen (pregnancy-specific) beta 1 glycoprotein (SP1). According to some characteristics (e.g. the molecular weight), the purified bPAG may correspond to a form of the pregnancy-specific protein B previously described by Sasser and colleagues (Biol Reprod 1986; 35:936-942). [less ▲]

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See detailPurification and characterization of a carboxylesterase involved in malathion-specific resistance from Tribolium castaneum (Coleoptera: Tenebrionidae).
Amichot, Marcel; Haubruge, Eric ULg; Bergé, Jean-Baptiste et al

in Insect Biochemistry and Molecular Biology (2008), 32

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See detailPurification and characterization of a microbial dehydrogenase - A vanillin : NAD(P)(+) oxidoreductase
Bare, G.; Swiatkowski, T.; Moukil, A. et al

in Applied Biochemistry and Biotechnology (2002), 98-100(Spring), 415-428

Pseudomonas fluorescens (strain BTP9) was found to have at least two NAD(P)-dependent vanillin dehydrogenases: one is induced by vanillin, and the other is constitutive. The constitutive enzyme was ... [more ▼]

Pseudomonas fluorescens (strain BTP9) was found to have at least two NAD(P)-dependent vanillin dehydrogenases: one is induced by vanillin, and the other is constitutive. The constitutive enzyme was purified by ammonium sulfate fractionation, gel-filtration, and Q-Sepharose chromatography. The subunit Mr value was 55,000, determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The native M, value estimated by gel-filtration chromatography gave a value of 210,000. The enzyme made use of NAD(+) less effectively than NADP(+). Benzaldehyde, 4-hydroxybenzaldehyde, hexanal, and acetaldehyde were not oxidized at detectable rates in the presence of NAD(+) or NADP(+). The ultraviolet absorption spectrum indicated that there is no cofactor or prosthetic group bound. The vanillin oxidation reaction was essentially irreversible. The pH optimum was 9.5 and the pI of the enzyme was 4.9. Enzyme activity was not affected when assayed in the presence of salts, except FeCl2. The enzyme was inhibited by the thiol-blocking reagents 4-chloromercuribenzoate and N-ethylmaleimide. NAD(+) and NADP(+) protected the enzyme against such a type of inhibition along with vanillin to a lesser extent. The enzyme exhibited esterase activity with 4-nitrophenyl acetate as substrate and was activated by low concentrations of NAD(+) or NADP(+). We compared the properties of the enzyme with those of some well-characterized microbial benzaldehyde dehydrogenases. [less ▲]

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See detailPurification and characterization of a pregnancy-associated glycoprotein (ovPAG-6) from sheep placenta removed between 66 to 100 days of gestation.
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 12-13

Detailed reference viewed: 8 (0 ULg)