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See detailPsychrophilic enzymes: cool responses to chilly problems
Roulling, Frédéric ULg; Piette, Florence ULg; Cipolla, Alexandre ULg et al

in Horikoshi, K.; Antranikian, G.; Bull, A. (Eds.) et al Extremophiles Handbook (2011)

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See detailPsychrophilic enzymes: from folding to function and biotechnology
Feller, Georges ULg

in Scientifica (2013), 2013(Article ID 512840), 1-28

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See detailPsychrophilic enzymes: Hot topics in cold adaptation
Feller, Georges ULg; Gerday, Charles ULg

in Nature Reviews Microbiology (2003), 1(3), 200-208

More than three-quarters of the Earth's surface is occupied by cold ecosystems, including the ocean depths, and polar and alpine regions. These permanently cold environments have been successfully ... [more ▼]

More than three-quarters of the Earth's surface is occupied by cold ecosystems, including the ocean depths, and polar and alpine regions. These permanently cold environments have been successfully colonized by a class of extremophilic microorganisms that are known as psychrophiles (which literally means cold-loving). The ability to thrive at temperatures that are close to, or below, the freezing point of water requires a vast array of adaptations to maintain the metabolic rates and sustained growth compatible with life in these severe environmental conditions. [less ▲]

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See detailPsychrophilic Enzymes: Molecular Basis of Cold Adaptation
Feller, Georges ULg; Gerday, Charles ULg

in Cellular and Molecular Life Sciences : CMLS (1997), 53(10), 830-41

Psychrophilic organisms have successfully colonized polar and alpine regions and are able to grow efficiently at sub-zero temperatures. At the enzymatic level, such organisms have to cope with the ... [more ▼]

Psychrophilic organisms have successfully colonized polar and alpine regions and are able to grow efficiently at sub-zero temperatures. At the enzymatic level, such organisms have to cope with the reduction of chemical reaction rates induced by low temperatures in order to maintain adequate metabolic fluxes. Thermal compensation in cold-adapted enzymes is reached through improved turnover number and catalytic efficiency. This optimization of the catalytic parameters can originate from a highly flexible structure which provides enhanced abilities to undergo conformational changes during catalysis. Thermal instability of cold-adapted enzymes is therefore regarded as a consequence of their conformational flexibility. A survey of the psychrophilic enzymes studied so far reveals only minor alterations of the primary structure when compared to mesophilic or thermophilic homologues. However, all known structural factors and weak interactions involved in protein stability are either reduced in number or modified in order to increase their flexibility. [less ▲]

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See detailPsychrophilic Enzymes: Revisiting the Thermodynamic Parameters of Activation May Explain Local Flexibility
Lonhienne, T.; Gerday, Charles ULg; Feller, Georges ULg

in Biochimica et Biophysica Acta (2000), 1543(1), 1-10

Basic theoretical and practical aspects of activation parameters are briefly reviewed in the context of cold-adaptation. In order to reduce the error impact inherent to the transition state theory on the ... [more ▼]

Basic theoretical and practical aspects of activation parameters are briefly reviewed in the context of cold-adaptation. In order to reduce the error impact inherent to the transition state theory on the absolute values of the free energy (DeltaG(#)), enthalpy (DeltaH(#)) and entropy (DeltaS(#)) of activation, it is proposed to compare the variation of these parameters between psychrophilic and mesophilic enzymes, namely Delta(DeltaG(#))(p-m), Delta(DeltaH(#))(p-m) and Delta(DeltaS(#))(p-m). Calculation of these parameters from the available literature shows that the main adaptation of psychrophilic enzymes lies in a significant decrease of DeltaH(#), therefore leading to a higher k(cat), especially at low temperatures. Moreover, in all cases including cold-blooded animals, DeltaS(#) exerts an opposite and negative effect on the gain in k(cat). It is argued that the magnitude of this counter-effect of DeltaS(#) can be reduced by keeping some stable domains, while increasing the flexibility of the structures required to improve catalysis at low temperature, as demonstrated in several cold-active enzymes. This enthalpic-entropic balance provides a new approach explaining the two types of conformational stability detected by recent microcalorimetric experiments on psychrophilic enzymes. [less ▲]

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See detailPsychrophilic microorganisms: challenges for life
D'Amico, Salvino ULg; Collins, T.; Marx, J. C. et al

in EMBO Reports (2006), 7(4), 385-389

The ability of psychrophiles to survive and proliferate at low temperatures implies that they have overcome key barriers inherent to permanently cold environments. These challenges include: reduced enzyme ... [more ▼]

The ability of psychrophiles to survive and proliferate at low temperatures implies that they have overcome key barriers inherent to permanently cold environments. These challenges include: reduced enzyme activity; decreased membrane fluidity; altered transport of nutrients and waste products; decreased rates of transcription, translation and cell division; protein cold- denaturation; inappropriate protein folding; and intracellular ice formation. Cold- adapted organisms have successfully evolved features, genotypic and/ or phenotypic, to surmount the negative effects of low temperatures and to enable growth in these extreme environments. In this review, we discuss the current knowledge of these adaptations as gained from extensive biochemical and biophysical studies and also from genomics and proteomics. [less ▲]

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See detailPsychrophilic organisms and their enzymes as new tools in biotechnology
Gerday, Charles ULg; Chessa, Jean Pierre; Feller, Georges ULg

Conference (1999)

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See detailPt/C catalyst for PEM fuel cells: Control of Pt nanoparticles characteristics through a novel plasma deposition method
Laurent-Brocq, Mathilde; Job, Nathalie ULg; Eskenazi, David ULg et al

in Applied Catalysis B : Environmental (2014), 147

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See detailPt/C catalyst for PEM fuel cells: low pressure and low temperature plasma synthesis of Pt nanoparticles
Devant, Ludovic; Laurent-Brocq, Mathilde; Job, Nathalie ULg et al

Conference (2014)

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See detailPt/carbon xerogel catalysts for PEM fuel cells
Job, Nathalie ULg; Maillard, F.; Pirard, Jean-Paul ULg et al

in Proceedings of the Fundamentals & Developments of Fuel Cells (FDFC) 2011 Conference (2011, January 19)

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See detailPterostichus rhaeticus Heer 1837 : une nouvelle espèce pour le Grand-Duché de Luxembourg (Coleoptera : Carabidae).
Mercatoris, Nico; Desender, Konjev; Dufrêne, Marc ULg

in Paiperleck (1988), 10(1), 20-23

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See detailPTH : what are we really assaying ?
Cavalier, Etienne ULg

Conference (2005, November 25)

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See detailPTH élevée en ciel serein.
Cavalier, Etienne ULg

Conference given outside the academic context (2007)

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See detailPTH in Chronic Kidney Disease - What do the KDIGO Guidelines Change
CAVALIER, Etienne ULg

Conference (2012, June 14)

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See detailPTH in hemodialyzed patients
Cavalier, Etienne ULg

Conference (2007, May 24)

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See detailPTH oxidation after a session of hemodialyze: myth or reality?
Cavalier, Etienne ULg; Delanaye, Pierre ULg; Carlisi, Ignazia ULg et al

in Immuno-Analyse & Biologie Spécialisée [=IBS] (2006, October)

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See detailPTH stability : preanalytical conditios influences for Liaison (Diasorin)
Cavalier, Etienne ULg; Delanaye, Pierre ULg; Carlisi, Ignazia ULg et al

in Clinical Chemistry & Laboratory Medicine (2007, June), 45

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See detailPTH: que dosons-nous réellement?
Cavalier, Etienne ULg

Conference (2006, October 26)

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See detailPTV-LV Injection Coupled to Capillary GC Ion Trap MS/MS as Alternative Method for PCDD/Fs Analysis in Foods.
Eppe, Gauthier ULg; Focant, Jean-François ULg; Pirard, C. et al

in Organohalogen compounds (2001), 50

Detailed reference viewed: 31 (0 ULg)