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See detailNew approach for describing nuclear reactions based on Intra-Nuclear Cascade model with DWBA
Hashimoto, S.; Iwamoto, Y.; Sato, T. et al

in Progress in Nuclear Science and Technology (2014), 4

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See detailA New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Wel
Mille, Caroline; Debarnot, Dominique; Zorzi, Willy ULg et al

in Biosensors (2012), 2(4), 433-447

This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the ... [more ▼]

This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation. [less ▲]

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See detailA New Approach for the Characterization of Organic Residues from Stone Tools Using GC×GC-TOFMS
Perrault, Katelynn ULg; Stefanuto, Pierre-Hugues ULg; Dubois, Lena ULg et al

in Separations (2016), 3(2), 16

Headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) have traditionally been used, in combination with other analyses, for the chemical characterization of ... [more ▼]

Headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) have traditionally been used, in combination with other analyses, for the chemical characterization of organic residues recovered from archaeological specimens. Recently in many life science fields, comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS) has provided numerous benefits over GC-MS. This study represents the first use of HS-SPME-GC×GC-TOFMS to characterize specimens from an experimental modern reference collection. Solvent extractions and direct analyses were performed on materials such as ivory, bone, antlers, animal tissue, human tissue, sediment, and resin. Thicker film column sets were preferred due to reduced column overloading. The samples analyzed by HS-SPME directly on a specimen appeared to give unique signatures and generally produced a higher response than for the solvent-extracted residues. A non-destructive screening approach of specimens may, therefore, be possible. Resin and beeswax mixtures prepared by heating for different lengths of time appeared to provide distinctly different volatile signatures, suggesting that GC×GC-TOFMS may be capable of differentiating alterations to resin in future studies. Further development of GC×GC-TOFMS methods for archaeological applications will provide a valuable tool to uncover significant information on prehistoric technological changes and cultural behavior. [less ▲]

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See detailNew approach for the detection of growth promotors residues in animal products
Scippo, Marie-Louise ULg; Helbo, Vincent; Degand, Guy ULg et al

in Third meeting on animal productions. Biotechnologies: society stake. Proceedings of the study day held in Gembloux (Belgium) the 28 January 1998 (1998)

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See detailNew approach for the detection of non-ribosomal peptide synthetase genes in Bacillus strains by polymerase chain reaction.
Tapi, Arthur; Chollet-Imbert, Marlene; Scherens, Bart et al

in Applied microbiology and biotechnology (2010), 85(5), 1521-31

Bacillus strains produce non-ribosomal lipopeptides that can be grouped into three families: surfactins or lichenysins, iturins and fengycins or plispastatins. These biosurfactants show a broad spectrum ... [more ▼]

Bacillus strains produce non-ribosomal lipopeptides that can be grouped into three families: surfactins or lichenysins, iturins and fengycins or plispastatins. These biosurfactants show a broad spectrum of biological activities. To detect strains able to produce these lipopeptides, a new polymerase chain reaction screening approach was developed using degenerated primers based on the intraoperon alignment of adenylation and thiolation nucleic acid domains of all enzymes implicated in the biosynthesis of each lipopeptide family. The comparative bioinformatics analyses of each operon led to the design of four primer pairs for the three families taking into account the differences between open reading frames of each synthetase gene. Tested on different Bacillus sp. strains, this technique was used successfully to detect not only the expected genes in the lipopeptide producing strains but also the presence of a plispastatin gene in Bacillus subtilis ATCC 21332 and a gene showing a high similarity with the polyketide synthase type I gene in the B. subtilis ATCC 6633 genome. It also led to the discovery of the presence of non-ribosomal peptide synthetase genes in Bacillus thuringiensis serovar berliner 1915 and in Bacillus cereus LMG 2098. In addition, this work highlighted the differences between the fengycin and plipastatin operon at DNA level. [less ▲]

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See detailA new approach in deciphering early protist paleobiology and evolution: Combined microscopy and microchemistry of single Proterozoic acritarchs
Javaux, Emmanuelle ULg; Marshal, C. P.

in Review of Palaeobotany and Palynology (2006), 139(1-4), 1-15

Beside a few cases, the biological affinities of Proterozoic and Paleozoic acritarchs remain, by definition, largely unknown. However, these fossils record crucial steps in the early evolution of ... [more ▼]

Beside a few cases, the biological affinities of Proterozoic and Paleozoic acritarchs remain, by definition, largely unknown. However, these fossils record crucial steps in the early evolution of microorganisms and diversification of complex ecosystems. We present how combining microscopy (light microscopy, scanning and transmitted electron microscopy) with microchemical analyses of individual microfossils may offer further insights into the paleobiology and evolution of early microorganisms. We use our ongoing work on early Mesoproterozoic and Neoproterozoic assemblages, as well as other published work, as examples to illustrate how this approach may clarify the evolution of early microorganisms and we underline how useful this approach could be for palynologists working on younger material. Such a multidisciplinary approach offers new possibilities to investigate the biological affinities of acritarchs and the record of early life on Earth and beyond. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

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See detailNew approach in modelling floods over steep river basins
Dubois, Jérôme; Boillat, Jean-Louis; Schleiss, Anton et al

in 2nd Inter-Regional Conference on Environment-Water (1999)

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See detailA new approach in protein phosphorylation for Nanofitin radiolabelling
Goux, Marine ULg

Poster (2013, December)

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as 89Zr or 68Ga. Despite their important role in the therapeutic ... [more ▼]

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as 89Zr or 68Ga. Despite their important role in the therapeutic world, antibodies have many disadvantages related to their structure making their production difficult and expensive: limited stability, disulfide bridges and post-translational modifications. Moreover, conjugation of chelating agent often occurs on lysines, which is non-regioselective and leads to a heterogeneous mixture of products. In addition, the long clearance of antibodies can be a problem to obtain a good contrast when they are used in imaging. To address these limitations, we developed a chemistry-free system consisting of a highly phosphorylatable peptide tag to chelate stereoselectively radionuclides for PET imaging. To ensure an efficient targeting of the radionuclide, the tag was genetically fused to a Nanofitin®, a protein scaffold developed as an alternative to antibodies. Nanofitins share the advantages of being small, cystein-free, easy to engineer, highly stable both to temperature and pH and expressed with high yield in E. coli. We developed an in vivo phosphorylation system consisting in the co-expression of a Nanofitin® fused to the phosphorylatable tag and the α subunit of casein kinase II, in order to save purification steps and decrease production costs. Effectiveness was demonstrated by electrophoretic mobility shift assay and staining with a specific phosphoprotein staining gel. To further optimize and validate the system, the rate of phosphorylation is evaluated by an isoelectric focusing method (based on pHi variability). In addition, we are developing a phosphoprotein purification protocol based on gallium(III) or iron(III) functionalized chromatography beads. Eventually, the use of a single tag for both the purification and the labeling together with Nanofitins as a highly stable targeting moiety will provide a much simpler and straightforward system. [less ▲]

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See detailNew approach of calculation of dynamics of ball bearings
Bozet, Jean-Luc ULg; Servais, Christophe ULg

Conference (2015, March)

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See detailA new approach of food microbiology with the metagenomic tools: an application on fish
Delhalle, Laurent ULg; Taminiau, Bernard ULg; Nezer, Carine et al

Poster (2012, September 03)

Metagenomics has appeared as a powerful tool to study bacterial composition of various environmental samples. Its interest has started to appear in food microbiology but only on the study of very ... [more ▼]

Metagenomics has appeared as a powerful tool to study bacterial composition of various environmental samples. Its interest has started to appear in food microbiology but only on the study of very particular bacterial populations of fermented food. This work describes the application of this technique to study the bacterial population of two fresh fish filets. The two fish species are from freshwater (pangasius) and seawater (haddock), respectively. Samples where directly analyzed the day of receipt. Others samples were analyzed at the end their shelf life after storage at 4°C (1/3 of their shelf life) and 8°C (2/3 of their shelf life). For these samples, packagings were made in plastic wrap for atmospheric air condition and in trays under modified atmosphere. Classical microbiological and 16S rDNA metagenomic analysis were carried out on all these samples. The composition and evolution of microbial populations of fish filet stored under different packaging conditions and temperatures of storage were investigated with identification of bacteria species. A total of 40 different species were identified for both fish types. Gram-negative bacteria are always predominated among the initial flora and at the end of the shelf life in all the trials. At the beginning of storage, the predominant Gram-negative microflora consisted of Moraxellaceae (Acinetobacter spp, Psychrobacter spp.), Pseudomonadaceae (Pseudomonas spp), and Shewanella spp and the Gram-positive flora was identified as Lactobacillaceae (Carnobacterium spp), Brochothrix thermosphacta and Planococcus donghaensis (only for pangasius). Regardless the packaging and the fish origin, significant variations of the initial flora were noted. The important growth of some Gram negative populations could indicate a risk of spoilage. Thus, the metagenomic approach could be used to adequately determine the duration of shelf-life. For the pangasius, Planococcus donghaensis is only present before the fish is packed and its dominant presence could provide an indication of the freshness of the fish. The metagenomic analysis is a useful tool to identify and to measure the relative proportions of bacterial species in fish filet samples. [less ▲]

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See detailA new approach to analyze genotypes of colony-forming cyanobacteria from environmental samples.
Lara, Yannick ULg; BOUTTE, Christophe; PERETYATKO, Anatoly et al

Poster (2008, August 31)

Several studies have shown the efficiency of sequences as rRNA-ITS, cpcBA, rbcLX and other housekeeping genes to study taxonomy [1, 2, 3], population, community structure of cyanobacteria, or for Multi ... [more ▼]

Several studies have shown the efficiency of sequences as rRNA-ITS, cpcBA, rbcLX and other housekeeping genes to study taxonomy [1, 2, 3], population, community structure of cyanobacteria, or for Multi Locus Sequence Analysis [4]. Recently, the genotypic analysis of single colonies and single filaments directly isolated from the environment has been carried out by other authors. It appears that different genotypes of Microcystis are present in one population in one lake. Besides, succession of toxic and non-toxic genotypes may have a critical influence on toxin concentrations during the blooms [5]. Genotypic analysis of colony-forming cyanobacteria requires enough DNA. So far, the genotypes of environmental single colonies of Microcystis were characterized on the basis of one or two PCR [6]. As the DNA content of one single colony only allows for a few PCR reactions, we have developed a new approach using Whole Genome Amplification with Phi29 polymerase to allow for the Multi Locus Sequences Typing analysis of a single colony or filament. For the first time, we were able to amplify and sequence more than one locus of the genome of a single colony of Microcystis. In addition, we have obtained the first sequences of rpoC1, rbcLX and rRNA-ITS from a single colony of the genus Woronichinia ( identified by microscopy). This approach allows to work with a small amount of DNA, and represents a concrete answer to the lack of data on non-cultivable cyanobacteria. This research is supported by the Belgian Science policy under the science for a sustainable development (SSD) and Fonds de la Recherche Scientifique-FNRS with a FRIA fellowship. References: [1] Otsuka S, et al (1999) FEMS Microbiology Letters 172 15-21 [2] Gugger M, et al (2002) Int J Syst Evol Microbiol 52 1867-1880 [3] Haverkamp T, et al (2008) Environmental Microbiology 10(1) 174-188 [4] Lodders N, et al (2005) Environmental Microbiology 7 (3) 434-442 [5] Kardinaal E, and Visser P (2005) In Harmfuf cyanobacteria, Springer Dordrecht pp 41-64 [6] Janse I, et al (2004) Appl Environm Microbiol 70 (7) 3979–3987 [less ▲]

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See detailA new approach to coil-planet centrifuges for extraction and phase separation in downstream processing
Scherübel, Peter; Pfennig, Andreas ULg

Conference (2014, September 11)

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See detailNew Approach to Description of (d,xn) Spectra at energies below 50 MeV in Monte Carlo Simulation by Intra Nuclear Cascade Code with Distorted Wave Born Approximation
Hashimoto, S.; Iwamoto, Y.; Sato et al

in Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms (2014), 333

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See detailA New Approach to Industrial Market Segmentation
Choffray, Jean-Marie ULg; Lilien, Gary L.

in Sloan Management Review (1978), (Spring), 17-29

This paper presents a methodology to segement industrial markets on the basis of the purchasing process in buying organisations. It proposes a measurement tool, called a decision matrix, and associated ... [more ▼]

This paper presents a methodology to segement industrial markets on the basis of the purchasing process in buying organisations. It proposes a measurement tool, called a decision matrix, and associated statistical methods to perform such analyses. [less ▲]

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See detailNew approach to quantitative analysis of benzo[a]pyrene in food supplements by an immunochemical column test
Beloglazova, Natalia; Goryacheva, Irina; De Saeger, Sarah et al

in Talanta (2011), 85

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See detailNew Approach to Satellite Formation-Keeping: Exact Solution to the Full Nonlinear Problem
Cho, Hancheol ULg; Yu, Adam

in Journal of Aerospace Engineering (2009), 22(4), 445-455

This paper presents a new, simple, and exact solution to the formation keeping of satellites when the relative distance between the satellites is so large that the linearized relative equations of motion ... [more ▼]

This paper presents a new, simple, and exact solution to the formation keeping of satellites when the relative distance between the satellites is so large that the linearized relative equations of motion no longer hold. We employ a recently proposed approach, the Udwadia-Kalaba approach, which makes it possible to explicitly obtain the desired control function without making any approximations related to the nonlinearities in the underlying dynamics. We use an inertial frame of reference to describe the motion of a satellite and since no approximations are made, the results obtained apply to situations even when the distance between the satellites is arbitrarily large. The paper deals with a projected circular formation, but the methodology in this paper can be applied to any desired configuration or orbital requirements. Numerical simulations confirm the brevity and the accuracy of the analytical solution to the dynamical control problem developed herein. [less ▲]

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