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See detailProtective role of P2Y(2) receptor against lung infection induced by pneumonia virus of mice
Vanderstocken, Gilles; Van de Paar, Els; Robaye, Benoit et al

in PLoS ONE (2012), 7(11), 50385

ATP released in the early inflammatory processes acts as a danger signal by binding to purinergic receptors expressed on immune cells. A major contribution of the P2Y2 receptor of ATP/UTP to dendritic ... [more ▼]

ATP released in the early inflammatory processes acts as a danger signal by binding to purinergic receptors expressed on immune cells. A major contribution of the P2Y2 receptor of ATP/UTP to dendritic cell function and Th2 lymphocyte recruitment during asthmatic airway inflammation was previously reported. We investigated here the involvement of P2Y2 receptor in lung inflammation initiated by pneumonia virus of mice infection. We demonstrated that P2Y2-/- mice display a severe increase in morbidity and mortality rate in response to the virus. Lower survival of P2Y2-/- mice was not correlated with excessive inflammation despite the higher level of neutrophil recruiters in their bronchoalveolar fluids. Interestingly, we observed reduced ATP level and lower numbers of dendritic cells, CD4+ T cells and CD8+ T cells in P2Y2-/- compared to P2Y2+/+ infected lungs. Lower level of IL-12 and higher level of IL-6 in bronchoalveolar fluid support an inhibition of Th1 response in P2Y2-/- infected mice. Quantification of DC recruiter expression revealed comparable IP-10 and MIP-3 levels but a reduced BRAK level in P2Y2-/- compared to P2Y2+/+ bronchoalveolar fluids. Higher morbidity and mortality of P2Y2-/- mice appear to result from defective dendritic cell and T cell infiltration that were correlated with higher virus titer. In conclusion, P2Y2 receptor previously described as a target in cystic fibrosis therapy and as a mediator of Th2 response in asthma, may also regulate Th1 response protecting mice against lung viral infection. [less ▲]

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See detailLa « Protectrice du Païs-Bas ». Stratégies politiques et figures de la Vierge dans les Pays-Bas espagnols
Delfosse, Annick ULg

Book published by Brepols Publishers (2009)

Dans les Pays-Bas espagnols, morcelés par les revendications particularistes provinciales et bouleversés par d'incessantes guerres, se construit tout au long du XVIIe siècle une figure originale de la ... [more ▼]

Dans les Pays-Bas espagnols, morcelés par les revendications particularistes provinciales et bouleversés par d'incessantes guerres, se construit tout au long du XVIIe siècle une figure originale de la Vierge. "Reine de Guerre" et "Victorieuse", garante du pouvoir des Habsbourg et Patronne des villes, elle entre dans l'appareil symbolique que forgent les autorités pour promettre aux populations l'ordre espéré et fonder par la même occasion leur légitimité. L'A. repère et analyse les procédés qui ont contribué à définir ce rôle politique d'une Vierge puissante, triomphale et souveraine. Elle montre comment émerge cette fonction mariale tant dans la littérature pèlerine, dévotionnelle et théologique que dans les pratiques des communautés urbaines et de la Cour installée à Bruxelles [less ▲]

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See detailProtégeons la beauté du ciel nocturne-I
Jehin, Emmanuel ULg; Demoulin, Philippe ULg

Article for general public (2000)

Not Available

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See detailProtégeons la beauté du ciel nocturne-II
Jehin, Emmanuel ULg; Demoulin, Philippe ULg

Article for general public (2000)

Not Available

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See detailProtégeons nos mammifères: le point cinq ans après
Libois, Roland ULg

Learning material (1987)

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See detailProtein adsorption on preadsorbed polyampholytic monolayers
Mahltig, Boris; Werner, Carsten; Müller, Martin et al

in Journal of Biomaterials Science. Polymer Edition (2001), 12(9), 995-1010

The adsorption behaviour of five different globular proteins on pure silicon substrates and on preadsorbed polyampholytic monolayers has been investigated as a function of protein concentration. The ... [more ▼]

The adsorption behaviour of five different globular proteins on pure silicon substrates and on preadsorbed polyampholytic monolayers has been investigated as a function of protein concentration. The prelayers were prepared by adsorption of the ampholytic diblock copolymer poly(methacrylic acid)-block-poly ((dimethylamino)ethyl methacrylate) (PMAA-b-PDMAEMA). This polyampholyte adsorbs in densely packed micelles directly from aqueous solution. Ellipsometry was used to determine the amount of adsorbed polyampholyte and protein. While ATR-IR spectroscopy gives information about the adsorption and desorption behaviour of the preadsorbed polyampholytic layer, the lateral structures of the dried films were investigated by scanning force microscopy (SFM). The amount of protein adsorbed was found to be strongly influenced by the preadsorbed polyampholyte compared to the adsorption on the pure silicon substrates. No displacement of the polyampholyte by the proteins was detected. In most cases the protein adsorption was reduced by the preadsorbed polyampholytic layer. The observed trends are explained by the change in electrostatic and hydrophilic characteristics of the substrates. Furthermore, the entropy of adsorption has to be taken into account. [less ▲]

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See detailProtein And Amino Acid Profiles Of Tunisian Deglet Nour And Allig Date Palm Fruit Seeds
Bouaziz, Mohamed; Besbes, Souhail; Blecker, Christophe ULg et al

in Fruits (2008), 63(1),

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See detailProtein and crude fiber determination on ground or whole seeds triticale by near infrared reflectance or transmittance spectroscopy.
Leterme, Pascal; Dardenne, Pierre; Thewis, André ULg et al

in Proceedings of the Second International Tritical Symposium, Passo Fundo (Brazil) (1990)

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See detailProtein and crude fiber determination on tritical: NI and NIT comparison.
Leterme, Pascal; Dardenne, Pierre; Thewis, André ULg et al

in Biston, Robert; Barthiaux-Thill, Nicole (Eds.) Proceedings of the Third International Conference on Near Infrared Spectroscopy, vol.2 (1990)

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See detailProtein composition and agglomeration tendency of gluten isolated from European wheats (Triticum aestivum L.) in a batter system
Roels, S. P.; Sindic, Marianne ULg; Deroanne, Claude et al

in Journal of Agricultural and Food Chemistry (1998), 46(4), 1344-1349

Pilot scale isolation of gluten (with recovery of gluten on 400, 250, and 125 mu sieves) from flour prepared from six European wheat varieties (Apollo, Slejpner, Sperber, Camp Remy, Minaret, and Soissons ... [more ▼]

Pilot scale isolation of gluten (with recovery of gluten on 400, 250, and 125 mu sieves) from flour prepared from six European wheat varieties (Apollo, Slejpner, Sperber, Camp Remy, Minaret, and Soissons) resulted in, on average, gluten yields of 9.6% (4.7-13.2% range). Gluten protein recoveries averaged 63.0% (34.5-85.7% range). Gluten yields and gluten protein recoveries were linearly related and increased when mixing times and baking absorptions required for optimal dough development of the parent flours increased, indicating that there is a relationship between the agglomeration properties of gluten proteins in a batter system and the optimal technological conditions necessary for processing the flours in breadmaking. The Osborne protein fractions in the gluten fractions were determined. The 0.05 M acetic acid soluble (glutenin) fraction was quantitatively the most important fraction (40-46% of gluten Kjeldahl nitrogen) while comparable levels of 70% ethanol soluble (gliadins) and 0.05 M acetic acid insoluble (residue protein) were found (17.9-22.3% of gluten Kjeldahl nitrogen and 22.0-29.0% of gluten Kjeldahl nitrogen for gliadins and residue protein, respectively). With decreasing pore size of the sieves, the level of glutenin in the gluten decreased while the level of gliadins increased. This indicates that, in gluten with good agglomeration properties, the level of glutenins is high and that the agglomeration properties of such proteins (i.e. their tendency to aggregate) strongly determines the agglomeration behavior of the gluten as a whole. [less ▲]

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See detailProtein crystallisation under microgravity conditions: What did we learn on TIM crystallisation from the Soyuz missions?
MAES, D.; DECANNIERE, K.; ZEGERS, I. et al

in Microgravity Science and Technology (2007), XIX(5/6), 90-94

The protein Triose Phosphate Isomerase from the hyperthermophilic organism Thermotoga maritima was crystallised on board of the International Space Station in the framework of the Soyuz missions. In this ... [more ▼]

The protein Triose Phosphate Isomerase from the hyperthermophilic organism Thermotoga maritima was crystallised on board of the International Space Station in the framework of the Soyuz missions. In this paper we report on the scientific results obtained during these flights. Firstly it qas shown that different crystal forms for the same protein in the same crystallisation conditions, what is presumably due to a change in the rate at which supersaturation is achieved. Secondly, the X-ray qualité of the crystals grown in the ISS is superior to their ground control crystals. Mimicking microgravity on ground, by adding a small amourt of gel to avoid convection, also results in an improvement of X-ray quality. Nevertheless our analysis shows that the crystals obtained in this gelled ground environment are of inferior quality as compared to their space homologues. Finally we observed movement of crystals grown in the International Space Station, not only because of g-jitters but also due to residual accelerations. This has an important effect on concentration gradients of precipiants and therefore on the solubility of the protein. [less ▲]

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See detailProtein expression profiling in the African clawed frog Xenopus laevis tadpoles exposed to the polychlorinated biphenyl mixture aroclor 1254.
Gillardin, Virginie; Silvestre, Frederic; Dieu, Marc et al

in Molecular & Cellular Proteomics (2009), 8(4), 596-611

Exposure to environmental pollutants such as polychlorinated biphenyls (PCBs) is now taken into account to partly explain the worldwide decline of amphibians. PCBs induce deleterious effects on developing ... [more ▼]

Exposure to environmental pollutants such as polychlorinated biphenyls (PCBs) is now taken into account to partly explain the worldwide decline of amphibians. PCBs induce deleterious effects on developing amphibians including deformities and delays in metamorphosis. However, the molecular mechanisms by which they express their toxicity during the development of tadpoles are still largely unknown. A proteomics analysis was performed on developing Xenopus laevis tadpoles exposed from 2 to 5 days postfertilization to either 0.1 or 1 ppm Aroclor 1254, a PCB mixture. Two-dimensional DIGE with a minimal labeling method coupled to nanoflow liquid chromatography-tandem mass spectrometry was used to detect and identify proteins differentially expressed under PCBs conditions. Results showed that 59 spots from the 0.1 ppm Aroclor 1254 condition and 57 spots from the 1 ppm Aroclor 1254 condition displayed a significant increase or decrease of abundance compared with the control. In total, 28 proteins were identified. The results suggest that PCBs induce mechanisms against oxidative stress (peroxiredoxins 1 and 2), adaptative changes in the energetic metabolism (enolase 1, glycerol-3-phosphate dehydrogenase, and creatine kinase muscle and brain types), and the implication of the unfolded protein response system (glucose-regulated protein, 58 kDa). They also affect, at least at the highest concentration tested, the synthesis of proteins involved in normal cytogenesis (alpha-tropomyosin, myosin heavy chain, and alpha-actin). For the first time, proteins such as aldehyde dehydrogenase 7A1, CArG binding factor-A, prolyl 4-hydroxylase beta, and nuclear matrix protein 200 were also shown to be up-regulated by PCBs in developing amphibians. These data argue that protein expression reorganization should be taken into account while estimating the toxicological hazard of wild amphibian populations exposed to PCBs. [less ▲]

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See detailThe protein folding challenge in psychrophiles: facts and current issues
Piette, Florence ULg; Struvay, Caroline ULg; Feller, Georges ULg

in Environmental Microbiology (2011), 13

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See detailProtein folding is a rate limiting step for bacterial growth at low temperatures
Piette, Florence; D'Amico, Salvino; Leprince, Pierre ULg et al

Poster (2006)

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See detailProtein folding is a rate limiting step for bacterial growth at low temperatures
D'Amico, Salvino; Piette, Florence; Leprince, Pierre ULg et al

Poster (2006)

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See detailProtein folding is a rate limiting step for bacterial growth at low temperatures.
Piette, Florence; D'Amico, Salvino; Leprince, Pierre ULg et al

Poster (2006)

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See detailProtein Identification and Quantification in Mixtures of highly-modified Proteins
Dobson, Rowan ULg

Doctoral thesis (2013)

The identification and quantification of proteins in highly-modified mixtures using proteomics has been performed. Two research projects have been undertaken which fulfil this aim. The first comprised the ... [more ▼]

The identification and quantification of proteins in highly-modified mixtures using proteomics has been performed. Two research projects have been undertaken which fulfil this aim. The first comprised the development of quantitative methods to detect trace amounts of hazelnut and soy in complex mixtures. A method for the detection and absolute quantification of Cor a 9, a major hazelnut (Corylus avellana) allergen was developed based on mass spectrometry. One hundred and ten hazelnut proteins were detected, five of which were allergens. The peptide chosen for quantification was from Cor a 9 (11S globulin-like protein). Two specific fragmentation reactions were chosen in multiplexed Selected Reaction Monitoring (SRM). Forty three hazelnut food processing imitation samples, varying a range of factors, such as the temperature and incubation time were analysed. A calibration curve was made for cookies. The developed method was for home-made cookies, shop-bought cookies and chocolate. The quantities of Cor a 9 in each sample were determined from the quantification of the target peptide using isotopic dilution with a heavy isotopically labelled peptide. A second peptide with two transitions was also proven to be a possible alternative as a detection method for hazelnut. The presence of soybean allergens in processed food can be detected using the mass spectrometric identification of a soybean peptide which is resistant to the heating and chemical reactions associated with food processing. The proteomic analysis of soybeans allowed the identification of 11 allergens. A method using a peptide (VFDGELQEGR) from glycinin G1 (Gly m 6.0101) was developed for the detection and semi-quantification of the allergen in food samples. Two specific fragmentation pathways were chosen in selected reaction monitoring for unambiguous identification of glycinin G1 and were: 575.3 903.4 Da and 575.3 788.4 Da. Sixteen imitation samples of processed food spiked with soybean were analyzed, where factors such as temperature and incubation time were varied, and the chosen transitions were detected. The developed method was specific for home-made cookies and a shop-bought biscuit. Semi-quantification from both cooked and uncooked cookies was demonstrated. The second comprised the identification and quantification of conotoxins in the venom of Conus textile by the use of isotope coded affinity tagging (ICAT) and label-free quantification. The extreme variety and complexity of the conotoxins has been insufficiently documented and this research demonstrates the varied nature of conotoxins found in different parts of the venom duct and their patterns of expression. Fifteen conotoxins, several with different post-translational modifications (PTMs), were identified and quantified. Distinctive patterns emerged, with the largest group of conotoxins increasing, then peaking in the central6 proximal part, before decreasing; whilst the second largest group peaked in the distal region, generally displaying nothing in the first parts. A new conotoxin, PCCSKLHDNSCCGL*, was sequenced. [less ▲]

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See detailProtein Kinase- and Staurosporine-Dependent Induction of Neurite Outgrowth and Plasminogen Activator Activity in Pc12 Cells
Leprince, Pierre ULg; Bonvoisin, Catherine ULg; Rogister, Bernard ULg et al

in Biochemical Pharmacology (1996), 52(9), 1399-405

We analysed how interactions between protein kinase-dependent intracellular signalling pathways were implicated in the control of the production of tissue-type plasminogen activator (tPA) and the ... [more ▼]

We analysed how interactions between protein kinase-dependent intracellular signalling pathways were implicated in the control of the production of tissue-type plasminogen activator (tPA) and the generation of neurite outgrowth by PC12 cells. To that aim, cells were treated with agents that interact with the trk receptor and with protein kinases A and C. Nerve growth factor induced only the formation of large neurites. The release of the protease and the production of short neurite outgrowth were found to be protein-kinase-A-dependent events that could be enhanced by simultaneous activation of protein kinase C with phorbol ester. At high concentration, staurosporine, a nonselective inhibitor of protein kinases, induced the production of short neurites and mimicked the protein-kinase-A-dependent effect on tPA release. Such a response was not observed with K-252a, an analogue of staurosporine devoid of neurite-outgrowth-promoting activity. The responses to protein kinase A stimulation and the addition of staurosporine, although similar, seemed to occur through an activation of distinct, yet interacting, signalling pathways. In conclusion, tPA release and large neurite outgrowth from PC12 cells are controlled by parallel, albeit interacting, pathways, suggesting that these two potentially antagonistic events in PC12 cell differentiation can be modulated in a concerted way or independently of each other, depending on the activity of several protein kinases. [less ▲]

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See detailThe protein Nod2: an innate receptor more complex than previously assumed.
Lecat, Aurore ULg; Piette, Jacques ULg; Legrand-Poels, Sylvie ULg

in Biochemical Pharmacology (2010), 80(12), 2021-31

For almost 10 years, Nod2 has been known as a cytosolic innate receptor able to sense peptidoglycan from Gram-positive and -negative bacteria and to trigger RIP2- and NF-kappaB-mediated pro-inflammatory ... [more ▼]

For almost 10 years, Nod2 has been known as a cytosolic innate receptor able to sense peptidoglycan from Gram-positive and -negative bacteria and to trigger RIP2- and NF-kappaB-mediated pro-inflammatory and antibacterial response. Mutations in the gene encoding Nod2 in humans have been associated with Crohn's disease (CD). Mechanisms by which Nod2 variants can lead to CD development are still under investigation. The most admitted hypothesis suggests that the impaired function of Nod2 variants in intestinal epithelial and phagocytic cells results in deficiencies in epithelial-barrier function which subsequently lead to increased bacterial invasion and inflammation at intestinal sites. Very recent results have just reinforced this hypothesis by demonstrating that Nod2 wild-type (unlike Nod2 variants) could mediate autophagy, allowing an efficient bacterial clearance and adaptative immune response. Other recent data have attributed new roles to Nod2. Indeed, Nod2 has been shown to activate antiviral innate immune responses involving IRF3-dependent IFN-beta production after viral ssRNA recognition through a RIP2-independent mechanism requiring the mitochondrial adaptor protein MAVS. Recently, Nod2 has been also shown to be exquisitely tuned to detect mycobacterial infections and mount a protective immunity against these pathogens. [less ▲]

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