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See detailLyrics, Music and Voice: a Comparative Semiotics of Emotions in Cole Porter's "Miss Otis Regrets"
Lindenberg Lemos, Carolina ULiege

Conference (2012, June 27)

What makes for an engaging song? One may credit the lyrics, another the melody, but in fact the two components are indissociable (Tatit, 2007). In Cole Porter's “Miss Otis Regrets”, we are introduced to ... [more ▼]

What makes for an engaging song? One may credit the lyrics, another the melody, but in fact the two components are indissociable (Tatit, 2007). In Cole Porter's “Miss Otis Regrets”, we are introduced to the sad account of Miss Otis. The lyrics give us fragments of her story but not from her point of view. How, then, do we relate to her tragedy? In this paper, a comparative semiotic approach to lyrics, music, and voice will be used to demonstrate the narrator is key to this process. It is from the latter's point of view that we approach the story. First, an analysis of the linguistic component, based on Greimas (1983; 1987) and Zilberberg (1988; 2006), will show how the choice of vocabulary and repetitions subtly disagree with the values assumed by the community that condemns Miss Otis. However, non-linguistic components also play a central role. Following Tatit's (1997; 2007) proposals for the semiotic approach to songs, we will investigate how Porter's choices of accents, elongations and the exploration of pitch range emphasize the points that are most filled with emotions in the narrative. Melody and lyrics are shown to work together in the construction of the emotional connotations. Nonetheless, it is only when it is no longer in paper, but in the form of an actual execution, that the song may be said to move. We then explore the well-known rendition of Ella Fitzgerald. Despite the accompanying piano, we focus here on the voice as the place where linguistic and musical components meet. Our analysis compares the recording and the original score in order to assess the level of adequacy and change that the interpreter promoted. Variations in notes, stress, and rhythm may be shown to change the original direction dramatically, while others offer accentuated distinctions that were already in place. In all cases, the singer’s transformations take the song a step further in its emotional contents. Adopting this comparative semiotic approach, we reestablish the chain that extracts the emotions from the narrative, to the music, and to the voice, showing that the path that ensures the emotional attachment of the listener is in the integration of the three components: lyrics, music, and voice. References: Greimas, A. J. (1983). “De la colère: étude de sémantique lexicale” In: Du sens II. Paris: Seuil, p. 225-246. __________ (1987). De l'imperfection. Périgueux: Fanlac. Tatit, L. (1997). Musicando a Semiótica: Ensaios. São Paulo: Annablume. __________ (2007). Semiótica da Canção: Melodia e Letra. São Paulo: Escuta. Zilberberg, C. (1988). Raison et poétique du sens. Paris: PUF. __________ (2006). Eléments de grammaire tensive. Limoges: Pulim. [less ▲]

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See detailThe Lys-Asp-Tyr Triad within the Mite Allergen Der p 1 Propeptide Is a Critical Structural Element for the pH-Dependent Initiation of the Protease Maturation.
Chevigne, Andy; Campizi, Vincenzo; Szpakowska, Martyna et al

in International Journal of Molecular Sciences (2017), 18(5),

The major house dust mite allergen, Der p 1, is a papain-like cysteine protease expressed as an inactive precursor, proDer p 1, carrying an N-terminal propeptide with a unique structure. The maturation of ... [more ▼]

The major house dust mite allergen, Der p 1, is a papain-like cysteine protease expressed as an inactive precursor, proDer p 1, carrying an N-terminal propeptide with a unique structure. The maturation of the zymogen into an enzymatically-active form of Der p 1 is a multistep autocatalytic process initiated under acidic conditions through conformational changes of the propeptide, leading to the loss of its inhibitory ability and its subsequent gradual cleavage. The aims of this study were to characterize the residues present in the Der p 1 propeptide involved in the initiation of the zymogen maturation process, but also to assess the impact of acidic pH on the propeptide structure, the activity of Der p 1 and the fate of the propeptide. Using various complementary enzymatic and structural approaches, we demonstrated that a structural triad K17p-D51p-Y19p within the N-terminal domain of the propeptide is essential for its stabilization and the sensing of pH changes. Particularly, the protonation of D51p under acidic conditions unfolds the propeptide through disruption of the K17p-D51p salt bridge, reduces its inhibition capacity and unmasks the buried residues K17p and Y19p constituting the first maturation cleavage site of the zymogen. Our results also evidenced that this triad acts in a cooperative manner with other propeptide pH-responsive elements, including residues E56p and E80p, to promote the propeptide unfolding and/or to facilitate its proteolysis. Furthermore, we showed that acidic conditions modify Der p 1 proteolytic specificity and confirmed that the formation of the first intermediate represents the limiting step of the in vitro Der p 1 maturation process. Altogether, our results provide new insights into the early events of the mechanism of proDer p 1 maturation and identify a unique structural triad acting as a stabilizing and a pH-sensing regulatory element. [less ▲]

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See detailLys13 plays a crucial role in the functional adaptation of the thermophilic triose-phosphate isomerase from Bacillus stearothermophilus to high temperatures
Alvarez, Marco; Wouters, Johan; Maes, Dominique et al

in Journal of Biological Chemistry (1999), 274(27), 19181-7

The thermophilic triose-phosphate isomerases (TIMs) of Bacillus stearothermophilus (bTIM) and Thermotoga maritima (tTIM) have been found to possess a His12-Lys13 pair instead of the Asn12-Gly13 pair ... [more ▼]

The thermophilic triose-phosphate isomerases (TIMs) of Bacillus stearothermophilus (bTIM) and Thermotoga maritima (tTIM) have been found to possess a His12-Lys13 pair instead of the Asn12-Gly13 pair normally present in mesophilic TIMs. His12 in bTIM was proposed to prevent deamidation at high temperature, while the precise role of Lys13 is unknown. To investigate the role of the His12 and Lys13 pair in the enzyme's thermoadaptation, we reintroduced the "mesophilic residues" Asn and Gly into both thermophilic TIMs. Neither double mutant displayed diminished structural stability, but the bTIM double mutant showed drastically reduced catalytic activity. No similar behavior was observed with the tTIM double mutant, suggesting that the presence of the His12 and Lys13 cannot be systematically correlated to thermoadaptation in TIMs. We determined the crystal structure of the bTIM double mutant complexed with 2-phosphoglycolate to 2.4-A resolution. A molecular dynamics simulation showed that upon substitution of Lys13 to Gly an increase of the flexibility of loop 1 is observed, causing an incorrect orientation of the catalytic Lys10. This suggests that Lys13 in bTIM plays a crucial role in the functional adaptation of this enzyme to high temperature. Analysis of bTIM single mutants supports this assumption. [less ▲]

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See detailA lysine cluster in domain II of Bacillus subtilis PBP4a plays a role in the membrane attachment of this C1 PBP.
Vanden Broeck, Arnaud; Dauvin, Marjorie ULiege; Sauvage, Eric et al

Poster (2015, June 08)

Bacillus subtilis PBP4a belongs to the class-C1 PBPs characterized by two internal additional domains of unknown function. Seven lysine residues (K) are protruding from domain II. Four of them: K86, K114 ... [more ▼]

Bacillus subtilis PBP4a belongs to the class-C1 PBPs characterized by two internal additional domains of unknown function. Seven lysine residues (K) are protruding from domain II. Four of them: K86, K114, K119 and K265 have been mutated in glutamine residues (Q). Both proteins (WT and Mut4KQ PBP4a) have been produced without signal peptide in E. coli and their sub-cellular localizations determined by measuring the DD-carboxypeptidase activities in the different compartments (cytoplasmic vs membrane attached proteins). After purification, their binding to B. subtilis membranes has been compared: WT PBP4a interacts in vitro with membranes isolated from this organism in contrast to Mut4KQ PBP4a that remains entirely unbound. In absence of any amphiphilic peptide in PBP4a, the crown of positive charges on the surface of domain II is likely responsible for the PBP cellular localization in interaction with the cytoplasmic membrane. [less ▲]

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See detailA lysine cluster in domain II of Bacillus subtilis PBP4a plays a role in the membrane attachment of this C1-PBP
Vanden Broeck, Arnaud; Van Der Heiden, Edwige ULiege; Sauvage, Eric ULiege et al

in PLoS ONE (2015)

In PBP4a, a Bacillus subtilis class-C1 penicillin-binding protein (PBP), four clustered lysine (K) residues, K86, K114, K119, and K265, protrude from domain II. Replacement of these amino acids with ... [more ▼]

In PBP4a, a Bacillus subtilis class-C1 penicillin-binding protein (PBP), four clustered lysine (K) residues, K86, K114, K119, and K265, protrude from domain II. Replacement of these amino acids with glutamine (Q) residues by site-directed mutagenesis yielded Mut4KQ PBP4a. When produced in Escherichia coli without its predicted Sec-signal peptide, wildtype (WT) PBP4a was found mainly associated with the host cytoplasmic membrane, whereas Mut4KQ PBP4a remained largely unbound. After purification, the capacities of the two proteins to bind to B. subtilis membranes were compared. The results were similar to those obtained in E. coli: in vitro, a much higher percentage of WT PBP4a than of Mut4KQ PBP4a was found to interact with B. subtilis membranes. Immunodetection of PBP4a in B. subtilis membrane extracts revealed that a processed form of this PBP (as indicated by its size) associates with the B. subtilis cytoplasmic membrane. In the absence of any amphiphilic peptide in PBP4a, the crown of positive charges on the surface of domain II is likely responsible for the cellular localization of this PBP and its attachment to the cytoplasmic membrane. [less ▲]

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See detailLysosomal phospholipases inhibition by emetine, strychnopentamine and usambarensine
Quetin-Leclercq, Joelle; Schomer, G; Van Bambeke, F et al

Poster (1999)

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See detailLysozyme gene activity in chicken macrophages is controlled by positive and negative regulatory elements.
Steiner, Christoph; Muller, Marc ULiege; Baniahmad, Aria et al

in Nucleic Acids Research (1987), 15(10), 4163-78

The chicken lysozyme gene is constitutively active in macrophages and under the control of steroid hormones in the oviduct. To investigate which DNA elements are involved in the control of its expression ... [more ▼]

The chicken lysozyme gene is constitutively active in macrophages and under the control of steroid hormones in the oviduct. To investigate which DNA elements are involved in the control of its expression in macrophages we performed transient DNA transfer experiments with two different types of plasmids: 5'-deletion mutants of the upstream region of the chicken lysozyme gene and different fragments from this area in front of the thymidine kinase promoter (herpes simplex virus), each placed in front of the CAT (chloramphenicol acetyl transferase) coding sequence. Two enhancers (E-2.7 kb and E-0.2 kb) were characterized. They are active in macrophages, but not in chicken fibroblasts. Furthermore a negative element (N-2.4 kb) was identified, which is active in fibroblasts and promyelocytes, but not in mature macrophages. The combined action of all three elements contributes to the observed lysozyme gene activities: no activity in fibroblasts, moderate activity in promyelocytes and high activity in mature macrophages. [less ▲]

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See detailLʼétalement urbain en Europe : un défi environnemental ignoré
Halleux, Jean-Marie ULiege

Article for general public (2007)

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See detailLʼimmobilier de bureau en Belgique, un révélateur des mutations économiques et urbaines ?
Bruck, L.; Mairy, N.; Halleux, Jean-Marie ULiege et al

in Bulletin de la Société Géographique de Liège (2005), 46

The aim of the paper is to analyse how office markets are in interaction with the evolutions in the field of territory and economy. The first part of the paper analyses the data available to study the ... [more ▼]

The aim of the paper is to analyse how office markets are in interaction with the evolutions in the field of territory and economy. The first part of the paper analyses the data available to study the office stocks. Afterwards, we present an urban hierarchy based on the office equipment, in relation with the process of metropolization and its location consequences. The last part of the paper shows that the office function is also concerned with the process of periurbanization. [less ▲]

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See detailM Cushing : a life long experience with pituitary surgery
Beckers, Albert ULiege

Scientific conference (2008, October 07)

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See detailM Dwarf Exoplanet Surface Density Distribution: A Log-Normal Fit from 0.07-400 AU
Meyer, Michael R.; Amara, Adam; Reggiani, Maddalena ULiege et al

in Astronomy and Astrophysics Letters (2017), 1707

We fit a log-normal function to the M dwarf orbital surface density distribution of gas giant planets, over the mass range 1-10 times that of Jupiter, from 0.07-400 AU. We use a Markov Chain Monte Carlo ... [more ▼]

We fit a log-normal function to the M dwarf orbital surface density distribution of gas giant planets, over the mass range 1-10 times that of Jupiter, from 0.07-400 AU. We use a Markov Chain Monte Carlo approach to explore the likelihoods of various parameter values consistent with point estimates of the data given our assumed functional form. This fit is consistent with radial velocity, microlensing, and direct imaging observations, is well-motivated from theoretical and phenomenological viewpoints, and makes predictions of future surveys. We present probability distributions for each parameter as well as a Maximum Likelihood Estimate solution. We suggest this function makes more physical sense than other widely used functions, and explore the implications of our results on the design of future exoplanet surveys. [less ▲]

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See detailM- Service Value Perceptions and Customer Loyalty
Pihlstrom, M.; Liljander, V.; Van Riel, Allard ULiege

Poster (2008, July 14)

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See detailM-Ingestion: Simultaneous Ingestion of Ionosonde and GNSS Data into the NeQuick Model
Bidaine, Benoît ULiege; Nava, Bruno; Stankov, Stanimir et al

in Doherty, P.; Hernández-Pajares, M.; Juan, J.-M. (Eds.) et al The International Beacon Satellite Symposium BSS2010 (2010, June)

The steady increase in volume and quality of ionospheric measurements allows for potential improvements in the existing ionospheric models. In particular ionosonde and Global Navigation Satellite Systems ... [more ▼]

The steady increase in volume and quality of ionospheric measurements allows for potential improvements in the existing ionospheric models. In particular ionosonde and Global Navigation Satellite Systems (GNSS) data provide complementary information for original developments regarding the NeQuick model, a quick-run empirical model of the electron density supplying flexible solutions for various applications. Indeed NeQuick is often used to investigate radiowave propagation issues: among others it has been implemented within the Galileo Single Frequency Ionospheric Correction Algorithm. The model is particularly suited to be used within an optimization procedure called ingestion. In this framework, an "effective ionization level" Az plays the role of solar activity input in order to fit a specific dataset eg of slant Total Electron Content (TEC). However larger discrepancies can then obviously be observed towards other parameters eg the F2-layer critical frequency foF2. This draws the interest for a multiple ingestion - or m-ingestion - scheme implying an adaptation towards several datasets by defining several effective parameters. In our study, we perform m-ingestion of ionosonde and GNSS data providing us with slant TEC. In fact it allows us to constrain the electron density profile slab thickness. For the purpose, a high solar activity period (year 2002) was analysed based on collocated digisonde and EUREF GNSS observations at a mid-latitude site, Dourbes (50.1◦N, 4.6◦E). In this way, we can gather some clues on expected ionospheric effects on Galileo applications during the next solar activity maximum. [less ▲]

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See detailM-N interaction in beam-to-column joints - Development of a design model
Demonceau, Jean-François ULiege; Jaspart, Jean-Pierre ULiege

in Zingoni, Alphose (Ed.) Proceedings of the SEMC 2010 International Conference (2010, September 06)

The component method is a nowadays widely recognized procedure for the evaluation of the design properties of structural joints. It is used as a reference method in the Eurocodes and the proposed rules in ... [more ▼]

The component method is a nowadays widely recognized procedure for the evaluation of the design properties of structural joints. It is used as a reference method in the Eurocodes and the proposed rules in these codes are mainly devoted to the characterization of joints subjected to bending moments and shear forces. However, in some situations, these joints can be subjected to combined axial loads and bending mo-ments, for instance in frames subjected to an exceptional event leading to the loss of a column, situation where significant tying forces can developed in the structural beams above the lost column. In this paper, a design model, founded on the component method, aiming at predicting the behaviour of joints subjected to com-bined axial loads and bending moments is presented. In particular, it is illustrated how this model was vali-dated through comparisons to recent experimental tests performed on steel-concrete composite beam-to-column joints. [less ▲]

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See detailM-type channels selectively control bursting in rat dopaminergic neurons
Drion, Guillaume ULiege; Bonjean, Maxime; Waroux, Olivier ULiege et al

in European Journal of Neuroscience (2010), 31

Detailed reference viewed: 130 (60 ULiège)