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See detailMonoclonal antibodies against bovine growth hormone (bGH) enhance the biological response to GH by prolonging GH binding to its liver receptors.
Beauloye, V.; Muaku, S. M.; Portetelle, Daniel ULg et al

in Endocrinology (1995), 136(Suppl 1), 344

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See detailMonoclonal antibodies against bovine growth hormone (bGH) potentiate GH-induced liver IGF-I synthesis and enhance GH binding to hepatic somatogenic receptors
Massart, Serge; Maiter, Dominique; Adam, Emmanuelle et al

in 73° Annual Meeting of the Endocrine Society (1991)

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See detailMonoclonal antibodies against GARP/TGF-b1 complexes inhibit the immunosuppressive activity of human regulatory T cells in vivo
Cuende, Julia; Liénart, Stéphanie; Dedobbeleer, Olivier et al

in Science Translational Medicine (2015), 7(284), 1-12

Regulatory T cells (Tregs) are essential to prevent autoimmunity, but excessive Treg function contributes to cancer progression by inhibiting antitumor immune responses. Tregs exert contact-dependent ... [more ▼]

Regulatory T cells (Tregs) are essential to prevent autoimmunity, but excessive Treg function contributes to cancer progression by inhibiting antitumor immune responses. Tregs exert contact-dependent inhibition of immune cells through the production of active transforming growth factor–b1 (TGF-b1). On the Treg cell surface, TGF-b1 is in an inactive form bound to membrane protein GARP and then activated by an unknown mechanism. We demonstrate that GARP is involved in this activation mechanism. Two anti-GARP monoclonal antibodies were generated that block the production of active TGF-b1 by human Tregs. These antibodies recognize a conformational epitope that requires amino acids GARP137–139 within GARP/TGF-b1 complexes. A variety of antibodies recognizing other GARP epitopes did not block active TGF-b1 production by Tregs. In a model of xenogeneic graft-versus-host disease in NSG mice, the blocking antibodies inhibited the immunosuppressive activity of human Tregs. These antibodies may serve as therapeutic tools to boost immune responses to infection or cancer via a mechanism of action distinct from that of currently available immunomodulatory antibodies. Used alone or in combination with tumor vaccines or antibodies targeting the CTLA4 or PD1/PD-L1 pathways, blocking anti-GARP antibodies may improve the efficiency of cancer immunotherapy. [less ▲]

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See detailMonoclonal antibodies againstbovine growth hormone (bGH) enhance the biological response to GH by prolonging GH binding to its liver receptors
Beauloye, V.; Muaku, S. M.; Portetelle, Daniel ULg et al

in 77° Annual Meeting of the Endocrine Society (1994)

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See detailMonoclonal antibodies to bovine insulin-like growth factor-binding protein -2 and -3: production and preliminary characterization
Portetelle, Daniel ULg; Massart, Serge; Sneyers, Myriam et al

in Hormone binding proteins: physiology and clinical implications symposium (1995)

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See detailMonoclonal Antibodies To Growth Hormone (Gh) Prolong Liver Gh Binding And Gh-Induced Igf-I/Igfbp-3 Synthesis
Beauloye, V.; Muaku, Sm.; Lause, P. et al

in American Journal of Physiology - Endocrinology and Metabolism (1999), 277(2),

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See detailMonoclonal antibodies to oxytocin: production and characterization
Burgeon, Emmanuel; Chapleur, Michèle; Schoenen, Jean ULg et al

in Journal of Neuroimmunology (1991), 31

To induce a good immune response to oxytocin (OT) we developed a two-step technique to conjugate OT to thyroglobulin (TG) using glutaraldehyde. We obtained 30 hybridomas recognizing OT-ovalbumin ... [more ▼]

To induce a good immune response to oxytocin (OT) we developed a two-step technique to conjugate OT to thyroglobulin (TG) using glutaraldehyde. We obtained 30 hybridomas recognizing OT-ovalbumin conjugates and 16 stable lines. Three monoclonal antibodies were selected for further characterization. One of them (O13) was found to be very specific for OT using three different techniques (EIA, RIA and ICC); it si directed to the C-terminal tripeptide. The other two probably recongize tyrosine-containing epitope(s) also shared by vasopressin and other related nonapeptides. [less ▲]

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See detailMonoclonal antibody from hybrid hybridoma: two specificities in one molecule
Rentier, Bernard ULg; Cloes, Jean-Michel

Conference (1988)

Detailed reference viewed: 11 (1 ULg)
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See detailMonoclonal Antibody Production - The Report And Recommendations Of Ecvam Workshop 23
Marx, U.; Embleton, Mj.; Fischer, R. et al

in Alternatives to Laboratory Animals (1997), 25(2),

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See detailMonoclonal antibody to growth hormone the discovery of a new variant, human placental growth hormone
Hennen, Georges ULg; Frankenne, F.; Closset, J. et al

in Monoclonal antibodies : Basic principles, experimental and clinical applications in endocrinology (1985)

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See detailA Monoclonal Elisa for Bovine Viral Diarrhoea Pestivirus Antigen Detection in Persistently Infected Cattle
Mignon, Bernard ULg; Dubuisson, J.; Baranowski, E. et al

in Journal of Virological Methods (1991), 35(2), 177-188

Detection of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is crucial to controlling mucosal disease. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal ... [more ▼]

Detection of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is crucial to controlling mucosal disease. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies raised against the 48-kDa glycoprotein and the 120/80-kDa protein was developed for detecting antigens in leucocytes of 3 persistently BVDV-infected calves. The test is simple, sensitive and rapid. Moreover the same ELISA was able to recognise Belgian field isolates of BVDV. These results show that the test can be applied in the field. [less ▲]

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See detailMonoclonal-Antibodies To Bovine Growth-Hormone Potentiate Hormonal Activity In-Vivo By Enhancing Growth-Hormone Binding To Hepatic Somatogenic Receptors
Massart, Serge; Maiter, D.; Portetelle, Daniel ULg et al

in Journal of Endocrinology (1993), 139(3),

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See detailMonofluoride Bridged, Binuclear Metallacycles of First Row Transition Metals Supported by Third Generation Bis(1-pyrazolyl)methane Ligands: Unusual Magnetic Properties
Reger, Daniel; Watson, R. P.; Foley, E. A. et al

in Inorganic Chemistry (2009), 48

The reaction of M(BF4)2•xH2O, where M is Fe, Co, Cu, and Zn, and the bitopic, bis(pyrazolyl)methane ligand m-[CH(pz)2]2C6H4, Lm, where pz is a pyrazolyl ring, yields the monofluoride bridged, binuclear ... [more ▼]

The reaction of M(BF4)2•xH2O, where M is Fe, Co, Cu, and Zn, and the bitopic, bis(pyrazolyl)methane ligand m-[CH(pz)2]2C6H4, Lm, where pz is a pyrazolyl ring, yields the monofluoride bridged, binuclear [M2(μ-F)(μ-Lm)2](BF4)3 complexes. In contrast, a similar reaction of Lm with Ni(BF4)2•6H2O yields dibridged [Ni2(μ-F)2(μ-Lm)2](BF4)2. The solid state structures of seven [M2(μ-F)(μ-Lm)2](BF4)3 complexes, with M = Fe, Co, Cu, and Zn, indicate that the divalent metal ion is in a five-coordinate, trigonal bipyramidal, coordination environment with either a linear M–F–M bridging arrangement in five of the complexes, or with a slightly bent Cu–F–Cu bridge in two of the complexes. NMR results indicate that [Zn2(μ-F)(μ-Lm)2](BF4)3 retains its dimeric structure in solution. The [Ni2(μ-F)2(μ-Lm)2](BF4)2 complex has a dibridging fluoride structure that has a six-coordination environment about each nickel(II) ion. In the solid state, the [Fe2(μ-F)(μ-Lm)2](BF4)3 and [Co2(μ-F)(μ-Lm)2](BF4)3 complexes show weak intramolecular antiferromagnetic exchange coupling between the two metal(II) ions with J values of –10.4 and –0.67 cm–1, respectively; there is no observed long-range magnetic order. Three different solvates of [Cu2(μ-F)(μ-Lm)2](BF4)3 are diamagnetic between 5 and 400 K, thus showing strong antiferromagnetic exchange interactions of –600 cm–1 or more negative. Mössbauer spectra indicate that [Fe2(μ-F)(μ-Lm)2](BF4)3 exhibits no long-range magnetic order between 4.2 and 295 K and isomer shifts that are consistent with the presence of five-coordinate, high-spin iron(II). [less ▲]

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See detailMonofrequency Forced Oscillation Technique for the Investigation of Pulmonary Function in Calves: In Vitro and in Vivo Studies
Close, Roland; Reinhold, Petra; Lekeux, Pierre ULg

in Research in Veterinary Science (1994), 56(3), 363-372

Parameters derived from the non-invasive and simple monofrequency forced oscillation technique were compared with classical parameters of ventilatory mechanics in order to assess its usefulness for the ... [more ▼]

Parameters derived from the non-invasive and simple monofrequency forced oscillation technique were compared with classical parameters of ventilatory mechanics in order to assess its usefulness for the investigation of pulmonary function in calves. To facilitate this comparison, theoretical derivations were coupled with in vitro measurements, using an artificial lung model, and with in vivo studies. These studies compared the oscillatory resistance parameters (Ros and Re) and the respiratory system compliance (Crs) against the classical pulmonary resistance (RL) and the dynamic compliance (Cdyn), respectively. Ros and Re were highly correlated (r > or = 0.87) with RL and the comparison between Crs and Cdyn gave a similarly high correlation (r > or = 0.88). Given its simplicity, its correspondence with classical parameters and its rapidity and reproducibility, monofrequency forced oscillation technique seems well suited for the investigation of pulmonary function under field conditions. [less ▲]

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See detailDie monofrequente Oszilloresistometrie - Möglichkeiten und Grenzen eines nichtinvasiven Verfahrens zur Atemwiderstandsmessung beim Kalb
Reinhold, Petra; Close, Roland; Lekeux, Pierre ULg

in Deutsche Tierarztliche Wochenschrift (1994), 101(4), 146-150

The monofrequency forced oscillation technique (MFOT) has been found to be a simple, reproducible, fast and accurate method to carry out lung function testing in calves even under field conditions. If ... [more ▼]

The monofrequency forced oscillation technique (MFOT) has been found to be a simple, reproducible, fast and accurate method to carry out lung function testing in calves even under field conditions. If some technical as well as methodological requirements are taken into account, pulmonary or respiratory resistance can be assessed by means of the parameter oscillatory resistance (Ros). Furthermore, several Ros derived parameters are available for describing the functional status of the central bronchial system. Due to the low variability caused by methodological or intraindividual reasons, the MFOT represents a very qualified method for intraindividual studies. Comparing measuring results of different subjects, a higher biologically caused variance has to be taken into account. [less ▲]

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See detailThe Monofunctional Glycosyltransferase of Escherichia Coli Localizes to the Cell Division Site and Interacts with Penicillin-Binding Protein 3, FtsW, and FtsN
Derouaux, Adeline ULg; Wolf, Benoît ULg; Fraipont, Claudine ULg et al

in Journal of Bacteriology (2008), 190(5), 1831-4

The monofunctional peptidoglycan glycosyltransferase (MtgA) catalyzes glycan chain elongation of the bacterial cell wall. Here we show that MtgA localizes at the division site of Escherichia coli cells ... [more ▼]

The monofunctional peptidoglycan glycosyltransferase (MtgA) catalyzes glycan chain elongation of the bacterial cell wall. Here we show that MtgA localizes at the division site of Escherichia coli cells that are deficient in PBP1b and produce a thermosensitive PBP1a and is able to interact with three constituents of the divisome, PBP3, FtsW, and FtsN, suggesting that MtgA may play a role in peptidoglycan assembly during the cell cycle in collaboration with other proteins. [less ▲]

Detailed reference viewed: 46 (12 ULg)