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See detailModulating effects of acepromazine on the reactive oxygen species production by stimulated equine neutrophils
Sandersen, Charlotte ULg; Mouithys-Mickalad, Ange ULg; de la Rebière de Pouyade, Geoffroy ULg et al

in Veterinary Anaesthesia & Analgesia (2011), 38

To investigate the effect of acepromazine (ACP) on reactive oxygen species (ROS) production by stimulated equine neutrophils.

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See detailModulating mouse innate immunity to RNA viruses by expressing the Bos taurus Mx system.
Garigliany, Mutien-Marie ULg; Cloquette, Karine ULg; Leroy, Michael et al

in Transgenic Research (2009), 18(5), 719-32

Mx proteins are interferon-induced members of the dynamin superfamily of large guanosine triphosphatases. These proteins have attracted much attention because some display antiviral activity against ... [more ▼]

Mx proteins are interferon-induced members of the dynamin superfamily of large guanosine triphosphatases. These proteins have attracted much attention because some display antiviral activity against pathogenic RNA viruses, such as members of the orthomyxoviridae, bunyaviridae, and rhabdoviridae families. Among the diverse mammalian Mx proteins examined so far, we have recently demonstrated in vitro that the Bos taurus isoform 1 (boMx1) is endowed with exceptional anti-rabies-virus activity. This finding has prompted us to seek an appropriate in vivo model for confirming and evaluating gene therapy strategies. Using a BAC transgene, we have generated transgenic mouse lines expressing the antiviral boMx1 protein and boMx2 proteins under the control of their natural promoter and short- and long-range regulatory elements. Expressed boMx1 and boMx2 are correctly assembled, as deduced from mRNA sequencing and western blotting. Poly-I/C-subordinated expression of boMx1 was detected in various organs by immunohistochemistry, and transgenic lines were readily classified as high- or low-expression lines on the basis of tissue boMx1 concentrations measured by ELISA. Poly-I/C-induced Madin-Darby bovine kidney cells, bovine turbinate cells, and cultured cells from high-expression line of transgenic mice were found to contain about the same concentration of boMx1, suggesting that this protein is produced at near-physiological levels. Furthermore, insertion of the bovine Mx system rendered transgenic mice resistant to vesicular-stomatitis-virus-associated morbidity and mortality, and embryonic fibroblasts derived from high-expression transgenic mice were far less permissive to the virus. These results demonstrate that the Bos taurus Mx system is a powerful anti-VSV agent in vivo and suggest that the transgenic mouse lines generated here constitute a good model for studying in vivo the various antiviral functions-known and yet to be discovered-exerted by bovine Mx proteins, with priority emphasis on the antirabic function of boMx1. [less ▲]

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See detailModulating skeletal muscle mass by postnatal, muscle-specific inactivation of the myostatin gene.
Grobet, Luc ULg; Pirottin, Dimitri ULg; Farnir, Frédéric ULg et al

in Genesis (2003), 35(4), 227-38

By using a conditional gene targeting approach exploiting the cre-lox system, we show that postnatal inactivation of the myostatin gene in striated muscle is sufficient to cause a generalized muscular ... [more ▼]

By using a conditional gene targeting approach exploiting the cre-lox system, we show that postnatal inactivation of the myostatin gene in striated muscle is sufficient to cause a generalized muscular hypertrophy of the same magnitude as that observed for constitutive myostatin knockout mice. This formally demonstrates that striated muscle is the production site of functional myostatin and that this member of the TGFbeta family of growth and differentiation factors regulates muscle mass not only during early embryogenesis but throughout development. It indicates that myostatin antagonist could be used to treat muscle wasting and to promote muscle growth in man and animals. [less ▲]

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See detailModulation and mechanism of action of BPDZ 44 on ATP-sensitive potassium channels in isolated rat insulin-secreting cells
Kane, C.; Harding, E. A.; Pirotte, Bernard ULg et al

in Journal of Physiology (1995), 482 P

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See detailModulation by intracellular calcium of a GABA receptor-mediated chloride current via a calcium/calmodulin-dependent protein kinase in insect neurosecretory cells
Alix, Philippe ULg; Grolleau, Françoise; Hue, Bernard

in European Journal of Neuroscience (2000), 12

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See detailModulation de l'effet scale-down chez Saccharomyces cerevisiae par ajout d'acide oléique
Zune, Quentin ULg

Master's dissertation (2011)

Scale-down technology allows to simulate heterogeneities created in an industrial reactor at a laboratory scale. In this kind of bioreactor, fed-batch production of Saccharomyces cerevisiae on glucose ... [more ▼]

Scale-down technology allows to simulate heterogeneities created in an industrial reactor at a laboratory scale. In this kind of bioreactor, fed-batch production of Saccharomyces cerevisiae on glucose induces the apparition of the scale-down effect. It means a decrease in biomass synthesis and an increase in ethanol production as time of culture in comparison with an ideal bioreactor where the mixing operation is perfect. In a recent study, FERIA-GERVASIO et al., (2008) notice that ethanol production is delayed on behalf biomass synthesis when a glucose pulse occurs in a chemostat glucose/oleic acid. The goal of this work is to study the modulation of the scale-down effect by the presence of oleic acid in a fed-batch production of Saccharomyces cerevisiae (boulardii). The methodology is separated in three parts: - development of a yeast conditioning protocol with oleic acid - yeast production with a mixed substrate composed of glucose and oleic acid in a scale-down reactor - computer simulation of glucose gradients experienced by yeast during their displacement in the bioreactor In addition to the measurements performed to describe growth kinetic of yeast on this mixed substrate, an original phenotypic analysis of yeast has been achieved throughout each culture thanks to flow cytometry. At the end of this work, vacuoles staining with fluoro-isothiocyanate has revealed the induction of peroxisomes infered during the yeast conditioning by flow cytometry. Then, it was demonstrated that in a culture in a scale-down reactor preceded by the conditioning, oleic acid naturally reduces ethanol production for the benefit of the biomass synthesis in comparison with a yeast cultivated on glucose only in scale-down reactors. Flow cytometry has not identified a negative influence of oleic acid on cell viability. Finally, simulation of glucose gradients experienced by yeast in the scale-down reactor with oleic acid has demonstrated that yeast feel important extracellular fluctuations in the recycle loop. [less ▲]

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See detailModulation de la dose du fongicide appliqué au stade dernière feuille pour lutter contre les maladies foliaires de l'orge d'hiver en Belgique
Meeùs, Patrick; Bodson, Bernard ULg

in Mededelingen van de Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen (Rijksuniversiteit te Gent) (1994, May 03)

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See detailModulation de la voie de la lipoxygénase dans le cadre de la résistance systémique induite chez la tomate
Mariutto, Martin ULg

Master of advanced studies dissertation (2008)

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See detailModulation des points de contrôle du cycle cellulaire par la protéine Tax du virus T-lymphotrope humain de type 1 (HTLV-1) : vers une nouvelle approche thérapeutique
Carpentier, Alexandre ULg

Master's dissertation (2011)

HTLV-1 infects approximately 20 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is ... [more ▼]

HTLV-1 infects approximately 20 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is currently no satisfactory treatment for these diseases. Among the proteins encoded by HTLV-1, Tax appears to play an important role in the mechanisms leading to pathogenicity. In this work, we used confocal microscopy and flow cytometry to study the interactions between Tax and the DNA damage response (DDR) as well as the associated DNA repair pathways. We demonstrated that expression of Tax results in arrest of the cell cycle and concomitantly in activation of the ATM-Chk2-p53 axis of the DDR. We showed the involvement of p53 and Chk2 in G1 and S/G2 arrests, respectively. For the sake of biological relevance, we also showed that HTLV-1 infected cells do not encounter any arrest during their proliferation. It seems that these cells are adapted to the p53/Chk2 checkpoints and retain repair pathway(s) controlled by Chk1 and BRCA1. Our results indicate that the Chk1-related trans-lesion synthesis is one of the alternative repair pathways occurring in infected cells. Inhibition of this pathway could be a new therapeutic approach for ATL. [less ▲]

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See detailLa Modulation du libertinage chez Voltaire
Tilkin, Françoise ULg

in Les Cahiers des paralittératures (2005), 9

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See detailModulation du stress oxydant : nouveaux concepts pour de nouvelles applications
PINCEMAIL, Joël ULg

Scientific conference (2016, May 31)

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See detailModulation if growth hormone action by active immunization in dairy cows
Vleurick, Lieve; Deaver, Daniel; Bertozzi, Carlo et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2000), 4

Detailed reference viewed: 12 (0 ULg)