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See detailProtéinas placentárias em mamíferos
Melo de Sousa, Noelita; de Figueiredo, José Ricardo; Gonçalves, Paulo Bayard Dias et al

in Ciência Animal (1997), 7(1), 43-55

During the past twenty years, several new placental proteins have been isolated and characterized in woman and several animal species. Among these proteins, it can be mentioned chorionic gonadotrophins ... [more ▼]

During the past twenty years, several new placental proteins have been isolated and characterized in woman and several animal species. Among these proteins, it can be mentioned chorionic gonadotrophins, placental lactogens, interferon tau, pregnancy specific and pregnancy-associated glycoproteins. In spite of the importance of these studies, there are few papers that describe fundamental aspects of these placental proteins such as physical-chemical pregnancy diagnosis methods. Thus, the aim of this report is to present an overview on the major aspects of placental proteins studied up to now, as well as to consider perspectives for their applications in the future in veterinary practice. [less ▲]

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See detailLa protéine JNKBP1 agit comme un régulateur négatif de la signalisation de NOD2 en inhibant son processus d’oligomérisation
Lecat, Aurore ULg

Doctoral thesis (2012)

Le récepteur cytoplasmique NOD2 est l'un des membres les mieux caractérisés de la famille des NLRs. NOD2 est capable de détecter le muramyldipeptide (MDP), un composant de la paroi bactérienne, ce qui ... [more ▼]

Le récepteur cytoplasmique NOD2 est l'un des membres les mieux caractérisés de la famille des NLRs. NOD2 est capable de détecter le muramyldipeptide (MDP), un composant de la paroi bactérienne, ce qui induit les différentes cascades de signalisation conduisant à l'activation de NF-κB, des MAPKs et de l'autophagie. Ces voies contribuent à une réponse immunitaire innée et adaptative efficace. La perte de fonction des mutants NOD2 a été associée à une plus grande susceptibilité à la maladie de Crohn, ce qui souligne l'importance physiologique de la régulation de l'activité de NOD2. Nous avons effectué une étude par une approche protéomique pour rechercher de nouveaux régulateurs de NOD2. Nous avons généré un modèle cellulaire pour cette étude, les cellules HEK293GNV. Nous avons identifié plusieurs nouveaux partenaires de NOD2, dont la protéine JNKBP1 (c-Jun N-terminal kinase binding protein 1), une protéine scaffold caractérisée par un domaine WD40 en amino-terminal. Nous avons aussi débuté la caractérisation d’autres protéines appartenant aux complexes NOD2 purifiés comme ROCK2 (Rho activated kinase 2) et HDAC5 (Histone deacetylase 5). Au vu de nos premiers résultats, les protéines ROCKs sembleraient être des activateurs de la voie NOD2. Nous nous sommes principalement consacrés à l’étude de JNKBP1 qui a été identifiée, en 1999, comme un partenaire et un régulateur positif de JNK. Nous avons montré que JNKBP1 via son domaine WD40, se lie à NOD2 suite à l’activation par le MDP. Cette interaction atténuait l'activation de NF-κB dépendante de NOD2, la synthèse de l'IL-8 et l’activité antibactérienne de NOD2. JNKBP1 exerçait son effet répresseur en perturbant l’oligomérisation NOD2 et la phosphorylation de la tyrosine de RIP2 : deux étapes nécessaires à la signalisation en aval. En outre, nous avons montré que JNKBP1 et NOD2 étaient exprimées dans l'épithélium intestinal humain et dans les cellules immunitaires recrutées dans la lamina propria, ce qui suggère que JNKBP1 contribuerait au maintien de l'homéostasie intestinale dépendante de NOD2. [less ▲]

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See detailLa protéine P53, protectrice de l'intégrité du génome: rôle dans la genèse des cancers
Bellahcene, Akeila ULg; Merville, Marie-Paule ULg; Gielen Jacques et al

in Revue Médicale de Liège (1994), 49(5), 274-84

L'homéostasie des tissus de l'organisme est le résultat d'un équilibre dynamique stable entre des facteurs de régulation positifs et négatifs. Ceux-ci contrôlent la prolifération des cellules et ... [more ▼]

L'homéostasie des tissus de l'organisme est le résultat d'un équilibre dynamique stable entre des facteurs de régulation positifs et négatifs. Ceux-ci contrôlent la prolifération des cellules et déterminent leur appartenance tissulaire. Des données récentes indiquent que l'apparition des cancers résulte du déséquilibre de cet état dynamique soit part l'activation de facteurs positifs désignés sous le terme d'oncogènes, soit par l'inactivation de facteurs négatifs qui sont regroupés dans la famille des gènes suppresseurs de tumeurs. Le gène codant pour la protéine p53 est un membre particulièrement important de cette dernière famille. En effet, son inactivation, par mutation et/ou délétion, est l'une des altérations génétiques la plus fréquemment détectée dans les cancers. La fonction principale attribuée à la protéine p53 consiste à préserver le génome des altérations susceptibles d'entraîner, entre autres, la cellule dans un processus de transformation maligne. C'est en permettant la réparation des altérations du DNA survenues lors de sa réplication avant la mitose ou provoquées par des agents extérieurs, que la protéine p53 semble exercer ses fonctions. Des altérations du gène p53 entraînant des perturbations de la fonction de la protéine p53 ont été identifiées au niveau de la plupart des lésions tumorales malignes. A ce titre, cette protéine semble jouer un rôle déterminant au niveau de la genèse des cancers. Aussi le gène p53 fait-il l'objet de recherches intensives qui devraient déboucher sur la mise au point de nouveaux moyens de détection et d'évaluation pronostique des cancers. D'autre part, des expériences visant à restaurer la fonction de la protéine p53 au niveau des cellules cancéreuses ouvrent de nouvelles et séduisantes perspectives thérapeutiques. [less ▲]

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See detailProtéines alimentaires et fonction rénale
Krzesinski, Jean-Marie ULg

Conference (2001, September 19)

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See detailLes protéines de choc thermique (heat shock proteins). II Les protéines de choc thermique (heat shock proteins). II. Hsp70, biomarqueur, bioprotecteur.
Wirth, Delphine; Gustin, Pascal ULg; Drion, Pierre ULg et al

in Annales de Médecine Vétérinaire (2003), 147(2), 127-144

The exposure of any organism to high temperature induces rapid and transient cellular overexpression of specific proteins, the heat shock proteins (Hsps). This response, called heat shock response, was ... [more ▼]

The exposure of any organism to high temperature induces rapid and transient cellular overexpression of specific proteins, the heat shock proteins (Hsps). This response, called heat shock response, was initially discovered in Drosophila. The genes hsps were among the first eukaryotic genes to be cloned and whose regulation, which involves activation of heat shock factor (HSF), was elucidated. More recently, study of Hsp functions, particularly Hsp70, started. Their protective role, related to their molecular chaperon function, was inferred from experiments showing that Hsp70 expression, induced by a first stress, results in a cellular tolerance to second stress. Better understanding of the heat shock response had led to the development of two search fields on Hsp70 expression : (1) its use as biomarker of cellular stress and (2) the exploitation of its cytoprotective functions against various insults. This review includes historic of the research on the heat shock response, description of regulation mechanism of Hsp70 expression, and the interesting perspectives allocated to Hsp70 as biomarker and therapeutic tool. ----------- L’exposition de tout organisme à des températures élevées induit l'expression cellulaire rapide et transitoire de protéines spécifiques, les protéines de choc thermique (Hsps pour " heat shock proteins "). Cette réponse des cellules au choc thermique ou " heat shock response " a été initialement découverte chez la drosophile. Les gènes hsps furent parmi les premiers gènes eucaryotes à être clonés et utilisés comme paradigme dans l'étude des mécanismes de régulation transcriptionnelle faisant intervenir l'activation d'un ou plusieurs " heat shock factors" (HSFs). C'est plus récemment que l'étude des fonctions des Hsps, particulièrement Hsp70, a débuté. Le rôle protecteur de celle-ci, lié à sa fonction de " chaperon " protéique, a été déduit d'expériences montrant que l'induction de l'expression de Hsp70 lors d'un stress était associée au développement d'une tolérance cellulaire vis-à-vis d'un stress ultérieur. En plus d'un intérêt fondamental pour la compréhension de la "réponse au choc thermique", deux axes de recherche appliquée se sont développés visant à investiguer la possibilité d'utiliser l'expression de Hsp70 comme biomarqueur de souffrance cellulaire d'une part et, d'autre part, d'exploiter ses fonctions comme moyen de protection des cellules contre divers types d'agressions. Cette revue décrit l'historique des découvertes sur la " réponse au choc thermique ", le mécanisme régulant l'expression de Hsp70 ainsi que les perspectives intéressantes s'ouvrant à l'utilisation de l'expression de Hsp70 en tant que biomarqueur et outil thérapeutique. [less ▲]

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See detailLes proteines SIBLING - Outils moleculaires de la progression tumorale et de l'angiogenese.
Lamour, Virginie; Nokin, Marie-Julie ULg; Henry, Aurélie ULg et al

in Medecine sciences : M/S (2013), 29(11), 1018-25

The small integrin-binding ligand N-linked glycoprotein (SIBLING) family consists of osteopontin (OPN), bonesialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP) and matrix ... [more ▼]

The small integrin-binding ligand N-linked glycoprotein (SIBLING) family consists of osteopontin (OPN), bonesialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP) and matrix extracellular phosphoglycoprotein (MEPE). These proteins, initially identified in bone and teeth, share many structural characteristics. It is now well established that they are over expressed in many tumors and play a critical role at different steps of cancer development. In this review, we describe the roles of SIBLING proteins at different stages of cancer progression including cancer cell adhesion, proliferation, migration, invasion, metastasis and angiogenesis. [less ▲]

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See detailProteinose alveolaire: restauration fonctionnelle des macrophages alveolaires apres lavage therapeutique.
Bury, Thierry ULg; CORHAY, Jean-Louis ULg; Saint-Remy, P et al

in Revue des Maladies Respiratoires (1989), 6(4), 373-5

The aim of this study was to specify the effect of therapeutic pulmonary lavage on the function of alveolar macrophages in a patient suffering from alveolar proteinosis; we have studied phagocytosis and ... [more ▼]

The aim of this study was to specify the effect of therapeutic pulmonary lavage on the function of alveolar macrophages in a patient suffering from alveolar proteinosis; we have studied phagocytosis and chemotaxis in cells before and at different times after the lavage. Our results indicate a restoration of the functional properties of the alveolar macrophages in parallel with an improvement in pulmonary function and blood gases under the effects of treatment. These data are in favour with an inhibitory effect of the lipoprotein material on the function of alveolar macrophages and suggest that the deposit of this material is a determining factor in the alteration in the defense mechanisms of the lung. [less ▲]

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See detailPROTEINS FOR FUSING THE SUB-UNIT B OF THE CHOLERAIC TOXIN AND HETEROLOGOUS ANTIGEN, AND NUCLEIC ACIDS ENCODING THEM.
Lhoir, Cécile; Renard, André; Martial, Joseph ULg

Patent (1990)

The invention relates to a hybrid protein which fuses the sub-unit B of the choleraic toxin with an active sequence of a heterologous antigen with respect to said unit. Said hybrid protein is comprised of ... [more ▼]

The invention relates to a hybrid protein which fuses the sub-unit B of the choleraic toxin with an active sequence of a heterologous antigen with respect to said unit. Said hybrid protein is comprised of a sequence of said sub-unit which extends between the 3rd and 100th amino acid rest of the complete sub-unit B. The heterologous amino acid sequence is used upstream or downstream of the latter. This hybrid protein is usable for vaccination, particularly to help the stabilisation of heterologous antigens in the intestinal environment. [less ▲]

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See detailProteins in the gas phase
Meyer, Tim; Gabelica, Valérie ULg; Grubmüller, Helmut et al

in Wiley Interdisciplinary Reviews: Computational Molecular Science (in press)

Proteins are complex macromolecules that evolved over billions of years to be active in aqueous solution. Water is a key element that stabilizes their structure, and most structural studies on proteins ... [more ▼]

Proteins are complex macromolecules that evolved over billions of years to be active in aqueous solution. Water is a key element that stabilizes their structure, and most structural studies on proteins have thus been carried out in aqueous environment. However, recent experimental approaches have opened the possibility to gain structural information on proteins from gas-phase measurements. The obtained results revealed significant structural memory in proteins when transferred from water to the gas phase. However, after several years of experimental and theoretical research, the nature of the structural changes induced by vaporization, the exact characteristics of proteins in the gas phase, and the physicochemical forces stabilizing dehydrated proteins are still unclear. We will review here these issues using both experimental and theoretical sources of information. [less ▲]

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See detailProteins of Bovine Herpesvirus Type 4 Released into the Culture Medium of Productively Infected Cells: Identification of a 135k Glycoprotein Involved in Viral Attachment
Dubuisson, J.; Koromyslov, I.; Pastoret, Paul-Pierre ULg et al

in Journal of General Virology (The) (1992), 73((Pt 1)), 189-94

Three bovine herpesvirus type 4 (BHV-4) proteins released into the culture medium of infected cells were identified, with Mr values of 135K, 16K and 14.5K. Among these three proteins, two were ... [more ▼]

Three bovine herpesvirus type 4 (BHV-4) proteins released into the culture medium of infected cells were identified, with Mr values of 135K, 16K and 14.5K. Among these three proteins, two were precipitated by the monoclonal antibodies characterized in this work. One is a glycoprotein of 135K (gp8) which does not seem to be involved in BHV-4 neutralization. Moreover, this 135K glycoprotein adsorbed onto uninfected susceptible cells. The attachment of gp8 to cells was totally inhibited by the prior adsorption of unlabelled viral proteins. Moreover, anti-gp8 monoclonal antibodies were effective in inhibiting the adsorption of gp8. These results indicate that gp8 could be involved in BHV-4 attachment. [less ▲]

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See detailProteins Specified by Bovine Herpesvirus Type 4: Structural Proteins of the Virion and Identification of Two Major Glycoproteins by Using Monoclonal Antibodies
Dubuisson, Jean; Boulanger, Denise; Bublot, Michel et al

in Journal of General Virology (1989), 70 (Pt 7)

Bovine herpesvirus type 4 proteins were identified by PAGE of [35S]methionine- or [3H]glucosamine-labelled purified virions. Thirty-one monoclonal antibodies (MAbs) raised against the V. Test strain were ... [more ▼]

Bovine herpesvirus type 4 proteins were identified by PAGE of [35S]methionine- or [3H]glucosamine-labelled purified virions. Thirty-one monoclonal antibodies (MAbs) raised against the V. Test strain were used to identify 29 proteins, ten of which were glycosylated. All of these glycoproteins belonged to the viral envelope and a 140K non-glycosylated protein appeared to be the major nucleocapsid protein. The MAbs were classified into two groups. The first group precipitated three glycoproteins of Mr 150K, 120K and 51K. The 120K and 51K glycoproteins were linked by disulphide bonds and the 150K glycoprotein was linked to the others by non-covalent bonds. The second group precipitated a different 120K glycoprotein. [less ▲]

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See detailProtelos – unrivalled efficacy in the short-and long-term
Reginster, Jean-Yves ULg

in OsteOpinion (2008), 4

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See detailProtene del latte: le protein del futuro
Paquot, Michel ULg

in Le protein del latte e derivati: verita nutrizionali Masson (1994)

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See detailThe proteolytic activity of MT4-MMP is required for its proangiogenic and pro-metastatic promoting effects
Host, Lorin; Paye, Alexandra ULg; Detry, Benoît ULg et al

in International Journal of Cancer = Journal International du Cancer (2012), 131(7), 1537-1548

MT4-MMP expression in breast adenocarcinoma stimulates tumor growth and metastatic spreading to the lung. However whether these pro-tumorigenic and pro-metastatic effects of MT4-MMP are related to a ... [more ▼]

MT4-MMP expression in breast adenocarcinoma stimulates tumor growth and metastatic spreading to the lung. However whether these pro-tumorigenic and pro-metastatic effects of MT4-MMP are related to a proteolytic action is not known yet. Through site directed mutagenesis MT4-MMP has been inactivated in cancer cells through Glutamic acid 249 substitution by Alanine in the active site. Active MT4-MMP triggered an angiogenic switch at day 7 after tumor implantation and drastically accelerated subcutaneous tumor growth as well as lung colonization in RAG -/- mice. All these effects were abrogated upon MT4-MMP inactivation. In sharp contrast to most MMPs being primarily of stromal origin, we provide evidence that tumor-derived MT4-MMP, but not host-derived MT4-MMP contributes to angiogenesis. A genetic approach using MT4-MMP-deficient mice revealed that the status of MT4-MMP produced by host cells did not affect the angiogenic response. Despite of this tumor intrinsic feature, to exert its tumor promoting effect, MT4-MMP requires a permissive microenvironment. Indeed, tumor-derived MT4-MMP failed to circumvent the lack of an host angio-promoting factor such as lasminogen activator inhibitor (PAI-1). Overall, our study demonstrates the key contribution of MT4-MMP catalytic activity in the tumor compartment, at the interface with host cells. It identifies MT4-MMP as a key intrinsic tumor cell determinant that contributes to the elaboration of a permissive microenvironment for metastatic dissemination [less ▲]

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See detailProteolytic and depilatory activities of actinomycetin
Ghuysen, Jean-Marie ULg

in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1954), 148

cf. C.A. 47, 6491c; 49, 422d. Actinomycetin, obtained from Streptomyces albus G, was shown to contain 5 distinct enzymes which attack, resp., heat-killed Escherichia coli, keratin, mucin, casein, and a ... [more ▼]

cf. C.A. 47, 6491c; 49, 422d. Actinomycetin, obtained from Streptomyces albus G, was shown to contain 5 distinct enzymes which attack, resp., heat-killed Escherichia coli, keratin, mucin, casein, and a component of the epidermis the destruction of which results in epidermal desquamation and loosening of the hair. The substrate of this last enzyme is a complex protein component of the skin extractable by urea soln. (with or without KCl) at 25°. Another fraction of the epidermis, extractable under similar conditions at 0°, appears to be sensitive to the keratinolytic enzyme. [on SciFinder(R)] [less ▲]

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See detailProteolytic Breakdown Of Gliadin By Enterococcus Faecalis Isolated From Tunisian Fermented Dough
M'Hir, S.; Aldric, Jean-Marc ULg; El Mejdoub, Thami ULg et al

in World Journal of Microbiology & Biotechnology (2008), 24(12),

The aim of this work was to select strains with proteolytic activity on wheat gliadin, among lactic acid bacteria, previously isolated from Tunisian fermented wheat dough. Hydrolysis of gliadin, as sole ... [more ▼]

The aim of this work was to select strains with proteolytic activity on wheat gliadin, among lactic acid bacteria, previously isolated from Tunisian fermented wheat dough. Hydrolysis of gliadin, as sole nitrogen source, in an agar medium was visualized by a clear zone surrounding colonies. The increase in absorbance due to gliadin breakdown was measured spectrophotometrically using Ophthaldialdehyde (OPA) on Gliadin Glucose Broth medium. Fermented liquid dough inoculated with individual selected Enterococcus faecalis, showed a decrease of the gliadin concentration from 45 g/kg to 18 g/kg determined by sandwich ELISA test (R-7001). Only the enterococci strains show an hydrolysis of gliadin proteins. Strains showing proteolytic activity are gaining more and more importance in cereal based fermented foods and may help to reduce gliadin involved in coeliac disease. [less ▲]

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See detailProteolytic cleavage confers nitric oxide synthase inducing activity upon prolactin
Corbacho, A. M.; Nava, G.; Eiserich, J. P. et al

in Journal of Biological Chemistry (2000), 275(18), 13183-6

Prolactin (PRL), originally associated with milk secretion, is now known to possess a wide variety of biological actions and diverse sites of production beyond the pituitary. Proteolytic cleavage is a ... [more ▼]

Prolactin (PRL), originally associated with milk secretion, is now known to possess a wide variety of biological actions and diverse sites of production beyond the pituitary. Proteolytic cleavage is a common post-translational modification that can either activate precursor proteins or confer upon the peptide fragment unique biological actions not exerted by the parent molecule. Recent studies have demonstrated that the 16-kDa N-terminal proteolytic cleavage product of PRL (16K-PRL) acts as a potent inhibitor of angiogenesis. Despite previous demonstrations of 16K-PRL production in vivo, biological functions beyond its antiangiogenic actions remain unknown. Here we show that 16K-PRL, but not full-length PRL, acts to promote the expression of the inducible isoform of nitric oxide synthase (iNOS) and nitric oxide (*NO) production by pulmonary fibroblasts and alveolar type II cells with potency comparable with the proinflammatory cytokines interleukin-1beta, interferon gamma, and tumor necrosis factor alpha. The differential effect of 16K-PRL versus PRL occurs through a receptor distinct from known PRL receptors. Additionally, pulmonary fibroblasts express the PRL gene and endogenously produce 16K-PRL, suggesting that this pathway may serve both autocrine and paracrine roles in the regulation of *NO production. These results reveal that proteolytic cleavage of PRL confers upon this classical hormone potent iNOS inducing activity, suggesting its role in inflammatory/immune processes. [less ▲]

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