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See detailPregnancy-Associated Glycoprotein K-60 kDa [Fragment] from Bubalus bubalis placenta - Access number P83369
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-associated glycoprotein levels in cattle exhibiting prolonged pregnancy: case reports.
Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg

in Proceedings of the 6th European Congress of Bovine Health and Management (ECBHM) (2011, September 07)

Prolonged gestation (PG) is a complex clinical entity which occurs in bovine species. This pathology has been described in Belgian Blue cattle. PG may require termination by cesarean section. Fetuses ... [more ▼]

Prolonged gestation (PG) is a complex clinical entity which occurs in bovine species. This pathology has been described in Belgian Blue cattle. PG may require termination by cesarean section. Fetuses often exhibit increased body weight, long hair and well erupted teeth. All calves die within minutes or hours after birth. From endocrinological point of view, PG is highly correlated to fetal adenohypophyseal aplasia. The aim of the present study was to investigate the usefulness of PAG RIA for diagnosis of prolonged gestation in Belgian Blue cattle. Blood samples were collected from five Blue Belgian cows having an abnormally long pregnancy length (10 to 15 months). PAG was measured by routine RIA with the use of antiserum AS497 as primary antibody. A purified bovine PAG preparation (boPAG67kDa) was used as standard (0.8 to 100 ng/mL) and for radio-iodination (125I). Cesarean section was used for termination of pregnancy of those cows exhibiting abnormal PAG concentrations. Morphological aspect and viability of fetuses were reported. Concentrations of PAG were very low (94.1 ng/mL, 50 ng/mL, 5 ng/mL, 110 ng/mL and 26 ng/mL) in the five females suffering of prolonged gestation. Fetuses issued from prolonged gestation exhibited long hair, well erupted teeth and died within few minutes after birth. In conclusion, PAG concentration is a good indicator of prolonged gestation in cattle. Concentrations are much lower than those expected in normal ongoing pregnancy (<120 ng/mL versus >1,000 ng/mL). [less ▲]

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See detailPregnancy-associated glycoprotein levels in pregnant goats inoculated with Toxoplasma gondii or Listeria monocytogenes: a retrospective study
Zarrouk, A.; Engeland, I. V.; Sulon, Joseph ULg et al

in Theriogenology (1999), 52(6), 1095-104

The pregnancy-associated glycoprotein (PAG) concentration profiles of goats that had been experimentally inoculated with either Toxoplasma gondii or Listeria monocytogenes are described. All goats were ... [more ▼]

The pregnancy-associated glycoprotein (PAG) concentration profiles of goats that had been experimentally inoculated with either Toxoplasma gondii or Listeria monocytogenes are described. All goats were examined regularly by ultrasonography. In T. gondii-infected females (n = 5), a slow decrease of PAG was observed throughout a period of 55 to 74 d after inoculation. Afterwards, the goats either aborted (n = 4) or kidded 1 dead and 1 weak fetus (n = 1). In L. monocytogenes-infected females (n = 8), a marked decrease of PAG was observed from the day of inoculation. Abortion occurred within 9 to 11 d post inoculation (n = 7). Only 1 goat kidded a healthy fetus. [less ▲]

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See detailPregnancy-associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows.
El-Battawy, K. A.; Melo de Sousa, Noelita ULg; Szenci, Otto et al

in Reproduction in Domestic Animals (2009), 44(2), 161166

Pregnancy-associated glycoprotein (PAG) concentrations were measured in buffalo cows starting from day 28 after breeding. Oestrus was synchronized in 10 buffaloes using two injections of 25 mg ... [more ▼]

Pregnancy-associated glycoprotein (PAG) concentrations were measured in buffalo cows starting from day 28 after breeding. Oestrus was synchronized in 10 buffaloes using two injections of 25 mg prostraglandin (PG)F(2alpha) (Lutalyse((R))) at a 11-day interval. Blood sampling was conducted nearly twice weekly. Results indicated that plasma PAG concentrations in non-pregnant buffaloes were low (<0.20 ng/ml) during the whole experimental period (day 28 to 103), while in pregnant animals plasma PAG levels increased from day 28 (4.48 +/- 0.92 ng/ml) until day 41 (27.27 +/- 6.74 ng/ml), remaining high (20.71 +/- 9.20 ng/ml) until day 103. Progesterone levels were significantly (p < 0.0001) higher in pregnant (3.51-4.80 ng/ml) than in non-pregnant buffaloes (0.28-1.52 ng/ml). A significant difference (p < 0.0001) in plasma PAG concentrations between pregnant and non-pregnant animals starting at day 28 after breeding suggests that PAG-radioimmunoassay could be suitable for pregnancy diagnosis in buffaloes during this period. In conclusion, PAG test offers the advantages that it requires a single plasma sample for early pregnancy diagnosis as well as the accuracy of the test for the detection of pregnancy as early as day 28. [less ▲]

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See detailPregnancy-associated glycoprotein profiles of 5 heifers measured by three radioimmunoassay systems.
Perenyi, Z.; Desbuleux, H.; Sulon, J. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 6-7

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See detailPregnancy-associated glycoprotein secretion in North Moroccan goats
Chentouf, M.; El Amiri, B.; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2008), 43(6), 696-700

The plasma profile of pregnancy-associated glycoprotein (PAG) and its relationship with fetal number were studied in 14 North Moroccan goats using a semi-heterologous radioimmunoassay (RIA). Peripheral ... [more ▼]

The plasma profile of pregnancy-associated glycoprotein (PAG) and its relationship with fetal number were studied in 14 North Moroccan goats using a semi-heterologous radioimmunoassay (RIA). Peripheral blood was collected every day during the first month of pregnancy, afterward the blood samples were collected trice a week. The PAG were first detected at day 20 of pregnancy, their levels increase to week 8 of gestation then decrease slightly until parturition. Statistical differences between goats carrying one or two foetuses are observed from week 5 of pregnancy until parturition. Factorial Discriminant Analysis provides mathematical models for the discrimination between the litter size using the PAG level and the week of pregnancy. Using only one blood sample per week, high sensitivity, specificity and overall accuracy (74%, 88% and 81%) were obtained using these models. It is concluded that the PAG RIA is an effective tool for early diagnosis of pregnancy and for discrimination between the litter sizes in North Moroccan goats. [less ▲]

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See detailPregnancy-associated glycoprotein, chymosin and pepsinogen immunoreactivity of proteins extracted from fetal gastric tissue in bovine species.
Bella, Amina ULg; Melo de Sousa, Noelita ULg; Dehimi, M. L. et al

in Research in Veterinary Science (2012), 92(3), 378-386

The objective of this work was to investigate the expression of gastric aspartic proteinases in fundic and pyloric mucosa removed from bovine fetuses. For this purpose, fractions issued from classical ... [more ▼]

The objective of this work was to investigate the expression of gastric aspartic proteinases in fundic and pyloric mucosa removed from bovine fetuses. For this purpose, fractions issued from classical biochemical protocols were analyzed by proteolytic method, by PAG-RIA and by Western blot with the use of antisera raised against both pepsinogens and PAG. A strong reaction of proteins extracted from the fundic mucosa collected at the beginning of pregnancy was revealed with both anti-bPAG-I and anti-bPAG-II antisera, suggesting the expression of pepsinogen F in bovine species. Concerning pyloric mucosa, the analysis by Western blot highlighted a very strong immunoreaction with the anti-bovine chymosin serum. Amino-terminal sequencing allowed to identify bovine fetuin and albumin in fundic extracts, chymosin in the pyloric mucosa extracts, as well as some unknown proteins in both mucosa. Despite no N-terminal microsequence corresponding to the hypothetical pepsinogen F could be identified, it cannot be excluded that an existing bovine pepsinogen F-like molecule could be degraded during the purification procedure or that co-purified proteins could be responsible for masking its N-terminal microsequence. [less ▲]

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See detailPregnancy-Associated Glycoproteins (Pag) as Pregnancy Markers in the Ruminants
Melo de Sousa, Noelita ULg; Ayad, A.; Beckers, Jean-François ULg et al

in Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society (2006), 57(Suppl 8), 153-71

In the last years, a polymorphic family of placenta-expressed proteins has been discovered in ruminant species and used for pregnancy diagnosis. Pregnancy diagnosis is an important part in reproduction ... [more ▼]

In the last years, a polymorphic family of placenta-expressed proteins has been discovered in ruminant species and used for pregnancy diagnosis. Pregnancy diagnosis is an important part in reproduction management of ruminants. The pregnancy-associated glycoproteins (PAG) are synthesized in the mono- and binucleate cells of the ruminant's trophectoderm. Part of them is released into maternal blood circulation where they can be assayed by different RIA and ELISA systems. Due to large variety of expressed molecules and to large variations in the post-translational processing of the glycoproteins, different immuno-systems present different ability to quantify the PAG released in blood. Recent investigations showed that surprisingly the level of milk production in ruminants can modify the concentration of PAG circulating in blood. On the whole, the data show that the RIA methods are very precise for measuring PAG concentrations in the maternal blood and milk of the ruminants. Different studies clearly indicate that milk can be used for pregnancy diagnosis in small ruminants. The sensitivity and specificity of this method are very high. The results showed the possibility of the use PAG in milk and in blood as pregnancy test. It is especially helpful in the diagnosis of gestation and in detection of embryonic mortality as a non stressed method in the pregnancy management in the ruminants. [less ▲]

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See detailPregnancy-associated glycoproteins (PAG) concentrations in embryo recipient goats and mated goats
Calero, P; Gonzalez, F; Cabrera, F et al

in Proceeding of pregnancy-associated glycoproteins (PAG) concentrations in embryo recipient goats and mated goats (2002, September 06)

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See detailPregnancy-associated glycoproteins (PAG) in Bos taurus taurus and Bos taurus indicus.
El Amiri, B.; Melo de Sousa, Noelita ULg; Perenyi, Z. et al

in Theriogenology (2000), 53

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See detailPregnancy-associated glycoproteins (PAG) in postpartum cows, ewes, goats and their offspring
Haugejorden, G.; Dahl, E.; Karlberg, K. et al

in Proceedings of the 15th International Congress on Animal Reproduction (2004)

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See detailPregnancy-Associated Glycoproteins (PAGs) in prolific (Boujaâd x D’man) and non- prolific (Boujaâd) Moroccan sheep by means of four radio-immunoassays
El Amiri, Bouchra; Melo de Sousa, Noelita ULg; El Abbadi, N et al

in Proceedings of the 23rd International Congress of Hungarian Association for Buiatrics (2013)

The aim of the present study was: i) to investigate the use of PAGs for pregnancy follow up in Boujaâd non prolific (n=8) vs. Boujaâd x D’man a prolific sheep (n=20), ii) to study whether the boPAG tracer ... [more ▼]

The aim of the present study was: i) to investigate the use of PAGs for pregnancy follow up in Boujaâd non prolific (n=8) vs. Boujaâd x D’man a prolific sheep (n=20), ii) to study whether the boPAG tracer (daily available at the laboratory of physiology of reproduction, Liège, could be used in RIA without affecting the results, iii) to prospect the possibility of transferring the technique to a Moroccan laboratory. The blood samples were weekly collected from the first week of gestation till the 4 week after lambing. The RIA1, RIA2, RIA3 and RIA4 were all based on ovPAG standard, while the tracer and antiserum changed according to the system. The RIA1, RIA3 and RIA4 were performed in Belgium while the RIA2 was carried out in Morocco. The results showed that the highest correlation was obtained between the RIA3 and RIA4 (R2 = 0.83) while the lowest was recorded between the RIA2 and RIA4 (R2 = 0.69). The lowest concentrations derived from the RIA2. The highest concentrations were recorded before lambing (279.29; 88.21; 268.75; 152.67 ng/ml respectively for RIAs 1, 2, 3 and 4). To conclude, the profiles of ovPAG showed that it is possible to flow-up with success using the four systems. In addition, the RIA1 using boPAG as tracer could be a good alternative to the homologous one and finally, it is possible to set up routinely the RIA technique for PAG detection in Moroccan laboratories. [less ▲]

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See detailPregnancy-associated glycoproteins as diagnostic tool in cattle reproduction
Gajewski, Zdzislaw; Pertajitis; Melo de Sousa, Noelita ULg et al

in Schweizer Archiv für Tierheilkunde (2009), 151(12), 577-582

Pregnancy-associated glycoproteins are powerful pregnancy markers in domestic cattle. These proteins are expressed in mono- and binucleate trophoblast cells from the first days of gestation until calving ... [more ▼]

Pregnancy-associated glycoproteins are powerful pregnancy markers in domestic cattle. These proteins are expressed in mono- and binucleate trophoblast cells from the first days of gestation until calving. Different molecules were identified as being expressed at various stages of pregnancy. However, up to date, their functions and activities during pregnancy were not yet established. Specific RIA tests were developed (classic and alternative RIA) and used to measure the concentration of these glycoproteins in blood during gestation and the postpartum period in cattle. In maternal blood, PAGs rise to detectable levels from the 24th-28th days after fertilization. A recent study indicated that PAGs can also be detected in milk samples. However, concentrations in milk are much lower when compared to those of plasma. [less ▲]

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See detailPregnancy-associated glycoproteins in sheep: breeds, techniques and achievements
El Amiri, Bouchra; Melo de Sousa, Noelita ULg; Szenci, Otto et al

in Proceedings of the 23rd International Congress of Hungarian Association for Buiatrics (2013)

The aim of the present review is to summarize the achievements on pregnancy-associated glycoproteins in sheep during the last 20 years on terms of results obtained after purifications, molecular biology ... [more ▼]

The aim of the present review is to summarize the achievements on pregnancy-associated glycoproteins in sheep during the last 20 years on terms of results obtained after purifications, molecular biology, radio-immunoassays (RIA), enzyme linked immune sorbent assay (ELISA), double radial immune-diffusion (Ouchterlony) and Western blot techniques. The analysis of documented papers that concerned these attempts showed that the animal genetic material used was composed of variable breeds from north Africa, Europe and USA (the Aragonesa, Ouled Djellal, Laucaun drairy sheep, Berrichone, Texel x Norvegian crossed ewes, Awassi x Merino, Blackheaded Moutton sheep x Blackheaded German Mutton sheep (BGM), Rhoen sheep crosses of BGM with Dorper sheep, Assaf, Chura, Merino, Texel, Suffolk, Boujaâd Moroccan breed). The RIA represents the wildspread technique used (54%) while Oucherlony and Western blot had been limited in use (4% each). From 1990 to 2000 only 27% of the achievements being available today were realized. The main data, profiles and studies were performed from 2000 to now (63%). To conclude, this review allows summarizing and updating the knowledge about the PAG in sheep. [less ▲]

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See detailThe pregnancy-associated glycoproteins in sheep: new investigations by using the Ouchterlony technique.
El Amiri, B.; Garbayo, J. M.; Mecif, K. et al

in Proceedings of the 15th Scientific Meeting of the European Embryo Transfer Association (1999)

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See detailPregnancy-associated glycoproteins in the ruminants: inactive members of the Aspartic Protease family
Beckers, Jean-François ULg; Drion, Pierre ULg; Garbayo, J. M. et al

in Proceedings of the Middel European Buiatrics Congress, 1998 (1998, May 21)

Proteins secreted by the placenta, when detected in the peripheral circulation of the mother, can be useful indicators of both pregnancy and feto-trophoblast well-being (1-3). In 1982, Butler et al. (4 ... [more ▼]

Proteins secreted by the placenta, when detected in the peripheral circulation of the mother, can be useful indicators of both pregnancy and feto-trophoblast well-being (1-3). In 1982, Butler et al. (4) isolated two pregnancy-specific proteins (PSPA and PSPB) from bovine placental membranes. PSPA was identified as a-fetoprotein which is not strictly limited to pregnancy, while PSPB was confirmed as placenta’s and pregnancy’s specific (5). PSPB was characterized as a glycoprotein showing relative molecular masses (Mr) between 47 and 53 kDa and presenting different isoelectric points (from 4.0 to 4.4). The Mr of PSPB was similar to the Mr of the molecule isolated by Laster in 1977 (6). In 1991, Zoli et al. (7) purified a pregnancy-associated glycoprotein (PAG), later designated PAG_1 and presently designated as PAG_I_ 67 considering its Mr. When isolated from bovine fetal cotyledons PAG_I_67 was an acidic glycoprotein of 67 kDA. Four isoforms (PI : 4.4, 4.6, 5.2 and 5.4) were detected in the initial preparation. Later molecular cloning studies showed that the PSPB and PAG_I_67 were closely related in primary structure (8). These glycoproteins (either PSPB and PAG_I_67) could be detected in the maternal circulation at around the time that the trophoblast formed definitive attachment to the uterine wall (figures A & B). Concentrations increased gradually therefore and reached peak values just before parturition at about 1 to 5 mg/ml (9).The PSPB and PAG molecules are routinely determined in peripheral maternal blood as pregnancy markers in cattle (5, 9,10). Glycoproteins immunologically related to PAG_I_67 and PSPB have been isolated and partially characterized from ovine fetal cotyledons: oPAG later designated oPAG_I (11) and oPSPB (12). They also have been detected in maternal blood by week third (12) or fourth (13) after breeding. Different forms (differing in Mr and isoelectric point) were characterized after isolation from sheep cotyledons cultured in vitro (14). Very recently, 3 different PAGs were characterized from goat placenta having Mr of 55, 59 and 62 kDa. Each of them presented various isoeletric points (15). In 1991, Xie et al., cloned PAG (now known as PAG_I_67) from late bovine and ovine placenta by screening cDNA libraries with two anti-PAG antisera (16). The bovine and ovine cDNAs encoding PAG_I shared 86% nucleotide sequence identic with one and other and encoded proteins of 380 and 382 aminoacids respectively including a 15 aminoacid signal sequence. However, protein sequence data, (peptidic sequencing) already showed the first aminoacid of the bovine PAG_I_67 was an arginine that corresponded to another one located at position 39, downstream of the side of signal sequence cleavage, indicating that PAG_I_67 undergoes post-translational modifications from a proform. [less ▲]

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See detailPregnancy-associated glycoproteins isolated from ewe placenta
El Amiri, B; Remy, B; Melo de Sousa, Noelita ULg et al

in Proceeding of pregnancy-associated glycoproteins isolated from ewe placenta (2002, September 06)

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See detailPregnancy-associated glycoproteins isolated from the ewe placenta removed at two different stages of gestation.
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

in Proceedings of the 7e Journée de Rencontre Bioforum (2002)

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