Progression in MCF-7 Breast Cancer Cell Tumorigenicity: Compared Effect of FGF-3 and FGF-4.
; Deroanne, Christophe ; Noël, Agnès et al
in Breast Cancer Research and Treatment (2000), 60(1), 15-28
The transforming properties of fibroblast growth factor 3 (FGF-3) were investigated in MCF7 breast cancer cells and compared to those of FGF-4, a known oncogenic product. The short form of fgf-3 and the ... [more ▼]
The transforming properties of fibroblast growth factor 3 (FGF-3) were investigated in MCF7 breast cancer cells and compared to those of FGF-4, a known oncogenic product. The short form of fgf-3 and the fgf-4 sequences were each introduced with retroviral vectors and the proteins were only detected in the cytoplasm of the infected cells, as expected. In vitro, cells producing FGF-3 (MCF7.fgf-3) and FGF-4 (MCF7.fgf-4) displayed an amount of estrogen receptors decreased to around 45% of the control value. However, MCF7.fgf-3 cell proliferation remained responsive to estradiol supply. The sensitivity of the MCF7.fgf-4 cells, if existant, was masked by the important mitogenic action exerted by FGF-4. In vivo, the MCF7.fgf-3 and MCF7.fgf-4 cells gave rise to tumors under conditions in which the control cells were not tumorigenic. Supplementing the mice with estrogen had the paradoxical effect of totally suppressing the start of the FGF-3 as well as the FGF-4 tumors. Tumorigenicity in the presence of matrigel was similar for MCF7.fgf-3 and control cells and was increased by estrogen supplementation. Once started, the MCF7.fgf-4 tumors grew with a characteristic high rate. Remarkably, FGF-4 but not FGF-3, stimulated the secretion of vascular endothelial growth factor (VEGF165) without altering the steady-state level of its mRNA, suggesting a possible regulation of VEGF synthesis at the translational level in MCF7 cells. The increased VEGF secretion is probably involved in the more aggressive phenotype of the MCF7.fgf-4 cells while a decreased dependence upon micro-environmental factors might be part of the increased tumorigenic potential of the MCF7.fgf-3 cells. [less ▲]Detailed reference viewed: 35 (3 ULg)
Progression micrograduée dans l'entraînement à la vision dans l'espace et à la description anatomique
Bonnet, Pierre ; Reggers, Thérèse
in Revue Internationale des Technologies en Pédagogie Universitaire (2006), 3(1-2),
Anatomy teaching for first year medical sciences students in Liege Medical School, Belgium, s based on both lectures and e-learning. An introductive lesson on anatomical description has been developed on ... [more ▼]
Anatomy teaching for first year medical sciences students in Liege Medical School, Belgium, s based on both lectures and e-learning. An introductive lesson on anatomical description has been developed on e-learning as a tool for description and spatial view training. That first on-line lesson has been constructed by identifying the conceptual requisite to understand anatomy and its specific descriptive language, by dissection and finally by organisation of those requisites to allow generalisation and transfer. The lesson conception gives the students the opportunity to practice the exercise at their own rhythm trough all the steps of the reflective process. That e-learning lesson has been regulated by a follow-up of two years practice in real conditions. This article emphasizes the methodological principles which are the base of the micro graduated structure of the distance course. [less ▲]Detailed reference viewed: 23 (10 ULg)
Progression model tissue microarray (TMA) for the study of uterine carcinomas.
; Boniver, Jacques ; Delvenne, Philippe
in Disease Markers (2010), 28(5), 267-72
Cervical and endometrial uterine carcinomas are heterogeneous groups of cancers, which are preceded by preneoplastic lesions. More accurate tools are needed to improve the diagnosis and to define markers ... [more ▼]
Cervical and endometrial uterine carcinomas are heterogeneous groups of cancers, which are preceded by preneoplastic lesions. More accurate tools are needed to improve the diagnosis and to define markers which may be relevant for the diagnosis, prediction of disease progression and therapeutic response.High throughput technologies for testing and validating molecular targets in cancer lesions and in their precursors are presently available. Among them, the tissue microarray (TMA) presents the advantage of a morphological control of the analyzed tissue fragment. In this article, we review the different aspects of the TMA technology with a special consideration to a uterine carcinogenesis model. [less ▲]Detailed reference viewed: 11 (3 ULg)
Progression of the prevalence of metabolic syndrome in normal-weight US subjects.
in Archives of Internal Medicine (2009), 169(6), 636-637Detailed reference viewed: 29 (4 ULg)
Progressive bone density gains with once-monthly oral ibandronate: the MOBILE study long-term extension
; Reginster, Jean-Yves ; et al
in Arthritis and Rheumatism (2006, November), 54Detailed reference viewed: 14 (8 ULg)
Progressive Eddy Current modeling via a finite element subproblem method
Dular, Patrick ; ; et al
in International Journal of Applied Electromagnetics and Mechanics (2013)Detailed reference viewed: 7 (0 ULg)
Progressive encephalopathy in a child with cerebral folate deficiency syndrome.
; RAMAEKERS, Vincent ; et al
in Journal of Child Neurology (2008), 23(12), 1460-3
Cerebral folate deficiency syndrome, a recently recognized cause of developmental delay, regression, and seizures, is associated with autoantibodies against folate receptors. A female child with ... [more ▼]
Cerebral folate deficiency syndrome, a recently recognized cause of developmental delay, regression, and seizures, is associated with autoantibodies against folate receptors. A female child with developmental delay and a history of seizures who presented with seizures and unexplained coma is reported. Extensive testing to evaluate the patient's coma and subsequent developmental regression were unrevealing until the results of her cerebrospinal fluid neurotransmitter analysis returned. These showed low levels of methyltetrahydrofolate, the active metabolite of folate in the cerebrospinal fluid; subsequently, elevated titers of autoantibodies against folate receptors were found. Despite treatment with folinic acid, she developed intractable epilepsy and severe developmental delay. [less ▲]Detailed reference viewed: 13 (1 ULg)
Progressive fixed-ratio schedule reveals long-lasting motivation deficit after transient cerebral ischemia in mice.
Linden, Jérôme ; Plumier, Jean-Christophe ; Ferrara, André et al
Poster (2010, November)Detailed reference viewed: 43 (20 ULg)
Progressive juvenile glomerulonephropathy in 16 related French Mastiff (Bordeaux) Dogs
Lavoué, Rachel ; ; et al
in Journal of Veterinary Internal Medicine (2010), 24(2), 314-322Detailed reference viewed: 28 (7 ULg)
Progressive load redistribution in continuous concrete beams and slabs under fire conditions
Dotreppe, Jean-Claude ; ; Franssen, Jean-Marc
in Proceedings of the fib Symposium "Keep concrete attractive" Vol.2 (2005)Detailed reference viewed: 20 (3 ULg)
The prohibition of dogcarts in Belgium: a hidden agricultural policy?
in Moser, Peter; Varley, Tony (Eds.) Integration through Subordination. The politics of Agricultural Modernisation in Industrial Europe (2013)Detailed reference viewed: 35 (10 ULg)
Proinflammatory Cytokines Induce Bronchial Hyperplasia and Squamous Metaplasia in Smokers: Implications for chronic obstructive pulmonary disease therapy.
Herfs, Michael ; Hubert, Pascale ; POIRRIER, Anne-Lise et al
in American Journal of Respiratory Cell and Molecular Biology (2012), 47(1), 67-79
Tracheobronchial squamous metaplasia is common in smokers and is associated with both airway obstruction in chronic obstructive pulmonary disease (COPD) and increased risk of lung cancer. Whereas this ... [more ▼]
Tracheobronchial squamous metaplasia is common in smokers and is associated with both airway obstruction in chronic obstructive pulmonary disease (COPD) and increased risk of lung cancer. Whereas this reversible epithelial replacement is almost always observed in association with chronic inflammation, the role of inflammatory mediators in the pathogenesis of squamous metaplasia is still unclear. In the present study, we investigated the implication of cigarette smoke-mediated pro-inflammatory cytokine up-regulation in the development and treatment of tracheobronchial epithelial hyperplasia and squamous metaplasia. By using immunohistological techniques, we showed a higher epithelial expression of TNFalpha, IL-1beta and IL-6 as well as an activation of NF-kappaB and AP-1/MAPK signalling pathways in the respiratory tract of smoking patients compared to the normal ciliated epithelium of non-smoking patients. In addition, we demonstrated that these signalling pathways strongly influence the proliferation and the differentiation state of in vitro generated normal human airway epithelial basal cells. Finally, we exposed mice to cigarette smoke for 16 weeks and demonstrated that anti-TNFalpha (etanercept), anti-IL-1beta (anakinra) and/or anti-IL-6R (tocilizumab) therapies significantly reduced epithelial hyperplasia and squamous metaplasia development. These data highlight the importance of soluble inflammatory mediators in the pathogenesis of tracheobronchial squamous metaplasia. Therefore, administration of pro-inflammatory cytokine antagonists may have clinical application in the management of COPD patients. [less ▲]Detailed reference viewed: 66 (21 ULg)
A proinflammatory role for the cyclopentenone prostaglandins at low micromolar concentrations: Oxidative stress-induced extracellular signal-regulated kinase activation without NF-kappa B inhibition
Bureau, Fabrice ; Desmet, Christophe ; et al
in Journal of Immunology (2002), 168(10), 5318-5325
An anti-inflammatory role and therapeutic potential for cyclopentenone PGs (cyPGs) has been suggested, based on observations that levels of cyPGs in exudates increase during the resolution phase of ... [more ▼]
An anti-inflammatory role and therapeutic potential for cyclopentenone PGs (cyPGs) has been suggested, based on observations that levels of cyPGs in exudates increase during the resolution phase of inflammation, and that exogenous cyPGs may attenuate the inflammatory response in vivo and in vitro mainly through inhibition of NF-kappaB, a critical activator of inflammatory gene expression. However, exogenous cyPGs inhibit NF-kappaB only at concentrations substantially higher than those of endogenous cyPGs present in inflammatory fluids, thus challenging the hypothesis that cyPGs are naturally occurring inhibitors of inflammation and suggesting that cyPGs at low concentrations might have previously unappreciated effects. In this study, using various cell types, we report that cyPGs, when used at concentrations substantially lower than required for NF-kappaB inhibition (viz, low micromolar concentrations), significantly potentiate the inflammatory response to TNF-alpha. At these concentrations, cyPGs induce production of reactive oxygen species, thereby synergizing with TNF-alpha to activate the extracellular signal-regulated kinase 1/2, an activation which in turn potentiates proinflammatory cytokine expression at both transcriptional and posttranscriptional levels. Our studs establishes a proinflammatory role for cyPGs at low micromolar concentrations, raises the possibility that cyPGs do not act as physiologic anti-inflammatory mediators, and questions the therapeutic potential of these compounds. [less ▲]Detailed reference viewed: 36 (9 ULg)
Project FP7 OMNISCIENTIS for Odour Monitoring Applications in Industrial Plants and Communities
Conference (2013, October 03)Detailed reference viewed: 23 (1 ULg)
The project of 25 low-cost and low energy houses in Marchin (Belgium)
Dupagne, Albert ; Hauglustaine, Jean-Marie
Conference (1983, November)
A first research funded by the Commission of the European Communities has led to a manual design methodology, mainly orientated to the conception of low-cost houses, integrating the fundamental thermal ... [more ▼]
A first research funded by the Commission of the European Communities has led to a manual design methodology, mainly orientated to the conception of low-cost houses, integrating the fundamental thermal concepts into the design process, without any use of sophisticated technology. The present paper concerns a practical application of this design methodology, used for a project of 25 low-energy houses designed, built and to be monitored. From the first design step until the detailed design, the research team has followed the objective to obtain houses having estimated heating energy needs 50 % lower than commonly, without any investment overrun, even on a difficult North sloped site. The design methodology has been an optimization tool of the volume, the plan, the North and South fenestration, the insulating material, etc. The construction elements were usual (for cost reasons), but their installation has been improved in a manner which allows a continuous control during the construction, giving no the guarantee to obtain thermal properties as close as possible to the expected ones. A detailed monitoring will measure the actual heating energy consumption and thus check the first evaluations. [less ▲]Detailed reference viewed: 34 (2 ULg)