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See detailPrimary Structure of Selected Archaeal Mesophilic and Extremely Thermophilic Outer Surface Layer Proteins
Claus, Harald; Akça, Erol; Debaerdemaeker, Tony et al

in Systematic & Applied Microbiology (2002), 25

The archaea are recognized as a separate third domain of life together with the bacteria and eucarya. The archaea include the methanogens, extreme halophiles, thermoplasmas, sulfate reducers and sulfur ... [more ▼]

The archaea are recognized as a separate third domain of life together with the bacteria and eucarya. The archaea include the methanogens, extreme halophiles, thermoplasmas, sulfate reducers and sulfur metabolizing thermophiles, which thrive in different habitats such as anaerobic niches, salt lakes, and marine hydrothermals systems and continental solfataras. Many of these habitats represent extreme environments in respect to temperature, osmotic pressure and pH-values and remind on the conditions of the early earth. The cell envelope structures were one of the first biochemical characteristics of archaea studied in detail. The most common archaeal cell envelope is composed of a single crystalline protein or glycoprotein surface layer (S-layer), which is associated with the outside of the cytoplasmic membrane. The S-layers are directly exposed to the extreme environment and can not be stabilized by cellular components. Therefore, from comparative studies of mesophilic and extremely thermophilic S-layer proteins hints can be obtained about the molecular mechanisms of protein stabilization at high temperatures. First crystallization experiments of surface layer proteins under microgravity conditions were successful. Here, we report on the biochemical features of selected mesophilic and extremely archaeal S-layer (glyco-) proteins. [less ▲]

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See detailPrimary structure of the Streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces lividans and nucleotide sequence of the gene
Duez, Colette ULg; Fraipont, Claudine ULg; Joris, Bernard ULg et al

in European Journal of Biochemistry (1987), 162

An 11450-base DNA fragment containing the gene for the extracellular active-site serine DD-peptidase of Streptomyces R61 was cloned in Streptomyces lividans using the high-copy-number plasmid pIJ702 as ... [more ▼]

An 11450-base DNA fragment containing the gene for the extracellular active-site serine DD-peptidase of Streptomyces R61 was cloned in Streptomyces lividans using the high-copy-number plasmid pIJ702 as vector. Amplified expression of the excreted enzyme was observed. Producing clones were identified with the help of a specific antiserum directed against the pure DD-peptidase. The coding sequence of the gene was then located by hybridization with a specific nucleotide probe and sub-fragments were obtained from which the nucleotide sequence of the structural gene and the putative promoter and terminator regions were determined. The sequence suggests that the gene codes for a 406-amino-acid protein precursor. When compared with the excreted, mature DD-peptidase, this precursor possesses a cleavable 31-amino-acid N-terminal extension which has the characteristics of a signal peptide, and a cleavable 26-amino-acid C-terminal extension. On the basis of the data of Joris et al. (following paper in this journal), the open reading frame coding for the synthesis of the DD-peptidase was established. Comparison of the primary structure of the Streptomyces R61 DD-peptidase with those of several active-site serine β-lactamases and penicillin-binding proteins of Escherichia coli shows homology in those sequences that comprise the active-site serine residue. When the comparison is broadened to the complete amino acid sequences, significant homology is observed only for the pair Streptomyces R61 DD-peptidase/Escherichia coli ampC β-lactamase (class C). Since the Streptomyces R61 DD-peptidase and β-lactamases of class A have very similar three-dimensional structures [Kelly et al. (1986) Science (Wash. DC) 231, 1429–1431; Samraoui et al. (1986) Nature (Lond.) 320, 378–380], it is concluded that these tertiary features are probably also shared by the β-lactamases of class C, i.e. that the Streptomyces R61 DD-peptidase and the β-lactamases of classes A and C are related in an evolutionary sense. [less ▲]

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See detailPrimary structure of the Streptomyces R61 extracellular DD-peptidase. 2. Amino acid sequence data
Joris, Bernard ULg; Jacques, Philippe; Frère, Jean-Marie ULg et al

in European Journal of Biochemistry (1987), 162(3), 519-524

In order to confirm the Streptomyces codon usage, the Streptomyces R61 DD-peptidase was fragmented by cyanogen bromide cleavage of the carboxymethylated protein, trypsin digestion of the carboxymethylated ... [more ▼]

In order to confirm the Streptomyces codon usage, the Streptomyces R61 DD-peptidase was fragmented by cyanogen bromide cleavage of the carboxymethylated protein, trypsin digestion of the carboxymethylated protein and trypsin digestion of the protein treated with beta-iodopenicillinate and endoxo-delta 4-tetrahydrophthalic acid. The isolated peptides, which altogether represented more than 50% of the polypeptide chain, were sequenced. The data thus obtained were in excellent agreement with the primary structure of the protein as deduced from the nucleotide sequence of the cloned gene. Though a weak acylating agent, beta-iodopenicillanate reacted selectively with the active site of the DD-peptidase and formed an adduct which mas much more stable than that formed with benzylpenicillin, thus facilitating the isolation and characterization of the active-site peptide. [less ▲]

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See detailPrimary structure of the wall peptidoglycan of leprosy-derived corynebacteria
Janczura, E.; Leyh-Bouille, Mélina; Cocito, C. et al

in Journal of Bacteriology (1981), 145(2), 775-779

The cell walls isolated from axenically grown leprosy-derived corynebacteria were submitted to various chemical and enzymatic degradations. The glycan strands of the wall peptidoglycan are essentially ... [more ▼]

The cell walls isolated from axenically grown leprosy-derived corynebacteria were submitted to various chemical and enzymatic degradations. The glycan strands of the wall peptidoglycan are essentially composed of N-acetylglycosaminyl-N-acetylmuramic acid disaccharide units. Small amounts of N-acetylglycosaminyl-N-glycolylmuramic acid (less than 10%) were also detected. The muramic acid residues of adjacent glycan strands are substituted by amidated tetrapeptide units which, in turn, are cross-linked through direct linkages extending between the C-terminal D-alanine residue of one tetrapeptide and the mesodiaminopimelic acid residue of another tetrapeptide. Such a structure is very similar to that of the wall peptidoglycan found in the taxonomically related microorganisms of the Corynebacterium, Mycobacterium, and Nocardia groups. [less ▲]

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See detailPrimary Systemic Amyloidosis - A report of 2 cases
Dezfoulian, Bita ULg; Arrese Estrada, Jorge ULg; Fernandez, A. et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (1996), 192

We report 2 cases of primary systemic amyloidosis. A monoclonal gammopathy was confirmed at the postmortem examination of the first patient. An extensive search for evidence of chronic infection ... [more ▼]

We report 2 cases of primary systemic amyloidosis. A monoclonal gammopathy was confirmed at the postmortem examination of the first patient. An extensive search for evidence of chronic infection, inflammation, neoplasms and paraproteinemia was conclusively negative in the other patient. The recognition of cutaneous signs of primary systemic amyloidosis is crucial to insure a rapid management aimed at postponing the fatal issue of the disease. [less ▲]

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See detailPrimary temporal bone angiosarcoma: a case report.
Scholsem, Martin; Raket, Daniel; Flandroy, Pierre et al

in Journal of neuro-oncology (2005), 75(2), 121-5

We present a rare case of temporal bone angiosarcoma diagnosed in a 26-year-old female patient at 36 week of pregnancy. The patient was referred with a 2 months history of left otalgia and tinnitus with a ... [more ▼]

We present a rare case of temporal bone angiosarcoma diagnosed in a 26-year-old female patient at 36 week of pregnancy. The patient was referred with a 2 months history of left otalgia and tinnitus with a tender swelling above the mastoid. Cranial imaging studies showed a 7 x 5 x 4 cm hypervascularized mass located in the left middle fossa with lysis of the temporal bone and extension to the subcutis. After the baby was delivered by caesarean section, the patient entered the oncology protocol. Selective embolization of the feeding vessels was followed by gross total surgical resection using a combined supra- and infra-tentorial approach. Pathological findings were those of a poorly differentiated, highly malignant sarcoma with a large epitheloid component and immunohistochemical evidence of endothelial differentiation (CD31, Factor VIII related antigen, CD34), consistent with an angiosarcoma with epitheloid features. No extra-cranial tumor was found after extensive staging. The patient received adjuvant radiotherapy followed by a course of chemotherapy consisting of 6 cycles of paclitaxel. At 15 months follow-up, she developed multiple distant metastasis to a left postauricular lymph node and to the lungs and ribs. The patient was given a second line chemotherapy using doxorubicine and ifosfamide. Despite an initial good response, she died with metastatic disease 26 months after diagnosis. We present a rare case of primary temporal bone angiosarcoma and report our experience with a multimode therapeutic approach combining surgery, radiotherapy and chemotherapy. [less ▲]

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See detailPrimary Versus Secondary Failure After Varicella Vaccination: Implications for Interval Between 2 Doses
Bonanni, Paolo; Gershon, Anne; Gershon, Michael et al

in Pediatric Infectious Disease Journal (2013), 32(7), 305-313

Background: Two-dose varicella vaccination is recommended for optimal control of varicella in populations with high (>90%) 1-dose coverage. Optimal timing of the second dose may depend on whether ... [more ▼]

Background: Two-dose varicella vaccination is recommended for optimal control of varicella in populations with high (>90%) 1-dose coverage. Optimal timing of the second dose may depend on whether breakthrough varicella results from primary vaccine failure (no protective immunity after vaccination) or secondary vaccine failure (waning protective immunity). Methods: Published literature (1995 to 2012) on vaccine failure after varicella vaccination cited in PubMed and other online sources was reviewed. Results: Nineteen publications detailed 21 varicella outbreaks with breakthrough varicella rates ranging from 0% to 42%; the publications showed no consistent trend between breakthrough varicella rate and time since vaccination. Conclusions: Literature to date indicates a relatively high rate of primary vaccine failure and limited evidence of secondary vaccine failure among 1-dose varicella vaccine recipients, suggesting that a short interval between 2 doses might be preferable in countries considering implementation of universal varicella vaccination to reduce breakthrough varicella. However, any potential disruption to well-established vaccination schedules should be considered. [less ▲]

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See detailPrimary versus secondary failure following varicella vaccination: implications for interval between two doses-Literature review
Bonanni, Paolo; Gershon, Anne; Gershon, Michael et al

Poster (2010)

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See detailLe primat de l’imaginaire sur le symbolique dans l’anthropologie de Maurice Godelier
Melice, Anne ULg

in Denooz, Joseph; Dortu, Véronique; Steinmetz, Rudy (Eds.) Mosaïques. Hommages à Pierre Somville (2007)

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See detailPrimate Behaviour and Parasite Transmission in the Ecuadorian Amazon
Martin, Sarah ULg; Carrillo Bilbao, Gabriel Alberto; Bernstein, Sophie et al

in Folia Primatologica : International Journal of Primatology = Internationale Zeitschrift für Primatologie = Journal international de Primatologie (2013, August 10), 84(3-5), 135-346

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See detailPrimates ecuatorianos en peligro por las actividades antropogénicas
Carrillo Bilbao, Gabriel Alberto; Martin, Sarah ULg; Huynen, Marie-Claude ULg

in Vanden Berghe, Kristine (Ed.) Amazonía: Civilisations et barbaries (2009)

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See detailLes primates
Vermeulen, Cédric ULg

in Delvingt, W.; Vermeulen, Cédric (Eds.) Nazinga (2007)

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See detailLa primauté de l'intentionnalité et ses conséquences épistémologiques
Boccaccini, Federico ULg

in Bulletin d'Analyse Phénoménologique (2014)

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See detailLa primauté de l’intentionnalité et ses conséquences philosophiques
Boccaccini, Federico ULg

Conference (2012, March)

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See detailLa primauté du concept
Duchesne, Jean-Patrick ULg

in Stiennon, Jacques; Duchesne, Jean-Patrick; Randaxhe, Yves (Eds.) Cinq siècles de peinture en Wallonie (1988)

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See detailPriming production of people's names
Valentine, T.; Moore, V.; Brédart, Serge ULg

in Quarterly Journal of Experimental Psychology (1995), 48A

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See detailLes Primitifs flamands
Allart, Dominique ULg

Conference given outside the academic context (2009)

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See detailPrimitive malignant rhabdoid tumor of the central nervous system in an adolescent. A case study
Eap, C; Litré, Claude Fabien ULg; Noudel, R et al

in Neuro-Chirurgie (2010), 56(5), 404-7

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See detailPrimitivisme agrarien ou capitalisme ? Economie et société chez les jansénistes du Grand Siècle
Vanhoorne, Frédérick ULg

in Cottret, Bernard; Cottret, Monique; Michel, Marie-José (Eds.) Jansénisme et puritanisme. Actes du colloque de Port-Royal des Champs, 14 septembre 2001 (2002)

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