Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailThe presence of a type IV collagen skeleton associated with periductal elastosis in breast cancer
Verhoeven, D.; Bourgeois, N.; Noël, Agnès ULg et al

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1990), 38

Using serial sections of frozen and AFA-fixed tissues from 34 breast cancers, we studied the presence of basement membrane material in the areas of elastosis. Various amounts of type IV collagen but not ... [more ▼]

Using serial sections of frozen and AFA-fixed tissues from 34 breast cancers, we studied the presence of basement membrane material in the areas of elastosis. Various amounts of type IV collagen but not of laminin were demonstrated in areas of periductal elastosis. In some tumors, type IV collagen accumulated beneath the basement membrane. Periductal elastosis in areas of extensive fibrosis showed focal type IV collagen immunoreactivity, indicating remnants of ducts. Interstitial elastosis corresponded with weak type IV collagen reactivity. Each tumor showed type IV collagen immunostaining of the elastotic areas, with various degrees of intensity. Negative crossreactivity of the type IV collagen antibody with elastin was verified in skin biopsies with solar elastosis. Pre-incubation of the antibody with large amounts of elastin demonstrated an identical immunoreactivity. The specificity of the antibody was confirmed by ELISA and by Western blot analysis. To explain the periductal elastosis, we propose the following hypothesis. Excessive production of basement membrane material by the epithelial cells of the ducts leads to formation of a type IV collagen skeleton. This skeleton can act as the matrix for a secondary deposition of elastic material. [less ▲]

Detailed reference viewed: 5 (0 ULg)
See detailPresence of alpha and beta estrogen receptors in human gingiva
Fraikin, N.; Munaut, Carine ULg; Lambert, Vincent ULg et al

in Journal of Dental Research (2002, December), 81(Sp. Iss. B), 239-239

Detailed reference viewed: 28 (6 ULg)
Full Text
Peer Reviewed
See detailPresence of aromatase and estrogen receptor alpha in the inner ear of zebra finches.
Noirot, Isabelle ULg; Adler, Henry J; Cornil, Charlotte ULg et al

in Hearing Research (2009)

Sex differences in song behavior and in the neural system controlling song in songbirds are well documented but relatively little is known about sex differences in hearing. We recently demonstrated the ... [more ▼]

Sex differences in song behavior and in the neural system controlling song in songbirds are well documented but relatively little is known about sex differences in hearing. We recently demonstrated the existence of sex differences in auditory brainstem responses in a songbird species, the zebra finch (Taeniopygia guttata). Many sex differences are regulated by sex steroid hormone action either during ontogeny or in adulthood. As a first step to test the possible implication of sex steroids in the control of sex differences in the zebra finch auditory system, we evaluated via immunocytochemistry whether estrogens are produced and act in the zebra finch inner ear. Specifically we examined the distribution of aromatase, the enzyme converting testosterone into an estrogen, and of estrogen receptors of the alpha subtype (ERalpha) in adult zebra finch inner ears. The anatomy of the basilar papillae was visualized by fluorescein-phalloidin, which delineated the actin structure of hair cells and supporting cells at their apical surface. Whole mount preparations of basilar papillae stained by immunocytochemistry revealed in both males and females an abundant aromatase distribution in the cytoplasm of hair cells, while ERalpha was identified in the nuclei of hair cells and of underlying supporting cells. Double labeled preparations confirmed the extensive co-localization of aromatase and ERalpha in the vast majority of the hair cells. These results are consistent with studies on non-avian species, suggesting a role for estrogens in auditory function. These findings are also consistent with the notion that estrogens may contribute to a sex difference in hearing. To our knowledge, this is the first demonstration of the presence of aromatase and of the co-localization of aromatase and ERalpha in the sensory epithelium of the inner ear in any animal model. [less ▲]

Detailed reference viewed: 75 (11 ULg)
Full Text
Peer Reviewed
See detailThe presence of bluetongue virus serotype 8 RNA in Belgian cattle since 2008.
Garigliany, Mutien-Marie ULg; Desmecht, Daniel ULg; De Clercq, Kris

in Transboundary and Emerging Diseases (2011), 58(6), 503-509

After a short winter break, bluetongue virus serotype 8 was responsible in 2007 for a large-scale epidemic among ruminant populations in Western Europe. Little is known about the mechanisms allowing the ... [more ▼]

After a short winter break, bluetongue virus serotype 8 was responsible in 2007 for a large-scale epidemic among ruminant populations in Western Europe. Little is known about the mechanisms allowing the virus to survive winter conditions. A yearly mass vaccination of cattle and sheep started in spring 2008, which was recognized as successful in terms of clinical protection, but occult circulation of the bluetongue virus has not been adequately addressed. We studied the carriage of bluetongue RNA in the spleen of cattle in the vector-free period and the circulation of bluetongue virus in cattle populations in Belgium since the introduction of vaccination programmes. Overall, the results presented here show evidence for the long-term carriage of bluetongue virus RNA in the spleen of cattle and demonstrated a low but significant circulation and transplacental transmission of bluetongue virus in Belgian cattle in 2009, with apparent disappearance in 2010. [less ▲]

Detailed reference viewed: 14 (1 ULg)
Full Text
Peer Reviewed
See detailPresence of Clostridium difficile in pigs and cattle intestinal contents and carcass contamination at the slaughterhouse in Belgium.
Rodriguez Diaz, Cristina ULg; Avesani, V.; Van Broeck, J. et al

in International Journal of Food Microbiology (2013), 166(2), 256-262

The objective of this study was to evaluate the presence of Clostridium difficile in intestinal and carcass samples collected from pigs and cattle at a single slaughterhouse. C. difficile was isolated in ... [more ▼]

The objective of this study was to evaluate the presence of Clostridium difficile in intestinal and carcass samples collected from pigs and cattle at a single slaughterhouse. C. difficile was isolated in 1% and 9.9% of the pig and cattle intestinal contents and in 7.9% and 7% of cattle and pig carcass samples respectively. A total of 19 different PCR-ribotypes were identified, among them types 078 and 014. Seven of 19 ribotypes correlated with the PCR-ribotypes involved in human C. difficile infections in Belgium. This study confirms that animals are carriers of C. difficile at slaughter and ribotypes are identical than those in humans, and that carcass contamination occurs inside the slaughterhouse. [less ▲]

Detailed reference viewed: 69 (8 ULg)
Full Text
Peer Reviewed
See detailPRESENCE OF CU-PHYCOERYTHRIN IN THE MARINE BENTHIC BLUE-GREEN-ALGA OSCILLATORIA CF CORALLINAE
Hoffmann, Lucien; Talarico, L.; Wilmotte, Annick ULg

in Phycologia (1990), 29

The presence of CU-phycoerythrin, a phycobiliprotein characterized by the presence of phycourobilin chromophores in addition to phycoerythrobilins, and so far found in only eight blue-green algae, is ... [more ▼]

The presence of CU-phycoerythrin, a phycobiliprotein characterized by the presence of phycourobilin chromophores in addition to phycoerythrobilins, and so far found in only eight blue-green algae, is reported for the first time from a marine benthic blue-green alga, Oscillatoria cf. corallinae. [less ▲]

Detailed reference viewed: 12 (0 ULg)
Full Text
Peer Reviewed
See detailPresence of gd TcR+ Tumor Infiltrating Lymphocytes (TILs) in One Case of Pediatric Osteogenic Sarcoma
Moutschen, Michel ULg; Jacobs, Nathalie ULg; Hoyoux, Claire ULg et al

in International Journal of Pediatric Hematology/Oncology (1994)

To characterize ab and gd TcR expression in cultured TILs from a metastatic osteogenic sar- coma.Patient and methods: TILs were isolated from a lung metastasis of an osteogenic sarcoma in a 16- year-old ... [more ▼]

To characterize ab and gd TcR expression in cultured TILs from a metastatic osteogenic sar- coma.Patient and methods: TILs were isolated from a lung metastasis of an osteogenic sarcoma in a 16- year-old female patient. Culture conditions were IL- 2 and anti-CD3 MoAb+IL-2. TcR expression and surface phenotype were studied by flow cytometry. 4h-chromium release assays were used to assess cytotoxicity. Results:IL-2-expanded tumor infiltrating T-lym- phocytes contained 50% gd TcR+ cells whereas gd TcR+ cells represented 20% of T-cells in cultures expanded with anti-CD3 MoAb+IL-2. The phenotype of gd TcR+ cells was CD3+, CD16-, CD4-, CD8-, and CD29+. AnH-CD3 MoAb + IL-2-expanded TILs mediated a significant lysis of thé autologous tumor and of an allogeneic lymphoma and displayed a higher activity against thé NK-sensitive target K562. In cold target experiments, unlabeled autol- ogous tumor cells blocked thé lysis of K562, indicating that MHC-unrestricted effectors were involved in thé lysis of both targets. Conclusions: This report demonstrates thé ex- pansion of large numbers of gd TcR+ T-cells after short-term culture of TILs from a metastatic os- teosarcoma. Further studies are needed to déter- mine thé role of gd TcR+ cells in host immune responses directed against this tumor. [less ▲]

Detailed reference viewed: 24 (3 ULg)
Peer Reviewed
See detailPresence Of Growth Hormone-Binding Proteins In Cattle Plasma And Milk
Devolder, A.; Renaville, Robert ULg; Sneyers, M. et al

in Journal of Endocrinology (1993), 138(1),

Detailed reference viewed: 10 (1 ULg)
Full Text
Peer Reviewed
See detailPresence of histone H1 and the electric birefringence behavior of nucleosome fractions from sucrose gradient and column chromatography
Houssier, Claude ULg; Hacha, renée; Gillet, Marie-Claire ULg et al

in International Journal of Biological Macromolecules (1981), 3(1), 3159-62

Differences in H1 content and electric birefringence properties are observed in chromatin fractions and can explain tail folding against the nucleosome core.

Detailed reference viewed: 4 (1 ULg)
Full Text
Peer Reviewed
See detailPresence of human antibodies reacting with Candida albicans O-linked oligomannosides revealed by using an enzyme-linked immunosorbent assay and neoglycolipids
Hayette, Marie-Pierre ULg; Strecker, Gerald; Faille, Christine et al

in Journal of Clinical Microbiology (1992), 30(2), 411-417

In order to study the presence of antibodies directed against Candida albicans O-linked oligomannosides (oligomannosides O) in patient sera, we have developed an enzyme-linked immunosorbent assay (ELISA ... [more ▼]

In order to study the presence of antibodies directed against Candida albicans O-linked oligomannosides (oligomannosides O) in patient sera, we have developed an enzyme-linked immunosorbent assay (ELISA) involving neoglycolipids constructed with these residues (NGLO). Oligomannosides O released by mild alkaline degradation of the C. albicans cell wall phosphopeptidomannan (PPM) contained one to seven mannose residues, among which the quantitatively major components, mannobiose and mannotriose, were shown by 1H nuclear magnetic resonance to contain exclusively alpha (1-2) linkages. The pool of oligomannosides was converted to neoglycolipids by coupling them to 4-hexadecylaniline in an equimolar reaction checked by thin-layer chromatography. We have tested against these neoantigens, coated on ELISA plates, 15 pairs of sera corresponding to individual seroconversions observed in 15 patients during the course of a mycological and serological survey of candidiasis. For all patients, seroconversions resulted in an increased level of antibodies against NGLO. A significant correlation was observed between the results of ELISA-NGLO, ELISA involving the original PPM molecule, and routine antibody detection tests, indirect immunofluorescence assay, and cocounterimmunoelectrophoresis. These results therefore demonstrate the synthesis of human antibodies reactive with oligomannosides O constitutive of the C. albicans mannan molecule which have been previously described as exhibiting an inhibitory effect on human lymphocytic proliferation. [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detailPresence of IL-5 and Ige-antibodies to staphylococcal enterotoxins in nasal polyps is associated with co-morbid asthma
Bachert, Claus; Zhang, Nan; Holtappels, Gabriele et al

in Journal of Allergy and Clinical Immunology (The) (2010), 126(5), 962-968

Detailed reference viewed: 21 (7 ULg)
Full Text
Peer Reviewed
See detailPresence of Oestrogen Receptor Type Beta in Human Retina
Munaut, Carine ULg; Lambert, Vincent ULg; Noël, Agnès ULg et al

in British Journal of Ophthalmology (2001), 85(7), 877-82

BACKGROUND/AIMS: Recent studies have demonstrated the existence of two oestrogen receptor subtypes alpha (ORalpha) and beta (ORbeta) with significant differences of expression among organs. Since ... [more ▼]

BACKGROUND/AIMS: Recent studies have demonstrated the existence of two oestrogen receptor subtypes alpha (ORalpha) and beta (ORbeta) with significant differences of expression among organs. Since important pathologies of human eye could be linked to hormonal status, the expression of ORbeta in ocular posterior segment was sought. METHODS: Immunohistochemical localisation of ORbeta and ORalpha protein and detection of OR mRNAs by reverse transcription-polymerase chain reaction (RT-PCR) were performed in macular and extramacular regions of the retina and in the choroid on male and female donors eyes. RESULTS: ORbeta protein was localised in the ganglion cell layer and in the choroid. At the transcriptional level, mRNA for ORbeta and for ORalpha were both present. Local differences in the expression level were observed, however, suggesting the possibility of variation in the ratio of ORalpha v ORbeta. CONCLUSIONS: The coexistence of two oestrogen receptor subtypes in the human ocular posterior segment raises acute questions about their potential physiological role, but offers a perspective for preferential targeting of a specific receptor subtype. [less ▲]

Detailed reference viewed: 99 (39 ULg)
Peer Reviewed
See detailPresence of pap-, sfa- and afa-related sequences in necrotoxigenic Escherichia coli isolates from cattle : evidence for new variants of the AFA family
Mainil, Jacques ULg; Jacquemin, E.; Hérault, F. et al

in Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Vétérinaire (1997), 61

Detailed reference viewed: 4 (0 ULg)
See detailPresence of RNA and rRNA in the interchromatin granule clusters
Thiry, Marc ULg

Conference (1993)

Detailed reference viewed: 1 (0 ULg)
Full Text
Peer Reviewed
See detailPresence of simian virus 40 DNA sequences in diffuse large B-cell lymphomas in Tunisia correlates with aberrant promoter hypermethylation of multiple tumor suppressor genes
Amara, Khaled; Trimeche, Mounir; Ziadi, Sonia et al

in International Journal of Cancer = Journal International du Cancer (2007), 121(12), 2693-2702

Detailed reference viewed: 16 (0 ULg)
Peer Reviewed
See detailPresence of six bee viruses in Apis mellifera L. populations in Wallonia (Belgium)
Mignon, Jacques ULg; Snoeck, Chantal; Nguyen, Bach Kim ULg et al

Poster (2008)

Detailed reference viewed: 14 (5 ULg)
Full Text
Peer Reviewed
See detailPresenec of extended-spectrum beta-lactamase-producing Enterobacteriaceae in the fecal flora of patients from general practice
MEEX, Cécile ULg; MELIN, Pierrette ULg; Docquier, J. D. et al

in ECCMID (Ed.) Program and Abstracts book of the 18th ECCMID (2008, April)

Detailed reference viewed: 29 (7 ULg)