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See detailThe prolactin gene is located on chromosome 6 in humans
Owerbach, D.; Rutter, W. J.; Cooke, N. E. et al

in Science (1981), 212(4496), 815-6

The gene for prolactin has been located on chromosome 6 in humans. DNA fragments of 4.8 and 4.0 kilobases containing prolactin gene sequences were identified in human genomic DNA, whereas DNA fragments of ... [more ▼]

The gene for prolactin has been located on chromosome 6 in humans. DNA fragments of 4.8 and 4.0 kilobases containing prolactin gene sequences were identified in human genomic DNA, whereas DNA fragments of 7.4, 3.6, and 3.3 kilobases containing prolactin gene sequences were found in mouse cells. In somatic cell hybrids of human and mouse cells the 7.4-, 3.6-, and 3.3-kilobase mouse fragments were always present, whereas the 4.8- and 4.0-kilobase human fragments were only present when human chromosome 6 was also present. We conclude that the prolactin gene resides on chromosome 6, a different location from those of the genes for the related hormones chorionic somatomammotropin and growth hormone. [less ▲]

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See detailProlactin-deficient variants of GH3 rat pituitary tumor cells: linked expression of prolactin and another hormonally responsive protein in GH3 cells
Ivarie, R. D.; Morris, J. A.; Martial, Joseph ULg

in Molecular & Cellular Biology (1982), 2(2), 179-89

GH3 cells normally synthesize and secrete two pituitary polypeptide hormones, prolactin and growth hormone. From an ethyl methane sulfonate-mutagenized population, prolactin low-producing variants have ... [more ▼]

GH3 cells normally synthesize and secrete two pituitary polypeptide hormones, prolactin and growth hormone. From an ethyl methane sulfonate-mutagenized population, prolactin low-producing variants have been isolated at a frequency near 20%. Intracellular prolactin synthesis in the variants was reduced 40- to 100-fold compared to wild-type cells while growth hormone synthesis varied less than 2-fold. This decrease was paralleled by a decrease in intracellular preprolactin mRNA. Although reduced, prolactin synthesis was still repressible by glucocorticoids. There was a coordinate loss of expression of p21, a thyroid and glucocorticoid hormone-regulated protein, in GH3 cells, whereas the synthesis and regulation of other hormonally responsive proteins were unimpaired in the variants. Since p21 expression was coordinately regained in a high-producing prolactin revertant cell, expression of the two proteins is tightly coupled in GH3 cells. The stability of the low-producing phenotype differed among variants. One (B2) gave rise to revertants at about 20% frequency even after two rounds of subcloning, whereas another (B3) was more stable in that only 1 weak revertant was found in 47 subclones. The reversion frequency of B3 cells was also measured at less than 0.5%. Unmutagenized GH3 cells were phenotypically stable in that no prolactin-deficient variant was found among 57 subclones. Since variants were ony found after ethyl methane sulfonate mutagenesis, the DNA alkylating agent appears to have promoted an epigenetic change in pituitary gene expression. [less ▲]

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See detailProlactin-induced activation of nuclear factor kappa B in bovine mammary epithelial cells: Role in chronic mastitis
Boutet, Philippe ULg; Sulon, Joseph ULg; Closset, R. et al

in Journal of Dairy Science (2007), 90(1), 155-164

We sought to determine whether prolactin (PRL) could influence the neutrophilic inflammation that characterizes chronic mastitis. Most of the genes encoding inflammatory proteins depend on the nuclear ... [more ▼]

We sought to determine whether prolactin (PRL) could influence the neutrophilic inflammation that characterizes chronic mastitis. Most of the genes encoding inflammatory proteins depend on the nuclear factor kappa B (NF-kappa B) for their expression. We addressed the hypothesis that immunomodulatory activities of PRL might arise from an increase in NF-kappa B activity. MAC-T cells, a bovine mammary epithelial cell line, were stimulated with increasing concentrations of bovine PRL ( 1, 5, 25, 125, and 1,000 ng/mL). Level of NF-kappa B binding activity was measured and mRNA was evaluated for IL-1 beta, IL-6, IL-8, granulocyte-macrophage colony-stimulating factor (GMCSF), IFN-gamma, and tumor necrosis factor (TNF)-alpha, cytokines known to require NF-kappa B for their maximal transcription. Prolactin activated NF-kappa B; maximal NF-kappa B activation was weaker with PRL than with TNF-alpha at 30 or 180 min poststimulation. In addition, PRL significantly amplified, in a dose-dependent manner, mRNA expression of IL-1 beta, IL-6, IL-8, GMCSF, and TNF-a. We measured PRL concentrations in blood and milk from healthy and chronic mastitis-infected cows, and studied the relationship between the PRL concentration and the degree of inflammation in the mammary gland as indirectly assessed by somatic cell counts (SCC). Plasma PRL did not differ significantly between healthy and chronic mastitis-affected cows (63.7 and 67.5 ng/mL, respectively). Milk PRL concentration was significantly increased in chronic mastitis-affected quarters with the highest SCC, and had a positive significant correlation between SCC, as well as between the number of neutrophils present in milk samples. The present findings show that PRL promotes an inflammatory response in bovine mammary epithelial cells via NF-kappa B activation, and suggest a role for PRL in the pathogenesis of chronic mastitis. [less ▲]

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See detailProlactin/growth hormone-derived antiangiogenic peptides highlight a potential role of tilted peptides in angiogenesis
Nguyen, Ngoc-Quynh-Nhu ULg; Tabruyn, Sébastien ULg; Lins, Laurence ULg et al

in Proceedings of the National Academy of Sciences of the United States of America (2006), 103(39), 14319-14324

Angiogenesis is a crucial step in many pathologies, including tumor growth and metastasis. Here, we show that tilted peptides exert antiangiogenic activity. Tilted (or oblique-oriented) peptides are short ... [more ▼]

Angiogenesis is a crucial step in many pathologies, including tumor growth and metastasis. Here, we show that tilted peptides exert antiangiogenic activity. Tilted (or oblique-oriented) peptides are short peptides known to destabilize membranes and lipid cores and characterized by an asymmetric distribution of hydrophobic residues along the axis when helical. We have previously shown that 16-kDa fragments of the human prolactin/growth hormone (PRL/GH) family members are potent angiogenesis inhibitors. Here, we demonstrate that all these fragments possess a 14-aa sequence having the characteristics of a tilted peptide. The tilted peptides of human prolactin and human growth hormone induce endothelial cell apoptosis, inhibit endothelial cell proliferation, and inhibit capillary formation both in vitro and in vivo. These antiangiogenic effects are abolished when the peptides' hydrophobicity gradient is altered by mutation. We further demonstrate that the well known tilted peptides of simian immunodeficiency virus gp32 and Alzheimer's beta-amyloid peptide are also angiogenesis inhibitors. Taken together, these results point to a potential new role for tilted peptides in regulating angiogenesis. [less ▲]

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See detailProlactine triggers a pro-inflamatory response in bovine mammary epithelial cells
Boutet, Philippe ULg; Sulon, Joseph; Detilleux, Johann ULg et al

in Proceedings: 24th World Buiatrics Congress (2006)

Detailed reference viewed: 32 (3 ULg)
See detailLa Prolactine
Beckers, Albert ULg

Scientific conference (1994, September 22)

Detailed reference viewed: 2 (0 ULg)
See detailProlactinomas
Ciccarelli, A.; Beckers, Albert ULg

in 14th EFES Postgraduate Course in Clinical Endocrinology - Abstract book (2005)

See detailProlactinomas : Advances in Treatment of Endocrine Diseases
Beckers, Albert ULg

Scientific conference (2005, November)

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See detailProlactinomas Resistant to Standard Doses of Cabergoline : A multicenter study of 92 patients
VROONEN, Laurent ULg; Jaffrain-Rea, ML; PETROSSIANS, Patrick ULg et al

in European Journal of Endocrinology (2012), 167

Detailed reference viewed: 41 (9 ULg)
See detailLes prolactinomes résistants aux agents dopaminergiques
Beckers, Albert ULg; Tamagno, J. L.

Scientific conference (2006, December 15)

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See detailProlactinomes résistants aux dopaminergiques
Beckers, Albert ULg

Scientific conference (2011, May 27)

Detailed reference viewed: 8 (2 ULg)
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See detailProlégomènes à l'étude de la Schola du Trajan à Ostie.
Morard, Thomas ULg; Wavelet, David

in Mélanges de l'Ecole Française de Rome. Italie et Méditérranée (2002), 114(2), 759-815

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See detail"Prolégomènes à l'études du monologue"
Defays, Jean-Marc ULg

in Humoresques (Association pour le développement des recherches sur le Comique, le Rire et l'Humour) (2011), (33), 23-37

Detailed reference viewed: 26 (1 ULg)
See detailProlégomènes à une étude de la modalité en néo-égyptien
Polis, Stéphane ULg

Master's dissertation (2004)

Detailed reference viewed: 22 (6 ULg)
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See detailProlégomènes à une philosophie de la médiation
Bachelet, Jean-François ULg

in Castelain, Bernard (Ed.) De l'autre côté du conflit: la médiation (2013)

Detailed reference viewed: 26 (4 ULg)
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See detailProlégomènes à une théorie unifiée de la temporalité en égyptien
Winand, Jean ULg

in Proceedings of the Seventh International Congress of Egyptologists (1998)

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See detailProlepsis : Foreword
Longrée, Dominique ULg; Halla-Aho, Hilla

in De Lingua Latina : Revue de Linguistique Latine du Centre Alfred Ernout (2012), 7

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See detailProliferation generation of auditory hair cells in culture
Malgrange, B; Belachew, S; Thiry, Marc ULg et al

in Acta Oto-Rhino-Laryngologica Belgica (2002), 56

Detailed reference viewed: 7 (0 ULg)