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See detailA Molecular Biologic Study of Extracellular Matrix Components During the Development of Glomerulosclerosis in Murine Chronic Graft-Versus-Host Disease
Munaut, Carine ULg; Bergijk, E. C.; Baelde, J. J. et al

in Laboratory Investigation : Journal of Technical Methods & Pathology (1992), 67(5), 580-7

BACKGROUND: We studied the development of glomerulosclerosis in murine chronic graft-versus-host disease, a model for human systemic lupus erythematosus. EXPERIMENTAL DESIGN: The disease was induced in ... [more ▼]

BACKGROUND: We studied the development of glomerulosclerosis in murine chronic graft-versus-host disease, a model for human systemic lupus erythematosus. EXPERIMENTAL DESIGN: The disease was induced in (C57BL10 x DBA/2)F1 hybrids by injection of DBA/2 lymphocytes leading to deposition of auto-antibodies in the glomeruli, and a lupus type of nephritis morphologically. We have determined the levels of mRNA coding for laminin (B1 and B2), a 67 kilodalton laminin binding protein, and types I and IV collagen, in control and graft-versus host disease mice at various times after disease induction. RESULTS: Laminin and collagen mRNAs were increased in whole kidneys 4 weeks after induction of the disease. At week 10, all animals displayed dramatic stimulation of alpha 1(I), alpha 1(IV), laminin B1, and B2 mRNAs. The 67 kilodalton laminin binding protein mRNA was also doubled from week 4 to 16. In isolated glomeruli, the mRNA level coding for laminin B2 was already significantly increased from week 8. This enhancement of laminin synthesis corresponds to the mesangial expansion and to the development of laminin-containing spike formations of the glomerular basement membrane at week 8. CONCLUSIONS: The expansion of the mesangial matrix in murine chronic graft-versus-host disease is caused at least in part, by an increased production of extracellular matrix components by glomerular cells. These results demonstrate that the increase of specific extracellular matrix components mRNAs precedes light microscopic changes. Quantitative evaluation of the mRNA levels coding for extracellular matrix proteins may reveal a useful method for the early detection of the development of glomerular sclerosis at the stage preceding the onset of anatomo-clinical changes. [less ▲]

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See detailMolecular biology contribution to animal production in 1994
Renaville, Robert ULg

Conference (1994)

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See detailMolecular biology in support of an old paradigm about the induction of flowering
Périlleux, Claire ULg

in Flowering Newsletter (1995), 20

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See detailMolecular Biology of Antimicrobial resistance
Melin, Pierrette ULg

Conference (1998, October)

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See detailMolecular Biology of Bovine Herpesvirus Type 4
Thiry, Etienne ULg; Bublot, M.; Dubuisson, J. et al

in Veterinary Microbiology (1992), 33(1-4), 79-92

Bovine herpesvirus type 4 (BHV-4) is a ubiquitous virus of cattle. Its genome is a 144 +/- 6 kb double-stranded DNA consisting of a unique central part (L-DNA) flanked at both ends by tandem repeats ... [more ▼]

Bovine herpesvirus type 4 (BHV-4) is a ubiquitous virus of cattle. Its genome is a 144 +/- 6 kb double-stranded DNA consisting of a unique central part (L-DNA) flanked at both ends by tandem repeats called polyrepetitive DNA (prDNA or H-DNA). The overall arrangement of genes has been obtained by the analysis of homologies between short BHV-4 DNA sequences and corresponding genes of Epstein-Barr virus (EBV) and herpesvirus saimiri (HVS). The gene expression is temporally regulated. Glycoprotein precursor p (gp10/gp17) is expressed as gamma 1 polypeptide. Glycoproteins gp1, gp8, gp11 and their precursors are gamma 2 proteins. The analysis of strain variations allows the definition of two types of strains, based on the DNA patterns: the Movar 33/63-like and the DN 599-like strains. Only the M40 strain, isolated in India, fails to fit this classification. The genomic variations have been compiled to build a dendrogram showing three levels of divergence between BHV-4 strains or isolates. The available molecular data indicate that the BHV-4 genome shares much similarity with the DNA of EBV and HVS, two representative members of the gammaherpesvirinae. BHV-4 may therefore be classified in the subfamily gammaherpesvirinae. [less ▲]

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See detailMolecular biology of HCV: past, present and future perspective
BONTEMS, Sébastien ULg

Conference (2014, October 16)

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See detailMolecular Biology of tilapia prolactins
Swennen, E.; Sekkali, B.; Poncelet, A. C. et al

in Fundamental and applied research for aquaculture (1993)

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See detailMolecular biology, phytochemistry and bioactivity of three endemic Aloe species from Mauritius and Reunion Islands.
Ranghoo-Sanmukhiya, M.; Govinden-Soulange, J.; Lavergne, C. et al

in Phytochemical Analysis (2010)

Introduction - Aloe tormentorii, A. purpurea and A. macra are used as multipurpose folk medicines in Reunion and Mauritius Islands and are mistaken for the introduced Aloe vera.Objective - To compare the ... [more ▼]

Introduction - Aloe tormentorii, A. purpurea and A. macra are used as multipurpose folk medicines in Reunion and Mauritius Islands and are mistaken for the introduced Aloe vera.Objective - To compare the phytochemical, antimicrobial and DNA profiles of Aloe endemic to Mauritius and Reunion with the profiles of A. vera.Methodology - Leaf extracts of these Aloe species were analysed using standard phytochemical screening techniques, TLC and by HPLC. These extracts were also assayed for antimicrobial activity using microdilution techniques. Genetic diversity was studied using RAPD markers.Results - Phytochemical and antimicrobial assays and RAPD analysis showed that Mascarene Aloe species were very different from A. vera.Conclusion - This study is the first report highlighting the differences between Aloe sp.p from Mascarene and Aloe vera at the metabolic and genomic level. Copyright (c) 2010 John Wiley & Sons, Ltd. [less ▲]

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See detailMolecular biomimetics applied to medical devices
Van de Weerdt, Cécile ULg; Vreuls, Christelle ULg; Genin, Alexis ULg et al

Poster (2012, April 18)

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See detailMolecular biomimetics applied to medical devices
Van de Weerdt, Cécile ULg; Archembeau, Catherine; Vreuls, Christelle ULg et al

Poster (2012, May 05)

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See detailMolecular certification in chicken meat channel.
Haezebroeck, Valérie; Renaville, Robert ULg; Parmentier, Isabelle et al

in Journal of Animal Science (2001), 79(Suppl 1 N°1072), 260

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See detailMolecular characterisation of a versatile peroxidase from a Bjerkandera strain
Moreira, Patricia R.; Duez, Colette ULg; Dehareng, Dominique ULg et al

in Journal of Biotechnology (2005), 118(4), 339-352

The cloning and sequencing of the rbpa gene coding for a versatile peroxidase from a novel Bjerkandera strain is hereby reported. The 1777 bp isolated fragment contained a 1698 bp peroxidase-encoding gene ... [more ▼]

The cloning and sequencing of the rbpa gene coding for a versatile peroxidase from a novel Bjerkandera strain is hereby reported. The 1777 bp isolated fragment contained a 1698 bp peroxidase-encoding gene, interrupted by 11 introns. The 367 amino acid-deduced sequence includes a 27 amino acid-signal peptide. The molecular model, built via homology modelling with crystal structures of four fungal peroxidases, highlighted the amino acid residues putatively involved in manganese binding and aromatic substrate oxidation. The potential heme pocket residues (R44, F47, H48, E79, N85, H177, F194 and D239) include both distal and proximal histidines (H48 and H177). RBP possesses potential calcium-binding residues (D49, G67, D69, S71, S178, D195, T197, I200 and D202) and eight cysteine residues (C3, C15, C16, C35, C121, C250, C286, C316). In addition, RIBP includes residues involved in substrate oxidation: three acidic residues (E37, E41 and D183)-putatively involved in manganese binding and H83 and W172-potentially involved in oxidation of aromatic substrates. Characterisation of nucleotide and amino acid sequences include RBP in versatile peroxidase group sharing catalytic properties of both UP and MnP. In addition, the RBP enzyme appears to be closely related with the ligninolytic peroxidases from the Trametes versicolor strain. (C) 2005 Published by Elsevier B.V. [less ▲]

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See detailMolecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues
Lakaye, Bernard ULg; Makarchikov, Alexander F; Antunes, Adelio F et al

in Journal of Biological Chemistry (2002), 277(16), 13771-13777

Thiamine triphosphate (ThTP) is found at low concentrations in most animal tissues, and recent data suggest that it may act as a phosphate donor for the phosphorylation of some proteins. In the mammalian ... [more ▼]

Thiamine triphosphate (ThTP) is found at low concentrations in most animal tissues, and recent data suggest that it may act as a phosphate donor for the phosphorylation of some proteins. In the mammalian brain, ThTP synthesis is rapid, but its steady-state concentration remains low, presumably because of rapid hydrolysis. In this report we purified a soluble thiamine triphosphatase (ThTPase; EC 3.6.1.28) from calf brain. The bovine ThTPase is a 24-kDa monomer, hydrolyzing ThTP with virtually absolute specificity. Partial sequence data obtained from the purified bovine enzyme by tandem mass spectrometry were used to search the GenBank(TM) data base. A significant identity was found with only one human sequence, the hypothetical 230-amino acid protein MGC2652. The coding regions from human and bovine brain mRNA were amplified by reverse transcription-PCR, cloned in Escherichia coli, and sequenced. The human open reading frame was expressed in E. coli as a GST fusion protein. Transformed bacteria had a high isopropyl-beta-D-thiogalactopyranoside-inducible ThTPase activity. The recombinant ThTPase had properties similar to those of human brain ThTPase, and it was specific for ThTP. The mRNA was expressed in most human tissues but at relatively low levels. This is the first report of a molecular characterization of a specific ThTPase. [less ▲]

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See detailMOLECULAR CHARACTERIZATION OF CLOSTRIDIUM DIFFICILE STRAINS FROM ELDERLY CARE HOME RESIDENTS
Rodriguez Diaz, Cristina ULg

Poster (2013, October 11)

Clostridium difficile is the primary cause of nosocomial diarrhoea and pseudomembranous colitis after use of antibiotics. Production of toxins A and B are the main virulence factors responsible for its ... [more ▼]

Clostridium difficile is the primary cause of nosocomial diarrhoea and pseudomembranous colitis after use of antibiotics. Production of toxins A and B are the main virulence factors responsible for its pathogenicity. Increasing age, several co-morbidities, environmental contamination, antibiotic exposure and other intestinal perturbations appear to be the greatest risk factors for C. difficile infection (CDI). Therefore, elderly care home residents are considered particularly vulnerable to CDI. The aim of this study was to evaluate and follow the prevalence of C. difficile in a Belgian nursing home and to characterize the C. difficile strains isolated from faeces of elderly care home residents. [less ▲]

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See detailMolecular characterization of double minutes/homogeneously staining regions in selected tumor cell lines
Conteduca, Giuseppina ULg; Trombetta, Domenico; Storlazzi, Tiziana C et al

in Trombetta, Domenico; Lonoce, Angelo; Guastadisegni, Maria C (Eds.) et al the official journal of the International Society for Cellular Oncology (2008, January 04)

Double minutes (dmin) and homogeneously staining regions (hsr) are the cytogenetic hallmarks of genomic amplification in cancer. Different mechanisms have been proposed to explain their genesis. Recently ... [more ▼]

Double minutes (dmin) and homogeneously staining regions (hsr) are the cytogenetic hallmarks of genomic amplification in cancer. Different mechanisms have been proposed to explain their genesis. Recently, our group showed that the MYC-containing dmin in leukemia cases arise by excision and amplification (episome model). In the present paper we investigated 10 cell lines from solid tumors showing MYCN amplification as dmin or hsr. Particularly revealing results were provided by the two subclones of the neuroblastoma cell line STA-NB-10, one showing dmin-only and the second hsr-only amplification. Both subclones showed a deletion, at 2p24.3, whose extension matched the amplicon extension. Additionally, the amplicon structure of the dmin and hsr forms was identical. This strongly argues that the episome model, already demonstrated in leukemias, applies to solid tumors as well, and that dmin and hsr are two faces of the same coin. The organization of the duplicated segments varied from very simple (no apparent changes from the normal sequence) to very complex. MYCN was always overexpressed (significantly overexpressed in three cases). The fusion junctions, always mediated by nonhomologous end joining, occasionally juxtaposed truncated genes in the same transcriptional orientation. Fusion transcripts involving NBAS (also known as NAG), FAM49A, BC035112 (also known as NCRNA00276), and SMC6 genes were indeed detected, although their role in the context of the tumor is not clear. [less ▲]

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See detailMolecular characterization of embryogenesis in Phaseolus
Abid, Ghassen ULg

Doctoral thesis (2011)

Chez les végétaux supérieurs, l’embryogenèse est une phase clé du développement au cours de laquelle l’embryon établit les principales structures de la future plante. La compréhension des processus ... [more ▼]

Chez les végétaux supérieurs, l’embryogenèse est une phase clé du développement au cours de laquelle l’embryon établit les principales structures de la future plante. La compréhension des processus moléculaires et physiologiques menant à la formation de la graine est donc d’un intérêt agronomique majeur. Chez Phaseolus la caractérisation moléculaire de l’embryogenèse permet de mieux comprendre les mécanismes du développement embryonnaire et de son dysfonctionnement observé chez les hybrides interspécifiques. Cette thèse s’inscrit dans ce cadre et vise à identifier et caractériser des gènes clés impliqués dans le développement de l'embryon chez Phaseolus. Des hybridations interspécifiques ont été réalisées entre l’espèce P.vulgaris L. (cultivar NI637) utilisée comme parent mâle et l’espèce P. coccineus L. (cultivar NI16) utilisée comme parent femelle. Des analyses ont aussi été effectuées sur un mutant obtenu par mutagenèse chimique à l'EMS (Ethyl Méthyl Sulfonate) de graines de la variété BAT93 de P.vulgaris. Une étude histologique comparative a permis de suivre la dynamique de l’embryogenèse du haricot commun à partir d’embryons prélevés 3 à 12 jours après la pollinisation et provenant de plantes normales et déficients dans la production de graines. Les embryons de P. vulgaris se développent plus rapidement par rapport à ceux issus du mutant EMS. Ces derniers présentent des anomalies au niveau de l’embryon et du suspenseur. La caractérisation fonctionnelle de deux gènes candidats MIPS (myo-inositol phosphate synthase) et Sus (sucrose synthase) a été réalisée par RT-PCR quantitative et hybridation in situ suite à une étude spatio-temporelle d’expression de ces deux gènes candidats au cours de développement embryonnaire chez Phaseolus. L’analyse du profil d’expression de ces deux gènes montre qu’ils sont exprimés différemment au niveau des tissus de l’embryon et du suspenseur. L’analyse in silico nous a permis de sélectionner 22 gènes candidats dont nous avons vérifié l'expression au cours de développement de la graine chez Phaseolus. Des variations au niveau de la méthylation de l’ADN ont été déterminées chez les hybrides interspécifiques comparativement à leurs parents. La technique de l’HSS a permis d’isoler des fragments d’ADNs complémentaires différemment exprimés au cours de développement de la graine chez Phaseolus. L’analyse des séquences de ces ADNs complémentaires montre qu’ils codent pour plusieurs protéines intervenant dans le développement cellulaire et embryonnaire, en particulier le "storage protein activator" (SPA), le "pentatricopeptide repeat-containing protein" (PPR) et l’acetyl-CoA carboxylase (ACCase). La caractérisation de ces différents gènes exprimés au cours du développement de la graine, fournit de nouveaux outils susceptibles de mettre en évidence des mécanismes de dysfonctionnement embryonnaire chez le genre Phaseolus. [less ▲]

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See detailMolecular characterization of geminivirus-derived small RNAs in different plant species.
Akbergenov, Rashid; Si-Ammour, Azeddine; Blevins, Todd et al

in Nucleic acids research (2006), 34(2), 462-71

DNA geminiviruses are thought to be targets of RNA silencing. Here, we characterize small interfering (si) RNAs-the hallmarks of silencing-associated with Cabbage leaf curl begomovirus in Arabidopsis and ... [more ▼]

DNA geminiviruses are thought to be targets of RNA silencing. Here, we characterize small interfering (si) RNAs-the hallmarks of silencing-associated with Cabbage leaf curl begomovirus in Arabidopsis and African cassava mosaic begomovirus in Nicotiana benthamiana and cassava. We detected 21, 22 and 24 nt siRNAs of both polarities, derived from both the coding and the intergenic regions of these geminiviruses. Genetic evidence showed that all the 24 nt and a substantial fraction of the 22 nt viral siRNAs are generated by the dicer-like proteins DCL3 and DCL2, respectively. The viral siRNAs were 5' end phosphorylated, as shown by phosphatase treatments, and methylated at the 3'-nucleotide, as shown by HEN1 miRNA methylase-dependent resistance to beta-elimination. Similar modifications were found in all types of endogenous and transgene-derived siRNAs tested, but not in a major fraction of siRNAs from a cytoplasmic RNA tobamovirus. We conclude that several distinct silencing pathways are involved in DNA virus-plant interactions. [less ▲]

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See detailMolecular characterization of the AMPA-receptor potentiator S70340 in rat primary cortical culture: Whole-genome expression profiling.
Mourlevat, S.; Galizzi, J. P.; Guigal-Stephan, N. et al

in Neuroscience Research (2011), 70

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See detailMolecular characterization of the bovine GHRL gene
Colinet, Frédéric ULg; Portetelle, Daniel ULg; Renaville, Robert ULg

in Archiv für Tierzucht = Archives Animal Breeding (2009), 52(1), 79-84

Bovine ghrelin, a 27 amino acid pepticle, has been identified in oxyntic glands of the abomasum. It is an endogenous ligand for growth hormone secretagogue receptor and stimulates food intake and growth ... [more ▼]

Bovine ghrelin, a 27 amino acid pepticle, has been identified in oxyntic glands of the abomasum. It is an endogenous ligand for growth hormone secretagogue receptor and stimulates food intake and growth hormone secretion. The bovine GHRL gene was completely sequenced and consists of five exons and four introns. Like mouse and human GHRL genes, we found that the bovine GHRL gene also contains a first non-coding exon of 21 bp. The bovine GHRL gene codes for 116 amino acid pepticle named preproghrelin which contains the ghrelin pepticle and another pepticle similar to obestatin. Sequence analysis revealed eight polymorphisms, which are located in the non-coding sequence of the gene. [less ▲]

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