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See detailProduction and freeze-drying of leben lactic starter
Ziadi, M.; Mhir, S.; Abdelsam, M. et al

in Research in Microbiology (2009), 4(1), 31-37

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See detailProduction and kinematics of sodium atoms in the coma of comet Hale-Bopp
Arpigny, Claude ULg; Rauer, H.; Manfroid, Jean ULg et al

in Astronomy and Astrophysics (1998), 334(Letters), 53-56

High-resolution spectra of sodium D line emission in comet Hale-Bopp (C/1995 O1) were obtained at the Observatoire de Haute-Provence, France, on 25-27 March and on 15-17 April, 1997. The observations have ... [more ▼]

High-resolution spectra of sodium D line emission in comet Hale-Bopp (C/1995 O1) were obtained at the Observatoire de Haute-Provence, France, on 25-27 March and on 15-17 April, 1997. The observations have been used to measure the velocity of sodium atoms in the coma within 2 10(5) km from the nucleus. A comparison between the March and April data provides an illustration of the influence of the heliocentric radial velocity on the strength of the fluorescence (Swings effect), and on the velocity of Na atoms achieved by solar radiation pressure acceleration. Evidence for the presence of a distributed source in the coma is found from the relatively high tailward velocities on the sunward side of the coma, in addition to the sunward extent of sodium emission up to 1.4 10(5) km in April. Based on observations secured at OHP (France) [less ▲]

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See detailThe production and localization of laminin in cultured vascular and corneal endothelial cells.
Gospodarowicz, D.; Greenburg, G.; Foidart, Jean-Michel ULg et al

in Journal of Cellular Physiology (1981), 107(2), 171-83

The production and localization of laminin, as a function of cell density (sparse versus confluent cultures) and growth stage (actively growing versus resting cultures), has been compared on the cell ... [more ▼]

The production and localization of laminin, as a function of cell density (sparse versus confluent cultures) and growth stage (actively growing versus resting cultures), has been compared on the cell surfaces of cultured vascular and corneal endothelial cells. Comparison of the abilities of the two types of cells to secrete laminin and fibronectin into their incubation medium reveals that vascular endothelial cells can secrete 20-fold as much laminin as can corneal endothelial cells. In contrast, both cell types produce comparable amounts of fibronectin. Furthermore, if one compares the secretion of laminin and fibronectin as a function of cell growth, it appears that the laminin released into the medium by either vascular or corneal endothelial cells, is a function of cell density and cell growth, since this release is most pronounced when the cells are sparse and actively growing, and decreases by 10- and 30-fold, respectively, when either vascular or corneal endothelial cell cultures become confluent. With regard to fibronectin secretion, no such variation can be seen with vascular endothelial cell cultures, regardless of whether they are sparse and actively growing or confluent and resting. Corneal endothelial cell cultures, demonstrated a twofold increase in fibronectin production when they were confluent and resting as compared to when they were sparse and actively growing. When the distribution of laminin versus fibronectin within the apical and basal cell surfaces of cultured corneal and vascular endothelial cells is compared, one can observe that unlike fibronectin, which in sparse and subconfluent cultures can be seen to be associated with both the apical and basal cell surfaces, laminin does not ever seem to be present on the apical cell surface. In confluent cultures, laminin can be found associated primarily with the extracellular matrix beneath the cell monolayer, where it codistributes with type IV collagen. [less ▲]

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See detailProduction and marketing constraints of dairy farmers in Son La milk value chain, Vietnam
Nguyen Hung, Anh; Tran Huu, Cuong; Bui, Thi Nga ULg

in Greener Journal of Business and Management Business Studies (2013), 3(1), 31-37

Rural livelihood augmentation has long been a crucial challenge for the effectiveness of Vietnamese government policies in agricultural sector. Despite the amazing fact that farmers’ participation in the ... [more ▼]

Rural livelihood augmentation has long been a crucial challenge for the effectiveness of Vietnamese government policies in agricultural sector. Despite the amazing fact that farmers’ participation in the dairy value chain has promised the better outcome, there are still millions of rural farmer households struggling against the inefficient production and marketing, and decline with hope of improving their main source of income. Plus the extreme price volatility and the market power of downstream actors, rural dairy farmers with small-scale production have verged to the edge of selling up the whole supply chain. This paper attempted to analyze the production and marketing constraints faced by the local dairy farmers in the milk value chain of Northern mountainous Sonla provinces of Vietnam. The Pearson’s chi-square test was employed to assess the statistical significance of farmer’s responses across the study sites. The results of the study revealed farmers produce and different marketing different qualities of milk to two main buyers under the constraints of high input prices, feed scarcity, exploitation by downstream actors, capital investment inadequacy, inadequate poor market information and knowledge, lack of technical support from dairy manufacturer and local authority, land limit, and cow diseases. [less ▲]

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See detailProduction and oil-emulsion formulation of Cadophora malorum and Alternaria jacinthicola, two biocontrol agents against Water Hyacinth (Eichhornia crassipes)
Dagno, Karim ULg; Lahlali, Rachid; Diourté, Mamourou et al

in African Journal of Microbiology Research [=AJMR] (2011), 5(8), 924-929

Cadophora malorum isolate Mln715 and Alternaria jacinthicola strain MUCL 53159 are under <br />development as biocontrol agents against Water Hyacinth (Eichhornia crassipes) in Mali. Production of spores ... [more ▼]

Cadophora malorum isolate Mln715 and Alternaria jacinthicola strain MUCL 53159 are under <br />development as biocontrol agents against Water Hyacinth (Eichhornia crassipes) in Mali. Production of spores of these agents on locally available substrates (Water Hyacinth, powdered paddy rice chaff, wheat semolina) was assessed with a view to mass production. The C. malorum isolate sporulated best on Water Hyacinth (4.08 × 107 spores ml-1), followed by wheat (1.06 ×107 spores ml-1), whereas A. jacinthicola produced more spores on paddy rice chaff and wheat (0.24 x 107 spores ml-1). The severity of the damage caused by each pathogen was evaluated in the greenhouse and in the field. Under both greenhouse and field conditions, the biocontrol efficacy of the fungal isolates was improved with (unrefined) Carapa procera (L.) oil or (refined) palm oil, supplemented with soybean lecithin and Tween 20. When such a formulation was used, the incubation time was 4 to 5 days in the greenhouse and 7 to 9 days on the field, and the damage severity (DS) recorded 6 weeks after treatment varied from 87.02 to 93.13% in the greenhouse and from 59.11 to 63.00% in the field. For unformulated C. malorum and A. jacinthicola respectively, the incubation times were longer and the DS values were only 22.11 and 29.05% in the greenhouse and 12.05 and 15.15% on the field. Our results highlight good substrates for mass production of these mycoherbicides and demonstrate the ability of vegetable oil formulations to improve their efficacy. [less ▲]

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See detailProduction and partial characterization of chitinase from a halotolerant Planococcus rifitoensis strain M2-26
Essghaier, Badiaa; Rouaissi, Mustapha; Boudabous, Abdellatif et al

in World Journal of Microbiology & Biotechnology (2010), 26(6), 977-984

This paper is the first to investigate the production and partial characterization of the chitinase enzyme from a moderately halophilic bacterium Planococcus rifitoensis strain M2-26, earlier isolated ... [more ▼]

This paper is the first to investigate the production and partial characterization of the chitinase enzyme from a moderately halophilic bacterium Planococcus rifitoensis strain M2-26, earlier isolated from a shallow salt lake in Tunisia. The impact of salt, salinity concentration, pH, carbon and nitrogen sources on chitinase production and activity have been determined. This is the first report on a high salt-tolerant chitinase from P. rifitoensis, since it was active at high salinity (from 5 to 30% NaCl) as well as in the absence of salt. This enzyme showed optimal activity at 70 C and retained up to 82 and 66% of its original activity at 80 or 90 C, respectively. The activity of the enzyme was also shown over a wide pH range (from 5 to 11). For characterization of the enzyme activity, the chitinase secreted in the culture supernatant was partially purified. The preliminary study of the concentrated dialysed supernatant on native PAGE showed at least three chitinases produced by strain M2-26, with highest activity approximately at 65 kDa. Thus, the thermo-tolerant and high salt-tolerant chitinases produced by P. rifitoensis strain M2-26 could be useful for application in diverse areas such as biotechnology and agro-industry. [less ▲]

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See detailProduction and processing of canna vermicelli in Hung Yen Province toward sustainable development
Le, Thi Long Vy ULg; Do Truong, Lam; Vu Dinh, Ton et al

in Hanoi University of Agriculture; Francophone Joint University Council (CIUF) (Eds.) Proceedings of Scientific Research Results - Institutional University Cooperation Program 2008-2012 (2013)

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See detailProduction and processing of canna vermicelli in Hung Yen province toward sustainable development
Le Thi Long, Vy; Do Truong, Lam; Vu Dinh, Ton et al

Conference (2011)

Located in the Red River Delta region, Hung Yen province has 923000 ha of total land area, of which agricultural land area occupied 60.67%. The agricultural land area per labor is 0.1 ha. However, it has ... [more ▼]

Located in the Red River Delta region, Hung Yen province has 923000 ha of total land area, of which agricultural land area occupied 60.67%. The agricultural land area per labor is 0.1 ha. However, it has been annually decreasing due to the development of industrial zone, construction of urban zone, expansion of road… For these reasons, the stainable development for production and processing of agricultural products plays a very important role in job creation for farmers who loosed their own agricultural land, avoidance of quantity and quality loosing of raw agricultural products and increase in economic efficiency of agricultural production and processing. The production and processing of canna are traditional jobs of rural households in Hung Yen. They not only have been creating jobs for rural households but also increasing in their income. Beside the social and economic benefit created by these activities, the processing activity has caused many negative consequences. It led to environmental pollution due to its waste, overuse of chemical substances. Desiccated of vermicelli in bad condition resulted in the unsafe food. Therefore, this study is conducted to deeply investigate both positive effects and negative consequences created by production and processing of canna vermicelli in term of economic, social and environmental aspects. This study concentrates on the farm households; discuss advantages and disadvantages of production inputs, processing techniques and output market of their products from arrowroot to vermicelli; and find out solutions for the sustainable development for production and processing of vermicelli. [less ▲]

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See detailProduction and properties of Yarrowia lipolytica lipase.
Destain, Jacqueline ULg; Valepyn, Emmanuel ULg; Roblain, D. et al

Poster (1995, February)

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See detailProduction and purification of biologically active recombinant tilapia (Oreochromis niloticus) prolactins
Swennen, D.; Rentier-Delrue, Françoise ULg; Auperin, B. et al

in Journal of Endocrinology (1991), 131(2), 219-27

Recombinant expression vectors carrying tilapia prolactin-I or -II (tiPRL-I or tiPRL-II) cDNA were constructed and the tiPRL-I and II proteins were produced in E. coli as inclusion bodies. These inclusion ... [more ▼]

Recombinant expression vectors carrying tilapia prolactin-I or -II (tiPRL-I or tiPRL-II) cDNA were constructed and the tiPRL-I and II proteins were produced in E. coli as inclusion bodies. These inclusion bodies were dissolved in 6 mol urea/l. Refolding of the proteins was followed by SDS-PAGE under non-reducing conditions so as to visualize the oxidized state of the molecules. Proteins tiPRL-I and tiPRL-II were purified by gel filtration and ion-exchange chromatography. The N-terminal sequence and bioactivities of both purified proteins were then analysed. Recombinant tiPRL-I and tiPRL-II induced a significant rise in plasma calcium levels as well as in mucocyte density in the abdominal skin epithelium. When tested on kidney membrane, both proteins exhibited potency in competing with 125I-labelled tiPRL-I for binding sites, but tiPRL-I seemed to be more potent than tiPRL-II in competing for these sites. The results obtained for the biological activities tested suggest that both recombinant prolactins were correctly refolded and had retained the full biological activity previously observed with the natural hormone preparations extracted from the animals. [less ▲]

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See detailProduction and purification of siderophores from fluorescent Pseudomonads as chelating substances.
Jacques, Ph.; Ongena, Marc ULg; Delfosse, Ph. et al

Poster (1992, June)

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See detailProduction and toxicity of γ-decalactone and 4-hydroxydecanoic acid from Rhodotorula aurantiaca
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

Poster (2009)

The objective of this study was to compare the production of γ- decalactone in different scales and to study the effects of 4- hydroxydecanoic acid and γ-decalactone on the growth of R. aurantiaca. The ... [more ▼]

The objective of this study was to compare the production of γ- decalactone in different scales and to study the effects of 4- hydroxydecanoic acid and γ-decalactone on the growth of R. aurantiaca. The effect of gum tragacanth on the production of γ-decalactone by R. aurantiaca was evaluated. [less ▲]

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See detailProduction at the Curie Level of No-Carrier-Added 6-18F-Fluoro-L-Dopa
Libert, Lionel ULg; Franci, Xavier; Plenevaux, Alain ULg et al

in Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine (2013), 54(7), 1154-1161

6-18F-fluoro-L-dopa (18F-FDOPA) has proven to be a useful radiopharmaceutical for the evaluation of presynaptic dopaminergic function using PET. In comparison to electrophilic synthesis, the no-carrier ... [more ▼]

6-18F-fluoro-L-dopa (18F-FDOPA) has proven to be a useful radiopharmaceutical for the evaluation of presynaptic dopaminergic function using PET. In comparison to electrophilic synthesis, the no-carrier-added (NCA) nucleophilic method has several advantages. These include much higher available activity and specific activity. Recently, we have described an NCA enantioselective synthesis using a chiral phase-transfer catalyst. However, some chemicals were difficult to implement into a commercially available synthesizer, restricting access to this radiopharmaceutical to only a few PET centers. Methods: In this paper, 2 important chemical improvements are proposed to simplify production of 18F-FDOPA, resulting in straightforward automation of the synthesis in a commercially available module. Results: First, a fast, simple, and reliable synthesis of 2-18F-fluoro-4,5-dimethoxybenzyl iodide on a solid phase support was developed. Second, a phase-transfer catalyst alkylation of a glycine derivative at room temperature was used to enable enantioselective carbon–carbon bond formation. After hydrolysis and high-performance liquid chromatography purification, a high enantiomeric excess of 18F-FDOPA (~97%) was obtained using a chiral catalyst available from a biphenyl 3 substrate. The total synthesis time was 63 min, and the decay-corrected radiochemical yield was 36% +/- 3% (n = 8). Conclusion: By exploiting the advantages of this NCA approach, using a starting activity of 185 GBq of NCA 18F-fluoride, high activities of 18F-FDOPA (> 45 GBq) with high specific activity (>753 GBq/mmol) are now available at the end of synthesis for use in clinical investigations. [less ▲]

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See detailProduction bovine et bien-être animal
Nicks, Baudouin ULg; Dufrasne, M.

Article for general public (1997)

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See detailLa production céréalière biologique en Wallonie
Debode, F.; Schiepers, H.; Burny, Philippe ULg

in Destain, J.-P.; Bodson, B. (Eds.) Livre Blanc "Céréales", édition février 2013 (2013)

Detailed reference viewed: 17 (6 ULg)
See detailProduction d’acide gluconique, reconnu comme molécule prébiotique, par transformation enzymatique à partir d’hydrolysats d’amidon
Schömig, Alexandra; Goffin, Dorothée ULg

Report (2008)

Une méthode tout à fait originale a été mise au point dans le but de produire de l’acide gluconique à partir d’hydrolysats d’amidon (gluco-oligosaccharides en liaisons -(1-4)). En effet, dans de ... [more ▼]

Une méthode tout à fait originale a été mise au point dans le but de produire de l’acide gluconique à partir d’hydrolysats d’amidon (gluco-oligosaccharides en liaisons -(1-4)). En effet, dans de nombreuses industries traitant des produits d’origine végétale, l’amidon constitue un co-produit qu’il est intéressant de valoriser. Dans ce contexte, le présent travail présente une voie de valorisation originale des hydrolysats d’amidon. Cette méthode comporte une première étape qui consiste en l’hydrolyse par voie enzymatique la plus poussée possible des maltooligosaccharides de la fraction amylose dans le but d'obtenir un maximum de glucose. Deux enzymes ont été testées : l’-glucosidase de Bacillus stearothermophilis et l’amyloglucosidase d’Aspergillus niger. Ces dernières ont été testées sur trois différents substrats : le glucose (pour vérifier l’absence d’une activité de transglucosylation dans les conditions de travail), le maltose et un mélange de maltooligosaccharides de DP 1 à 4. Aucune activité de transglucosylation n’est observée. L’-glucosidase hydrolyse le maltose jusqu’à une teneur résiduelle de 44% et les maltooligosaccharides sont clivés suivant des cinétiques proches, de sorte qu’après 5h30 de réaction, il ne reste que 5 à 10 % de ces maltooligosaccharides. L’amyloglucosidase elle hydrolyse très rapidement les DP supérieurs qui forment alors du maltose qui lui limitera fortement la vitesse de réaction. La deuxième étape consiste en la conversion du glucose libéré en acide gluconique à l’aide d’une glucose oxydase. Les conditions optimales de réaction et l’influence de différents paramètres sur la vitesse et le rendement de la réaction ont été étudiés sur des solutions de glucose. Une régulation du pH s’est avérée indispensable pour rester dans les conditions optimales de l’enzyme et l’utilisation de milieux tamponnés à été préféré à celle d’un pHstat. L’influence de l’ajout d’une quantité d’enzyme plus importante sur la vitesse de réaction a été mise en évidence ainsi que l’importance de l’apport en O2 substrat de la réaction. En effet un débit d’air comprimé de 3L/min, permet un taux de conversion du glucose en acide gluconique de 70 % en seulement 5 h (conversion à 99,9 % en  10h) de réaction au lieu de 30 % après plus de 20 h de réaction en système non aéré. L’agitation s’est également avéré être un paramètre important, celle-ci permettant l’homogénéisation du milieu ainsi qu’une meilleure dispersion de l’O2. Une réaction a ensuite été réalisée dans les conditions optimales mais en l’absence de l’enzyme pour vérifier l’absence d’oxydation résiduelle due à l’apport important d’oxygène. Il en ressort que la réaction a besoin de l’abaissement de sa barrière énergétique par l’enzyme pour avoir lieu. Enfin, la spécificité de l’enzyme a été testée (absence de réactions croisées) sur des sucres de structure proche. Le maltose, cellobiose et les maltooligosaccharides ne sont pas oxydés ou sont oxydés à des vitesses telles que la réaction n’a pas lieu dans nos conditions. Seul le saccharose est oxydé et donne différents acides dont l’acide gluconique. Enfin, l’acide gluconique peut être purifié de ses impuretés (glucose et maltooligosaccharides résiduels) par rétention sur résine échangeuse d’anions (forme AcO-). Dans un premier temps, la séparation est réalisée avec un passage unique sur résine (100mg de résine par ml de solution). Néanmoins, une large proportion de l’acide gluconique est perdu dans l’éluat et les deux lavages alors qu’à peine 0,2% de glucose sont retrouvés au niveau de la fraction d’élution de la résine au HCl. Lors d’une seconde purification, l'utilisation d'une plus grande quantité de résine (200 mg/ml) et le passage des éluats sur une seconde résine, pour pallier à la saturation de la première ont permis de réduire à 17 % (de la masse de départ) l’acide gluconique retrouvé dans l’éluat final et à nouveau d’obtenir une fraction AG très pauvre en glucose (0,022%). [less ▲]

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See detailProduction d’arômes de type lactone par des levures.
Alchihab, Mohamed ULg; Destain, Jacqueline ULg; Aguedo, Mario ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(4), 681-691

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See detailProduction d'énergie et environnement
Nicolas, Jacques ULg

Report (1993)

Fondation Universitaire Luxembourgeoise was involved in a study supported by European Commission to analyse the various possibilities of energy production in Rio Grande do Sul, in Bresil. The present ... [more ▼]

Fondation Universitaire Luxembourgeoise was involved in a study supported by European Commission to analyse the various possibilities of energy production in Rio Grande do Sul, in Bresil. The present report is a preliminary overview of the pro and con of different energy production systems on the environmental point of view. [less ▲]

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See detailProduction d'esquisses créatives en conception digitale
Leclercq, Pierre ULg; Mayeur, A.; Darses, F.

in Agon, C.; Delerue, O. (Eds.) Proceedings of the 19th International Conference of the Association Francophone d'Interaction Homme-Machine, IHM (2007)

Detailed reference viewed: 27 (2 ULg)