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See detailMolecular adaptations of alpha-amylase from psychrophilic bacteria
Feller, Georges ULg; LIBIOULLE, Cécile ULg; Payan, Françoise et al

Poster (1995)

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See detailMolecular adaptations of alpha-amylase from psychrophilic bacteria
Feller, Georges ULg; LIBIOULLE, Cécile ULg; Payan, Françoise et al

Poster (1994)

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See detailMolecular adaptations of alpha-amylase from psychrophilic bacteria
Feller, Georges ULg; LIBIOULLE, Cécile ULg; Payan, Françoise et al

Poster (1994)

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See detailMolecular adaptations of alpha-amylase from psychrophilic bacteria.
Feller, Georges ULg; LIBIOULLE, Cécile ULg; Payan, Françoise et al

Poster (1994)

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See detailMolecular adaptations of cold enzymes
Feller, Georges ULg

Conference (1998)

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See detailMolecular adaptations of enzymes from psychrophilic bacteria
Feller, Georges ULg

Conference (1995)

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See detailMolecular adaptations of enzymes from psychrophilic organisms
Feller, Georges ULg; Arpigny, J. L.; Narinx, E. et al

in Comparative Biochemistry and Physiology. A : Comparative Physiology (1997), 118(3), 495-499

The dominating adaptative character of enzymes from cold-evolving organisms is their high turnover number (k(cat)) and catalytic efficiency (k(cat)/K-m), which compensate for the reduction of chemical ... [more ▼]

The dominating adaptative character of enzymes from cold-evolving organisms is their high turnover number (k(cat)) and catalytic efficiency (k(cat)/K-m), which compensate for the reduction of chemical reaction rates inherent to low temperatures. This optimization of the catalytic parameters can originate from the highly flexible structure of these proteins providing enhanced abilities to undergo conformational changes during catalysis at low temperatures. Molecular modelling of the 3-D structure of cold-adapted enzymes reveals that only subtle modifications of their conformation can be related to the structural flexibility. The observed structural features include: 1) the reduction of the number of weak interactions involved in the folded state stability like salt bridges, weakly polar interactions between aromatic side chains, hydrogen bonding, arginine content and charge-dipole interactions in alpha-helices; 2) a lower hydrophobicity of the hydrophobic clusters forming the core of the protein; 3) deletion or substitution of proline residues in loops or turns connecting secondary structures; 4) improved solvent interactions with a hydrophilic surface via additional charged side chains; 5) the occurence of glycine clusters close to functional domains; and 6) a looser coordination of Ca2+ ions. No general rule from the molecular changes observed; rather, each enzyme adopts its own strategy by using one or a combination of these altered interactions. Enzymes from thermophiles reinforce the same type of interactions indicating that there is a continuity in the strategy of protein adaptation to temperature. (C) 1997 Elsevier Science Inc. [less ▲]

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See detailMolecular adaptations of enzymes from thermophilic and psychrophilic organisms
Arpigny, J. L.; Feller, Georges ULg; Davail, S. et al

in Giles, R. (Ed.) Advances in Comparative and Environmental Physiology Vol. 20 (1994)

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See detailMolecular adaptations to cold in psychrophilic enzymes
Feller, Georges ULg

in Cellular and Molecular Life Sciences : CMLS (2003), 60(4), 648-662

Psychrophiles or cold-loving organisms successfully colonize cold environments of the Earth's biosphere. To cope with the reduction of chemical reaction rates induced by low temperatures, these organisms ... [more ▼]

Psychrophiles or cold-loving organisms successfully colonize cold environments of the Earth's biosphere. To cope with the reduction of chemical reaction rates induced by low temperatures, these organisms synthesize enzymes characterized by a high catalytic activity at low temperatures associated, however, with low thermal stability. Thanks to recent advances provided by Xray crystallography, protein engineering and biophysical studies, we are beginning to understand the molecular adaptations responsible for these properties which appear to be relatively diverse. The emerging picture suggests that psychrophilic enzymes utilize an improved flexibility of the structures involved in the catalytic cycle, whereas other protein regions if not implicated in catalysis may or may not be subjected to genetic drift. [less ▲]

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See detailMolecular Alterations and Everolimus Efficacy in Human Epidermal Growth Factor Receptor 2-Overexpressing Metastatic Breast Cancers: Combined Exploratory Biomarker Analysis From BOLERO-1 and BOLERO-3.
Andre, Fabrice; Hurvitz, Sara; Fasolo, Angelica et al

in Journal of clinical oncology : official journal of the American Society of Clinical Oncology (2016), 34(18), 2115-24

PURPOSE: Two recent phase III trials, BOLERO-1 and BOLERO-3 (Breast Cancer Trials of Oral Everolimus), evaluated the addition of everolimus to trastuzumab and chemotherapy in human epidermal growth factor ... [more ▼]

PURPOSE: Two recent phase III trials, BOLERO-1 and BOLERO-3 (Breast Cancer Trials of Oral Everolimus), evaluated the addition of everolimus to trastuzumab and chemotherapy in human epidermal growth factor receptor 2-overexpressing advanced breast cancer. The current analysis aimed to identify biomarkers to predict the clinical efficacy of everolimus treatment. METHODS: Archival tumor samples from patients in BOLERO-1 and BOLERO-3 were analyzed using next-generation sequencing, immunohistochemistry, and Sanger sequencing. RESULTS: Biomarker data were available for 549 patients. PIK3CA activating mutations and PTEN loss were reported in 30% and 16% of BOLERO-1 samples and in 32% and 12% of BOLERO-3 samples, respectively. PI3K pathway was hyperactive (PIK3CA mutations and/or PTEN loss and/or AKT1 mutation) in 47% of BOLERO-1 and 41% of BOLERO-3 samples. In both studies, differential progression-free survival (PFS) benefits of everolimus were consistently observed in patient subgroups defined by their PI3K pathway status. When analyzing combined data sets of both studies, everolimus was associated with a decreased hazard of progression in patients with PIK3CA mutations (hazard ratio [HR], 0.67; 95% CI, 0.45 to 1.00), PTEN loss (HR, 0.54; 95% CI, 0.31 to 0.96), or hyperactive PI3K pathway (HR, 0.67; 95% CI, 0.48 to 0.93). Patients with wild-type PIK3CA (HR, 1.10; 95% CI, 0.83 to 1.46), normal PTEN (HR, 1.00; 95% CI, 0.80 to 1.26), or normal PI3K pathway activity (HR, 1.19; 95% CI, 0.87 to 1.62) did not derive PFS benefit from everolimus. CONCLUSION: This analysis, although exploratory, suggests that patients with human epidermal growth factor receptor 2-positive advanced breast cancer having tumors with PIK3CA mutations, PTEN loss, or hyperactive PI3K pathway could derive PFS benefit from everolimus. [less ▲]

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See detailMolecular alterations in breast cancers : deregulation of c-erbB2 gene expression.
Gol-Winkler, Rose; Pasleau, Françoise ULg; Grooteclaes, Madeleine et al

Conference (1993, September)

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See detailMolecular analysis and mating behaviour of the Trichophyton mentagrophytes species complex
Symoens, F.; Jousson, O.; Planard, C. et al

in International Journal of Medical Microbiology (2011), 301(3), 260-266

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See detailMolecular analysis for hybrid detection between European mink (Mustela lutreola) and polecat (Mustela putorius)
Cabria, Maria Teresa; Gomez-Moliner, Benjamin; Zardoya, Rafael et al

in abstract book of the 7th Baltic Theriological conference (2008)

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See detailMolecular Analysis in Two Siblings African Patients with Severe Form of Hunter Syndrome: Identification of a Novel (P.Y54x) Nonsense Mutation
Mutesa, Léon; Muganga, N.; Lissens, Willy et al

in Journal of Tropical Pediatrics (2007), 53(6), 434-7

Hunter syndrome (or Mucopolysaccharidosis type II, MPS II) is an X-linked recessive disorder due to the deficiency of the iduronate-2-sulfatase (IDS) enzyme, resulting in the accumulation of heparan and ... [more ▼]

Hunter syndrome (or Mucopolysaccharidosis type II, MPS II) is an X-linked recessive disorder due to the deficiency of the iduronate-2-sulfatase (IDS) enzyme, resulting in the accumulation of heparan and dermatan sulfates in the lysosomes. The heterogeneity of clinical phenotypes, ranging from mild-to-severe forms, is a result of different mutations in the IDS gene. We report here, a novel nonsense mutation (p.Y54X) in two siblings MPS II African patients affected with a severe form of the disease. We postulated that the p.Y54X mutation which causes a loss of the IDS region highly conserved among sulfatase enzymes, could be predicted as a severe disease-causing mutation for Hunter syndrome. [less ▲]

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See detailMolecular analysis of interaction between fluorescent Pseudomonads and Pythium spp
Jacques, Philippe ULg; Mistry, Ch.; Ongena, Marc ULg et al

Poster (1992, July)

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See detailMolecular analysis of miRNA expression profiles in AIP mutation positive somatotropinomas
Falk, N; Daly, Adrian ULg; Beckers, Albert ULg et al

in Endocrine Abstracts (2015, May)

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See detailMolecular analysis of root medium impact on Arabidopsis thaliana development
Bouché, Frédéric ULg; André, Julie; Tocquin, Pierre ULg et al

Poster (2013, April 18)

Hydroponics and soil are the most common media used for plant growth. Hydroponics has the main advantage of providing easy access to the root system and is therefore commonly used for gene expression ... [more ▼]

Hydroponics and soil are the most common media used for plant growth. Hydroponics has the main advantage of providing easy access to the root system and is therefore commonly used for gene expression analyses in molecular studies of the model plant Arabidopsis thaliana. However, the impact of root substrate on plant growth remains poorly documented. Here we show that hydroponics accelerates both shoot growth and developmental phases as compared with culture on soil. In order to identify molecular changes in the roots that could account for these medium effects, a transcriptomic comparison was performed by microarray analysis. This experiment revealed that more than 20% of the genes were differentially expressed in hydroponics vs soil. Among them, the flowering time gene FLOWERING LOCUS C and two clades of microRNA targeted genes. To further assess the role of these genes in roots, artificial microRNAs were designed for root specific expression in transgenic Arabidopsis plants. [less ▲]

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See detailMolecular analysis of the FOLR genes in patients with cerebral folate deficiency
SEGERS, Karin ULg; Hanson, J; RAMAEKERS, Vincent ULg et al

Poster (2010, November 02)

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See detailMolecular analysis of the interfacial and membrane-interacting properties of D-xylose-based bolaforms
Nasir, Mehmet Nail ULg; Legrand, Vincent; Gatard, Sylvain et al

Poster (2012, October)

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See detailMolecular Analysis Of The Ycr-242 Gene Encoding The Putative Mitochondrial Asparaginyl-Transfer-Rna Synthetase From Saccharomyces-Cerevisiae
Landrieu, I.; Vandenbol, Micheline ULg; Portetelle, Daniel ULg et al

in Archives Internationales de Physiologie de Biochimie et de Biophysique (1994), 102(2),

Detailed reference viewed: 16 (1 ULg)