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See detailPregnancy-associated glycoprotein detection by RIA in pregnant buffalo cows (Bubalus bubalis)
Debenedetti, A.; Melo de Sousa, Noelita ULg; Sulon, Joseph ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2001), 5(suppl), 62

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See detailPregnancy-Associated Glycoprotein E-55 kDa [Fragment] from Bison bonasus placenta - Access number P85323
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein E-65 kDa [Fragment] from Bubalus bubalis placenta - Access number P86376
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein F-50 kDa [Fragment] from Bison bonasus placenta - Access number P85324
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein F-58 kDa [Fragment] from Bubalus bubalis placenta - Access number P86377
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein G-71 kDa [Fragment] from Bison bonasus placenta - Access number P85325
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein G-76 kDa [Fragment] from Bubalus bubalis placenta - Access number P86378
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein H-60 kDa [Fragment] from Bison bonasus placenta - Access number P85326
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein H-63 kDa [Fragment] from Bubalus bubalis placenta - Access number P86379
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein I-50 kDa [Fragment] from Bison bonasus placenta - Access number P85327
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein I-73 kDa [Fragment] from Bubalus bubalis placenta -Access number P86371
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein J-63 kDa [Fragment] from Bubalus bubalis placenta - Access number P86370
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein K-60 kDa [Fragment] from Bubalus bubalis placenta - Access number P83369
Barbato, O.; Melo de Sousa, Noelita ULg; Barile, V. L. et al

E-print/Working paper (2009)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bubalus bubalis. Proteins were submitted to SwissProt databank.

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See detailPregnancy-associated glycoprotein levels in cattle exhibiting prolonged pregnancy: case reports.
Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg

in Proceedings of the 6th European Congress of Bovine Health and Management (ECBHM) (2011, September 07)

Prolonged gestation (PG) is a complex clinical entity which occurs in bovine species. This pathology has been described in Belgian Blue cattle. PG may require termination by cesarean section. Fetuses ... [more ▼]

Prolonged gestation (PG) is a complex clinical entity which occurs in bovine species. This pathology has been described in Belgian Blue cattle. PG may require termination by cesarean section. Fetuses often exhibit increased body weight, long hair and well erupted teeth. All calves die within minutes or hours after birth. From endocrinological point of view, PG is highly correlated to fetal adenohypophyseal aplasia. The aim of the present study was to investigate the usefulness of PAG RIA for diagnosis of prolonged gestation in Belgian Blue cattle. Blood samples were collected from five Blue Belgian cows having an abnormally long pregnancy length (10 to 15 months). PAG was measured by routine RIA with the use of antiserum AS497 as primary antibody. A purified bovine PAG preparation (boPAG67kDa) was used as standard (0.8 to 100 ng/mL) and for radio-iodination (125I). Cesarean section was used for termination of pregnancy of those cows exhibiting abnormal PAG concentrations. Morphological aspect and viability of fetuses were reported. Concentrations of PAG were very low (94.1 ng/mL, 50 ng/mL, 5 ng/mL, 110 ng/mL and 26 ng/mL) in the five females suffering of prolonged gestation. Fetuses issued from prolonged gestation exhibited long hair, well erupted teeth and died within few minutes after birth. In conclusion, PAG concentration is a good indicator of prolonged gestation in cattle. Concentrations are much lower than those expected in normal ongoing pregnancy (<120 ng/mL versus >1,000 ng/mL). [less ▲]

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See detailPregnancy-associated glycoprotein levels in pregnant goats inoculated with Toxoplasma gondii or Listeria monocytogenes: a retrospective study
Zarrouk, A.; Engeland, I. V.; Sulon, Joseph ULg et al

in Theriogenology (1999), 52(6), 1095-104

The pregnancy-associated glycoprotein (PAG) concentration profiles of goats that had been experimentally inoculated with either Toxoplasma gondii or Listeria monocytogenes are described. All goats were ... [more ▼]

The pregnancy-associated glycoprotein (PAG) concentration profiles of goats that had been experimentally inoculated with either Toxoplasma gondii or Listeria monocytogenes are described. All goats were examined regularly by ultrasonography. In T. gondii-infected females (n = 5), a slow decrease of PAG was observed throughout a period of 55 to 74 d after inoculation. Afterwards, the goats either aborted (n = 4) or kidded 1 dead and 1 weak fetus (n = 1). In L. monocytogenes-infected females (n = 8), a marked decrease of PAG was observed from the day of inoculation. Abortion occurred within 9 to 11 d post inoculation (n = 7). Only 1 goat kidded a healthy fetus. [less ▲]

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See detailPregnancy-associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows.
El-Battawy, K. A.; Melo de Sousa, Noelita ULg; Szenci, Otto et al

in Reproduction in Domestic Animals (2009), 44(2), 161166

Pregnancy-associated glycoprotein (PAG) concentrations were measured in buffalo cows starting from day 28 after breeding. Oestrus was synchronized in 10 buffaloes using two injections of 25 mg ... [more ▼]

Pregnancy-associated glycoprotein (PAG) concentrations were measured in buffalo cows starting from day 28 after breeding. Oestrus was synchronized in 10 buffaloes using two injections of 25 mg prostraglandin (PG)F(2alpha) (Lutalyse((R))) at a 11-day interval. Blood sampling was conducted nearly twice weekly. Results indicated that plasma PAG concentrations in non-pregnant buffaloes were low (<0.20 ng/ml) during the whole experimental period (day 28 to 103), while in pregnant animals plasma PAG levels increased from day 28 (4.48 +/- 0.92 ng/ml) until day 41 (27.27 +/- 6.74 ng/ml), remaining high (20.71 +/- 9.20 ng/ml) until day 103. Progesterone levels were significantly (p < 0.0001) higher in pregnant (3.51-4.80 ng/ml) than in non-pregnant buffaloes (0.28-1.52 ng/ml). A significant difference (p < 0.0001) in plasma PAG concentrations between pregnant and non-pregnant animals starting at day 28 after breeding suggests that PAG-radioimmunoassay could be suitable for pregnancy diagnosis in buffaloes during this period. In conclusion, PAG test offers the advantages that it requires a single plasma sample for early pregnancy diagnosis as well as the accuracy of the test for the detection of pregnancy as early as day 28. [less ▲]

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See detailPregnancy-associated glycoprotein profiles of 5 heifers measured by three radioimmunoassay systems.
Perenyi, Z.; Desbuleux, H.; Sulon, J. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 6-7

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See detailPregnancy-associated glycoprotein secretion in North Moroccan goats
Chentouf, M.; El Amiri, B.; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2008), 43(6), 696-700

The plasma profile of pregnancy-associated glycoprotein (PAG) and its relationship with fetal number were studied in 14 North Moroccan goats using a semi-heterologous radioimmunoassay (RIA). Peripheral ... [more ▼]

The plasma profile of pregnancy-associated glycoprotein (PAG) and its relationship with fetal number were studied in 14 North Moroccan goats using a semi-heterologous radioimmunoassay (RIA). Peripheral blood was collected every day during the first month of pregnancy, afterward the blood samples were collected trice a week. The PAG were first detected at day 20 of pregnancy, their levels increase to week 8 of gestation then decrease slightly until parturition. Statistical differences between goats carrying one or two foetuses are observed from week 5 of pregnancy until parturition. Factorial Discriminant Analysis provides mathematical models for the discrimination between the litter size using the PAG level and the week of pregnancy. Using only one blood sample per week, high sensitivity, specificity and overall accuracy (74%, 88% and 81%) were obtained using these models. It is concluded that the PAG RIA is an effective tool for early diagnosis of pregnancy and for discrimination between the litter sizes in North Moroccan goats. [less ▲]

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See detailPregnancy-associated glycoprotein, chymosin and pepsinogen immunoreactivity of proteins extracted from fetal gastric tissue in bovine species.
Bella, Amina ULg; Melo de Sousa, Noelita ULg; Dehimi, M. L. et al

in Research in Veterinary Science (2012), 92(3), 378-386

The objective of this work was to investigate the expression of gastric aspartic proteinases in fundic and pyloric mucosa removed from bovine fetuses. For this purpose, fractions issued from classical ... [more ▼]

The objective of this work was to investigate the expression of gastric aspartic proteinases in fundic and pyloric mucosa removed from bovine fetuses. For this purpose, fractions issued from classical biochemical protocols were analyzed by proteolytic method, by PAG-RIA and by Western blot with the use of antisera raised against both pepsinogens and PAG. A strong reaction of proteins extracted from the fundic mucosa collected at the beginning of pregnancy was revealed with both anti-bPAG-I and anti-bPAG-II antisera, suggesting the expression of pepsinogen F in bovine species. Concerning pyloric mucosa, the analysis by Western blot highlighted a very strong immunoreaction with the anti-bovine chymosin serum. Amino-terminal sequencing allowed to identify bovine fetuin and albumin in fundic extracts, chymosin in the pyloric mucosa extracts, as well as some unknown proteins in both mucosa. Despite no N-terminal microsequence corresponding to the hypothetical pepsinogen F could be identified, it cannot be excluded that an existing bovine pepsinogen F-like molecule could be degraded during the purification procedure or that co-purified proteins could be responsible for masking its N-terminal microsequence. [less ▲]

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See detailPregnancy-Associated Glycoproteins (Pag) as Pregnancy Markers in the Ruminants
Melo de Sousa, Noelita ULg; Ayad, A.; Beckers, Jean-François ULg et al

in Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society (2006), 57(Suppl 8), 153-71

In the last years, a polymorphic family of placenta-expressed proteins has been discovered in ruminant species and used for pregnancy diagnosis. Pregnancy diagnosis is an important part in reproduction ... [more ▼]

In the last years, a polymorphic family of placenta-expressed proteins has been discovered in ruminant species and used for pregnancy diagnosis. Pregnancy diagnosis is an important part in reproduction management of ruminants. The pregnancy-associated glycoproteins (PAG) are synthesized in the mono- and binucleate cells of the ruminant's trophectoderm. Part of them is released into maternal blood circulation where they can be assayed by different RIA and ELISA systems. Due to large variety of expressed molecules and to large variations in the post-translational processing of the glycoproteins, different immuno-systems present different ability to quantify the PAG released in blood. Recent investigations showed that surprisingly the level of milk production in ruminants can modify the concentration of PAG circulating in blood. On the whole, the data show that the RIA methods are very precise for measuring PAG concentrations in the maternal blood and milk of the ruminants. Different studies clearly indicate that milk can be used for pregnancy diagnosis in small ruminants. The sensitivity and specificity of this method are very high. The results showed the possibility of the use PAG in milk and in blood as pregnancy test. It is especially helpful in the diagnosis of gestation and in detection of embryonic mortality as a non stressed method in the pregnancy management in the ruminants. [less ▲]

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