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See detailPregnancy-associated glycoproteins in sheep: breeds, techniques and achievements
El Amiri, Bouchra; Melo de Sousa, Noelita ULg; Szenci, Otto et al

in Proceedings of the 23rd International Congress of Hungarian Association for Buiatrics (2013)

The aim of the present review is to summarize the achievements on pregnancy-associated glycoproteins in sheep during the last 20 years on terms of results obtained after purifications, molecular biology ... [more ▼]

The aim of the present review is to summarize the achievements on pregnancy-associated glycoproteins in sheep during the last 20 years on terms of results obtained after purifications, molecular biology, radio-immunoassays (RIA), enzyme linked immune sorbent assay (ELISA), double radial immune-diffusion (Ouchterlony) and Western blot techniques. The analysis of documented papers that concerned these attempts showed that the animal genetic material used was composed of variable breeds from north Africa, Europe and USA (the Aragonesa, Ouled Djellal, Laucaun drairy sheep, Berrichone, Texel x Norvegian crossed ewes, Awassi x Merino, Blackheaded Moutton sheep x Blackheaded German Mutton sheep (BGM), Rhoen sheep crosses of BGM with Dorper sheep, Assaf, Chura, Merino, Texel, Suffolk, Boujaâd Moroccan breed). The RIA represents the wildspread technique used (54%) while Oucherlony and Western blot had been limited in use (4% each). From 1990 to 2000 only 27% of the achievements being available today were realized. The main data, profiles and studies were performed from 2000 to now (63%). To conclude, this review allows summarizing and updating the knowledge about the PAG in sheep. [less ▲]

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See detailThe pregnancy-associated glycoproteins in sheep: new investigations by using the Ouchterlony technique.
El Amiri, B.; Garbayo, J. M.; Mecif, K. et al

in Proceedings of the 15th Scientific Meeting of the European Embryo Transfer Association (1999)

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See detailPregnancy-associated glycoproteins in the ruminants: inactive members of the Aspartic Protease family
Beckers, Jean-François ULg; Drion, Pierre ULg; Garbayo, J. M. et al

in Proceedings of the Middel European Buiatrics Congress, 1998 (1998, May 21)

Proteins secreted by the placenta, when detected in the peripheral circulation of the mother, can be useful indicators of both pregnancy and feto-trophoblast well-being (1-3). In 1982, Butler et al. (4 ... [more ▼]

Proteins secreted by the placenta, when detected in the peripheral circulation of the mother, can be useful indicators of both pregnancy and feto-trophoblast well-being (1-3). In 1982, Butler et al. (4) isolated two pregnancy-specific proteins (PSPA and PSPB) from bovine placental membranes. PSPA was identified as a-fetoprotein which is not strictly limited to pregnancy, while PSPB was confirmed as placenta’s and pregnancy’s specific (5). PSPB was characterized as a glycoprotein showing relative molecular masses (Mr) between 47 and 53 kDa and presenting different isoelectric points (from 4.0 to 4.4). The Mr of PSPB was similar to the Mr of the molecule isolated by Laster in 1977 (6). In 1991, Zoli et al. (7) purified a pregnancy-associated glycoprotein (PAG), later designated PAG_1 and presently designated as PAG_I_ 67 considering its Mr. When isolated from bovine fetal cotyledons PAG_I_67 was an acidic glycoprotein of 67 kDA. Four isoforms (PI : 4.4, 4.6, 5.2 and 5.4) were detected in the initial preparation. Later molecular cloning studies showed that the PSPB and PAG_I_67 were closely related in primary structure (8). These glycoproteins (either PSPB and PAG_I_67) could be detected in the maternal circulation at around the time that the trophoblast formed definitive attachment to the uterine wall (figures A & B). Concentrations increased gradually therefore and reached peak values just before parturition at about 1 to 5 mg/ml (9).The PSPB and PAG molecules are routinely determined in peripheral maternal blood as pregnancy markers in cattle (5, 9,10). Glycoproteins immunologically related to PAG_I_67 and PSPB have been isolated and partially characterized from ovine fetal cotyledons: oPAG later designated oPAG_I (11) and oPSPB (12). They also have been detected in maternal blood by week third (12) or fourth (13) after breeding. Different forms (differing in Mr and isoelectric point) were characterized after isolation from sheep cotyledons cultured in vitro (14). Very recently, 3 different PAGs were characterized from goat placenta having Mr of 55, 59 and 62 kDa. Each of them presented various isoeletric points (15). In 1991, Xie et al., cloned PAG (now known as PAG_I_67) from late bovine and ovine placenta by screening cDNA libraries with two anti-PAG antisera (16). The bovine and ovine cDNAs encoding PAG_I shared 86% nucleotide sequence identic with one and other and encoded proteins of 380 and 382 aminoacids respectively including a 15 aminoacid signal sequence. However, protein sequence data, (peptidic sequencing) already showed the first aminoacid of the bovine PAG_I_67 was an arginine that corresponded to another one located at position 39, downstream of the side of signal sequence cleavage, indicating that PAG_I_67 undergoes post-translational modifications from a proform. [less ▲]

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See detailPregnancy-associated glycoproteins isolated from ewe placenta
El Amiri, B; Remy, B; Melo de Sousa, Noelita ULg et al

in Proceeding of pregnancy-associated glycoproteins isolated from ewe placenta (2002, September 06)

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See detailPregnancy-associated glycoproteins isolated from the ewe placenta removed at two different stages of gestation.
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

in Proceedings of the 7e Journée de Rencontre Bioforum (2002)

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See detailThe pregnancy-associated glycoproteins. Clinical applications for pregnancy follow-up in ruminant species.
Barbato, O.; Melo de Sousa, Noelita ULg; Canali, C. et al

in Revista română de medicină veterinară (2011), 21(2), 117-126

Pregnancy diagnosis is an important part in reproduction management of ruminant and embryonic mortality has a substantial impact on the fertility of demestic animals. This article focus on the use of ... [more ▼]

Pregnancy diagnosis is an important part in reproduction management of ruminant and embryonic mortality has a substantial impact on the fertility of demestic animals. This article focus on the use of pregnancy-associated glycoproteins for pregnancy follow-up. [less ▲]

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See detailThe Pregnancy-Associated Glycoproteins: Biochemical Aspects and Clinical Application for Pregnancy Follow-up
Melo de Sousa, Noelita ULg; Barbato, O.; Bella, Amina ULg et al

Conference (2007)

Pregnancy diagnosis is an important part in reproduction management of ruminants. In the last years, a large polymorphic family of placenta-expressed proteins has been discovered in ruminant species and ... [more ▼]

Pregnancy diagnosis is an important part in reproduction management of ruminants. In the last years, a large polymorphic family of placenta-expressed proteins has been discovered in ruminant species and used for pregnancy diagnosis. Members of this family are named pregnancy-associated glycoproteins (PAG), being synthesized in the mono- and binucleate cells of the ruminant’s trophectoderm. Part of them are released in the maternal blood circulation where they can be assayed by different laboratory techniques. Due to large variety of expressed molecules and to large variations in the post-translational processing of the PAG, different immuno-systems present different ability to quantify the PAG released in blood. The sensitivity (92 to 100%) and specificity of PAG radioimmunoassay when used for pregnancy diagnosis are very high. The assay of PAG can also bring very interesting information for researchers working in programs focused on the study of embryonic and fetal mortalities, as well as on embryo biotechnology (ET, FIV, clonage), animal nutrition, or infections diseases resulting in pathologies affecting the pregnancy. [less ▲]

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See detailPregnancy-associated proteins isolated from ewe placenta.
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

in Proceedings of the 18th Scientific Meeting of the European Embryo Transfer Association (2002)

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See detailPrehensile wear on flint tools
Rots, Veerle ULg

in Lithic Technology (2004), 29(1), 7-32

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See detailPrehension and Hafting Traces on Flint Tools. A Methodology
Rots, Veerle ULg

Book published by Leuven University Press (2010)

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See detailLa préhistoire
Otte, Marcel ULg; Noiret, Pierre ULg; Plumet, Patrick et al

Book published by De Boeck - 3ème éd. (2009)

L'ouvrage retrace la préhistoire de l'humanité, dans ses modifications anatomiques, spirituelles et comportementales , depuis les origines il y a 3 millions d'années, jusqu'aux derniers peuples prédateurs ... [more ▼]

L'ouvrage retrace la préhistoire de l'humanité, dans ses modifications anatomiques, spirituelles et comportementales , depuis les origines il y a 3 millions d'années, jusqu'aux derniers peuples prédateurs, voici environ dix mille ans. Les aspects techniques, économiques, sociaux et religieux sont évoqués successivement dans leurs contextes propres. Toutes les valeurs qui constituent notre mode de vie actuel se sont lentement formées durant ce processus et la succession de leurs agencements en donne ici leur signification et leur portée réelles. Un chapitre particulier est consacré au développement de l'art paléolithique européen; un autre présente la préhistoire des civilisations américaines. [less ▲]

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See detailPrehistoric art analysis, from the laboratory to in-situ measurement
Beck, Lucile; Menu, Michel; Laval, Eric et al

Scientific conference (2010)

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See detailPrehistorische technologie en modern gedrag
Rots, Veerle ULg

in Karakter. Tijdschrift van Wetenschap (2010), 32

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See detailLe préjudice lié à la personne soustrait à l'emprise du règlement collectif de dettes, note sous Cour cons., 2 oct. 2008
Georges, Frédéric ULg

in Revue de Jurisprudence de Liège, Mons et Bruxelles (2008)

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See detailPréjugés et maladie d'Alzheimer
Adam, Stéphane ULg

Conference given outside the academic context (2012)

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See detailPrélèvement d'organes après euthanasie: expérience belge
Ysebaert, Dirk; Detry, Olivier ULg; Squifflet, Jean-Paul ULg et al

Conference (2008, October 10)

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See detailPrélèvement de l’isthme encéphalique pour la recherche de l’encéphalopathie spongiforme transmissible chez le mouton
Gabriel, Annick ULg; Jacobs, Christine ULg; Simoens, P.

in Annales de Médecine Vétérinaire (2002), 146(5), 295-300

Depuis le premier avril 2002, une directive de l'Union Européenne contraint la Belgique à tester les chèvres et les moutons, en plus des bovins, dans le cadre de la recherche d'encéphalopathie spongiforme ... [more ▼]

Depuis le premier avril 2002, une directive de l'Union Européenne contraint la Belgique à tester les chèvres et les moutons, en plus des bovins, dans le cadre de la recherche d'encéphalopathie spongiforme transmissible. Comme chez les bovins, le diagnostic est réalisé à partir d'un prélèvement de l'isthme encéphalique au niveau de l'obex. Des différences morphologiques entre l'isthme encéphalique des moutons et chèvres et celui des bovins ont nécessité quelques ajustements de la méthode de prélèvement. Dans cette étude, les particularités techniques du prélèvement et certaines caractéristiques morphologiques du cerveau ovin sont décrites. Les différences majeures, par rapport aux bovins, sont d'une part les dimensions plus petites, et d'autre part le rétrécissement caudal très marqué de l'isthme encéphalique. [less ▲]

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See detailPrélèvement et rôle du laboratoire
MELIN, Pierrette ULg

Conference (1996, February 10)

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