Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailMethods to Assess the Activation of the Alternative (Noncanonical) NF-kappaB Pathway by Non-death TNF Receptors.
Remouchamps, Caroline ULg; Dejardin, Emmanuel ULg

in NF-kappa B: Methods and Protocols (2015)

The alternative or noncanonical NF-kappaB pathway regulates the generation of p52-containing NF-kappaB dimers (e.g., p52/RelB) through a partial degradation (called processing) of the precursor p100 into ... [more ▼]

The alternative or noncanonical NF-kappaB pathway regulates the generation of p52-containing NF-kappaB dimers (e.g., p52/RelB) through a partial degradation (called processing) of the precursor p100 into p52. This pathway is activated by a subset of non-death TNF receptor members, which ultimately activate two kinases: NIK (NF-kappaB-Inducing Kinase) and IKKalpha (Inhibitor of kappaB Kinase alpha). These kinases create a phosphodegron for the E3 ligase SCF-beta-TrCP that covalently binds K48-linked polyubiquitin chain onto p100 prior to its proteasomal processing. The resulting p52-containing complexes translocate into the nucleus to activate target genes involved in secondary lymphoid organ development, B cell survival or in osteoclastogenesis.We describe in this chapter straightforward methods to monitor the activation of the alternative NF-kappaB pathway. These methods uncover cytosolic and nuclear biochemical modifications of key proteins of the alternative NF-kappaB pathway required prior to the transcription of NF-kappaB target genes. [less ▲]

Detailed reference viewed: 27 (12 ULg)
Full Text
Peer Reviewed
See detailMethods to Increase the Robustness of Finite-Volume Flow Models in Thermodynamic Systems
Quoilin, Sylvain ULg; Bell, Ian ULg; Desideri, Adriano ULg et al

in Energies (2014), 7(3), 1621-1640

This paper addresses the issues linked to simulation failures during integration in finite-volume flow models, especially those involving a two-phase state. This kind of model is particularly useful when ... [more ▼]

This paper addresses the issues linked to simulation failures during integration in finite-volume flow models, especially those involving a two-phase state. This kind of model is particularly useful when modeling 1D heat exchangers or piping, e.g., in thermodynamic cycles involving a phase change. Issues, such as chattering or stiff systems, can lead to low simulation speed, instabilities and simulation failures. In the particular case of two-phase flow models, they are usually linked to a discontinuity in the density derivative between the liquid and two-phase zones. In this work, several methods to tackle numerical problems are developed, described, implemented and compared. In addition, methods available in the literature are also implemented and compared to the proposed approaches. Results suggest that the robustness of the models can be significantly increased with these different methods, at the price of a small increase of the error in the mass and energy balances. [less ▲]

Detailed reference viewed: 205 (29 ULg)
Full Text
Peer Reviewed
See detailMethods to study protein aggregation and amyloid formation
Dumoulin, Mireille ULg; Bader, R.

in Protein & Peptide Letters (2006), 13

Detailed reference viewed: 31 (3 ULg)
Full Text
See detailMethof for the elution of 18F fluoride trapped on an anion-exchange phase in a form suitable for efficient radiolabelling without any evaporation step
Aerts, Joël ULg; Lemaire, Christian ULg; Lignon, Steve et al

Patent (2008)

The invention relates to a method to ext. out of an aq. soln., conc. and​/or reformulate [18F] fluorides without any evapn. step, characterized in that the eluting soln. is a low water content, preferably ... [more ▼]

The invention relates to a method to ext. out of an aq. soln., conc. and​/or reformulate [18F] fluorides without any evapn. step, characterized in that the eluting soln. is a low water content, preferably <3​% water, org. soln. contg. at least: a first compd. (A) which is a tertiary alc.-​function bearing mol., a second compd. (B) which is a phase transfer agent suitable for radiolabeling and which is necessary to the anion exchange process. Said org. solvent is selected from acetonitrile (ACN)​, DMSO)​, dimethylacetamide, DMF, THF, di-​Et ether, dioxane, Et acetate, acetone, isobutyronitrile, benzonitrile, pyridine, di-​Et carbonate, sulfolane, hexamethylphosphotriamide (HMPA​/HMPT)​, and any mix of these solvents. Said nonpolar org. solvent is selected from pentane, hexane, heptane, octane, nonane, decane, and cyclohexane. [less ▲]

Detailed reference viewed: 14 (2 ULg)
Full Text
Peer Reviewed
See detailLe méthotrexate en rhumatologie: une révolution thérapeutique
Kaye, O.; Ribbens, Clio ULg; Hauwaert, Christian ULg et al

in Revue Médicale de Liège (1995), 50(11), 453-457

Detailed reference viewed: 121 (3 ULg)
Full Text
Peer Reviewed
See detailMethyl-laudanosine: A new pharmacological tool to investigate the function of small-conductance Ca2+-activated K+ channels
Scuvée-Moreau, Jacqueline ULg; Liégeois, Jean-François ULg; Massotte, Laurent ULg et al

in Journal of Pharmacology and Experimental Therapeutics (The) (2002), 302(3), 1176-1183

Small-conductance Ca(2+)-activated K(+) channels (SK channels) underlie the prolonged postspike afterhyperpolarization (AHP) observed in many central neurons and play an important role in modulating ... [more ▼]

Small-conductance Ca(2+)-activated K(+) channels (SK channels) underlie the prolonged postspike afterhyperpolarization (AHP) observed in many central neurons and play an important role in modulating neuronal activity. However, a lack of specific and reversible blockers of these channels hampers their study in various experimental conditions. Because previous work has shown that bicuculline salts block these channels, we examined whether related alkaloids, namely laudanosine quaternary derivatives, would produce similar effects. Intracellular recordings were performed on rat midbrain dopaminergic neurons and hippocampus CA1 pyramidal cells. Binding experiments were performed on rat cerebral cortex membranes. Laudanosine, methyl-laudanosine, and ethyl-laudanosine blocked the apamin-sensitive AHP of dopaminergic neurons with mean IC(50) values of 152, 15, and 47 microM, respectively. The benzyl and butyl derivatives were less potent. Methyl-laudanosine had no effect on the I(h) current, action potential parameters, or membrane resistance of dopaminergic cells, or on the decrease in input resistance induced by muscimol, indicating a lack of antagonism at GABA(A) receptors. Interestingly, 100 microM methyl-laudanosine induced a significant increase in spiking frequency of dopaminergic neurons but not of CA1 pyramidal cells, suggesting the possibility of regional selectivity. Binding experiments on laudanosine derivatives were in good agreement with electrophysiological data. Moreover, methyl-laudanosine has no affinity for voltage-gated potassium channels, and its affinity for SK channels (IC(50) 4 microM) is superior to its affinity for muscarinic (IC(50) 114 microM) and neuronal nicotinic (IC(50) > or =367 microM) receptors. Methyl-laudanosine may be a valuable pharmacological tool to investigate the role of SK channels in various experimental models. [less ▲]

Detailed reference viewed: 56 (20 ULg)
Full Text
Peer Reviewed
See detailMethylation analysis of the imprinted DLK1-GTL2 domain supports the random parental origin of the IGH-involving del(14q) in B-cell malignancies.
Katrincsakova, Beata; Takeda, Haruko ULg; Urbankova, Helena et al

in Epigenetics : Official Journal of the DNA Methylation Society (2009), 4(7), 469-75

Leukemias/lymphomas with IGH-involving del(14q)(1) commonly lose the DLK1-GTL2 imprinted domain that comprises several paternally and maternally expressed genes, including a cluster of microRNAs. Given ... [more ▼]

Leukemias/lymphomas with IGH-involving del(14q)(1) commonly lose the DLK1-GTL2 imprinted domain that comprises several paternally and maternally expressed genes, including a cluster of microRNAs. Given that deletion of this region could lead to inactivation of a monoallelically expressed tumor suppressor gene, our study aimed at determination of the parental origin of del(14q/IGH). The designed allele-specific methylation study of the DLK1/GTL2 intergenic differentially methylated region allowed us to determine the parental origin of del(14q/IGH) in 9/20 analyzed cases. In six cases del(14q/IGH) was of the paternal origin and in three cases of the maternal origin. These findings argue against the concept that a TSG/anti-oncomir located in the imprinted region is systematically inactivated by a targeted deletion of its functional allele. [less ▲]

Detailed reference viewed: 30 (3 ULg)
Full Text
Peer Reviewed
See detailMethylene blue: An old-timer or a compound ready for revival?
Donati, A.; Preiser, Jean-Charles ULg

in Critical Care Medicine (2006), 34(11), 2862-2863

Detailed reference viewed: 9 (0 ULg)
Full Text
See detailMethylmercury and selenium in vitro effects on harbor seal (Phoca vitulina) lymphocytes : a multidisciplinary approach
Dupont, Aurélie ULg; Bouquegneau, Jean-Marie ULg; Das, Krishna ULg et al

Poster (2010, May 26)

Methylmercury (MeHg) bioaccumulates along the food web, leading to the highest levels in tissues of predatory species. It constitutes the predominant form present in the blood of marine mammals. The blood ... [more ▼]

Methylmercury (MeHg) bioaccumulates along the food web, leading to the highest levels in tissues of predatory species. It constitutes the predominant form present in the blood of marine mammals. The blood cells, including the immune cells, are therefore exposed to the toxic properties of that chemical. Nevertheless, selenium (Se) is an essential element absorbed concomitantly to MeHg which seems to modulate this toxicity. The goal of this study is to evaluate the immunotoxicity of MeHg on the harbor seal (Phoca vitulina) T lymphocytes, highly important in the adaptive immune response, and to investigate the modulating effect of Se on that toxicity. In parallel, the concentrations of MeHg, total mercury (T-Hg) and Se are determined in free-ranging harbour seal blood in order to follow their contamination levels. The T lymphocytes were isolated from the whole blood, exposed to various MeHg and Se concentrations and the exposure effects were estimated by functional tests including the evaluation of viability, proliferation, metabolic activity, DNA and protein synthesis, and by morphological analysis by transmission electron microscopy. The mean T-Hg concentration was 172 ± 143 µg/l of whole blood. The T lymphocytes cultures in vitro displayed a decreasing number of viable cells with increasing concentrations of MeHg, and numerous ultrastructural defects. The cells exposed to MeHg notably displayed distortion of the plasmic membrane, nucleus fragmentations, chromatin compaction, swelling mitochondrias and cytoplasmic vacuolisations. Those results highlighted various immunotoxic effects of MeHg, both at the functional and ultrastructural levels. The antagonistic role of Se on MeHg immunotoxicity is discussed. [less ▲]

Detailed reference viewed: 107 (7 ULg)
Full Text
Peer Reviewed
See detailMethylmercury in vitro exposure of harbor seal (Phoca vitulina) lymphocytes: a multidisciplinary approach
Dupont, Aurélie ULg; Siebert, Ursula; Rosenberger, Tanja et al

Poster (2009, June 08)

Detailed reference viewed: 26 (2 ULg)
Full Text
Peer Reviewed
See detailUn métier à tisser thérapeutique
Triffaux, Jean-Marc ULg; Gayetot, Delphine ULg; Izzi, Isabelle

in Revue des Hôpitaux de Jour Psychiatriques et des Thérapies Institutionnelles (2008), 10

Detailed reference viewed: 43 (2 ULg)