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See detailMetabolomic analysis of Strychnos nux-vomica, Strychnos icaja and Strychnos ignatii extracts by H-1 nuclear magnetic resonance spectrometry and multivariate analysis techniques
Frederich, Michel ULg; Choi, Young Hae; Angenot, Luc ULg et al

in Phytochemistry (2004), 65(13), 1993-2001

H-1 Nuclear magnetic resonance spectrometry and multivariate analysis techniques were applied for the metabolic profiling of three Strychnos species: Strychnos nux-vomica (seeds, stem bark, root bark ... [more ▼]

H-1 Nuclear magnetic resonance spectrometry and multivariate analysis techniques were applied for the metabolic profiling of three Strychnos species: Strychnos nux-vomica (seeds, stem bark, root bark), Strychnos ignatii (seeds), and Strychnos icaja (leaves, stem bark, root bark, collar bark). The principal component analysis (PCA) of the H-1 NMR spectra showed a clear discrimination between all samples, using the three first components. The key compounds responsible for the discrimination were brucine, loganin, fatty acids.. and Strychnos icaja alkaloids such as icajine and sungucine. The method was then applied to the classification of several "false angostura" samples. These samples were, as expected, identified as S. nux-vomica by PCA, but could not be clearly discriminated as root bark or stem bark samples after further statistical analysis. (C) 2004 Published by Elsevier Ltd. [less ▲]

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See detailMetabolomic analysis of the green microalga Chlamydomonas reinhardtii cultivated under day/night conditions
Willamme, Rémi ULg; Alsafra, Zouheir; Arumugam, Rameshkumar et al

in Journal of Biotechnology (2015)

Detailed reference viewed: 68 (34 ULg)
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See detailMetabolomic and molecular signatures of Mascarene Aloes using a multidisciplinary approach
Govinden-Soulange, J.; Lobine, D.; Frederich, Michel ULg et al

in South African Journal of Botany (2017), 108

In this research a multidisciplinary approach was used to unveil the genetic, metabolic uniqueness and relationships of endemic Mascarene Aloes (Aloe macra, Aloe purpurea, Aloe tormentorii) with respect ... [more ▼]

In this research a multidisciplinary approach was used to unveil the genetic, metabolic uniqueness and relationships of endemic Mascarene Aloes (Aloe macra, Aloe purpurea, Aloe tormentorii) with respect to Aloe vera. Nuclear magnetic resonance spectroscopy, DNA sequencing and antioxidant profiles of these Aloes were studied. Principal component analysis following 1H NMR revealed the specificity of the Mascarene Aloes relative to Aloe vera. The superior free radical scavenging ability of A. purpurea, A. macra and A. tormentorii as compared to other Aloes was also unveiled. Phylogenetic analyses of chloroplast genes and ITS region sequences of these Mascarene Aloes were done using maximum parsimony and Bayesian analysis. Mascarene Aloes clustered within one clade separate from Aloe vera confirming their relative recent emergence in this genus. Results from this study showed that there is sufficient evidence at the metabolomic and molecular level to distinguish between Aloe ​purpurea from Mauritius and that of Reunion. © 2016 SAAB [less ▲]

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See detailMetabolomic investigation of the ethnopharmacological use of Artemisia afra with NMR Spectroscopy and Multivariate Data Analysis.
Liu, N. Q.; Cao, Martine ULg; Frederich, Michel ULg et al

in Journal of Ethnopharmacology (2010), 128

Ethnopharmacological relevance: Artemisia afra has been used as an infusion to treat malaria throughout the southern parts of Africa, in much the same way as the antimalarial plant Artemisia annua in ... [more ▼]

Ethnopharmacological relevance: Artemisia afra has been used as an infusion to treat malaria throughout the southern parts of Africa, in much the same way as the antimalarial plant Artemisia annua in China. The antiplasmodial activity of purified components from an apolar fraction of A. afra has been shown in the past. No data on the efficacy of the tea infusion prepared from A. afra are however available. OBJECTIVE: To investigate the antiplasmodial activity of various extracts of A. afra including an ethnopharmacological prepared sample. To identify polar metabolites in A. afra and A. annua and by using multivariate data analysis investigate the metabolic differences between these species. Materials and methods: The antiplasmodial activity of A. afra and A. annua extracts were tested for activity against Plasmodiam falciparum 3D7 (chloroquine-sensitive strain) with chloroquine, quinine and artemisinin as positive controls. Hydrophilic metabolites in A. afra and A. annua were identified directly from the crude extracts through 1D- and 2D-NMR spectra. The NMR spectra were also used to differentiate between the two species using principal component analysis (PCA) for quality control purposes. RESULTS: The apolar fractions of both A. afra and A. annua showed activity against P. falciparum while activity was only found in the tea infusion of A. annua. Metabolomic studies using 1D- and 2D-NMR spectroscopy identified 24 semi-polar components in A. afra including three new phenylpropanoids for this species: caffeic acid, chlorogenic acid and 3,5-dicaffeoyl quinic acid. PCA analysis conducted on the samples yielded good separation between the polar extracts of A. afra and A. annua. CONCLUSION: These findings shows that there are no in vitro activity in the tea infusion of A. afra and lists the identified metabolites causing the metabolic differences between A. afra and A. annua for quality control purposes. [less ▲]

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See detailMetabolomic, proteomic and preclinical imaging of patient-derived tumor xenografts for improving treatment of liver metastases patients
Perez Palacios, A; Blomme, A; Boutry, S et al

in Acta Gastro-Enterologica Belgica (2015, March), 78(1), 134

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See detailMetabolomics : a new insight into cells and organisms diseases
De Tullio, Pascal ULg

Conference (2010, October 25)

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See detailMetabolomics : Toward a better understanding of cells and organisms diseases
De Tullio, Pascal ULg

Conference (2010, October 08)

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See detailMetabolomics analysis of Galium odoratum (L.) Scop.: impact of the plant population origin and growth conditions.
Ledoux, Allison ULg; Martin, Bertrand; De Tullio, Pascal ULg et al

Poster (2015, July 16)

Galium odoratum is a plant used in traditional medicine and to prepare beverages. This work aimed at studying the impact of plant origin and growth conditions on the metabolite content of the plant ... [more ▼]

Galium odoratum is a plant used in traditional medicine and to prepare beverages. This work aimed at studying the impact of plant origin and growth conditions on the metabolite content of the plant. Material and methods- Aerial biomass of Galium odoratum was collected from five natural populations (in situ conditions) and from controlled environment (ex situ conditions). Results- Quantitative analysis of selected phytochemicals including phenylpropranoids and iridoids showed clear differences between the plants from nature and those of controlled growth conditions as well as internal variation within the group. The metabolomic approach emphasized the decrease of the secondary metabolites pool paralleled by an increase of the carbohydrates in ex situ conditions. Conclusion- Metabolomics approaches using 1H-NMR and HPLC is worth to consider for studying the impact of climate factors on the regulation of the phytochemical profile in relation to the origin of the plant material. [less ▲]

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See detailMetabolomics as a Challenging Approach for Medicinal Chemistry and Personalized Medicine.
Frederich, Michel ULg; Pirotte, Bernard ULg; Fillet, Marianne ULg et al

in Journal of Medicinal Chemistry (2016), 59(19), 86498666

"Omics" sciences have been developed to provide a holistic point of view of biology and to better understand the complexity of an organism as a whole. These systems biology approaches can be examined at ... [more ▼]

"Omics" sciences have been developed to provide a holistic point of view of biology and to better understand the complexity of an organism as a whole. These systems biology approaches can be examined at different levels, starting from the most fundamental, i.e., the genome, and finishing with the most functional, i.e., the metabolome. Similar to how genomics is applied to the exploration of DNA, metabolomics is the qualitative and quantitative study of metabolites. This emerging field is clearly linked to genomics, transcriptomics, and proteomics. In addition, metabolomics provides a unique and direct vision of the functional outcome of an organism's activities that are required for it to survive, grow, and respond to internal and external stimuli or stress, e.g., pathologies and drugs. The links between metabolic changes, patient phenotype, physiological and/or pathological status, and treatment are now well established and have opened a new area for the application of metabolomics in the drug discovery process and in personalized medicine. [less ▲]

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See detailMétabolomique - Identification de métabolites (biomarqueurs) par RMN 2D et LC-NMR
Frederich, Michel ULg

Conference (2009, January 28)

Detailed reference viewed: 59 (8 ULg)