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See detailPreference for and acceptability of two formulations of a dietary supplement containing calcium plus vitamin D-3: A randomized, open-label, crossover trial in adult patients with calcium and vitamin D deficiencies
Reginster, Jean-Yves ULg; Kaufman, J. M.; Gangjii, V.

in Current therapeutic Research-Clinical and Experimental (2005), 66(1, JAN-FEB), 23-34

Background: Preference for and acceptability of a drug are crucial for compliance and hence optimal treatment of diseases that require long-term management (eg, osteoporosis). The preference for and ... [more ▼]

Background: Preference for and acceptability of a drug are crucial for compliance and hence optimal treatment of diseases that require long-term management (eg, osteoporosis). The preference for and acceptability of a chewable tablet containing calcium and vitamin D-3 and a dose-comparable effervescent powder were assessed in a Phase 4, randomized, open-label, crossover trial in 5 European countries (Sweden, Finland, Belgium, the Netherlands, and Greece). Objective: The aim of the present analysis was to compare the preference for and acceptability, including tolerability, of these 2 formulations based on the Belgian results of the previously mentioned study. Methods: Patients were recruited from 3 osteoporosis units and university hospitals in Brussels, Liege, and Ghent, Belgium. Adult patients at risk for calcium and vitamin D deficiencies were enrolled. The study drugs included 2 formulations of a dietary supplement containing a combination of calcium plus vitamin D-3: chewable tablets (calcium carbonate, 1250 mg; vitamin D-3, 400 IU) (A) and effervescent powder (calcium carbonate, 1250 mg; vitamin D-3, 440 IU) (B). Patients were randomly assigned to receive 1 of 2 treatment sequences: AB or BA. Both formulations were given PO BID for 14 days, with a switch to the alternate formulation occurring on day 15 of the study. Preference and acceptability were assessed using 2 questionnaires: one assessed 5 variables of acceptability using 11-point scales, and the other assessed preference using yes/no questions. Compliance and tolerability were recorded throughout the study, with unused dose counts and recording of adverse events (AEs), respectively. Results: The study comprised 200 patients, 199 of whom received at least 1 dose of study medication and were included in the intent-to-treat analysis (174 women, 25 men; mean age, 66 years [range, 30-87 years]). Preference data were available in 178 patients, 129 of whom (72.5%) preferred the chewable tablet compared with 34 (19.1%) who preferred the effervescent powder and 15 (8.4%) who had no preference (both, P < 0.001 vs tablet). The preference for the tablet was based on consistently and significantly higher mean scores on all 5 variables of acceptability (all, P < 0.001). The most common AEs were gastrointestinal (tablet, 27/192 patients [14.1%]; powder, 31/190 patients [16.3%]). Eighteen patients (9.0%) discontinued the trial due to >= 1 AE (12 receiving the tablet and 6 receiving the powder). Conclusions: In this study of preference for and acceptability of 2 formulations (chewable tablet and effervescent powder) of a dietary supplement containing a combination of calcium plus vitamin D-3 in Belgian adults at risk for calcium and vitamin D deficiencies, the chewable tablet was preferred by a significant majority. Based on 5 variables, the tablet was found to be significantly more acceptable than the powder. Tolerability was similar between the 2 formulations. Copyright (c) 2005 Excerpta Medica, Inc. [less ▲]

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See detailPreference for rich, random tactile stimulation in woodlice (Porcellio scaber)
Anselme, Patrick ULg

in Learning & Motivation (2013), 44(4), 326-336

All living beings exhibit preferences for a variety of biologically significant stimuli. Interestingly, stimuli without biological significance, such as saccharine, are also able to induce preferences in ... [more ▼]

All living beings exhibit preferences for a variety of biologically significant stimuli. Interestingly, stimuli without biological significance, such as saccharine, are also able to induce preferences in vertebrates. Can invertebrates show preferences for biologically neutral cues as well – i.e. independently of any conditioning process? Experiment 1 aimed to determine the preference of woodlice (Porcellio scaber Latreille 1804) exposed to floor textures that differed in tactile cues, how they expressed their tactile preference, and whether they were able to inhibit that preference when a shelter of variable quality was available on the non-preferred floor texture. Experiment 2 provided additional information relative to the strength of woodlice’s tactile preference as well as the way of measuring it. Experiment 3 complemented the previous one in attempting to determine woodlice’s preference for regular versus random tactile cues. Taken together, the results suggest that (i) woodlice are able to process sensory information relative to biologically non-significant stimuli and (ii) that, because the motivation induced can interact with sheltering as a survival-related behavior, the processing of both types of motivation might depend on the same brain systems. [less ▲]

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See detailLes préférences alimentaires : comment préserver son équilibre alimentaire et sa qualité de vie? De l'enfant à l'adulte.
Etienne, Anne-Marie ULg

Scientific conference (2014, April 24)

Dans notre société actuelle, nous observons un engouement pour les émissions culinaires : Master Chef, Top Chef, un dîner presque parfait, … En même temps, plusieurs études montrent que de nombreuses ... [more ▼]

Dans notre société actuelle, nous observons un engouement pour les émissions culinaires : Master Chef, Top Chef, un dîner presque parfait, … En même temps, plusieurs études montrent que de nombreuses personnes ne mangent pas selon les recommandations actuelles. D’où un intérêt grandissant pour la compréhension des mécanismes liés aux préférences alimentaires : Que se passe-t-il quand un enfant a le choix entre plusieurs aliments ? Comment ces préférences alimentaires évoluent-elles à l’âge adulte ? Les mécanismes d’apprentissage social fournissent des réponses quant aux choix concernant la nourriture ; ceci comprend en particulier les parents et les médias qui véhiculent de l’information ou présentent de nouveaux modèles. Partant de là, nous discuterons de comment concilier nos préférences alimentaires avec un équilibre alimentaire, sans basculer dans une restriction alimentaire qui elle, risque d’affecter notre qualité de vie. [less ▲]

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See detailPreferences of patients for osteoporosis drug treatment: a cross-european discrete choice experiment
Hiligsmann, Mickaël ULg; Dellaert, BG; Dirksen, CD et al

in Osteoporosis International (2014), 25(2), 227-228

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See detailPréférences résidentielles et production du logement en banlieue périurbaine
Halleux, Jean-Marie ULg; Strée, Julie

Conference (2014, May 27)

La communication traitera des relations entre les mécanismes de la production immobilière et les préférences résidentielles. Elle visera à vérifier l’hypothèse selon laquelle nous assistons à l’émergence ... [more ▼]

La communication traitera des relations entre les mécanismes de la production immobilière et les préférences résidentielles. Elle visera à vérifier l’hypothèse selon laquelle nous assistons à l’émergence et au renforcement de préférences susceptibles de remettre en cause le modèle périurbain du pavillon périphérique. Notre propos s’inscrit donc dans la réflexion sur le futur des zones périurbaines (Grant et al., 2013). Notre recherche a consisté dans la réalisation et l’analyse d’une enquête menée au sein de la zone métropolitaine de Bruxelles, auprès de ménages habitant un logement neuf (Strée et Halleux, 2013). Les ménages ont été interrogés sur leurs préférences résidentielles ainsi que sur leurs propensions à payer pour différents types de produits immobiliers. Son échantillon est constitué de 120 ménages ayant été interrogés en face à face à leur domicile. L’échantillon a été stratifié en fonction de deux critères : la localisation au sein de la zone métropolitaine et le type de logements. Concernant la localisation, des enquêtes ont été menées à Wavre (commune située à une demi-heure voiture du centre de Bruxelles) et en plus lointaine périphérie, à Perwez et Chastre (communes situées à une heure voiture du centre de Bruxelles). Concernant les types de logement, la stratification s’est opérée de la manière suivante : 40 % des ménages résident dans une habitation quatre façades, 30 % dans une habitation mitoyenne et 30 % dans un appartement. Un premier résultat de notre enquête est de confirmer la prégnance du modèle de la maison unifamiliale isolée (quatre façades), qui représente le logement idéal pour 69 % de l’échantillon (y compris par 70 % des ménages résidant dans une habitation mitoyenne et par 57 % des ménages résidant en appartement). Pour autant, en comparaison d’enquêtes précédentes, les propensions à payer témoignent d’une bonne acceptation des produits immobiliers alternatifs. Sur base d’enquêtes précédentes réalisées dans le périurbain de Liège, nous nous attendions à une moins-value de 15 à 20 % entre le produit de la maison isolée et le produit de la maison jointive. Or, la différence observée est ici inférieure à 10 %. Cette situation est à relier à la problématique de la disponibilité en zones constructibles (Halleux et Strée, 2012). En effet, le bassin d’emploi bruxellois se caractérisant par une faible disponibilité foncière, cela conduit à banaliser la promotion commerciale de logements jointifs. Au sein de ces territoires, les ménages auraient donc bien intégré ce type de développements. Au sein d’autres territoires aux marchés fonciers moins tendus, nous aurions probablement observé des consentements à payer plus élevés pour la maison isolée, consentements qui auraient alors pu être interprétés comme un signe de stigmatisation vis-à-vis du logement jointif. Au final, nous mettons en avant un effet systémique où, par son impact sur la multiplication des projets de maisons mitoyennes, la rareté de l’offre foncière conduit à renforcer l’acceptation de la demande pour ce type de produits immobiliers. Parmi les autres résultats de notre enquête, soulignons la forte valorisation de la maison de 100 m2 qui, en comparaison d’appartements de la même superficie, est valorisée à un niveau moyen 13 % supérieur. Il existe donc un potentiel commercial important pour la petite maison. Cela représente à la fois une menace pour la consommation d’espace et une opportunité pour la densification douce. Il ressort également de nos enquêtes qu’une localisation intra-urbaine (dans un quartier « ancien ») est fortement dévalorisée. Pour autant, un signal encourageant tient ici à la relation entre l’âge du répondant et cette dévalorisation : les effectifs de moins de 40 ans dévalorisent moins les quartiers densément bâti que les populations plus âgées. Il s’agit là d’un résultat qui s’inscrit dans l’hypothèse de Grant et al. (2013) quant au renforcement de la demande pour des localisations plus urbaines et plus centrales. [less ▲]

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See detailPREFERENTIAL DEACTIVATION OF THE S(3) STATE OF THE WATER-OXIDIZING COMPLEX, FAVORED BY PLASTOQUINONE REDUCTION IN BARLEY CHLOROPLASTS
Franck, Fabrice ULg; SCHMID, G. H.

in Zeitschrift Fur Naturforschung C-A Journal Of Biosciences (1993), 48(7-8), 603-608

The flash patterns of oxygen evolution after pre-illumination by continuous light or by a flash sequence were compared in etiochloroplasts and mature chloroplasts of barley. In both types of plastids sub ... [more ▼]

The flash patterns of oxygen evolution after pre-illumination by continuous light or by a flash sequence were compared in etiochloroplasts and mature chloroplasts of barley. In both types of plastids sub-saturating continuous illumination of some seconds strongly affected the S3 but not the S2 state of the oxygen-evolving complex. This result is ascribed to efficient charge recombination of S3 with the acceptor side of photosystem II, favoured by partial reduction of the plastoquinone pool. The increase of S3 observed in the presence of dichlorophenolindophenol in etiochloroplasts confirms this interpretation. These observations strengthen the recent hypothesis of a conformational change during the transition from S2 to S3 recently proposed to interpret the different susceptibility of these two states to hydroxylamine and hydrazine (F. Frank and G. H. Schmid, Biochim. Biophys. Acta 977, 215-218 (1989); J. Messinger, U. Wacker, and G. Renger, Biochemistry 30,7852-7862 (1991)). [less ▲]

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See detailA PREFERENTIAL FOLDED CONFORMATION OF SOME BIS-(8-ISOPROPYL-ISOQUINOLINIUM) DERIVATIVES EXPLAINS STEREOSELECTIVE REDUCTION BY SODIUM BOROHYDRIDE
Dilly, Sébastien ULg; Badarau, Eduard; Dufour, Fabien et al

Poster (2014, June 05)

Small conductance Ca2+-activated K+ (SK) channels play a role in modulating the firing rate and the firing pattern of neurons [Waroux, Eur J Neurosci, 2005, 22, 3111]. A blockade of these targets could be ... [more ▼]

Small conductance Ca2+-activated K+ (SK) channels play a role in modulating the firing rate and the firing pattern of neurons [Waroux, Eur J Neurosci, 2005, 22, 3111]. A blockade of these targets could be useful for the treatment of cognitive dysfunction, neuronal hyperexcitability or dopamine related disorders [Liégeois, Curr Med Chem, 2003, 10, 625]. At the peripheral level, the inhibition of these channels was demonstrated to prevent and terminate atrial fibrillation [Diness, Circ Arrhythm Electrophysiol, 2010, 3, 380]. Moreover, SK channels might represent potential targets for a new class of anticancer agents due to their involvement in breast cancer cell migration [Potier, Mol Cancer Ther, 2006, 5, 2946]. So far, available blockers are not suitable CNS pharmacological tools being either peptides or small molecules with permanent positive charges [Liégeois, Curr Med Chem, 2003, 10, 625; Graulich, J Med Chem, 2007, 50, 5070; Badarau, Bioorg Med Chem Lett, 2011, 21, 6756]. Therefore, symmetrical bis-isoquinolinium compounds have subsequently been transformed to 1,2,3,4-tetrahydroisoquinoline analogues by using sodium borohydride leading to a diastereoisomeric mixture (figure 1) in order to obtain potential CNS-penetrating agents [Graulich, Bioorg Med Chem Lett, 2008, 18, 3440; Neny, Proceedings of the 12th International Conference on In Vivo Methods, Vancouver, Canada, 2008, 267; Koulchitsky, Acta Physiologica, 2009, 195, 670]. Resolution of these mixtures and characterization of the corresponding stereoisomers [Wouters, Eur J Med Chem, 2010, 45, 3240] are necessary before further biological evaluation. In a series of 8- isopropyl analogues, chiral resolution failed for the analogues with propyl and m-xylyl linkers since two and one peaks, respectively, were detected [Nistor, J Pharm Biomed Anal, 2013, 74, 273]. Could these results be explained by an ineffective resolution or would another phenomenon be involved? Further analysis using chiral chromatography, mass spectroscopy and circular dichroism of a sample of the propyl analogue revealed that it is a racemic mixture. X-ray cristallography and conformational analysis indicated a folded conformation of the propyl and m-xylyl analogues (figure 2) responsible for a stereoselective attack of the borohydride reagent during the reduction step. Additional 1H-NMR investigations support structural features detected by theoretical analysis. [less ▲]

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See detailPreferential role of calcium in the regulation of prolactin gene transcription by thyrotropin-releasing hormone in GH3 pituitary cells
Laverriere, Jean-Noël; Tixier-Vidal, Andrée; Buisson, Nicole et al

in Endocrinology (1988), 122(1), 333-40

TRH induces two separate events in pituitary PRL cells. It increases the release of stored PRL and enhances the rate of PRL gene transcription, which results in an increased steady state concentration of ... [more ▼]

TRH induces two separate events in pituitary PRL cells. It increases the release of stored PRL and enhances the rate of PRL gene transcription, which results in an increased steady state concentration of PRL messenger RNA (mRNA) and a concomitant augmentation of PRL production. The mechanisms underlying the release process involve the activation of phosphatidylinositol turnover which generates inositol 1,4,5-trisphosphate and 1,2-diacylglycerol. In order to determine whether these intracellular messengers also mediate the stimulation of PRL gene expression by TRH, we have correlated the level of receptor occupancy with the rate of gene transcription and investigated the action of drugs which increase cytosolic calcium or activate protein kinase C. We have determined that sustained stimulation of transcription requires the persistent occupancy of a limited number of TRH receptor sites and that the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), calcium ionophores (A23187, ionomycin), and the calcium channel agonist BAY K 8644 enhance PRL gene transcription. However, TPA is less potent and ionomycin requires a low concentration of TPA to fully mimic TRH action, whereas BAY K 8644 alone displays the same potency as TRH. The effects of BAY K 8644 and TRH are not additive and thus suggest that the influx of calcium plays a predominant role in the regulation of PRL gene transcription by TRH. [less ▲]

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See detailPreferential transmission of Gossypium sturtanium chromosome fragments in the progeny of [(G. hirsutum x G. raimondii)2 x G. sturtanium] trispecies hybrid
Benbouza, H.; Diouf F.B.H.; Ndir, K. et al

in Proceedings of World cotton research conference IV (2008)

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See detailThe prefix be-/bi- as a marker of verbs of deception in late Old and early Middle English
Petré, Peter ULg

in BELL. Belgian Journal of English Language and Literatures (2006), 4

This article brings to light a productive inseparable prefix construction marked by be-/biin late Old English and early Middle English, which forms complex verbs of DECEPTION, such as betray, beguile ... [more ▼]

This article brings to light a productive inseparable prefix construction marked by be-/biin late Old English and early Middle English, which forms complex verbs of DECEPTION, such as betray, beguile, bewray. The expression of such a specific psychological sense through a verbal prefix is highly exceptional for Germanic languages, and a complex four stage development has to be assumed, starting from other concrete constructions marked by be-/bi- such as the PRIVATIVE construction (cf. bereave, behead). By emphasizing the relevance of the notion of construction in this development, the article also contributes to the insights in the relationship between constructions and language change. [less ▲]

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See detailPregnancies after vitrification of human day 5 embryos
Vanderzwalmen, Pierre ULg; Delval, Alain; Chatziparasidou, A. et al

in Human Reproduction (1997), 12(suppl), 98

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See detailPregnancies, calves and calf viability after transfer of in vitro produced bovine embryos
Schmidt, T.; Greve, T.; Avery, B. et al

in Theriogenology (1996), 46(3), 527-539

Pregnancy, parturition and calf survival following the transfer of embryos produced in vitro were monitored. A total of 44 blastocysts was transferred in pairs to 1 uterine horn ipsilateral to the corpus ... [more ▼]

Pregnancy, parturition and calf survival following the transfer of embryos produced in vitro were monitored. A total of 44 blastocysts was transferred in pairs to 1 uterine horn ipsilateral to the corpus luteum (CL) of 22 synchronized heifers. At Day 42 of development 14 recipients (64%) were pregnant; the calving rate was also 64%. The twinning rate was 9/14 at Day 42 and 7/14 at birth, for an overall fetal mortality rate of 9%. The average gestation length was 281 and 275 d for single and twin pregnancies, respectively. Blood samples from recipients were collected for determination of bovine pregnancy associated glycoprotein (bPAG) from 2 wk after transfer and throughout the pregnancy. During the first trimester of pregnancy, the bPAG concentration was significantly higher in twin than in single bearing heifers, and the perinatal increase in bPAG was correlated positively with the total weight of the fetus(es). The percentage of male calves was 43%. The birth weight of twin individuals was 25 ± 1 kg, which was 78% of the birthweight of the singletons (32 ± 2 kg). One singleton calf was oversized, weighing 58 kg (80% more than the median weight of the other singletons). Stillbirths occurred in 21% of the twins, butin none of the singletons. Calf mortality during the first 14 d was higher for twins (4/11) than for singletons (1/7) due to infections and cerebellar hypoplasia. Karyotyping the calves detected no cytogenetically recognizable abnormalities. All calves were negative for BVD virus and IBR antibodies. The results of this study showed that although the incidence of fetal loss was low, there was an unacceptable high perinatal mortality of the calves. Thus it is likely that the blood supply through the placenta of animals pregnant with twins was impaired or it is possible that these fetuses and calves had increased stress susceptibility caused by the in vitro conditions. Furthermore, the birth of 1 oversized calf, 2 calves with cerebellar hypoplasia and 5 calves succumbing to infections seems to indicate that a proportion of in vitro produced calves may suffer from factors inherent in the in vitro production system [less ▲]

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See detailPregnancy and inflammatory bowel disease
Louis, Edouard ULg; Belaiche, Jacques ULg

in Acta Gastro-Enterologica Belgica (2002), 65(4, Oct-Dec), 230-232

Overall, around 25% of women with inflammatory bowel disease will conceive during their disease. Most of the women with inflammatory bowel disease will have a normal pregnancy and healthy children ... [more ▼]

Overall, around 25% of women with inflammatory bowel disease will conceive during their disease. Most of the women with inflammatory bowel disease will have a normal pregnancy and healthy children. However, specific problems may arise related to these pregnancies. This paper reviews what is known on fertility, risk of disease transmission, effect of the disease on the pregnancy and the reverse, delivery, medical follow up and treatment as well as breastfeeding in the setting of inflammatory bowel disease. [less ▲]

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See detailPregnancy and the immune system: between tolerance and rejection
Thellin, Olivier ULg; Heinen, Ernst ULg

in Toxicology (2003), 185(3), 179-184

Interactions between the conceptus and the mother are bi-directional: the feto-placental tissues need nutrition and a suitable environment in homeostatic condition whereas the mother influenced by the ... [more ▼]

Interactions between the conceptus and the mother are bi-directional: the feto-placental tissues need nutrition and a suitable environment in homeostatic condition whereas the mother influenced by the placental factors adapts her metabolism and immune system. Many different mechanisms acting locally or at distance ensure tolerance of the semi-allogeneic graft by the maternal natural and adaptive immune defences. In front of this tolerance, mechanisms exist ensuring rejection of the conceptus by the mother (spontaneous abortion) through rupture of one or more tolerance mechanisms, notably in stress situations endangering the mother. Thus outcome of a pregnancy is dependent on efficiently working tolerance mechanisms, and rupture of such mechanisms can lead to rejection. The balance of influence leading either to tolerance or rejection is under control of internal (maternal and fetal) and external (environmental) factors. Rejection, if triggered, mainly occurs through immune-induced inflammation, tissue degradation and coagulation. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved. [less ▲]

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See detailPregnancy associated glycoprotein-1,-6,-7, and-17 are major products of bovine binucleate trophoblast giant cells at midpregnancy
Klisch, K.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in Molecular Reproduction and Development (2005), 71(4), 453-460

Pregnancy associated glycoproteins (PAGs) are extensively glycosylated secretory proteins of ruminant trophoblast cells. In cattle placenta several PAG cDNAs are expressed, but the variety of ... [more ▼]

Pregnancy associated glycoproteins (PAGs) are extensively glycosylated secretory proteins of ruminant trophoblast cells. In cattle placenta several PAG cDNAs are expressed, but the variety of correspondent proteins and their degree of glycosylation are not well characterized. Thus, we purified PAGs by using a protocol which included a lectin (Vicia villosa agglutinin) affinity chromatography. Due to their specific glycosylation pattern, PAGs derived from binucleate trophoblast giant cells were highly enriched by this protocol. PAGs were purified from cotyledons of 2 day 100 placentas and from a single placenta at day 155 and 180. In all samples three major bands (75; 66; 56 kDa) were detected by one-dimensional SDS-PAGE. Mass-spectrometric analysis identified the 75 kDa band as a mixture of PAG-7 and PAG-6, the 66 kDa band as PAG-1 and the 56 kDa band as PAG-17. N-terminal sequencing of the day 100 sample confirmed the mass spectrometric identifications. Enzymatic release of N-glycans with peptide-N-glycanase-F from PAGs reduced the molecular weight to approximately 37 kDa which corresponds to the theoretical molecular mass of PAGs. Limited peptide-N-glycanase-F treatment revealed that all four N-glycosylation sites are quantitatively occupied in PAG-1. Compared to PAG-1 the number of potential N-glycosylation sites is lower in PAG-17 (three sites) and higher in PAG-6 and -7 (five and six sites, respectively). This suggests that the number of attached N-glycans is the main determinant of molecular mass of bovine PAGs. The degree of glycosylation may be a major factor regulating the plasma half life of PAGs. [less ▲]

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See detailPregnancy associated glycoproteins (PAG) in postpartum cows, ewes, goats and their offspring
Haugejorden, G.; Waage, S.; Dahl, E. et al

in Theriogenology (2006), 66(8), 1976-1984

Determination of plasma concentrations of pregnancy associated glycoproteins (PAG) has been used for early pregnancy diagnosis in cows. However, this is complicated by the presence of PAG in plasma for an ... [more ▼]

Determination of plasma concentrations of pregnancy associated glycoproteins (PAG) has been used for early pregnancy diagnosis in cows. However, this is complicated by the presence of PAG in plasma for an extended period postpartum. The main objective of the present study was to investigate the postpartum elimination rates of pregnancy associated glycoproteins (PAG) in sheep, goats and cows in order to gain background information applicable to the use of PAG for pregnancy diagnosis in domestic ruminants. A second objective was to investigate whether PAG are transferred to the foetus and newborn, by measuring plasma PAG concentrations in calves, lambs and goat kids before and after colostrum feeding. PAG in the blood at parturition were eliminated by a first order process in the cows and ewes, while a two-step log-linear decline occurred in the goats. Estimated postpartum half-life of plasma PAG in the cows and ewes was 9 and 4.5 days, respectively. In the goats, half-lives were 3.6 and 7.5 days in the initial fast and terminal slow phase. Basal levels were reached 80-90 days postpartum in cows. Plasma PAG concentration can be used for pregnancy diagnosis from day 28 after AI, provided that the time interval from calving to AI is > 60 days. Using a heterologous antibody RIA, we found 4 ng/mL to be the appropriate cut-off. Due to the presence of PAG residues from the previous gestation, the interval from AI to pregnancy diagnosis should increase by approximately 0.5 days beyond 28 days for each day of AI closer to calving than 60. Measurements in newborn ruminants suggested that PAG enter the foetal blood in utero and that colostral PAG are transferred to the newborn. Following the peak plasma concentration observed 1 day after birth in most of the animals, PAG were rapidly eliminated in a log-linear fashion. [less ▲]

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See detailPregnancy Associated Glycoproteins in Ruminants: Inactive Members of the Aspartic Proteinase Family
Beckers, Jean-François ULg; Drion, Pierre ULg; Garbayo, J. M. et al

in Acta Veterinaria Hungarica (1999), 47(4), 461-9

The Pregnancy Associated Glycoproteins (PAGs) presented in this paper are largely expressed in the ruminant placenta. These proteins are classified as probably inactive members of the aspartic proteinase ... [more ▼]

The Pregnancy Associated Glycoproteins (PAGs) presented in this paper are largely expressed in the ruminant placenta. These proteins are classified as probably inactive members of the aspartic proteinase family. Pepsinogen, renin, cathepsin E [less ▲]

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