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See detailPlasma Estradiol Concentrations and Pharmacokinetics Following Transdermal Application of Menorest 50 or Systen (Evorel) 50
Reginster, Jean-Yves ULg; Albert, Adelin ULg; Deroisy, Rita ULg et al

in Maturitas (1997), 27(2), 179-86

OBJECTIVES: In order to compare the pharmacokinetics of two transdermal estrogen replacement therapy (ERT) systems designed to release 50 micrograms 17 beta-estradiol/day, two studies were performed in ... [more ▼]

OBJECTIVES: In order to compare the pharmacokinetics of two transdermal estrogen replacement therapy (ERT) systems designed to release 50 micrograms 17 beta-estradiol/day, two studies were performed in healthy postmenopausal volunteers. METHODS: Both studies had a cross-over design and incorporated a 1-week wash-out period between treatments. In the first study, Menorest 50 and Systen 50 (Evorel 50) were compared over four days of application in 30 women. In the second, 13 women wore each of the two systems for a total of 12 days each (three patches each for 4 days), and comparison was made during the third patch period (steady state, between days 8 and 12). Plasma 17 beta-estradiol levels were assayed using specific direct radioimmunoassays, and pharmacokinetic parameters were calculated by standard methods. All the samples of the first study were re-analysed using a different radioimmunoassay and the results of both assays were compared. RESULTS: In both studies, plasma 17 beta-estradiol levels rose at a comparable rate and reached similar peak levels with each of the two formulations. Levels then remained relatively constant throughout both evaluation periods with Menorest 50, but began to decline after 12 hours in the first study and after 30 h under steady state conditions in the second study with Systen 50. The difference between the two products was statistically significant in both studies. Analysis of pharmacokinetic parameters confirmed the greater bioavailability of Menorest 50. In addition, 17 beta-estradiol levels remained within the suggested therapeutic ranges for relief of acute symptoms and protection against osteoporosis for longer periods of time with Menorest 50 than with Systen 50. CONCLUSION: Since the acute efficacy, long-term protective effects, side effects and risks associated with ERT may depend on critical threshold plasma levels, much attention should be paid to the pharmacokinetic profiles of different formulations. The comparison of these two different radioimmunoassays demonstrates the comparability of their results. [less ▲]

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See detailPlasma evolution of testosterone, insulin-like growth factor-I and IGF-I binding protein capacities around puberty in cattle
Massart, Serge; Renaville, Robert ULg; Sneyers, Myriam et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1991), 99

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See detailPlasma Histamine and Bronchial Reactivity in Allergic Asthma
Corhay, Jean-Louis ULg; Bury, Thierry ULg; Louis, Renaud ULg et al

in Allergy (1993), 48(7), 547-9

Histamine is an important mediator of allergic inflammation and bronchial hyperresponsiveness (BHR), a hallmark of asthma. Studies on the relationship between plasma histamine and BHR in allergic ... [more ▼]

Histamine is an important mediator of allergic inflammation and bronchial hyperresponsiveness (BHR), a hallmark of asthma. Studies on the relationship between plasma histamine and BHR in allergic asthmatic patients have yielded controversial results. We therefore measured plasma histamine and bronchial reactivity in 30 nonsmoker volunteers taking no medication. Eleven were normal subjects; 19 were stable, mildly allergic asthmatic patients. Venous blood was taken to measure blood cells and basal plasma histamine by radioimmunoassay. After blood sampling, all subjects underwent a measurement of PC20M (concentration of methacholine causing a 20% fall in FEV1). Mean plasma histamine levels were 0.21 +/- 0.1 ng/ml and 0.44 +/- 0.3 ng/ml in normal and asthmatic subjects, respectively (P < 0.05). We found a significant increase of blood eosinophils and basophils in asthmatic patients, and a positive correlation between plasma histamine and circulating basophils. PC20M was greater than 16 mg in normal volunteers, and mean PC20M was 2.1 +/- 2 mg/ml in asthmatic patients. PC20M did not correlate with plasma histamine levels, but it did so negatively with blood eosinophils. The increased plasma histamine concentration in mildly atopic asthmatic patients might be a consequence of the high basophil releasability of atopics and the higher basophil counts in allergic asthma. Plasma histamine is thus unlikely to be a determinant of BHR in asthma. [less ▲]

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See detailPlasma Hormones and Metabolites in Cattle in Relation to Breed (Belgian Blue Vs Holstein) and Conformation (Double-Muscled Vs Dual-Purpose Type)
Clinquart, Antoine ULg; Van Eenaeme, Christian ULg; Mayombo, A. P. et al

in Veterinary Research Communications (1995), 19(3), 185-94

Four Belgian Blue double-muscled type (BBDM) bulls, four Belgian Blue dual-purpose type (BBDP) bulls and four Holstein bulls were used in a fattening trial in order to relate differences in the extent of ... [more ▼]

Four Belgian Blue double-muscled type (BBDM) bulls, four Belgian Blue dual-purpose type (BBDP) bulls and four Holstein bulls were used in a fattening trial in order to relate differences in the extent of muscle development and adiposity to differences in digestibility, endocrine status, protein and lipid metabolism. The larger muscle development in BBDM animals was associated with a trend to higher nitrogen retention, higher food conversion efficiency (p < 0.05) and lower apparent digestibility (p < 0.05). No difference was found between the groups for plasma glucose concentration. Higher creatinine, lower alpha-amino nitrogen, lower triglycerides and higher non-esterified fatty acid plasma levels were observed in BBDM as compared to Holstein bulls (p < 0.05), the BBDP group being intermediate. A trend to a higher cholesterol plasma level was found in BBDM animals. There was no difference between the three groups in plasma fatty acid composition, except for the C14:0 content. Some of the differences in plasma metabolites were related to carcass composition and endocrine regulation, a decrease in muscle development and an increase in adiposity being associated with lower growth hormone production (p < 0.05) and higher insulin (p < 0.05) and IGF secretions. The insulin/growth hormone ratio at the end of the fattening period was 0.0011, 0.0018 and 0.0069 in BBDM, BBDP and Holstein bulls, respectively, and was directly associated with fat deposition. [less ▲]

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See detailPlasma IGF-I levels as predictive criteria of growth performances in cattle
Renaville, Robert ULg; Sneyers, Myriam; Massart, Serge et al

Poster (1994)

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See detailPlasma IGF-I levels as predictive criteria of growth performances in cattle
Renaville, Robert ULg; Sneyers, Myriam; Massart, Serge et al

in Growth Regulation (1994), 4(suppl 1), 115

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See detailPlasma influence on cellular calcium (Ca IC) regulation in chronic renal failure (CRF) and essential hypertension
Krzesinski, Jean-Marie ULg; Pequeux, M. L.; Rorive, Georges ULg

in Abstract book of XII International Congress of Nephrology (1993)

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See detailPlasma insulin level in double-muscled and conventional bulls during the first year of life
Michaux, C.; Beckers, Jean-François ULg; de Fonseca, M. et al

in Zeitschrift für Tierzüchtung und Züchtungsbiologie (1981), 98

Concentration of plasma insulin has been determined by radioimmunoassay in conventional and double-muscled bulls at 80 (I1), 131 (I2), 227 (I3) and 370 I(4) d of age. Double-musceld have lower plasma ... [more ▼]

Concentration of plasma insulin has been determined by radioimmunoassay in conventional and double-muscled bulls at 80 (I1), 131 (I2), 227 (I3) and 370 I(4) d of age. Double-musceld have lower plasma levels of insulin at all ages studied, which is consistent with the reduced fatty tissue of double-muscling. The correlations between insulin level and growth traits, estimated separately fo reach type of bulls, are low and most often positive and significant in double-muscled. Insulin level tends to be negatively correlated with the lean percent from the 7th rib cut. The correlation between the successive insulin levels are generally positive. The correlations between the concentration of insulin and of GH are low and negative. [less ▲]

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See detailPlasma leptin levels, insulin secretion, clearance and action on glucose metabolism in anorexia nervosa.
Letiexhe, Michel ULg; Scheen, André ULg; Lefebvre, Pierre ULg

in Eating and Weight Disorders [=EWD] (1997), 2(2), 79-86

From a metabolic point of view, anorexia nervosa may be viewed as a mirror image of obesity. We compared insulin secretion, clearance and action on glucose metabolism during an intravenous glucose ... [more ▼]

From a metabolic point of view, anorexia nervosa may be viewed as a mirror image of obesity. We compared insulin secretion, clearance and action on glucose metabolism during an intravenous glucose tolerance test in nine women with anorexia nervosa and in nine age-matched normal-weight controls. Insulin secretion (ISR) was derived by deconvolution of plasma C-peptide levels, insulin clearance (MCR(I)) was obtained by dividing the area under the curve (AUC(0-180 min)) of ISR by the corresponding AUC of plasma insulin levels, insulin sensitivity (S(I)) and glucose effectiveness index (S(G)) were calculated by Bergman's minimal model. The anorectic women had markedly lower BMI values (13.7+/-0.6 vs 23.2+/-0.8 kg/m2, p<0.0001) and serum basal leptin levels (2.8+/-0.6 vs 8.9+/-1.8 ng/mL, p=0.005) than control women. The anorectic women exhibited clear-cut lower fasting and post-glucose plasma insulin levels but similar corresponding plasma C-peptide concentrations when compared to controls. Consequently, ISR was similar in both groups while MCR(I) was significantly increased in anorexia nervosa (MCR(I): 3320+/-881 vs 822+/-79 mL x min(-1) x m(-2), p<0.02). The index S(I) tended to be higher in anorectic women than in normal-weight subjects, but without reaching the level of statistical significance because of a high between-subject variability (20.2+/-5.7 vs 12.5+/-2.2 10(-5) x min(-1)/pmol x L(-1), NS). The index S(G) was similar in both groups (0.022+/-0.004 vs 0.018+/-0.002 min(-1), NS). In conclusion, low plasma insulin levels observed in women with anorexia nervosa result from high MCR(I) rather than from depressed insulin secretion. Insulin sensitivity is not systematically increased and glucose effectiveness is unchanged in anorectic women when compared to normal-weight controls. [less ▲]

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See detailPlasma luteinizing hormone and progesterone concentrations in Azawak zebu cows submitted to different estrus synchronization protocols.
Zongo, M.; Pitala, W.; Sawadogo, L. et al

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (2008), 61(3-4), 221-227

The percentage of estrus induction and plasma LH concentrations were examined in 15 Azawak zebu cows submitted to two different estrus synchronization protocols. In the first treatment (T1, n = 9), the ... [more ▼]

The percentage of estrus induction and plasma LH concentrations were examined in 15 Azawak zebu cows submitted to two different estrus synchronization protocols. In the first treatment (T1, n = 9), the cows received a norgestomet ear-implant for 10 days associated with estradiol valerate, prostaglandin F2alpha (PGF2alpha) and pregnant mare serum gonadotropin (PMSG) injections. Treatment 2 (T2, n = 6) consisted in two PGF2alpha injections 11 days apart, followed by the administration of PMSG two days after the second prostaglandin injection. Blood samples were collected every three hours during five days after implant removal (T1) or PMSG injection (T2) for LH measurements. The proportion of induced estrus was higher in T2, but the difference was not significant. In general, the elapsed time until the estrus onset tended to be shorter in norgestomet-treated animals (35.9 ± 3.9 h) than in prostaglandin-treated ones (49.5 ± 5.8 h). Seven out of nine norgestomet-treated cows, and only two out of the six treated with PGF2alpha, presented a peak of LH. The mean interval from the end of treatments to LH peak tended to be longer in PGF2-treated females than in norgestomet-treated ones. One female presenting abnormally high LH concentrations after implant removal did not show a peak of LH during the observation period. [less ▲]

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See detailPlasma membrane localization of StREM1.3 Remorin is mediated by conformational changes in a novel C-terminal anchor and required for the restriction of PVX movement.
Perraki, Artemis; Cacas, Jean-Luc; Crowet, Jean-Marc ULg et al

in Plant Physiology (2012), 160(1),

The formation of plasma membrane (PM) micro-domains plays a crucial role in the regulation of membrane signalling and trafficking. Remorins are a plant-specific family of proteins organized in six ... [more ▼]

The formation of plasma membrane (PM) micro-domains plays a crucial role in the regulation of membrane signalling and trafficking. Remorins are a plant-specific family of proteins organized in six phylogenetic groups, and Remorins of the group 1 are among the few plant proteins known to specifically associate with membrane rafts. As such, they are valuable to understand the molecular bases for PM lateral organization in plants. However, little is known about the structural determinants underlying group 1 Remorins specific association with membrane rafts. We used a structure-function approach to identify a short C-terminal anchor (RemCA) indispensable and sufficient for tight direct binding of Solanum tuberosum REMORIN 1.3 (StREM1.3) to the PM. RemCA switches from unordered to an alpha-helical structure in a non-polar environment. Protein structure modelling indicates that RemCA folds into a tight hairpin of amphipathic helices. Consistently, mutations reducing RemCA amphipathy abolished StREM1.3 PM localization. Furthermore, RemCA directly binds to biological membranes in vitro, shows higher affinity for Detergent-Insoluble Membranes (DIM) lipids, and targets YFP to DIMs in vivo. Mutations in RemCA resulting in cytoplasmic StREM1.3 localization abolish StREM1.3 function in restricting potato virus X movement. The mechanisms described here provide new insights on the control and function of lateral segregation of plant PM. [less ▲]

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See detailPlasma Membrane-Dependent Activation of Gelatinase a in Human Vascular Endothelial Cells
Lewalle, J. M.; Munaut, Carine ULg; Pichot, B. et al

in Journal of Cellular Physiology (1995), 165(3), 475-83

The initiation of the angiogenic process requires a locally confined and time-limited proteolysis of the basement membrane (BM) components at the site of new vessel sprout. Gelatinase A, a member of the ... [more ▼]

The initiation of the angiogenic process requires a locally confined and time-limited proteolysis of the basement membrane (BM) components at the site of new vessel sprout. Gelatinase A, a member of the matrix metalloproteinase family, degrades BM type IV collagen and is involved in the BM breakdown by migrating tumor cells and endothelial cells (EC). Gelatinase A is synthesized as latent proenzyme and must be activated in order to express its proteolytic activity. A plasma membrane-dependent mechanism of activation has been described for several tumor and transformed cells lines. In the present study, we show that latent (72 kD) and mature (62-59 kD) forms of gelatinase A are present in EC membrane fraction from Triton X-114 extract while only latent form is found in the cytosolic fraction. The incubation of EC membrane fraction with exogenous latent gelatinase A resulted in a significant activation giving rise to 62-59 kD mature forms. 12-O-tetradecanoylphorbol-13-acetate (TPA), a strong potentiator of angiogenesis in vitro and in vivo, increases the amount of both latent and activated forms of gelatinase A in EC membrane fraction as well as the ability of this latter fraction to activate exogenous latent gelatinase A. We show that the mRNA transcript coding for the membrane-integrated MMP, the MT-MMP, previously described as a potential gelatinase A activator in invasive tumor cells is also expressed in vascular EC and is regulated through a TPA sensitive process. This enzyme may be responsible for membrane-dependent gelatinase A activation in normal vascular EC and may therefore be a determinant in the control of BM proteolysis during angiogenesis. [less ▲]

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See detailPlasma Motion and Kinematics in Cool and Hot Stars
Güdel, Manuel; Nazé, Yaël ULg

in Space Science Reviews (2010), 157

The environments of both hot and cool stars are the sites of highly dynamic processes involving motion of gas and plasma in winds, flows across shocks, plasma motions in closed magnetic fields, or streams ... [more ▼]

The environments of both hot and cool stars are the sites of highly dynamic processes involving motion of gas and plasma in winds, flows across shocks, plasma motions in closed magnetic fields, or streams along magnetospheric accretion funnels. X-ray spectroscopy has opened new windows toward the study of these processes. Kinematics are evident in line shifts and line broadening, and also more indirectly through the analysis and interpretation of density-sensitive lines. In hot stellar winds, expanding-wind kinematics are directly seen in broadened lines although the broadening has turned out to often be smaller than anticipated, and some lines are so narrow that coronal models have been revived. Although X-ray spectra of cool stars have shown line shifts and broadening due to the kinematics of the entire corona, e.g., in binary systems, intrinsic mass motions are challenging to observe at the presently available resolution. Much indirect evidence for mass motion in magnetic coronae is nevertheless available. And finally, spectral diagnostics has also led to a new picture of X-ray production in accreting pre-main sequence stars where massive accretion flows collide with the photospheric gas, producing shocks in which gas is heated to high temperatures. We summarize evidence for the above mechanisms based on spectroscopic data from XMM-Newton and Chandra. [less ▲]

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See detailPlasma myeloperoxidase and vitamin E levels in head injury: preliminary results related to outcome.
Hans, Pol ULg; Franssen, Colette ULg; Pincemail, Joël ULg et al

in Journal of Neurosurgical Anesthesiology (1992), 4(1), 26-30

This preliminary study was designed to assess a possible role of neutrophil activation and to determine the prognostic value of plasma myeloperoxidase (MPO) and vitamin E (Vit. E) levels in severe head ... [more ▼]

This preliminary study was designed to assess a possible role of neutrophil activation and to determine the prognostic value of plasma myeloperoxidase (MPO) and vitamin E (Vit. E) levels in severe head injury. Plasma MPO and Vit. E levels were measured in nine severely head-injured patients (Glasgow Coma Score </=8) (ages 12-80 years) 6, 12. 18, 24, and 30 h after trauma. Patients were classified into two groups according to outcome after discharge from the ICU: group D (death; n = 5) and group S (survival; n = 4). Plasma MPO levels were increased immediately after trauma and then decreased. The MPO peak observed after 6 h was significantly higher (p < 0.05) in group D (mean +/- SEM: 1,237 +/- 122 ng/ml) than in group S (mean +/- SEM: 543 +/- 148 ng/ml). Plasma Vit. E levels were lower than normal values and decreased over time. They were always significantly lower (p < 0.05) in group D than in group S, except for the first sample. These differences cannot be explained entirely by total plasma lipid (TL) values since no statistical difference in TL concentrations was found between the two groups during the course of study. The ratio of Vit. E to TL. considered as the best index of Vit. E status, was lower in group D than in group S. and the difference reached statistical significance (p < 0.05) 12 h after trauma. In conclusion, in spite of the limited number of patients included in this study, it appears that severe head injury is associated with an increase in MPO and a decrease in Vit. E levels. These biochemical changes are of greater magnitude in group D than in group S; they suggest neutrophil activation and lipoperoxidation processes. Finally, plasma MPO and Vit. E seem to be new discriminant factors of outcome in head-injured patients. [less ▲]

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See detailPlasma Myeloperoxidase Level and Polymorphonuclear Leukocyte Activation in Horses Suffering from Large Intestinal Obstruction Requiring Surgery: Preliminary Results
Grulke, Sigrid ULg; Benbarek, Hama; Caudron, I. et al

in Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Vétérinaire (1999), 63(2), 142-7

Myeloperoxidase (MPO) is a specific enzyme of neutrophil azurophilic granules with a strong oxidative activity. Thanks to a radioimmunoassay of equine myeloperoxidase, the authors have observed a ... [more ▼]

Myeloperoxidase (MPO) is a specific enzyme of neutrophil azurophilic granules with a strong oxidative activity. Thanks to a radioimmunoassay of equine myeloperoxidase, the authors have observed a significantly higher plasma level of MPO in horses operated for strangulation obstruction of the large intestine (n = 6) than in horses suffering from a non-strangulating displacement of the large intestine (n = 9). For the 2 groups, 3 phases were distinguished: reception (P1), intensive care (P2) and terminal phase (P3). The mean peak values of MPO for these phases were 121.6 ng/mL (P1), 168.6 ng/mL (P2), and 107.0 ng/mL (P3) for the non-strangulating group, and 242.6 ng/mL (P1); 426.0 ng/mL (P2), and 379.5 ng/mL (P3) for the strangulation group. The variations of the mean peak values of plasma MPO were significantly different between the 2 groups and between the different phases. A significant increase of the least square means of MPO was observed between P1 and P2. A significant decrease of the least square means of the number of circulating leukocytes was observed between P1 and P3. Polymorphonuclear neutrophil activation could play a major role in the pathogenesis of acute abdominal disease and endotoxic shock. [less ▲]

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See detailPlasma myeloperoxidase level and polymorphonuclear leukocyte activation in horses suffering from large intestinal obstruction requiring surgery: preliminary results.
Grulke, Sigrid ULg; Benbarek, H.; Caudron, I. et al

in Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Vétérinaire (1999), 63(2), 142-7

Myeloperoxidase (MPO) is a specific enzyme of neutrophil azurophilic granules with a strong oxidative activity. Thanks to a radioimmunoassay of equine myeloperoxidase, the authors have observed a ... [more ▼]

Myeloperoxidase (MPO) is a specific enzyme of neutrophil azurophilic granules with a strong oxidative activity. Thanks to a radioimmunoassay of equine myeloperoxidase, the authors have observed a significantly higher plasma level of MPO in horses operated for strangulation obstruction of the large intestine (n = 6) than in horses suffering from a non-strangulating displacement of the large intestine (n = 9). For the 2 groups, 3 phases were distinguished: reception (P1), intensive care (P2) and terminal phase (P3). The mean peak values of MPO for these phases were 121.6 ng/mL (P1), 168.6 ng/mL (P2), and 107.0 ng/mL (P3) for the non-strangulating group, and 242.6 ng/mL (P1); 426.0 ng/mL (P2), and 379.5 ng/mL (P3) for the strangulation group. The variations of the mean peak values of plasma MPO were significantly different between the 2 groups and between the different phases. A significant increase of the least square means of MPO was observed between P1 and P2. A significant decrease of the least square means of the number of circulating leukocytes was observed between P1 and P3. Polymorphonuclear neutrophil activation could play a major role in the pathogenesis of acute abdominal disease and endotoxic shock. [less ▲]

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See detailPlasma Na-K ATPase inhibitor activity and intracellular ions during hemodialysis
Krzesinski, Jean-Marie ULg; Du, F.; Pequeux, M. L. et al

in International Journal of Artificial Organs (1993), 16(1), 23-30

We have investigated the relationship between plasma Na-K ATPase inhibitor activity (EDLS) and intracellular ions in 37 uremic hemodialysed hypertensive patients, and in 20 normotensive non uremic ... [more ▼]

We have investigated the relationship between plasma Na-K ATPase inhibitor activity (EDLS) and intracellular ions in 37 uremic hemodialysed hypertensive patients, and in 20 normotensive non uremic controls (NC). As compared with the NC population, significantly enhanced values for erythrocyte (RBC) Na, Ca, platelet cytosolic Ca and EDLS were observed in all the uremic patients tested just before a dialysis session, as well as a decrease in RBC Ca ATPase and in the platelet pH. In uremia, significant correlations have been noted between RBC Na and platelet Ca (r = 0.6) or systolic BP (r = 0.45); between platelet Ca and systolic blood pressure (r = 0.8) or diastolic BP (r = 0.5) and between EDLS and RBC Na, Ca or platelet Ca (r = 0.5). Anti-hypertensive treatment has no influence on these parameters. During dialysis, a significant decrease has been noted in RBC Na, Ca, platelet Ca, SBP (only in untreated patients) and EDLS and an increase in RBC Ca ATPase and platelet pH. These modifications are significantly correlated with the weight change. [less ▲]

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See detailPlasma Na-K ATPase inhibitor activity and intracellular ions in volume expanded hypertension
Krzesinski, Jean-Marie ULg; Du, F.; Rorive, Georges ULg

in European Journal of Clinical Investigation (1991), 21

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