Overexpression, purification and characterization of Mycobacterium bovis BCG alcohol dehydrogenase.
; ; et al
in European Journal of Biochemistry (1999), 262(2), 299-307
A previous study of the effect of zinc deprivation on Mycobacterium bovis BCG pointed out the potential importance of an alcohol dehydrogenase for maintaining the hydrophobic character of the cell ... [more ▼]
A previous study of the effect of zinc deprivation on Mycobacterium bovis BCG pointed out the potential importance of an alcohol dehydrogenase for maintaining the hydrophobic character of the cell envelope. In this report, the effect of the overexpression of the M. bovis BCG alcohol dehydrogenase (ADH) in Mycobacterium smegmatis and M. bovis BCG is described. The purification of the enzyme was performed to apparent homogeneity from overexpressing M. bovis BCG cells and its kinetic parameters were determined. The enzyme showed a strong preference for both aromatic and aliphatic aldehydes while the corresponding alcohols were processed 100-1000-fold less efficiently. The best kcat/Km values were found with benzaldehyde > 3-methoxybenzaldehyde > octanal > coniferaldehyde. A phylogenetic analysis clearly revealed that the M. bovis BCG ADH together with the ADHs from Bacillus subtilis and Helicobacter pylori formed a sister group of the class C medium-chain alcohol dehydrogenases, the plant cinnamyl alcohol dehydrogenases (CADs). Comparison of the kinetic properties of our ADH with some related class C enzymes indicated that the mycobacterial enzyme substrate profile resembled that of the CADs involved in plant defence rather than those implicated in lignification. A possible role for the M. bovis BCG ADH in the biosynthesis of the lipids composing the mycobacterial cell envelope is proposed. [less ▲]Detailed reference viewed: 18 (0 ULg)
Overexpression, solubilization and refolding of a genetically engineered derivative of the penicillin-binding protein 3 of Escherichia coli K12.
; Nguyen-Distèche, Martine ; et al
in Molecular Microbiology (1988), 2(4), 519-525
Replacement of the amino-terminal 40-amino-acid region of the 588-amino-acid precursor of the membrane-bound penicillin-binding protein 3 (PBP3) by the decapeptide MKGKEFQAWI was carried out by altering ... [more ▼]
Replacement of the amino-terminal 40-amino-acid region of the 588-amino-acid precursor of the membrane-bound penicillin-binding protein 3 (PBP3) by the decapeptide MKGKEFQAWI was carried out by altering the amino-coding end of the ftsI gene. Insertion of the modified gene into a runaway-replication plasmid under the control of a fused lpp promoter and lac promoter/operator, resulted in the overexpression by Escherichia coli of the modified PBP3 (designated PBP3**) in the cytoplasm. About 80% of the accumulated PBP3** underwent sequestration in the form of insoluble protein granules that were isolated by cell breakage or cell lysis. After selective removal of contaminants by an EDTA-lysozyme/DNase (deoxyribonuclease)/Nonidet extraction, treatment of the granules with guanidinium chloride followed by dialysis against buffer containing 0.5 M NaCl yielded a refolded, water-soluble PBP3**, which, upon chromatography on Superose 12, exhibited the expected 60,000 molecular mass. The refolded PBP3** bound benzylpenicillin in a 1 to 1 molar ratio, was highly sensitive to aztreonam and showed the same degree of thermostability, in terms of penicillin-binding capacity, as the parent, membrane-bound PBP3, suggesting that protein refolding occurred with formation of the correct intramolecular interactions. Two to three mg of refolded PBP3** can be obtained from 1 litre of culture of the overproducing strain. [less ▲]Detailed reference viewed: 9 (1 ULg)
Overgebruik van connectieven in T2 : leerproces of discursieve taalverschillen?
Perrez, Julien ;
Poster (2004)Detailed reference viewed: 5 (0 ULg)
Overgenerality Bias and these Consequences in Borderline Personality Disorder.
Boulanger, Marie ; ; Blairy, Sylvie
Poster (2010, June)
Objective: The present study investigated the Autobiographical Memory (AM) in borderline disorder population. AM is an entity that encompasses the individuals’past personnal experiences. Previous ... [more ▼]
Objective: The present study investigated the Autobiographical Memory (AM) in borderline disorder population. AM is an entity that encompasses the individuals’past personnal experiences. Previous researches have shown disturbances in AM among several psychiatric disorders such as depression, schizophrenia and bipolar disorder. This disturbances take the overgeneral retrieving form. Thus, when patients were asked to retrieve a specific event located in time and place, they recalled an overgeneral event. This deficit is not an isolated phenomen. Indeed the researches showed that AM deficits is related to decreasing of the ability to solve interpersonnal problem (Evans et al., 1992; Goddard et al., 1996) and impairments to project onself into the specific future events (D’argembeau et al., 2008; Williams et al., 1996). Impairments to respond adequately to social problems or to concrete plans for the future create hopelessness and to contribute to suicide attempt (Arie et al., 2008). Given the high risk of suicide or suicide attempts present in the Bordeline Personnality Disorder (BPD), consideration of AM in this population is appropriate. The aim of the present study was investigate the AM, the projection into the future and the problem solving in patients suffering from BPD. Method: 21 subjects BPD and 21 healthy controls participated in this study. First, the participants were asked to complete TeMA (validated French versions of AMT by Neumann & Philippot, 2006). Participants were instructed to generate specific past and future memories in response to cues words. Secondly, they were had to complete the OTT, they were asked to yield the most solutions as possible to daily problems. Finally the depression was controlled as well as neuropsychological variables. Results: The subjects with BPD recalled less specific past events and imagined less specific future events than healthy subjects (t(40) = 2.21, p = .031; t(40) = 3.4, p = .001, respectively). In addition, the number of past and future specific events was marginally correlated (r(42) = .31, p = .051). However, no difference between two groups on OTT and no correlation between past specificity and problem solving emerged. Discussion: As other clinical populations, the subjects with a BPD encounter deficits to retrieve specific past events. Moreover, these impairments are associated with deficits to imagine specific future events. Nevertheless, the ability to generate specific events was not related to the ability to solve problem. The observation of reduced specificity in the generation of autobiographical material is particularly clinically relevant. Indeed, difficulty in imagining the future may contribute to relapse. In conclusion, more systematic measure of this ability should be taken in both research and clinical fields. [less ▲]Detailed reference viewed: 84 (19 ULg)
Overgenerality Memory Style for Past and Future Events and Emotions Related in Bipolar Disorder. What are the Links with Problem Solving and Interpersonal Relationships?
Boulanger, Marie ; ; Blairy, Sylvie
in Psychiatry Research (2013), 210(3), 863-870
This study investigated patients with Bipolar Disorder’s abilities to generate specific past and future events in response to positive and negative cues words as well as emotional intensity related to ... [more ▼]
This study investigated patients with Bipolar Disorder’s abilities to generate specific past and future events in response to positive and negative cues words as well as emotional intensity related to these ones. The relationships between the number of generated specific events cognitive functioning, interpersonal problems and the ability to problem solving were investigated. Nineteen BD and nineteen healthy controls completed a French version of the AMT to evaluate the past and future events recall, in function of their valence, and emotions related. Furthermore, they completed the Optional Thinking Test, the Inventory of Interpersonal Problems and the neuropsychological measures. Compared to healthy controls, BD recollected (1) fewer specific past negative events and (2) fewer future specific positive and negative events furthermore, (3) they felt more emotional intensity related to future events. These results were explained in the light of theoretical models. Finally, specific past memories deficits in BD were linked with issues in problem solving but not with levels of distress arising from interpersonal problems. In view of AM functions in everyday life, all types of deficits should be taken into consideration, and AM remediation envisaged. [less ▲]Detailed reference viewed: 26 (5 ULg)
Overhead athletes and sports performance on the field
Forthomme, Bénédicte ; Crielaard, Jean-Michel ; Croisier, Jean-Louis
in Abstract book du 11th Annual Congress of the European College of Sports Science (ECSSA) (2006)Detailed reference viewed: 22 (7 ULg)
Overhead line galloping : Field experience during one event in Belgium on February 13th, 1997
Lilien, Jean-Louis ; Erpicum, Michel ;
in IWAIS '98, International Conference, Reykjavik,June 1998, Proceedings (1998)Detailed reference viewed: 12 (2 ULg)
Overhead line vertical galloping on bundle configurations : stability criterions and amplitude prédiction.
Lilien, Jean-Louis ;
in Proceedings of the International Conference on OHL design and construction. (1988, November 28)
Galloping of power lines is studied by simulations on multi-spans power lines, including vertical, horizontal an torsional degrees of freedom.Stability conditions, definition of some basic dimensionless ... [more ▼]
Galloping of power lines is studied by simulations on multi-spans power lines, including vertical, horizontal an torsional degrees of freedom.Stability conditions, definition of some basic dimensionless parameters and amplitude evaluation is presented. [less ▲]Detailed reference viewed: 16 (1 ULg)
Overhead lines galloping - Modelisation. Modeling and Simulation of Electrical Machines and Power Systems
Lilien, Jean-Louis ; Ponthot, Jean-Philippe
in TRAN, D. K.; Robert, J. (Eds.) IMACS (1988)
Galloping of power lines is approached by a simple 3 DOF model (one loop).Detailed reference viewed: 43 (0 ULg)
Overhead Lines galloping and turbulent wind
; Lilien, Jean-Louis
in Bulletin Scientifique de l'Association des Ingénieurs Electriciens sortis de l'Institut Electrotechnique Montefiore (1997), 1
Galloping is modelled considering actual turbulent wind using frequency spectrum of the wind and spatial coherence.Detailed reference viewed: 9 (4 ULg)
Overhead power lines real time monitoring : a future trend ?
Lilien, Jean-Louis ; Destiné, Jacques
in Revue d'Electricité et d'Electronique Industrielle (2005), 4
Overview of the needs which exist to monitor overhead lines in real time. Application to ampacity is detailed to determine the load available on a given line at a given time. After an overview of the ... [more ▼]
Overview of the needs which exist to monitor overhead lines in real time. Application to ampacity is detailed to determine the load available on a given line at a given time. After an overview of the existing sensors, a new device is proposed (Ampacimon) and first results are detailed on a 70 kV line. [less ▲]Detailed reference viewed: 94 (8 ULg)
Overhead power lines real time monitoring : a future trend ?
Conference given outside the academic context (2005)
general overview of ampacity problem in power linesDetailed reference viewed: 35 (3 ULg)
Overhead transmission line galloping. A comparative study between 2-DOF and 3-DOF models.
; Lilien, Jean-Louis
in Hogge, Michel; dick, E. (Eds.) Actes du 3ème CNBM (1994, May)
2-D and 3-D models of power line galloping are detailed for multi-span power lines, including suspension insulator movement, tension changes and aerodynamic lift, drag and pitching moment.Detailed reference viewed: 31 (1 ULg)
Overhead transmission lines design. Some mechanical aspects.
Lilien, Jean-Louis ; ; et al
in Proceedings of the ICPST'94 (1994, October)
3-DOF galloping model of power lines.Detailed reference viewed: 36 (1 ULg)
Overheidsopdrachten en andere overheidsovereenkomsten van de lidstaten : 2012 (Kroniek van de Europese rechtspraak
Durviaux, Ann-Lawrence ;
in Marchés et Contrats Publics = Overheidsopdrachten en -Overeenkomsten (2014), 2014/1Detailed reference viewed: 11 (0 ULg)
Overheidsopdrachten en andere overheidsovereenkomsten van de lidstaten: 2010 (Kroniek van de Europese rechtspraak)
in Marchés et Contrats Publics = Overheidsopdrachten en -Overeenkomsten (2011), 2/2011Detailed reference viewed: 20 (3 ULg)
Overlap syndrome consisting of PSC-AIH with concomitant presence of a membranous glomerulonephritis and ulcerative colitis
; BOVY, Christophe ; COIMBRA MARQUES, Carla et al
in World Journal of Gastroenterology (2014), 20(16), 4811-4816
Abstract The association of primary sclerosing cholangitis (PSC) and autoimmune hepatitis (AIH) is known as an overlap syndrome (OS). OS can also be described in the setting of concomitant presence of AIH ... [more ▼]
Abstract The association of primary sclerosing cholangitis (PSC) and autoimmune hepatitis (AIH) is known as an overlap syndrome (OS). OS can also be described in the setting of concomitant presence of AIH and PSC. These diseases can in some cases be associated with ulcerative colitis. In this case report we describe, to our knowledge, the first case in the literature of a young Caucasian male suffering from ulcerative colitis and an overlap syndrome consisting of an association between PSC-AIH, with the concomitant presence of a membranous glomerulonephritis. [less ▲]Detailed reference viewed: 20 (2 ULg)
Overlapping Cre And E Box Motifs In The Enhancer Sequences Of The Bovine Leukemia Virus 5 ' Long Terminal Repeat Are Critical For Basal And Acetylation-Dependent Transcriptional Activity Of The Viral Promoter: Implications For Viral Latency
; ; et al
in Journal of Virology (2004), 78(24),Detailed reference viewed: 38 (10 ULg)
'Overlapping Territories, Intertwined Histories': Cross-Culturality in Caryl Phillips's Crossing the River
in Journal of Commonwealth Literature (1995), 30(1), 55-62Detailed reference viewed: 500 (10 ULg)