A specific method for measurement of equine active myeloperoxidase in biological samples and in in vitro tests

in Journal of Veterinary Diagnostic Investigation (2006), 18(4), 326-334

An original method called SIEFED (specific immunological extraction followed by enzymatic detection) was developed for the specific detection of the activity of equine myeloperoxidase (MPO). The method ... [more ▼]

An original method called SIEFED (specific immunological extraction followed by enzymatic detection) was developed for the specific detection of the activity of equine myeloperoxidase (MPO). The method consists of the extraction of MPO from aqueous solutions by immobilized anti-MPO antibodies followed by washing (to eliminate proteins and interfering molecules) and measurement of MPO activity using a detection system containing a fluorogenic substrate, hydrogen peroxide, and nitrite as reaction enhancer. The SIEFED technique was applied to study active MPO in horse biological fluids and the effects of 2 polyphenolic molecules, curcumin and resveratrol, on MPO activity. The detection limit of the SIEFED was 0.23 mU/ml. The SIEFED exhibited good precision with intra-assay and interassay coefficients of variation below 10% and 20%, respectively, for MPO activities ranging from 0.25 to 6.4 mU/ml. The activity of MPO was generally higher than 1 mU/ml in the fluids collected from horses with inflammatory diseases. Curcumin and resveratrol exerted a dose-dependent inhibition on MPO activity and, as they were removed before the enzymatic detection of MPO, the results suggest a direct drug-nzyme interaction or an enzyme structure modification by the drug. The SIEFED is a new tool that would be useful for specific detection of active MPO in complex media and for selection of MPO activity modulators. [less ▲]

Specific Passive Immunity Reduces the Excretion of Glycoprotein E-Negative Bovine Herpesvirus Type 1 Vaccine Strain in Calves

in Vaccine (2000), 19(9-10), 1013-7

We investigated the excretion of either a glycoprotein E (gE)-negative bovine herpesvirus type 1 (BHV1) vaccine strain or a conventional modified-live vaccine strain in both naive and passively immunised ... [more ▼]

We investigated the excretion of either a glycoprotein E (gE)-negative bovine herpesvirus type 1 (BHV1) vaccine strain or a conventional modified-live vaccine strain in both naive and passively immunised calves. The replication of gE-negative strain was considerably reduced in the maternally immunised calves, in comparison with the non-immune calves. On the other hand, the excretion of the gE-positive conventional vaccine strain was not reduced and even seemed to be prolonged in the presence of maternal antibodies. These results suggest that BHV1 gE may play a role in virus survival in the presence of antibodies. [less ▲]

Specific phosphorylation of Torpedo 43 rapsyn by endogenous kinase(s) with thiamine triphosphate as the phosphate donor

in FASEB Journal (2000), 14

Specific risks of anesthesia in patients with osteoarticular disease

in Acta Anaesthesiologica Belgica (1984), 35(2), 105-121

Anesthesia in patients with either treated or untreated osteoarttcular disorders poses a number of specific problems. The authors deal with risks resulting from mechanical deformities produced by these ... [more ▼]

Anesthesia in patients with either treated or untreated osteoarttcular disorders poses a number of specific problems. The authors deal with risks resulting from mechanical deformities produced by these disorders, by the involvement of a large number of functional systems, by changes in the immune status of such patients and by alterations in the reactions of arthritic patients resulting from prior or concurrent treatments. The authors emphasise the crucial points which require evaluation and observation before and during anesthesia [less ▲]

Specific Structural Features of the N-Acetylmuramoyl-l-Alanine Amidase AmiD from Escherichia coli and Mechanistic Implications for Enzymes of This Family.

in Journal of Molecular Biology (2010), 397

AmiD is the fifth identified N-acetylmuramoyl-l-alanine zinc amidase of Escherichia coli. This periplasmic lipoprotein is anchored in the outer membrane and has a broad specificity. AmiD is capable of ... [more ▼]

AmiD is the fifth identified N-acetylmuramoyl-l-alanine zinc amidase of Escherichia coli. This periplasmic lipoprotein is anchored in the outer membrane and has a broad specificity. AmiD is capable of cleaving the intact peptidoglycan (PG) as well as soluble fragments containing N-acetylmuramic acid regardless of the presence of an anhydro form or not, unlike the four other amidases, AmiA, AmiB, AmiC, and AmpD, which have some specificity. AmiD function is, however, not clearly established but it could be part of the enzymatic machinery involved in the PG turnover in E. coli. We solved three structures of the E. coli zinc amidase AmiD devoid of its lipidic anchorage: the holoenzyme, the apoenzyme in complex with the substrate anhydro-N-acetylmuramic-acid-l-Ala-gamma-d-Glu-l-Lys, and the holoenzyme in complex with the l-Ala-gamma-d-Glu-l-Lys peptide, the product of the hydrolysis of this substrate by AmiD. The AmiD structure shows a relatively flexible N-terminal extension that allows an easy reach of the PG by the enzyme inserted into the outer membrane. The C-terminal domain provides a potential extended geometrical complementarity to the substrate. AmiD shares a common fold with AmpD, the bacteriophage T7 lysozyme, and the PG recognition proteins, which are receptor proteins involved in the innate immune responses of a wide range of organisms. Analysis of the different structures reveals the similarity between the catalytic mechanism of zinc amidases of the AmiD family and the thermolysin-related zinc peptidases. [less ▲]

Specific topics on Bénard-Marangoni instabilities

in Henry, Daniel; Bergeon, Alain (Eds.) Proceedings of the ERCOFTAC and EUROMECH Colloquium 383 on Continuation Methods in Fluid Dynamics (2000)

Specific transcription factors of the tumor BT-474 mammary cells regulate the c-erbB2 gene expression.

Conference (1994, December 22)

Specific transcription factors regulate the c-erbB2 expression in the tumor BT-474 mammary cells.

Conference (1995, March 24)

Specification and verification of a TTP protocol for the conditional access to services

in Formal Methods and their Applications: Telecommunications, VLSI and Real-Time Computerized Control Systems (1996, October)

We use the formal language LOTOS to specify the Equicrypt protocol and verify its robustness to attacks by an intruder. We use the model-based CADP verification tools from the Eucalyptus toolbox to ... [more ▼]

We use the formal language LOTOS to specify the Equicrypt protocol and verify its robustness to attacks by an intruder. We use the model-based CADP verification tools from the Eucalyptus toolbox to discover some successful attacks against this protocol. [less ▲]

Spécification géométrique des concepts architecturaux dans le cadre de la thermique du bâtiment

in Tidafi, T.; Dorta, T. (Eds.) Joindre langages, cultures & visions (2009)

Cette étude s’intéresse à l’assignation des propriétés technologiques des parois dans un modeleur architectural 3D. Nous proposons une méthode originale basée sur le principe de groupements sémantiques de ... [more ▼]

Cette étude s’intéresse à l’assignation des propriétés technologiques des parois dans un modeleur architectural 3D. Nous proposons une méthode originale basée sur le principe de groupements sémantiques de faces par requêtes géométriques. Nous décrivons la méthode et les résultats d’entretiens menés auprès d’architectes pour identifier les groupements pertinents, listons les requêtes géométriques simples pour la sélection de ces groupements et rendons compte de l’efficacité de la méthode sur un cas. Nous concluons sur les avantages et limites de l’approche. [less ▲]