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See detailMid and late Holocene dust deposition in Western Europe: The Misten peat bog (Hautes Fagnes - Belgium)
Allan, Mohammed ULg; Fagel, Nathalie ULg; Le Roux, Gael et al

Poster (2012, June 28)

The Misten peat bog representing 7.5 m of peat accumulation in the Hautes-Fagnes Plateau, Belgium, provides a record of Rare Earth Elements (REE) deposition since more than 7000 years. The analyses of REE ... [more ▼]

The Misten peat bog representing 7.5 m of peat accumulation in the Hautes-Fagnes Plateau, Belgium, provides a record of Rare Earth Elements (REE) deposition since more than 7000 years. The analyses of REE and lithogenic element concentrations, as well as the Nd isotopes, were performed by HR-ICP-MS and MC-ICP-MS, respectively in peat layers previously dated by 210Pb and 14C. REE concentration variations in peat samples are correlated with Ti, Zr and Sc that are lithogenic conservative elements, suggesting that REE are immobile in the studied peat bogs [1] and can be used as tracers of dust deposition. Peat humification, C/N ratio, ash content and bulk density were used to evaluate hydroclimatic conditions. The Nd values show large variability, between +1 to –22, identifying three major sources of dusts falling into the peat: local soils, distal volcanic and desert particles. More recently, industrial emissions provide a fourth source of dusts [2], which is also clearly recorded in the last 200 years of the Misten peat profile. [less ▲]

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See detailMid and late Holocene dust deposition in Western Europe: The Misten peat bog (Hautes Fagnes - Belgium)
Allan, Mohammed ULg; Le Roux, Gael; Piotrowska, Natalia et al

Poster (2012, June)

Multi-proxy climate recorded from peatlands have been used to tracer the climate change during the Holocene. The initiation of peatland may be related to a change to colder and or wetter climate, and the ... [more ▼]

Multi-proxy climate recorded from peatlands have been used to tracer the climate change during the Holocene. The initiation of peatland may be related to a change to colder and or wetter climate, and the variations in peat composition reflect changes in precipitation and temperature. Peat has been used as an archive to reconstruct climate change over the Holocene (e.g., Shotyk et al., 1998; Sapkota et al., 2006). To characterize the climate in Belgium, a 570 cm-long core from Misten peat bog was studied. Several radiocarbon ages allow to define an accurate age model, the peat core represents 5500 years of record. The analyses of REE and lithogenic element contents, as well as the Nd isotopes, were performed by HR-ICP-MS and MC-ICP-MS. Peat humification, C/N ratio, ash content and bulk density were used to evaluate local hydroclimatic conditions. The dust deposition ranges from 0.03 to 4 g m -2 yr -1. As a first observation the highest rates of atmospheric dust deposition correspond to cold periods. The Nd values show large variability, between +1 to –22, identifying three major sources of dusts falling into the peat: local soils, distal volcanic and desert particles. Further studies are in progress to better identify the main forcing factor on the evolution of the atmospheric dust deposition over the Holocene. [less ▲]

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See detailMid infrared and fluorescence spectroscopies coupled with factorial discriminant analysis technique to identify sheep milk from different feeding systems
Karoui, Romdhane; Hammami, Moncef; Rouissi, Hamadi et al

in Food Chemistry (2011), 127(2), 743-748

Mid infrared spectroscopy (MIR) combined with multivariate data analysis was used to discriminate between ewes milk samples according to their feeding systems (controls, ewes fed scotch bean and ewes fed ... [more ▼]

Mid infrared spectroscopy (MIR) combined with multivariate data analysis was used to discriminate between ewes milk samples according to their feeding systems (controls, ewes fed scotch bean and ewes fed soybean). The MIR spectra were scanned throughout the first 11 weeks of the lactation stage. When factorial discriminant analysis (FDA) with leave one-out cross-validation was applied, separately, to the three spectral regions in the MIR (i.e. 3000-2800, 1700-1500 and 1500-900 cm(-1)), the classification rate was not satisfactory. Therefore, the first principal component (PCs) scores (corresponding to 3, 10 and 10 for, respectively, the 3000-2800, 1700-1500 and 1500-900 cm(-1)) of the principal component analysis (PCA) extracted from each of the data sets were pooled (concatenated) into a single matrix and analysed by FDA. Correct classification amounting to 71.7% was obtained. Finally, the same procedure was applied to the MIR and fluorescence data sets and 98% of milk samples were found to be correctly classified. Milk samples belonging to control and soybean groups were 100% correctly classified. Regarding milk samples originating from the scotch bean group, only 2 out of 33 samples were misclassified. It was concluded that concatenation of the data sets collected from the two spectroscopic techniques is an efficient tool for authenticating milk samples according to their feeding systems, regardless of the lactation stage. (C) 2011 Published by Elsevier Ltd. [less ▲]

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See detailMid-Famennian cryptalgal-sponge crinoidal mud mounds and oolitic ironstones of the Limbourg-Dolhain area (Verviers Synclinorium, Eastern Belgium): Stop 2.
Dreesen, Roland; Marion, Jean-Marc ULg; Poty, Edouard ULg

Scientific conference (1993, June 09)

After the mass extinction of colonial reefs in the Ardennes at the Frasnian-Famennian boundary, (crypt)algal-sponge-crinoidal mud mounds suddenly appeared during the mid-Famennian time (Early-Late ... [more ▼]

After the mass extinction of colonial reefs in the Ardennes at the Frasnian-Famennian boundary, (crypt)algal-sponge-crinoidal mud mounds suddenly appeared during the mid-Famennian time (Early-Late marginifera Zone) in the Verviers Synclinorium (Eastern Belgium). These carbonate buildups represent the first reported occurrence of mud mounds in the latest Upper Devonian of Western Europe. The mounding sites for the mud mounds (locally known as "the Marbre rouge à crinoïdes de Baelen") are developed on a block-faulted basement. [less ▲]

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See detailMid-FIR Properties of ELAIS Sources
Marquez, I.; Masegosa, J.; Morel, Thierry ULg et al

in The Promise of the Herschel Space Observatory (2001, July 01)

We present the properties of all the galaxies detected by ISO at 7, 15 and 90 microns in ELAIS northern fields. The spectral energy distribution (SED) of those 20 galaxies with IRAS detections can ... [more ▼]

We present the properties of all the galaxies detected by ISO at 7, 15 and 90 microns in ELAIS northern fields. The spectral energy distribution (SED) of those 20 galaxies with IRAS detections can generally be well fitted by a predominant cirrus component plus a modest starburst contribution. Follow-up spectroscopy has shown that all the objects are emission-line galaxies but without a very intense star formation event. Most of the galaxies analyzed by means of optical R band photometry result to host an important exponential disk component, in good agreement with the SED IR modelling. We note that galaxies with morphological signs of perturbations seem to show slightly higher f[SUB]15[/SUB]/f[SUB]6.7[/SUB] ratios, indicating that star formation could be more important in them. One of the objects is a broad-line, radio-quiet quasar at z=1.099; its spectral energy distribution indicates that it is a hyperluminous infrared galaxy (HLIG), the first HLIG detected in the ELAIS areas. [less ▲]

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See detailMid-infrared extinction by sulfate aerosols from the Mt Pinatubo eruption
Rinsland, C. P.; Yue, G. K.; Gunson, M. R. et al

in Journal of Quantitative Spectroscopy & Radiative Transfer (1994), 52(3), 241-252

Quantitative measurements of the wavelength dependence of aerosol extinction in the 750–3400 cm-1 spectral region have been derived from 0.01 cm-1 resolution stratospheric solar occultation spectra ... [more ▼]

Quantitative measurements of the wavelength dependence of aerosol extinction in the 750–3400 cm-1 spectral region have been derived from 0.01 cm-1 resolution stratospheric solar occultation spectra recorded by the ATMOS (Atmospheric Trace Molecule Spectroscopy) Fourier transform spectrometer about 912 months after the Mt Pinatubo volcanic eruption. Strong, broad aerosol features have been identified near 900, 1060, 1720, and 2900 cm-1 below a tangent height of ~30 km. Aerosol extinction measurements derived from ~0.05 cm-1 wide microwindows nearly free of telluric line absorption in the ATMOS spectra are compared with transmission calculations derived from aerosol size distribution profiles retrieved from correlative SAGE (Stratospheric Aerosol and Gas Experiment) II visible and near i.r. extinction measurements, seasonal and zonally averaged H2SO4 aerosol weight percentage profiles, and published sulfuric acid optical constants derived from room temperature laboratory measurements. The calculated shapes and positions of the aerosol features are generally consistent with the observations, thereby confirming that the aerosols are predominantly concentrated H2SO4-H2O droplets, but there are significant differences between the measured and calculated wavelength dependences of the aerosol extinction. We attribute these differences as primarily the result of errors in the calculated low temperature H2SO4-H2O optical constants. Errors in both the published room temperature optical constants and the limitations of the Lorentz-Lorenz relation are likely to be important. [less ▲]

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See detailMid-infrared High-contrast Imaging of HD 114174 B: An Apparent Age Discrepancy in a "Sirius-like" Binary System
Matthews, Christopher T.; Crepp, Justin R.; Skemer, Andrew et al

in Astrophysical Journal Letters (2014), 783

We present new observations of the faint "Sirius-like" companion discovered to orbit HD 114174. Previous attempts to image HD 114174 B at mid-infrared wavelengths using NIRC2 at Keck have resulted in a ... [more ▼]

We present new observations of the faint "Sirius-like" companion discovered to orbit HD 114174. Previous attempts to image HD 114174 B at mid-infrared wavelengths using NIRC2 at Keck have resulted in a non-detection. Our new L'-band observations taken with the Large Binocular Telescope and L/M-band InfraRed Camera recover the companion (ΔL = 10.15 ± 0.15 mag, ρ = 0.''675 ± 0.''016) with a high signal-to-noise ratio (10σ). This measurement represents the deepest L' high-contrast imaging detection at subarcsecond separations to date, including extrasolar planets. We confirm that HD 114174 B has near-infrared colors consistent with the interpretation of a cool white dwarf (WD; J - L' = 0.76 ± 0.19 mag, K - L' = 0.64 ± 0.20). New model fits to the object's spectral energy distribution indicate a temperature T [SUB]eff[/SUB] = 4260 ± 360 K, surface gravity log g = 7.94 ± 0.03, a cooling age t[SUB]c[/SUB] ≈ 7.8 Gyr, and mass M = 0.54 ± 0.01 M [SUB]⊙[/SUB]. We find that the cooling ages given by theoretical atmospheric models do not agree with the age of HD 114174 A derived from both isochronological and gyrochronological analyses. We speculate on possible scenarios to explain the apparent age discrepancy between the primary and secondary. HD 114174 B is a nearby benchmark WD that will ultimately enable a dynamical mass estimate through continued Doppler and astrometric monitoring. Efforts to characterize its physical properties in detail will test theoretical atmospheric models and improve our understanding of WD evolution, cooling, and progenitor masses. [less ▲]

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See detailMid-infrared microlensing of accretion disc and dusty torus in quasars: effects on flux ratio anomalies
Sluse, Dominique ULg; Kishimoto, M.; Anguita, T. et al

in Astronomy and Astrophysics (2013), 553

Multiply-imaged quasars and active galactic nuclei (AGNs) observed in the mid-infrared (MIR) range are commonly assumed to be unaffected by the microlensing produced by the stars in their lensing galaxy ... [more ▼]

Multiply-imaged quasars and active galactic nuclei (AGNs) observed in the mid-infrared (MIR) range are commonly assumed to be unaffected by the microlensing produced by the stars in their lensing galaxy. In this paper, we investigate the validity domain of this assumption. Indeed, that premise disregards microlensing of the accretion disc in the MIR range, and does not account for recent progress in our knowledge of the dusty torus, which has unveiled relatively compact dust emission. To simulate microlensing, we first built a simplified image of the quasar composed of (i) an accretion disc whose size is based on accretion disc theory, and (ii) a larger ring-like torus whose radius is guided by interferometric measurements in nearby AGNs. The mock quasars are created in the 10[SUP]44.2[/SUP]-10[SUP]46[/SUP] erg/s (unlensed) luminosity range, which is typical of known lensed quasars, and are then microlensed using an inverse ray-shooting code. We simulated the wavelength dependence of microlensing for different lensed image types and for various fractions of compact objects in the lens. This allows us to derive magnification probabilities as a function of wavelength, as well as to calculate the microlensing-induced deformation of the spectral energy distribution of the lensed images. We find that microlensing variations as large as 0.1 mag are very common at 11 μm (typically rest-frame 4 μm). The main signal comes from microlensing of the accretion disc, which may be significant even when the fraction of flux from the disc is as small as 5% of the total flux. We also show that the torus of sources with L[SUB]bol[/SUB] ≲ 10[SUP]45[/SUP] erg/s is expected to be noticeably microlensed. Microlensing may thus be used to get insight into the rest near-infrared inner structure of AGNs. Finally, we investigate whether microlensing in the mid-infrared can alter the so-called R[SUB]cusp[/SUB] relation that links the fluxes of the lensed images triplet produced when the source lies close to a cusp macro-caustic. This relation is commonly used to identify massive (dark-matter) substructures in lensing galaxies. We find that significant deviations from R[SUB]cusp[/SUB] may be expected, which means that microlensing can explain part of the flux ratio problem. Microlensing maps and source profiles used for our simulations are only available in electronic form at the CDS via anonymous ftp to <A href="http://cdsarc.u-strasbg.fr>cdsarc.u-strasbg.fr</A> (ftp://130.79.128.5) or via <a href=">http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/553/A53</A> [less ▲]

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See detailMid-infrared prediction of bovine milk fatty acids across multiple breeds, production systems, and countries
Soyeurt, Hélène ULg; Dehareng, ; Gengler, Nicolas ULg et al

in Journal of Dairy Science (2011), 94

Increasing consumer concern exists over the relationship between food composition and human health. Because of the known effects of fatty acids on human health, the development of a quick, inexpensive ... [more ▼]

Increasing consumer concern exists over the relationship between food composition and human health. Because of the known effects of fatty acids on human health, the development of a quick, inexpensive, and accurate method to directly quantify the fatty acid (FA) composition in milk would be valuable for milk processors to develop a payment system for milk pertinent to their customer requirements and for farmers to adapt their feeding systems and breeding strategies accordingly. The aim of this study was (1) to confirm the ability of mid-infrared spectrometry (MIR) to quantify individual FA content in milk by using an innovative procedure of sampling (i.e., samples were collected from cows belonging to different breeds, different countries, and in different production systems); (2) to compare 6 mathematical methods to develop robust calibration equations for predicting the contents of individual FA in milk; and (3) to test interest in using the FA equations developed in milk as basis to predict FA content in fat without corrections for the slope and the bias of the developed equations. In total, 517 samples selected based on their spectral variability in 3 countries (Belgium, Ireland, and United Kingdom) from various breeds, cows, and production systems were analyzed by gas chromatography (GC). The samples presenting the largest spectral variability were used to calibrate the prediction of FA by MIR. The remaining samples were used to externally validate the 28 FA equations developed. The 6 methods were (1) partial least squares regression (PLS); (2) PLS + repeatability file (REP); (3) first derivative of spectral data + PLS; (4) first derivative + REP + PLS; (5) second derivative of spectral data + PLS; and (6) second derivative + REP + PLS. Methods were compared on the basis of the crossvalidation coefficient of determination (R2cv), the ratio of standard deviation of GC values to the standard error of cross-validation (RPD), and the validation coefficient of determination (R2v). The third and fourth methods had, on average, the highest R2cv, RPD, and R2v. The final equations were built using all GC and the best accuracy was observed for the infrared predictions of C4:0, C6:0, C8:0, C10:0, C12:0, C14:0, C16:0, C18:0, C18:1 trans, C18:1 cis-9, C18:1 cis, and for some groups of FA studied in milk (saturated, monounsaturated, unsaturated, short-chain, medium-chain, and long-chain FA). These equations showed R2cv greater than 0.95. With R2cv equal to 0.85, the MIR prediction of polyunsaturated FA could be used to screen the cow population. As previously published, infrared predictions of FA in fat are less accurate than those developed from FA content in milk (g/dL of milk) and no better results were obtained by using milk FA predictions if no corrections for bias and slope based on reference milk samples with known contents of FA were used. These results indicate the usefulness of equations with R2cv greater than 95% in milk payment systems and the usefulness of equations with R2cv greater than 75% for animal breeding purposes. [less ▲]

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See detailMid-infrared prediction of cheese yield from milk and its genetic variability in first-parity cows
Colinet, Frédéric ULg; Troch, Thibault ULg; Vanden Bossche, Sandrine et al

in Book of Abstracts of the 64th Annual Meeting of the European Federation of Animal Science (2013, August)

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See detailMid-infrared prediction of cheese yield from milk and its genetic variability in first-parity cows
Colinet, Frédéric ULg; Troch, Thibault ULg; Vanden, Bossche et al

Conference (2013, August 29)

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See detailMid-infrared prediction of milk titratable acidity and its genetic variability in first-parity cows
Colinet, Frédéric ULg; Vanlierde, Amélie ULg; Vanden Bossche, sandrine ULg et al

in Book of Abstracts of the 63rd Annual Meeting of the European Federation of Animal Science (2012, August)

Coagulation of milkhas a direct effect on cheese yield. Among several parameters, titratable acidity of milk (TA) influences all the phases of milk coagulation. In order to study the genetic variability ... [more ▼]

Coagulation of milkhas a direct effect on cheese yield. Among several parameters, titratable acidity of milk (TA) influences all the phases of milk coagulation. In order to study the genetic variability of this trait on a large scale, mid-infrared (MIR) chemometric methods were used to predict TA. A total of 507 milk samples collected in the Walloon Region of Belgium from individual cows were analyzed using a MIR spectrometer. TA was recorded as Dornic degree. An equation to predict TA from milk MIR spectrum was developed using partial least squared regression after a first derivative pre-treatment applied to the spectra to correct the baseline drift. During the calibration process, 45 outliers were detected and removed from the calibration set. The TA mean of the final calibration set was 16.62 (standard deviation (SD) = 1.80). The coefficient of determination (R²) was 0.82 for the calibration with a standard error (SE) of 0.76. A cross-validation (cv) was performed (R²cv = 0.81 with SEcv = 0.80). This equation was then applied on the spectral database generated during the Walloon routine milk recording. The variances components were estimated by REML using single-trait random regression animal test-day model. The dataset used included 33,717 records from 9,191 Holstein first-parity cows; the TA mean was 17.05 (SD = 1.35) and TA ranged from 12.83 to 20.87. Estimated daily heritabilities ranged from 0.43 at 5th day in milk to 0.59 at 215th day in milk indicating potential of selection. Further research will study phenotypic and genetic correlations between TA and milk production traits. [less ▲]

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See detailMid-infrared prediction of milk titratable acidity and its genetic variability in first-parity cows
Colinet, Frédéric ULg; Vanlierde, Amélie ULg; Vanden Bossche, sandrine ULg et al

Conference (2012, August 27)

Coagulation of milkhas a direct effect on cheese yield. Among several parameters, titratable acidity of milk (TA) influences all the phases of milk coagulation. In order to study the genetic variability ... [more ▼]

Coagulation of milkhas a direct effect on cheese yield. Among several parameters, titratable acidity of milk (TA) influences all the phases of milk coagulation. In order to study the genetic variability of this trait on a large scale, mid-infrared (MIR) chemometric methods were used to predict TA. A total of 507 milk samples collected in the Walloon Region of Belgium from individual cows were analyzed using a MIR spectrometer. TA was recorded as Dornic degree. An equation to predict TA from milk MIR spectrum was developed using partial least squared regression after a first derivative pre-treatment applied to the spectra to correct the baseline drift. During the calibration process, 45 outliers were detected and removed from the calibration set. The TA mean of the final calibration set was 16.62 (standard deviation (SD) = 1.80). The coefficient of determination (R²) was 0.82 for the calibration with a standard error (SE) of 0.76. A cross-validation (cv) was performed (R²cv = 0.81 with SEcv = 0.80). This equation was then applied on the spectral database generated during the Walloon routine milk recording. The variances components were estimated by REML using single-trait random regression animal test-day model. The dataset used included 33,717 records from 9,191 Holstein first-parity cows; the TA mean was 17.05 (SD = 1.35) and TA ranged from 12.83 to 20.87. Estimated daily heritabilities ranged from 0.43 at 5th day in milk to 0.59 at 215th day in milk indicating potential of selection. Further research will study phenotypic and genetic correlations between TA and milk production traits. [less ▲]

Detailed reference viewed: 101 (40 ULg)
See detailMid-infrared predictions of cheese yield from bovine milk
Vanlierde, Amélie ULg; Soyeurt, Hélène ULg; Anceau, Christine ULg et al

in Book of Abstracts of the 62nd Annual Meeting of the European Association for Animal Production (2011, August)

Economically, cheese yield (CY) is very important. Todate, empirical or theoretical formulae allow estimating the theoretical CY from milk fat and casein or protein content of milk. It would be ... [more ▼]

Economically, cheese yield (CY) is very important. Todate, empirical or theoretical formulae allow estimating the theoretical CY from milk fat and casein or protein content of milk. It would be interesting to predict CY during milk recording directly without the need to estimate milk components. Through the BlueSel project, 157 milk samples were collected in Wallonia from individual cows and analyzed using a mid-infrared (MIR) MilkoScanFT6000 spectrometer. Individual laboratory cheese yields (ILCY) were determined for each sample and expressed as g of dry coagulum/100 g of milk dry matter. An equation to predict ILCY from MIR was developed using partial least squared regression (Winisi III). A first derivative pre-treatment of spectra was used to correct the baseline drift. To improve the repeatability of the spectral data, a file which contained the spectra of samples analyzed on 5 spectrometers was used during the calibration. During calibration, 23 outliers were detected a nd removed from the calibration set. The ILCY mean of the final calibration set was 63.9% with a SD of 11.2%. The calibration (C) coefficient of determination (R²) was equal to 0.76 with a standard error (SE) of calibration of 5.5%. A full cross-validation (CV) was preformed to assess the robustness. R²cv was 0.72 with a SECV of 6.0%. The similarity between R²c and R²cv as well as between SEC and SECV permits to consider robustness of the developed equation as good. Even if it is planned to improve the equation with additional samples, this first equation will permit to study ILCY in the Walloon dairy cattle. [less ▲]

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See detailMid-infrared predictions of cheese yield from bovine milk
Vanlierde, Amélie ULg; Soyeurt, Hélène ULg; Anceau, Christine ULg et al

Conference (2011, August 31)

Economically, cheese yield (CY) is very important. Todate, empirical or theoretical formulae allow estimating the theoretical CY from milk fat and casein or protein content of milk. It would be ... [more ▼]

Economically, cheese yield (CY) is very important. Todate, empirical or theoretical formulae allow estimating the theoretical CY from milk fat and casein or protein content of milk. It would be interesting to predict CY during milk recording directly without the need to estimate milk components. Through the BlueSel project, 157 milk samples were collected in Wallonia from individual cows and analyzed using a mid-infrared (MIR) MilkoScanFT6000 spectrometer. Individual laboratory cheese yields (ILCY) were determined for each sample and expressed as g of dry coagulum/100 g of milk dry matter. An equation to predict ILCY from MIR was developed using partial least squared regression (Winisi III). A first derivative pre-treatment of spectra was used to correct the baseline drift. To improve the repeatability of the spectral data, a file which contained the spectra of samples analyzed on 5 spectrometers was used during the calibration. During calibration, 23 outliers were detected a nd removed from the calibration set. The ILCY mean of the final calibration set was 63.9% with a SD of 11.2%. The calibration (C) coefficient of determination (R²) was equal to 0.76 with a standard error (SE) of calibration of 5.5%. A full cross-validation (CV) was preformed to assess the robustness. R²cv was 0.72 with a SECV of 6.0%. The similarity between R²c and R²cv as well as between SEC and SECV permits to consider robustness of the developed equation as good. Even if it is planned to improve the equation with additional samples, this first equation will permit to study ILCY in the Walloon dairy cattle. [less ▲]

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See detailMid-infrared predictions of fatty acids in bovine milk : final results of the RobustMilk project
Soyeurt, Hélène ULg; McParland, Sinead; Berry, Donagh et al

in Book of Abstracts of the 63rd Annual Meeting of the European Federation of Animal Science (2012, August)

The development of mid-infrared equations to predict the milk fatty acid (FA) content of milk allows prompt analysis of large numbers of samples and was one of the aims of the RobustMilk project. Data on ... [more ▼]

The development of mid-infrared equations to predict the milk fatty acid (FA) content of milk allows prompt analysis of large numbers of samples and was one of the aims of the RobustMilk project. Data on MIR spectra and FA from multiple countries, production systems, and breeds were used to develop equations to predict milk FA. The calibration set contained 1,776 spectrally different English, Irish, and Belgian milk samples collected for over 6 years. FA were quantified by gas chromatography (GC). Equations were built using partial least squares regression after a first derivative pretreatment applied to the spectral data. The robustness of the developed equations was assessed by cross-validation (CV) using 50 groups from the calibration set. The coefficient of determination (R²) obtained after CV ranged between 0.7101 for the total content of C18:2 and 0.9993 for the saturated FA group. The standard error of CV ranged between 0.0028 and 0.0998 g/dl of milk. Generally, the group or individual FA having the highest content in milk had the highest R²cv. The results obtained in this study confirmed the usefulness of MIR spectra to robustly quantify the FA content of milk permitting the use of these equations by milk laboratories in UK, Belgium or Ireland. Therefore, these equations could be used to develop selection or management tools for dairy farmers in order to improve the nutritional and environmental quality of milk based on the knowledge of the FA composition of their milk. [less ▲]

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See detailMid-infrared predictions of fatty acids in bovine milk : final results of the RobustMilk project
Soyeurt, Hélène ULg; McParland, Sinead; Berry, Donagh et al

Poster (2012, August 28)

The development of mid-infrared equations to predict the milk fatty acid (FA) content of milk allows prompt analysis of large numbers of samples and was one of the aims of the RobustMilk project. Data on ... [more ▼]

The development of mid-infrared equations to predict the milk fatty acid (FA) content of milk allows prompt analysis of large numbers of samples and was one of the aims of the RobustMilk project. Data on MIR spectra and FA from multiple countries, production systems, and breeds were used to develop equations to predict milk FA. The calibration set contained 1,776 spectrally different English, Irish, and Belgian milk samples collected for over 6 years. FA were quantified by gas chromatography (GC). Equations were built using partial least squares regression after a first derivative pretreatment applied to the spectral data. The robustness of the developed equations was assessed by cross-validation (CV) using 50 groups from the calibration set. The coefficient of determination (R²) obtained after CV ranged between 0.7101 for the total content of C18:2 and 0.9993 for the saturated FA group. The standard error of CV ranged between 0.0028 and 0.0998 g/dl of milk. Generally, the group or individual FA having the highest content in milk had the highest R²cv. The results obtained in this study confirmed the usefulness of MIR spectra to robustly quantify the FA content of milk permitting the use of these equations by milk laboratories in UK, Belgium or Ireland. Therefore, these equations could be used to develop selection or management tools for dairy farmers in order to improve the nutritional and environmental quality of milk based on the knowledge of the FA composition of their milk. [less ▲]

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See detailMid-infrared predictions of lactoferrin content in bovine milk
Soyeurt, Hélène ULg; Bastin, Catherine ULg; Colinet, Frédéric ULg et al

in Journal of Dairy Science (2011), 94(E-suppl.1), 714

Lactoferrin (LF) is a glycoprotein present in milk and active in the immune system of cows and humans. Therefore, an inexpensive and rapid analysis to quantify this protein is desirable. A previous study ... [more ▼]

Lactoferrin (LF) is a glycoprotein present in milk and active in the immune system of cows and humans. Therefore, an inexpensive and rapid analysis to quantify this protein is desirable. A previous study reported the potential to quantify LF from the mid-infrared (MIR) spectrometry from 69 milk samples. Through the European RobustMilk project (www.robustmilk.eu), 3,606 milk samples were collected in Belgium, Ireland, and Scotland from individual cows and analyzed using a MIR MilkoScanFT6000 spectrometer. Milk LF content was quantified using ELISA in duplicate. Average ELISA data with a CV lower than 5% were used. After the detection of spectral and ELISA outliers, the calibration set contained 2,499 samples. An equation to predict LF content from MIR was developed using partial least squared regression. A first derivative pre-treatment of spectra was used to correct the baseline drift. To improve the repeatability of the spectral data, a file which contained the spectra of samples analyzed on 5 spectrometers was used during the calibration. The lactoferrin mean was 159.28 mg/l of milk with a SD of 97.21 mg/l of milk. The calibration (C) coefficient of determination (R2) was equal to 0.73 with a standard error (SE) of calibration of 50.54 mg/l of milk. A cross-validation (CV) was used to assess the robustness of the equation. R2 CV was 0.72 with a SE-CV of 51.16 mg/l of milk. An external validation (V) was conducted on 150 milk samples collected in Belgium. The SE of prediction (SEP) was 59.17 mg/L of milk. The similarity between R2 C and R2CV as well as between SE-C and SE-CV and between SE-CV and SEP confirms the equations developed are robust. The correlation between predicted and measured LF values was 0.71. This lower value compared with the one obtained from the calibration set (0.85) could be explained by the low ELISA reproducibility (16.24% ± 25.51%). If the developed equation is used to clean the validation data set, a total of 16 samples can be deleted. The validation coefficient for these 134 samples increased to 0.82. From these results, the developed equation could be used for screening the dairy cow population for breeding purposes. [less ▲]

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