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See detailORF9p phosphorylation by ORF47p is crucial for the formation and egress of the Varicella-zoster virus (VZV) viral particles.
Riva, Laura ULg; Thiry, Marc ULg; BONTEMS, Sébastien ULg et al

in Journal of Virology (2013), 87(5), 2868-2881

The role of the tegument during the herpesvirus lytic cycle is still not clearly established, particularly at the late phase of infection, when the newly produced viral particles need to be fully ... [more ▼]

The role of the tegument during the herpesvirus lytic cycle is still not clearly established, particularly at the late phase of infection, when the newly produced viral particles need to be fully assembled before being released from the infected cell. The Varicella-zoster virus (VZV) protein coded by ORF9 (ORF9p) is an essential tegument protein and, even though its mRNA is the most expressed during the productive infection, little is known about its functions. Using a GalK positive/negative selection technique, we modified a BAC containing the complete VZV genome creating viruses expressing mutant versions of ORF9p.We showed that ORF9p is hyper-phosphorylated during the infection, especially through its interaction with the viral Ser/Thr kinase ORF47p; we identified a consensus site within ORF9p recognized by ORF47p and demonstrated its importance for ORF9p phosphorylation. Strikingly, an ultra-structural analysis revealed that the mutation of this consensus site (Glutamate 85 to Arginine) strongly affects viral assembly and release, reproducing ORF47 kinase dead VZV phenotype. It also slightly diminishes the infectivity towards immature dendritic cells. Taken together, our results identify ORF9p as a new viral substrate of ORF47p and suggest a determinant role of this phosphorylation for viral infectivity, especially during the process of viral particle formation and egress. [less ▲]

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See detailORF9p: a new player in the Varicella-Zoster Virus egress
Riva, Laura ULg

Doctoral thesis (2013)

Le virus de la Varicelle et du Zona (VZV) appartient à la famille des herpesviridae, virus enveloppés de grande taille caractérisés par un génome à ADN. Tout comme les autres membres de sa famille, son ... [more ▼]

Le virus de la Varicelle et du Zona (VZV) appartient à la famille des herpesviridae, virus enveloppés de grande taille caractérisés par un génome à ADN. Tout comme les autres membres de sa famille, son cycle viral est complexe et beaucoup dʼétapes doivent encore être élucidées. Parmi les 72 ORFs, lʼORF9 est le plus fortement exprimé au cours de lʼinfection. La protéine codée par ce gène est essentielle pour la réplication du VZV. Elle est conservée parmi les alphaherpesvirus et appartient au tegument du virus. Cependant, jusquʼà présent aucune fonction nʼa été décrite pour lʼORF9p ou pour ses homologues. Le but principal de notre projet de recherche était de caractériser lʼORF9p avec une attention particulière sur la détermination de son rôle biologique dans lʼinfection. Grâce à une technique de recombinaison homologue en deux étapes, nous avons pu insérer différents mutants de lʼORF9 dans un BAC contenant le génome complet du VZV et analyser les phénotypes des virus correspondants ainsi produits. Nous avons montré que lʼORF9p est fortement phosphorylée au cours de lʼinfection, principalement par la kinase virale ORF47p qui reconnaît le site consensus 84SEDD87 dans la séquence de cette protéine. Nous avons par ailleurs montré lʼimportance du glutamate en position 85 pour la phosphorylation de lʼORF9p ainsi que pour lʼenveloppement secondaire et la production de particules virales libres complètes. De plus, la région acide 85EDDFEDIDE93 a été identifiée comme importante pour lʼinfectivité virale. Dans un contexte où cette région est délétée, un défaut dʼenveloppement est observé et la glycoprotéine gE, interactant de lʼORF9p, semble sʼaccumuler dans les membranes intracellulaires, suggérant une incapacité de cette glycoprotéine à rejoindre la membrane plasmique. Après un screening par double hybride en levure, nous avons mis en évidence plusieurs partenaires cellulaires de lʼORF9p. Parmi ceux- ci se trouve la protéine AP-1μ1, connue pour être responsable du transport vésiculaire dépendant de la clathrine entre le Trans-Golgi Network et les endosomes. Lʼinteraction avec cette protéine cellulaire a été confirmée dans un contexte infectieux. Ce travail met donc en évidence pour la première fois lʼimplication de lʼORF9p dans lʼassemblage et la sortie du VZV, en proposant deux modèles : 1) lʼORF9p serait responsable de lʼenvoi de gE à la surface cellulaire via son interaction avec cette glycoprotéine dʼune part et AP-1μ1 dʼautre part ; 2) lʼORF9p serait responsable du processus dʼenveloppement secondaire, nécessaire pour la production de particules virales libres. [less ▲]

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See detailOrfèvrerie mosane XIIe & XIIIe siècle. L'oeuvre de la Meuse
George, Philippe ULg

Book published by Trésor de Liège (2014)

L'ouvrage rassemble les contributions originales sur des oeuvres d'orfèvrerie mosane et septentrionale des XIIe et XIIIe siècles de musées européens et américains par les conservateurs des collections et ... [more ▼]

L'ouvrage rassemble les contributions originales sur des oeuvres d'orfèvrerie mosane et septentrionale des XIIe et XIIIe siècles de musées européens et américains par les conservateurs des collections et des scientifiques de plusieurs universités [less ▲]

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See detailOrfèvrerie mosane, liégeoise, namuroise, montoise … orfèvrerie wallonne
George, Philippe ULg

in Maquet, Julien (Ed.) Le savoir-faire wallon au fil du temps. Le bassin mosan, berceau de techniques de pointe, Les dossiers de l’Institut du Patrimoine Wallon (2011)

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See detailLes orfèvres mosans devant l'histoire (XIe-XIIIe siècle)
George, Philippe ULg; Stiennon, Jacques ULg; Kupper, Jean-Louis ULg

in Bulletin de la Société Royale Le Vieux-Liège (2000)

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See detailOrgan donation after euthanasia on specific patients' request in Belgium
Ysebaert, Y; DETRY, Olivier ULg; Mikhalski, D et al

Conference (2015, March 28)

Euthanasia is banned in almost countries of the world, although in 2002 Belgium legalized it for adults under strict conditions (in a similar way after The Netherlands in 2001. The patient must be in a ... [more ▼]

Euthanasia is banned in almost countries of the world, although in 2002 Belgium legalized it for adults under strict conditions (in a similar way after The Netherlands in 2001. The patient must be in a medically futile condition, of constant and unbearable physical or mental suffering that cannot be alleviated, resulting from a serious and incurable disorder caused by illness or accident. If the person is not in the terminal phase of his illness, the 2 doctors must consult with a third doctor, either a psychiatrist or a specialist in the disease concerned. From 2005 till 2014 more than 25 patients, suffering from diverse neuropsychiatric diseases, got their request for euthanasia granted, and subsequently asked spontaneously for the possibility of organ donation. The involved physicians, the transplant teams and the Institutional Ethics Committees, had the well-discussed opinion that this strong request for organ donation after euthanasia could not be denied. A clear separation between the euthanasia request, the euthanasia procedure and the organ procurement procedure was judged necessary. After extensive preparation, finally, in Belgium, 17 patients got their wish for organ donation after euthanasia fulfilled, in several academic or non-academic hospitals and in different regions : Antwerpen 6, Leuven 5, Liege 2, Namur 1, Turnhout 1, and Brussels 2. Several requests and negotiations were started for other patients but ultimately failed due to patients’ personal choices (e.g. patient wanted finally to die at home) or logistically reasons (e.g. who would fulfil the euthanasia in case all involving doctors were not employed or connected to a hospital). The euthanasia procedure was carried out by three physicians in the neighborhood of the operating room. After clinical diagnosis of cardiac death, the procurement team came in and performed the organ procurement similar as in a DCD type III procedure. The liver, two kidneys and sometimes lungs and pancreatic islets could be successfully recovered and transplanted, after organ allocation via Eurotransplant. Transplant centers were informed about the nature of the case and the elements of organ procurement. There was primary function of all organs. The possibility of organ donation after their euthanasia provides a very much improved self-image of these patients, and adds something really positive to the euthanasia procedure, and is very well appreciated by the requesting patients, relatives, patient and professional organisations and public media. Some practical and ethical issues still have to be discussed to allow expansion of this possibility of organ donation. [less ▲]

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See detailOrgan donation after physician-assisted death
Detry, Olivier ULg; Laureys, Steven ULg; Faymonville, Marie ULg et al

in Transplant International (2008), 21(9), 915

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See detailOrgan donation in Belgium 2009: 20% of donation after cardiocirculatory death.
Detry, Olivier ULg; Troisi, Roberto

in Transplantation Proceedings (2010), 42(10), 4365-6

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See detailOrgan donors with primary central nervous system tumor
Detry, Olivier ULg; Honore, Pierre ULg; Hans, Marie-France ULg et al

in Transplantation (2000), 70(1), 244-8251-2

Patients with primary central nervous system (CNS) tumor have been accepted for organ donation because these tumors very rarely spread outside the CNS. However several case reports of CNS tumor ... [more ▼]

Patients with primary central nervous system (CNS) tumor have been accepted for organ donation because these tumors very rarely spread outside the CNS. However several case reports of CNS tumor transferral with organ transplantation recently challenged this attitude. Some risk factors for extraneural spread of CNS tumors have been determined, but the absence of risk factors does not exclude the possibility of metastases. To our knowledge, 13 cases of CNS tumor transferral with organ transplantation (one heart, three livers, eight kidneys, one kidney/pancreas) have been reported in the literature. Even if no prospective evaluation of the CNS tumor transmission risk with transplantation has been undergone, this risk may be estimated between a little more than 0% and 3% from retrospective series. The authors consider that patients with CNS tumor should be accepted as donors as long as the risk of dying on the waiting lists is significantly higher than the tumor transferral risk. Therefore the authors would have no restriction for transplanting organs from donors with benign or low-grade CNS tumor. For high-grade tumors, the authors would consider these donors as "marginal donors," and balance the risk of tumor transmission with the medical condition of the recipient. [less ▲]

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See detailOrgan failure and tight glycemic control in the SPRINT study.
Chase, J Geoffrey; Pretty, Christopher G; Pfeifer, Leesa et al

in Critical Care (2010), 14(4), 154

INTRODUCTION: Intensive care unit mortality is strongly associated with organ failure rate and severity. The sequential organ failure assessment (SOFA) score is used to evaluate the impact of a successful ... [more ▼]

INTRODUCTION: Intensive care unit mortality is strongly associated with organ failure rate and severity. The sequential organ failure assessment (SOFA) score is used to evaluate the impact of a successful tight glycemic control (TGC) intervention (SPRINT) on organ failure, morbidity, and thus mortality. METHODS: A retrospective analysis of 371 patients (3,356 days) on SPRINT (August 2005 - April 2007) and 413 retrospective patients (3,211 days) from two years prior, matched by Acute Physiology and Chronic Health Evaluation (APACHE) III. SOFA is calculated daily for each patient. The effect of the SPRINT TGC intervention is assessed by comparing the percentage of patients with SOFA </=5 each day and its trends over time and cohort/group. Organ-failure free days (all SOFA components </=2) and number of organ failures (SOFA components >2) are also compared. Cumulative time in 4.0 to 7.0 mmol/L band (cTIB) was evaluated daily to link tightness and consistency of TGC (cTIB >/=0.5) to SOFA </=5 using conditional and joint probabilities. RESULTS: Admission and maximum SOFA scores were similar (P = 0.20; P = 0.76), with similar time to maximum (median: one day; IQR: 13 days; P = 0.99). Median length of stay was similar (4.1 days SPRINT and 3.8 days Pre-SPRINT; P = 0.94). The percentage of patients with SOFA </=5 is different over the first 14 days (P = 0.016), rising to approximately 75% for Pre-SPRINT and approximately 85% for SPRINT, with clear separation after two days. Organ-failure-free days were different (SPRINT = 41.6%; Pre-SPRINT = 36.5%; P < 0.0001) as were the percent of total possible organ failures (SPRINT = 16.0%; Pre-SPRINT = 19.0%; P < 0.0001). By Day 3 over 90% of SPRINT patients had cTIB >/=0.5 (37% Pre-SPRINT) reaching 100% by Day 7 (50% Pre-SPRINT). Conditional and joint probabilities indicate tighter, more consistent TGC under SPRINT (cTIB >/=0.5) increased the likelihood SOFA </=5. CONCLUSIONS: SPRINT TGC resolved organ failure faster, and for more patients, from similar admission and maximum SOFA scores, than conventional control. These reductions mirror the reduced mortality with SPRINT. The cTIB >/=0.5 metric provides a first benchmark linking TGC quality to organ failure. These results support other physiological and clinical results indicating the role tight, consistent TGC can play in reducing organ failure, morbidity and mortality, and should be validated on data from randomised trials. [less ▲]

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See detailOrgan Procurement After Euthanasia: Belgian Experience
Ysebaert, dirk; Van Beeumen, G.; De Greef, K. et al

in Transplantation Proceedings (2009), 41

Euthanasia was legalized in Belgium in 2002 for adults under strict conditions. The patient must be in a medically futile condition and of constant and unbearable physical or mental suffering that cannot ... [more ▼]

Euthanasia was legalized in Belgium in 2002 for adults under strict conditions. The patient must be in a medically futile condition and of constant and unbearable physical or mental suffering that cannot be alleviated, resulting from a serious and incurable disorder caused by illness or accident. Between 2005 and 2007, 4 patients (3 in Antwerp and 1 in Liège) expressed their will for organ donation after their request for euthanasia was granted. Patients were aged 43 to 50 years and had a debilitating neurologic disease, either after severe cerebrovascular accident or primary progressive multiple sclerosis. Ethical boards requested complete written scenario with informed consent of donor and relatives, clear separation between euthanasia and organ procurement procedure, and all procedures to be performed by senior staff members and nursing staff on a voluntary basis. The euthanasia procedure was performed by three independent physicians in the operating room. After clinical diagnosis of cardiac death, organ procurement was performed by femoral vessel cannulation or quick laparotomy. In 2 patients, the liver, both kidneys, and pancreatic islets (one case) were procured and transplanted; in the other 2 patients, there was additional lung procurement and transplantation. Transplant centers were informed of the nature of the case and the elements of organ procurement. There was primary function of all organs. The involved physicians and transplant teams had the well-discussed opinion that this strong request for organ donation after euthanasia could not be waived. A clear separation between the euthanasia request, the euthanasia procedure, and the organ procurement procedure is necessary. [less ▲]

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See detailOrgan-dependent oxylipin signature in leaves and roots of salinized tomato plants (Solanum lycopersicum)
Ghanem, Michel Edmond; Ghars, Mohamed ali; Frettinger, Patrick et al

in Journal of Plant Physiology (2012), 169

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See detailLes organes de gestion et de représentation des ASBL belges
Caprasse, Olivier ULg

in ASBL, Fondations et Associations internationales (2004)

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See detailLes organes des fondations
Caprasse, Olivier ULg

in ASBL, Fondations et Associations internationales (2004)

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See detailLes organes du système immunitaire
Heinen, Ernst ULg; Defresne, M. P.; Boniver, J. et al

in Immunologie Animale (1990)

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See detailOrganic Acids, Sugars, and Anthocyanins Contents in Juices of Tunisian Pomegranate Fruits
Hasnaoui, Nejib ULg; Jbir, Rania; Mars, Messaoud et al

in International Journal of Food Properties (2011), 14(4), 741-757

Juices made from fruits of 30 Tunisian accessions of pomegranate were studied for their organic acids, sugars, and anthocyanin contents, using high performance liquid chromatography. Among the detected ... [more ▼]

Juices made from fruits of 30 Tunisian accessions of pomegranate were studied for their organic acids, sugars, and anthocyanin contents, using high performance liquid chromatography. Among the detected organic acids, malic acid was the major one (>50%) followed by citric acid (>22%), while among sugars, fructose and glucose were most present in pomegranate juice contributing 53.9 and 43.4% of the total sugar content, respectively. The total anthocyanin content ranged from 9-115 mg per litre of juice with the following ranges of the six compounds found: cyanidin-3,5-diglucoside (3.1-74.4 mg/L), delphinidin-3-glucoside (0.7-22.0 mg/L), cyanidin-3-glucoside (0.8-21.0 mg/L), pelargonidin-3-glucoside (0.5-16.1 mg/L), pelargonidin-3,5-diglucoside (0.0-11.8 mg/L), and delphinidin-3,5-diglucoside (0.0-5.4 mg/L). Based on the analyzed parameters, cluster analysis allowed grouping cultivars into two main clusters. One was made of sour cultivars and the second of the sweet ones. Principle component and cluster analyses suggested that the composition of the pomegranate fruits is determined by cultivar rather than cultivation location. [less ▲]

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See detailOrganic and mineral imprints in fossil photosynthetic mats of an East Antarctic lake
Lepot, Kevin; Compère, Philippe ULg; Gerard, E et al

in Geobiology (2014), 12(5), 424-450

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See detailOrganic C, N, del 13C and del 15N of suspended particulate matter in the Biscay Shelf Break area
Dauby, Patrick ULg; Frankignoulle, Michel; Elskens, Marc

Conference (1995, February)

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See detailOrganic carbon metabolism and carbonate dynamics in a Mediterranean seagrass (Posidonia oceanica) meadow
Barrón, Cristina; Duarte, Carlos M.; Frankignoulle, Michel et al

in Estuaries and Coasts (2006), 29(3), 417-426

in a Posidonia oceanica meadow and unvegetated sediments of Magalluf Bay (Mallorca Island, Spain) to determine gross primary production (GPP), community respiration (R), and net community production (NCP ... [more ▼]

in a Posidonia oceanica meadow and unvegetated sediments of Magalluf Bay (Mallorca Island, Spain) to determine gross primary production (GPP), community respiration (R), and net community production (NCP). From June 2001 to October 2002, we also measured fluxes of dissolved inorganic carbon (DIC) and total alkalinity (TAlk). The yearly integrated metabolic rates based on DO changes show that the P. oceanica communities are net autotrophic while the metabolic rates in the unvegetated benthic communities are nearly balanced. Higher calcium carbonate (CaCO3) cycling, both in terms of production and dissolution, was observed in P. oceanica communities than in unvegetated benthic communities. In the P. oceanica meadow, the annual release of CO2 from net CaCO3 production corresponds to almost half of the CO2 uptake by NCP based on DIC incubations. In unvegetated benthic communities, the annual uptake of CO2 from net CaCO3 dissolution almost fully compensates the CO2 release by NCP based on DIC incubations. CaCO3 dynamics is potentially a major factor in CO2 benthic fluxes in seagrass and carbonate-rich temperate coastal ecosystems. [less ▲]

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