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Peer Reviewed
See detailMolecular dissection of the Bovine Leukemia Virus external envelope glycoprotein using synthetic peptides.
Callebaut, Isabelle; Burny, Arsène; Krchnak, Viktor et al

in AIDS Research and Human Retroviruses (1992), 8

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See detailMolecular dissection of the color-sided phenotype in cattle reveals a novel mechanism of chromosome evolution involving circular shuttling intermediates.
Durkin, Keith ULg; Cambisano, Nadine ULg; Ahariz, Naïma ULg et al

in Chromosome Research : An International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology (2011, May), 19(S1), 18

The color-sided (Cs) phenotype is a dominant coat color pattern segregating in several breeds including Belgian Blue Cattle (BBC) and Brown Swiss (BS). A genome-wide association study performed in BBC ... [more ▼]

The color-sided (Cs) phenotype is a dominant coat color pattern segregating in several breeds including Belgian Blue Cattle (BBC) and Brown Swiss (BS). A genome-wide association study performed in BBC unambiguously positioned the Cs locus on chromo- some 29 (BTA29); however, SNP arrays and CGH detected an equally perfectly associated <480 kb duplication encompassing the KIT gene on chromo- some 6 (BTA6). FISH analysis reconciled these results by revealing an intrachromosomal duplication, which transposed a fragment of BTA6 to BTA29. The organization of the duplicated segment, including breakpoint definition, was determined by high-throughput resequencing and revealed that the transpo- sition occurred via a circular intermediate. The trans- posed KIT copy was shown to be transcriptionally competent, suggesting that dominant color-sidedness results from dysregulated expression of KIT. Similar analyses of the color-sided phenotype conducted in BS revealed linkage on BTA6, a <120- kb-BTA6 duplication (which overlaps with the BBC duplication), and a <414-kb-BTA29 duplication adja- cent to the BTA29 breakpoint defined in BBC. FISH analysis showed the duplicated portion of BTA29 was located on BTA6 and adjacent to the KIT gene. SNP genotyping indicated that the BTA6 and BTA29 haplotypes associated with color-sidedness in BS and BBC were near identical, demonstrating the non-independence of the two chromosomal events. High-throughput resequencing of a color-sided BS animal defined the corresponding breakpoints and suggests that the BS Cs allele is derived from the BBC duplication [less ▲]

Detailed reference viewed: 75 (19 ULg)
Peer Reviewed
See detailMolecular dissection of the insulin-like growth factor axis in the human thymus
Geenen, Vincent ULg; Kecha, Ouafae; Achour, Imane et al

in Diabetologia (1997), 40

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See detailMolecular Diversity of Antarctic Cyanobacteria
De Carvalho Maalouf, Pedro ULg; Zakhia, Frédéric; Chflami, Souad et al

Scientific conference (2008, December 12)

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See detailMolecular diversity of Microcystis strains (Cyanophyceae, Chroococcales) based on 16S rDNA sequences
LEPERE, Claire ULg; Wilmotte, Annick ULg; Meyer, Barbara

in Systematics and Geography of Plants (2000), 70

Partial 16S rDNA sequences were determined for six Microcystis strains from lakes in the region of Plön (Northern Germany) assigned to four different species on the basis of their morphology. These ... [more ▼]

Partial 16S rDNA sequences were determined for six Microcystis strains from lakes in the region of Plön (Northern Germany) assigned to four different species on the basis of their morphology. These sequences appear very similar to each other and to those of 71 Microcystis strains from four continents available in the databases. This great genotypic homogeneity, as measured by 16S rDNA sequence similarity (334 characters), is contrasting with the conspicuous morphological differences observed for the studied strains. [less ▲]

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See detailMolecular diversity of microorganisms in Antarctic lacustrine microbial mats
Wilmotte, Annick ULg; Stelmach Pessi, Igor ULg; Sweetlove, Maxime et al

Conference (2015, February)

The BeSPO project CCAMBIO aims to study the biogeographical distribution of microorganisms in lacustrine microbial mats using a combination of techniques including microscopical observations (light and ... [more ▼]

The BeSPO project CCAMBIO aims to study the biogeographical distribution of microorganisms in lacustrine microbial mats using a combination of techniques including microscopical observations (light and electronic), strain isolation, and molecular diversity assessment using Next Generation Sequencing. The samples were collected in different Antarctic and sub-Antarctic biogeographical regions. A multivariate analysis of diatoms showed that these regions hosted different diatom flora. Endemic diatom taxa were also observed, and a multigene molecular phylogeny of Pinnularia borealis showed a high genetic diversity. A new Scenedesmacean species was described from Antarctica, Chodatodesmus australis. A comparison of the bacterial diversity retrieved by cultivation or NGS showed a complementarity of both approaches and differences when different variable regions of the 16S rRNA gene were used. Novel and unclassified sequences, also observed by other authors, were obtained. Pilot studies were conducted for the microeukaryotes and cyanobacteria to select NGS protocols and bioinformatic pipelines. The purpose is to deposit the diversity data in the “Microbial Antarctic Resource System (MARS)” presently developed into the webportal ‘biodiversity.aq’. [less ▲]

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See detailMolecular dynamic simulation of the cyclic lipodepsipeptide Pseudodesmin A self-assembly
Crowet, Jean-Marc ULg; Sinnaeve, Davy; Fehér, Krisztina et al

Conference (2014, February 10)

Pseudodesmine A is a cyclic lipodepsipeptide of nine residues which presents a moderate antibacterial activity and whose structure has been resolved by X-ray and NMR1,2. In acetonitrile, Pseudodesmine A ... [more ▼]

Pseudodesmine A is a cyclic lipodepsipeptide of nine residues which presents a moderate antibacterial activity and whose structure has been resolved by X-ray and NMR1,2. In acetonitrile, Pseudodesmine A is monomeric while in chloroform it has the same structure but assemble in a supramolecular complex. This structure could associate with membranes and be responsible of the biological activity of this peptide. Comparison of the NMR data between the two solvents has given indications on the intermolecular contacts that arise in chloroform and a model for the self association was proposed2,3. To study in more details this assembly, molecular dynamics have been carried on. In acetonitrile, the peptide show transient interactions while in chlorofom interactions between monomers was always observed. As stated in Sinnaeve et al. in 2009, these interactions arise mainly between the backbone protons of the LEU1 and the GLN2, the GLN2 sidechain and the loop located on the opposite end of the monomer structure. From 10 simulations of dimerization, hydrogen bonds were followed and specific interaction patterns were identified regarding the hydrogen bonds formed. The peptide interactions are mainly described by 13 interaction patterns; 8 with the peptides in a linear configuration, 1 perpendicular and 4 with peptides side by side. The patterns are characterized by 2 to 4 hydrogen bonds. From the linear dimer, it is possible to reconstruct filaments and, by combining a linear and a lateral dimer, it is possible to build fibrils with multi filaments, as expected in the NMR derived model. Besides, the perpendicular dimer can gives peptide rings that can also explain the potential ability of this peptide to form ion pores in membranes. 1. Sinnaeve, D., Michaux, C., Van hemel, J., Vandenkerckhove, J., Peys, E., Borremans, F. a. M., Sas, B., Wouters, J. and Martins, J. C. Tetrahedron 2009, 65, 4173–4181. 2. Sinnaeve, D., Hendrickx, P. M. S., Van Hemel, J., Peys, E., Kieffer, B. and Martins, J. C. Chemistry (Weinheim an der Bergstrasse, Germany) 2009, 15, 12653–62. 3. Sinnaeve, D., Delsuc, M.-A., Martins, J. C. and Kieffer, B. Chemical Science 2012, 3, 1284. [less ▲]

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See detailMolecular dynamic simulations of a beta amphiphilic peptide
Crowet, Jean-Marc ULg; Deschamps, Antoine; Soumillion, Patrice et al

Conference (2013, October 03)

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See detailMolecular dynamics simulations of laser-induced damage of nanostructures and solids.
Diakhate, Momar ULg

in Applied Physics A : Materials Science & Processing (2009), 96

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See detailMolecular effectors and modulators of hypericin-mediated cell death in bladder cancer cells
Buytaert, E.; Matroule, J. Y.; Durinck, S. et al

in Oncogene (2008)

Photodynamic therapy (PDT) is an anticancer approach utilizing a light-absorbing molecule and visible light irradiation to generate, in the presence of O(2), cytotoxic reactive oxygen species, which cause ... [more ▼]

Photodynamic therapy (PDT) is an anticancer approach utilizing a light-absorbing molecule and visible light irradiation to generate, in the presence of O(2), cytotoxic reactive oxygen species, which cause tumor ablation. Given that the photosensitizer hypericin is under consideration for PDT treatment of bladder cancer we used oligonucleotide microarrays in the T24 bladder cancer cell line to identify differentially expressed genes with therapeutic potential. This study reveals that the expression of several genes involved in various metabolic processes, stress-induced cell death, autophagy, proliferation, inflammation and carcinogenesis is strongly affected by PDT and pinpoints the coordinated induction of a cluster of genes involved in the unfolded protein response pathway after endoplasmic reticulum stress and in antioxidant response. Analysis of PDT-treated cells after p38(MAPK) inhibition or silencing unraveled that the induction of an important subset of differentially expressed genes regulating growth and invasion, as well as adaptive mechanisms against oxidative stress, is governed by this stress-activated kinase. Moreover, p38(MAPK) inhibition blocked autonomous regrowth and migration of cancer cells escaping PDT-induced cell death. This analysis identifies new molecular effectors of the cancer cell response to PDT opening attractive avenues to improve the therapeutic efficacy of hypericin-based PDT of bladder cancer. [less ▲]

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See detailMolecular Elasticity of a Mechanically-Linked Polymer
Lussis, Perrine ULg; Fustin, C.-A; Van Quaethem, A et al

Conference (2010, July 11)

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See detailMolecular epidemiology of canine parvovirus, Europe
Decaro, Nicolas; Desario, Costantino; Addie, Diane D. et al

in Emerging Infectious Diseases (2007), 13(8), 1222-1224

Canine parvovirus (CPV), which causes hemorrhagic enteritis in dogs, has 3 antigenic variants: types 2a, 2b, and 2c. Molecular method assessment of the distribution of the CPV variants in Europe showed ... [more ▼]

Canine parvovirus (CPV), which causes hemorrhagic enteritis in dogs, has 3 antigenic variants: types 2a, 2b, and 2c. Molecular method assessment of the distribution of the CPV variants in Europe showed that the new variant CPV-2c is widespread in Europe and that the viruses are distributed in different countries. [less ▲]

Detailed reference viewed: 47 (1 ULg)
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See detailMolecular epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae colonizing the digestive tract of patients admitted to intensive care units in a Belgian university hospital
CHRISTIAENS, Geneviève ULg; Damas, Pierre ULg; Docquier, J. D. et al

in American Society of Microbiology (Ed.) Program and Abstracts of the 46th Intersciences Conference on Antimicrobial Agents and Chemotherapy (2006, September)

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See detailMolecular epidemiology of norovirus in symptomatic and asymptomatic population in Burkina Faso
HUYNEN, Pascale ULg; Mauroy, Axel ULg; Martin, Caroline et al

Poster (2012, September)

Background Noroviruses (NoV), belonging to the family Caliciviridae, are now recognized as the leading cause of gastroenteritis outbreaks worldwide, and represent an important cause of sporadic ... [more ▼]

Background Noroviruses (NoV), belonging to the family Caliciviridae, are now recognized as the leading cause of gastroenteritis outbreaks worldwide, and represent an important cause of sporadic gastroenteritis in both children and adults. Many studies describe NoV epidemiology. However, few data are available about the NoV strains circulating in most of African countries, in particular in Burkina Faso. The population of Burkina Faso is characterized by the young age of its habitants, and most are living in rural environment. Objectives The purpose of this epidemiological study was to determine the prevalence of NoV in Bobo Dioulasso (Southern part of Burkina Faso) by molecular diagnosis methods in patients presenting or not gastroenteritis symptoms, to quantify the excreted viral load, and to genotype the circulating strains. Methods Patients with and without gastro-intestinal disorders were selected in several Health Care Centres of Bobo Dioulasso. Clinical and epidemiological data, as well as stool samples, were collected during 8 weeks through March to April 2011. Viral genomic RNA was automatically extracted with a Maxwell® (Promega) instrument. Molecular detection of genogroups (G) I, II and IV NoV in stool samples was performed by a home-made real-time RT-PCR targeting the ORF1-ORF2 polymerase junction region. For each positive sample, viral load was estimated by using standard curves (successive dilutions of recombinant GI and GII plasmids). Molecular characterization was performed on the detected strains, using both polymerase and capsid regions. Results NoV were detected in 21.6% of the 453 collected stool samples, with a distribution of 21.0% and 23.1% in the samples from the 319 symptomatic (SP) and the 134 asymptomatic patients (AP) respectively. Genogroup distribution was 7.2% for GI, 10.7% for GII and 3.1% for both GI and GII among SP’s samples, and was 11.2% for GI, 10.4% for GII and 1.5% for both GI and GII among AP’s samples. Average viral load values were higher for GI NoV in SP than in AP (p=0.02), when they were higher for GII NoV in AP than in SP (p=0.04). Phylogenic analysis showed a high degree of genotypical diversity in both groups of patients. One recombinant strain GII.7/GII.6 was also detected, to our knowledge, for the first time. Conclusion Even if a true pathogenic role of NoV could not be showed from the study design, it allowed to precise the molecular epidemiology of NoV strains prevalent in a representative country of the East African region. It also showed that asymptomatic patients could play an important role as a NoV “reservoir”. Despite the fact that GII strains, and more precisely those belonging to GII.4 genotype, are nowadays highly reported worldwide, the surprising proportion of NoV GI detected in this study suggests that GI and GII strains should be excreted in equal proportion in the environment. The origin of this epidemiologic difference, even if partially explained by the difference in immunity and genetic sensitivity of the population, is still to be solved. [less ▲]

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See detailMolecular epidemiology of norovirus infections in symptomatic and and asymptomatic children from Bobo Dioulasso, Burkina Faso
HUYNEN, Pascale ULg; Mauroy, Axel ULg; Martin, Caroline et al

in Journal of Clinical Virology (2013), 58

Background: Noroviruses (NoV) are a leading cause of gastroenteritis worldwide. Few epidemiologicaldata regarding the NoV strains circulating in African countries are available.Objectives: To determine ... [more ▼]

Background: Noroviruses (NoV) are a leading cause of gastroenteritis worldwide. Few epidemiologicaldata regarding the NoV strains circulating in African countries are available.Objectives: To determine the prevalence of NoV in Bobo Dioulasso (Burkina Faso) in both symptomaticand asymptomatic gastroenteritis patients.Study design: Patients both with and without gastro-intestinal disorders were selected. Clinical andepidemiological data, as well as stool samples, were collected through March to April 2011.NoV molecular detection (genogrouping and genotyping) and viral load quantification were also per-formed for all samples.Results: NoV were detected in 22.2% of the 418 collected stool samples (21.2% and 24.8% from the 293symptomatic patients (SP) and the 125 asymptomatic patients (ASP) respectively).Genogroup (G) distribution was 7.5%, 10.2% and 3.4% for GI, GII and both GI/GII respectively among SPand 12.0%, 11.2% and 1.6% for GI, GII and both GI/GII, respectively, among ASP.Average viral load values were higher in SP than in ASP for GI (p = 0.03) but not for GII.Phylogenic analysis showed a high degree of genotype diversity in SP and ASP. One recombinantGII.7/GII.6 sequence was, to the best of our knowledge, detected for the first time.Conclusions: This study enabled identification of the specific molecular epidemiology of NoV strains cir-culating in a representative country in Eastern Africa, and additionally showed that ASP could play animportant “reservoir” role. A high strain diversity was detected with a surprisingly high proportion ofNoV GI compared to the common genotypes usually reported in comparable epidemiological studies. [less ▲]

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See detailMolecular Evidence For An Andean Origin And A Secondary Gene Pool For The Lima Bean (Phaseolus Lunatus L..) Using Chloroplast Dna
Fofana, B.; Baudoin, Jean-Pierre ULg; Vekemans, X. et al

in Theoretical and Applied Genetics (1999), 98(2),

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See detailMolecular evidence of Anaplasma phagocytophilum in wild boar (Sus scrofa) in Belgium
Nahayo, Adrien; Bardiau, Marjorie ULg; Volpe, Rosario ULg et al

in BMC Veterinary Research (2014), 10(80),

Anaplasma phagocytophilum is a tick-borne pathogen of veterinary and human importance. Both ticks as vectors and vertebrates as reservoir hosts are essential for the cycle maintenance of this bacterium ... [more ▼]

Anaplasma phagocytophilum is a tick-borne pathogen of veterinary and human importance. Both ticks as vectors and vertebrates as reservoir hosts are essential for the cycle maintenance of this bacterium. Currently, the whole range of animal species reservoirs for A. phagocytophilum in natural environment is still unknown. Therefore, the aim of this study was to estimate the prevalence of infection with A. phagocytophilum in the wild boar population in southern Belgium. [less ▲]

Detailed reference viewed: 40 (7 ULg)