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See detailPregnancy induced hypertension. Principles of management
Brichant, Jean-François ULg

Conference (2004, June)

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See detailPregnancy patterns during the early fetal period in high producing dairy cows treated with GnRH or progesterone.
Bech-Sabat, G.; Lopez-Gatius, F.; Garcia-Ispierto, I. et al

in Theriogenology (2009), 71

In order to explore pregnancy patterns in high producing dairy cows treated with GnRH or progesterone at pregnancy diagnosis (Days 28-34), two consecutive experiments were designed. In Experiment 1, cows ... [more ▼]

In order to explore pregnancy patterns in high producing dairy cows treated with GnRH or progesterone at pregnancy diagnosis (Days 28-34), two consecutive experiments were designed. In Experiment 1, cows bearing a single embryo were randomly assigned to a PRID (n=40; cows fitted with a progesterone releasing intra-vaginal device for 28 days), GnRH (n=40; cows receiving GnRH) or Control (n=26; untreated cows) group. PRID treatment led to a rise in plasma progesterone concentrations in the 7 days following the onset of treatment compared to the other two groups. In Experiment 2, in which we also examined twin pregnancies, animals were randomly assigned to PRID (n=312) or GnRH (n=294) treatment groups. Treatments were the same as described for Experiment 1. Logistic regression procedures revealed that in cows with a single corpus luteum, the probability of pregnancy loss between the first (Days 28-34) and second (Days 65-62) pregnancy diagnosis decreased by a factor of 0.51 in the PRID group compared to the GnRH group. However, in cows with two or more corpora lutea, PRID treatment increased the likelihood of pregnancy loss by a factor of three, compared to GnRH treatment. In cows carrying twins, the conceptus reduction rate was higher (P=0.02) for the GnRH (36%) than for the PRID (16.4%) group. Formation of a new corpus luteum was recorded in 17.7% of cows in the GnRH group. Our results indicate that compared to GnRH treatment, progesterone treatment given at pregnancy diagnosis in high producing dairy cows, reduced by a factor of 0.51 and increased by a factor of 3 the probability of pregnancy loss in cows with a single or with two or more corpora lutea, respectively, and reduced the conceptus reduction rate in cows carrying twins. The practical implications of our findings are that in herds with a high incidence of early fetal loss of a non-infectious nature, treatment at the time of pregnancy diagnosis with PRID in cows with one corpus luteum and with GnRH in cows with two or more corpora lutea should offer considerable benefits. [less ▲]

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See detailPregnancy-associated glycoprotein (PAG) concentrations during the post partum period in four non-pregnant cows.
Ayad, A.; Melo de Sousa, Noelita ULg; Bella, Amina ULg et al

in Proceedings of the The Life Science Summit (2007)

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See detailPregnancy-associated glycoprotein (PAG) pattern and pregnancy detection in Boer goats using an ELISA with different antisera
Shahin, M; Friedrich, M; Gauly, M et al

in Small Ruminant Research (2013), 113

Pregnancy-associated glycoproteins (PAGs) are macromolecules produced by placental tissue and released into the maternal circulation where they allow pregnancy diagnosis and follow-up. The present study ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) are macromolecules produced by placental tissue and released into the maternal circulation where they allow pregnancy diagnosis and follow-up. The present study addresses the question to what extent plasma PAG determination may serve as a means of early pregnancy detection in goats in a similar way it is practiced in cows, and whether an ovine or bovine PAG-ELISA may be utilized to this end. Blood samples were collected from eight pregnant pluriparous Boer goat does twice weekly during the first seven seeks and the last four weeks of pregnancy and weekly in-between and during four weeks following parturition. Plasma PAG concentrations (mean±SEM) were determined using a competitive enzyme-linked immunosorbent assay. Assays were conducted with polyclonal antisera raised in rabbits against purified preparations of caprine (AS#706), ovine (AS#780) and bovine PAG (AS#726). In the assay systems purified bovine PAG served as standard and tracer and goat anti-rabbit IgG served as coating antibody. With the antibody raised against caprine PAG (AS#706) a steep increase to a climax of 69±9 ng/ml on day 56 of pregnancy was followed by a gradual decline to 16±3 ng/ml at parturition and 0.3±0.07 ng/ml four weeks postpartum. The results achieved with the antiovine PAG (AS#780) showed close similarity, a maximum of 92±14 ng/ml being reached at 56 days of pregnancy. With anti-bovine PAG (AS#726), the PAG level increased to a maximum of 3.1±0.2 ng/ml on day 105 of pregnancy and fluctuated around 3 ng/ml until the end of pregnancy. The difference between pregnant and non-pregnant does reached a significant level 21 days after conception, one week earlier than with caprine and ovine antisera. [less ▲]

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See detailPregnancy-Associated Glycoprotein (PAG) profiles during pregnancy and postpartum and progesterone (P4) profiles during pregnancy in native goats from Northeast of Brazil.
Melo de Sousa, Noelita ULg; Garbayo, J. M.; Figueiredo, J. R. et al

in Arquivos - Faculdade de Veterinária, UFRGS (1997), 25(1 (Suppl)), 304-305

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See detailPregnancy-Associated Glycoprotein (PAG) profiles during the peri-implantation period in recipients carrying bovine somatic clones: preliminary results
Heyman, Y; Chavatte-Palmer, P; Melo de Sousa, Noelita ULg et al

in Journal of Reproduction & Fertility. Abstract Series (2004), 17(2), 168169

Bovine pregnancy-associated glycoproteins (bPAGs) are secreted by binucleate cells of the placenta and can be assayed in maternal plasma as indicators of pregnancy and markers for the functional status of ... [more ▼]

Bovine pregnancy-associated glycoproteins (bPAGs) are secreted by binucleate cells of the placenta and can be assayed in maternal plasma as indicators of pregnancy and markers for the functional status of the trophoblast. The concentrations of PAGs vary differentially during the peri-implantation period. Large offspring syndrome (LOS) and abnormal placentation have been associated in cloned fetuses with abnormally increased pregnancy-specific protein 60 (PSP60). The aim of this study was to investigate the evolution of plasma levels of PAGs measured by the use of three different RIA systems in heifers after transfer of somatic cloned embryos and to compare that to plasma levels of control recipients during early pregnancy to distinguish which ones vary differentially. Cloned embryos were derived from nuclear transfer from fibroblasts of an adult cell line to in vitro-matured bovine oocytes according to Vignon et al. (1998 Theriogenology 49, 392). Control embryos were obtained in vitro after IVF and cultured in serum-free medium up to the blastocyst stage. By Day 7, cloned (n = 29) and control embryos (n = 10) were transferred to synchronous recipient heifers of the Normande breed (one embryo per recipient). Blood samples were taken every 2–3 days between Days 25 and 50 of pregnancy from recipients that had a positive progesterone test on Day 21, and the plasma was stored frozen until assay. Pregnancy status was monitored by repeated ultrasound scanning from Day 35 to Day 90. Concentrations of PAGs were determined by validated RIA assays for the different forms (PAGI67, PAG55+62, PAG55+59) using 3 antisera (AS 497, AS 706, AS 708, respectively) (Perenyi et al. 2002 Reprod. Domest. Anim. 37, 100–104). profiles of only the recipients that were confirmed pregnant by scanning over Day 50 (n = 18 clones, n = 4 controls) were analyzed and compared. Concentrations of PAGs measured by AS 497 and AS 706 were significantly higher in clones than in control recipients during the second month of pregnancy, indicating that the placenta of clones secreted these forms in a different manner compared to controls. Moreover, we found that concentrations of PAGs as determined with the two same antisera were higher for recipients with a cloned fetus that developed to term compared to those which had fetal loss before 3 months. For instance, using the AS 706, respective values of PAGs at Day 45 were 17.64 ± 7.59 and 10.96 ± 6.38 ng/mL for pregnancies with cloned and control fetuses, respectively, (P < 0.05) and 21.29 ± 4.77 and 12.88 ± 8.90 ng/mL in pregnancies with cloned fetuses that developed normally to term or died before 3 months of age, respectively, (P < 0.05). We conclude that our assays using two antisera could be a predictive test for fetal loss in clone pregnancies. Until now, recipients carrying cloned embryos that develop LOS during late pregnancy could not be detected by conventional assays in maternal serum or by scanning during the period of 35–50 days. This study provides a new diagnostic tool to detect them. Investigations are in progress to check the localization of these different forms of PAG in placentomes removed from recipients carrying somatic clones. [less ▲]

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See detailPregnancy-associated glycoprotein (PAG) profiles during the peri-implantation period in recipients carrying bovine somatic clones: preliminary results
Heyman, Yvan; Chavatte-Palmer, P.; Melo de Sousa, Noelita ULg et al

in Reproduction, Fertility and Development (2005), 17(1-2), 168

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See detailPregnancy-Associated Glycoprotein 55 [Fragment] from Ovis aries placenta - Access number P83202
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2002)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 55 kDa [Fragment] from Capra hircus placenta - Accession number P80935
Garbayo, Juana Maria; Beckers, Jean-François ULg

E-print/Working paper (1998)

N-terminal microsequence obtained after purification and characterization of placental proteins in Capra hircus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 55h [Fragment] from Ovis aries placenta - Access number P83500
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 57 [Fragment] from Ovis aries placenta - Access number P83203
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2002)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 58a [Fragment] from Ovis aries placenta - Access number P83493
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 58a [Fragment] from Ovis aries placenta - Access number P83493
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2002)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 58c [Fragment] from Ovis aries placenta - Access number - P83495
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 59 [Fragment] from Ovis aries placenta - Access number P83444
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2002)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 59 kDa [Fragment] from Dama dama placenta - Accession number P80934
Garbayo, Juana Maria; Wattiez, Ruddy; Falmagne, Paul et al

E-print/Working paper (1998)

N-terminal microsequence obtained after purification and characterization of placental proteins in Capra hircus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 59g [Fragment] from Ovis aries placenta - Access number P83499
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 60f [Fragment] from Ovis aries placenta - Access number P83498
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 61b [Fragment] from Ovis aries placenta - Access number P83494
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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