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See detailLCA-GE-005- Rapport de validation - VR001_V01
Hubert, Cédric ULiege; Ziemons, Eric ULiege; Hubert, Philippe ULiege

Report (2011)

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See detailLCAT activation properties of apo A-I CNBr fragments and conversion of discoidal complexes into spherical particles.
Vanloo, B.; Taveirne, J.; Baert, J. et al

in Biochimica et Biophysica Acta (1992), 1128(2-3), 258-66

We studied the substrate properties of the phospholipid-cholesterol-apolipoprotein complexes generated with apo A-I, apo A-I-CNBr fragments, apo A-II and apo A-IV for cholesterol esterification by the ... [more ▼]

We studied the substrate properties of the phospholipid-cholesterol-apolipoprotein complexes generated with apo A-I, apo A-I-CNBr fragments, apo A-II and apo A-IV for cholesterol esterification by the enzyme lecithin-cholesterol acyltransferase (LCAT). The kinetic parameters determined with the different complexes as substrates, showed that the complexes containing apo A-I and apo A-IV were about 40-times more efficient than those generated with the apo A-I fragments. In this system, the substrates containing apo A-II had the lowest efficiency. In spite of the differences in the kinetic parameters observed with the various apolipoprotein-lipid complexes, the cholesterol inserted in the complexes was esterified for more than 90% after 24 h in all systems studied. Based upon the results of the kinetic experiments, we followed the transformation of the discoidal complexes into spherical particles, due to the formation of a cholesteryl esters core, in the presence of low-density lipoproteins as an external source of cholesterol. We observed the formation of spherical particles by electron microscopy, after incubation of the discoidal complexes with LCAT for 24 h. The average percentage of cholesteryl esters in the converted particles was around 60% of the total cholesterol, varying between 40% for the apo A-I-CNBr-1-DPPC-cholesterol complex and up to 86% for the apo A-I-DPPC-cholesterol complex. The secondary structure of protein in the complexes was not significantly modified. However, the phospholipid phase transition disappeared, together with the parallel orientation of the phospholipid acyl chains with the helical segments of the apolipoproteins, as the phospholipids are organized in a monolayer at the surface of the spheres. [less ▲]

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See detailThe LCC15-MB human breast cancer cell line expresses osteopontin and exhibits an invasive and metastatic phenotype
Sung, V.; Gilles, Christine ULiege; Murray, A. et al

in Experimental Cell Research (1998), 241(2), 273-84

We have characterized the LCC15-MB cell line which was recently derived from a breast carcinoma metastasis resected from the femur of a 29-year-old woman. LCC15-MB cells are vimentin (VIM) positive ... [more ▼]

We have characterized the LCC15-MB cell line which was recently derived from a breast carcinoma metastasis resected from the femur of a 29-year-old woman. LCC15-MB cells are vimentin (VIM) positive, exhibit a stellate morphology in routine cell culture, and form penetrating colonies when embedded in three-dimensional gels of Matrigel or fibrillar collagen. They show high levels of activity in the Boyden chamber chemomigration and chemoinvasion assays, and like other invasive human breast cancer (HBC) cell lines, LCC15-MB cells activate matrix-metalloproteinase-2 in response to treatment with concanavalin A. In addition, these cells are tumorigenic when implanted subcutaneously in nude mice and recolonize bone after arterial injection. Interestingly, both the primary lesion and the bone metastasis from which LCC15-MB were derived, as well as the resultant cell line, abundantly express the bone matrix protein osteopontin (OPN). OPN is also expressed by the highly metastatic MDA-MB-435 cells, but not other invasive or noninvasive HBC cell lines. Expression of OPN is retained in the subcutaneous xenograft and intraosseous metastases of LCC15-MB as detected by immunohistochemistry. Both VIM and OPN expression have been associated with breast cancer invasion and metastasis, and their expression by the LCC15-MB cell line is consistent with its derivation from a highly aggressive breast cancer. These cells provide a useful model for studying molecular mechanisms important for breast cancer metastasis to bone and, in particular, the implication(s) of OPN and VIM expression in this process. [less ▲]

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See detailLck dephosphorylation at Tyr-394 and inhibition of T cell antigen receptor signaling by Yersinia phosphatase YopH.
Alonso, Andres; Bottini, Nunzio; Bruckner, Shane et al

in Journal of Biological Chemistry (2004), 279(6), 4922-8

A key virulence factor for Yersinia pestis, the etiologic agent of plague, is the tyrosine phosphatase YopH, which the bacterium injects into host cells. We report that treatment of human T lymphocytes ... [more ▼]

A key virulence factor for Yersinia pestis, the etiologic agent of plague, is the tyrosine phosphatase YopH, which the bacterium injects into host cells. We report that treatment of human T lymphocytes with a recombinant membrane-permeable YopH resulted in severe reduction in intracellular tyrosine phosphorylation and inhibition of T cell activation. The primary signal transducer for the T cell antigen receptor, the Lck tyrosine kinase, was specifically precipitated by a substrate-trapping YopH mutant, and Lck was dephosphorylated at its positive regulatory site, Tyr-394, in cells containing active YopH. By turning off Lck, YopH blocks T cell antigen receptor signaling at its very first step, effectively preventing the development of a protective immune response against this lethal bacterium. [less ▲]

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See detailLC–SPE–NMR–MS analysis of Strychnos usambarensis fruits from Rwanda
Cao, Martine ULiege; Muganga, Raymond; Nistor, Iolanda ULiege et al

in Phytochemistry Letters (2012), 5(0), 170-173

The novelty of the present work lies in the characterization of akagerine and palicoside in Strychnos usambarensis fruits by a hyphenated analytical method combining high-performance liquid chromatography ... [more ▼]

The novelty of the present work lies in the characterization of akagerine and palicoside in Strychnos usambarensis fruits by a hyphenated analytical method combining high-performance liquid chromatography coupled with solid-phase extraction (SPE), mass spectrometry (HPLC–MS) and NMR spectroscopy. Akagerine was already known in S. usambarensis roots but palicoside is described for the first time in the species. [less ▲]

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See detailLD-based haplotype encoding scheme with iterative pruning principal component analysis (ipPCA) to retrieve population substructures
Chaichoompu, Kridsadakorn ULiege; Fouladi, Ramouna ULiege; Wangkumhang, Pongsakorn et al

Poster (2014, April 29)

Objective To identify and differentiate between subpopulations using a rich set of genetic markers, as using reduced sets of genetic markers for these purposes can become challenging, especially when ... [more ▼]

Objective To identify and differentiate between subpopulations using a rich set of genetic markers, as using reduced sets of genetic markers for these purposes can become challenging, especially when similar geographic regions are involved or when spurious patterns are likely to exist. Method Single Nucleotide Polymorphisms (SNPs) are commonly used to capture variations between populations and often genome-wide SNP data are pruned based on linkage disequilibrium (LD) patterns. Notably, haplotype composition and the pattern of LD between markers may vary between larger populations but may also play a role within more confined geographic regions. Indeed, knowledge about haplotypes in unrelated individuals can reveal useful information about genetic ancestry. Here, we use iterative pruning principal component analysis (ipPCA) [1] to identify and characterize subpopulations in an unsupervised way. As input data, either pruned genome-wide SNP data are used (using PLINK 1.9 with the "indep-pairwise" option, window size = 100k, r2 < 0.25) or multilocus haplotype information derived from the genome-wide SNP panel (using BEAGLE 3.3.2 to infer haplotype). These approaches are applied to real-life data from 992 Thai individuals [2]. Result Preliminary results indicate that ipPCA applied to pruned SNP data or ipPCA that explicitly uses multilocus information (haplotypes) give complementary information about population substructure for geographically confined populations such as the Thai samples in this study. Both methods address different aspects of population structure. Detailed simulation studies are needed to identify the optimal scenarios for haplotype-based ipPCA. Conclusion In this work, we propose to combine an LD-based haplotype encoding scheme with the ipPCA machinery to retrieve fine population substructures. Despite the complexities that are associated with haplotype inference, added value can be obtained when the LD structure between SNPs is exploited in the search for relevant population strata. References 1. Intarapanich, A., et al., Iterative pruning PCA improves resolution of highly structured populations. BMC Bioinformatics, 2009. 10: p. 382. 2. Wangkumhang, P., et al., Insight into the peopling of Mainland Southeast Asia from Thai population genetic structure. PLoS One, 2013. 8(11): p. e79522. [less ▲]

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See detailLDSO: A program to simulate pedigrees and molecular information under various evolutionary forces
Ytournel, F.; Teyssèdre, S.; Roldan, D. et al

in Journal of Animal Breeding & Genetics (2012), 129(5), 417-421

Simulations are a major tool to evaluate new statistical methods and optimize experimental designs in the genomic era. However, this can only be achieved when the simulations are close enough to reality ... [more ▼]

Simulations are a major tool to evaluate new statistical methods and optimize experimental designs in the genomic era. However, this can only be achieved when the simulations are close enough to reality, as well as diverse enough to be realistic. For mapping studies, it is thus critical to re-create as much as possible the forces generating linkage (mutation, random drift, changes in population sizes, selection and pedigree structure) and the mechanisms producing trait genetic architecture (additivity, dominance, epistasis). We present here a computer program (ldso) simulating these phenomena. Optional outputs provide statistics on the linkage disequilibrium (LD) structure and the identity by descent between chromosomal segments, facilitating further data analyses. Furthermore, ldso enables the simulation of genomic data in known pedigrees, which sticks as precisely as possible to recent population history and structures of the long-range LD, allowing optimization of fine-mapping strategies. © 2012 Blackwell Verlag GmbH. [less ▲]

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See detailLe _Syntactic Reference Corpus of Medieval French_: Structure, outils et exploitation
Mazziotta, Nicolas ULiege

in Dister, Anne; Longrée, Dominique; Purnelle, Gérald (Eds.) 11es Journées internationales d'Analyse statistique des Données Textuelles (JADT) (2012)

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See detailLe bail et la pratique notariale : récents développements
Kohl, Benoît ULiege

Scientific conference (2009, February)

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See detailLe balbuzard pêcheur: histoire d'une sauvegarde
de Bellefroid, Marie-des-Neiges; Berny, Philippe; Corallo, Christian et al

Book published by Catiche productions (2013)

Ce grand rapace ayant frôlé l'extinction, le balbuzard voit ses effectifs se reconstituer progressivement grâce à des actions de protection de ses habitats mais aussi un programme d'études scientifiques ... [more ▼]

Ce grand rapace ayant frôlé l'extinction, le balbuzard voit ses effectifs se reconstituer progressivement grâce à des actions de protection de ses habitats mais aussi un programme d'études scientifiques: baguage, suivi des couples nicheurs, alimentation... [less ▲]

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See detailLe Bio entre qualité spécifique et qualité différenciée
Stassart, Pierre M ULiege

Conference (2002)

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See detailLe bloc officiel et résidentiel: phase 1
Van der Stede, Véronique ULiege

in Lebeau, Marc; Suleiman, Antoine (Eds.) Tell Beydar, Five Seasons of Excavations (1995-1999), A Preliminary Report (2003)

 Cet article est consacré au palais mis au jour à Tell Beydar, une cité-état du 3e millénaire av. n. è. localisée dans le nord-est de la Syrie. Le lecteur y trouvera une description détaillée du bâtiment ... [more ▼]

 Cet article est consacré au palais mis au jour à Tell Beydar, une cité-état du 3e millénaire av. n. è. localisée dans le nord-est de la Syrie. Le lecteur y trouvera une description détaillée du bâtiment, une analyse du système de circulation ainsi qu’une évaluation des possibilités d’éclairage de l’édifice et de celles de restitution d’un étage. Il se termine par une analyse fonctionnelle des pièces et replace le bâtiment dans le contexte de l’architecture palatiale du Bronze ancien. [less ▲]

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See detailLe Brésil et ses ambitions séculaires au statut de puissance
Santander, Sébastian ULiege

Scientific conference (2010, April 30)

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See detailLe caillou de Spinoza (à propos de Médias et Censure)
Durand, Pascal ULiege

Article for general public (2004)

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See detailLe calcaire
Gerrienne, Philippe ULiege

in Environnement (1988), 5/88

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See detailLe calcul des adsorbeurs pour le traitement des eaux usées par les méthodes du génie des réacteurs chimiques
Crine, Michel ULiege; Degée, Albert; Pirard, Jean-Paul ULiege

in Tribune de l'Eau (La) (1976), 29

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See detailLe Calendrier et son Histoire
Biémont, Emile ULiege

in Bulletin de la Classe des Sciences. Académie Royale de Belgique (1996), 6e série(VII), 15-71

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See detailLe Cambro-Ordovicien du Massif de Stavelot et sa couverture de Dévonien inférieur
Lamens, J.; Steemans, Philippe ULiege; Vanguestaine, M.

Conference (1988)

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See detailLe cancer de la prostate
GERARD, Xavier ULiege

Scientific conference (2011, November)

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See detailLe capital osseux : une épargne qui débute au berceau
Halkin, V; Reginster, Jean-Yves ULiege

in L'agenda Pédiatrie (2000), 22

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