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See detailMono- and binuclear Zn-beta-lactamase from Bacteroides fragilis: catalytic and structural roles of the zinc ions.
Paul-Soto, R.; Hernandez-Valladares, M.; Galleni, Moreno ULg et al

in FEBS letters (1998), 438(1-2), 137-40

The Bacteroides fragilis Zn-beta-lactamase is active with a mono- and a binuclear zinc site. The apoenzyme produced by removal of both Zn ions does not recover full activity upon readdition of Zn2+ in ... [more ▼]

The Bacteroides fragilis Zn-beta-lactamase is active with a mono- and a binuclear zinc site. The apoenzyme produced by removal of both Zn ions does not recover full activity upon readdition of Zn2+ in contrast to an active mono-Zn form prepared at pH 6.0. Differences in k(cat) values observed are substrate-dependent implying distinct mechanisms for the mono- and binuclear species. The substrate profile of a Zn,Cd hybrid obtained by selective exchange of one zinc ion is different from that of the Zn2 enzyme with a remarkable 15-fold increased activity with cefoxitin as substrate. [less ▲]

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See detailMono- and binuclear Zn2+-beta-lactamase. Role of the conserved cysteine in the catalytic mechanism.
Paul-Soto, R.; Bauer, R.; Frère, Jean-Marie ULg et al

in The Journal of biological chemistry (1999), 274(19), 13242-9

When expressed by pathogenic bacteria, Zn2+-beta-lactamases induce resistance to most beta-lactam antibiotics. A possible strategy to fight these bacteria would be a combined therapy with non-toxic ... [more ▼]

When expressed by pathogenic bacteria, Zn2+-beta-lactamases induce resistance to most beta-lactam antibiotics. A possible strategy to fight these bacteria would be a combined therapy with non-toxic inhibitors of Zn2+-beta-lactamases together with standard antibiotics. For this purpose, it is important to verify that the inhibitor is effective under all clinical conditions. We have investigated the correlation between the number of zinc ions bound to the Zn2+-beta-lactamase from Bacillus cereus and hydrolysis of benzylpenicillin and nitrocefin for the wild type and a mutant where cysteine 168 is replaced by alanine. It is shown that both the mono-Zn2+ (mononuclear) and di-Zn2+ (binuclear) Zn2+-beta-lactamases are catalytically active but with different kinetic properties. The mono-Zn2+-beta-lactamase requires the conserved cysteine residue for hydrolysis of the beta-lactam ring in contrast to the binuclear enzyme where the cysteine residue is not essential. Substrate affinity is not significantly affected by the mutation for the mononuclear enzyme but is decreased for the binuclear enzyme. These results were derived from kinetic studies on two wild types and the mutant enzyme with benzylpenicillin and nitrocefin as substrates. Thus, targeting drug design to modify this residue might represent an efficient strategy, the more so if it also interferes with the formation of the binuclear enzyme. [less ▲]

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See detailMonoamine Oxidase Inhibitors and Anesthesia: An Updated Literature Review
Blom-Peters, L.; Lamy, Maurice ULg

in Acta Anaesthesiologica Belgica (1993), 44(2), 57-60

Safe anesthesia for patients taking MAOIs has been possible since the 1980s using judicious choice of agents. The introduction of a new class of MAOIs (RIMA) has increased the margin of safety for these ... [more ▼]

Safe anesthesia for patients taking MAOIs has been possible since the 1980s using judicious choice of agents. The introduction of a new class of MAOIs (RIMA) has increased the margin of safety for these anesthetics. These drugs allow selective and rapidly reversible inhibition of type A-MAO. The pharmacologic effects of this new class of drug as well as their potential interactions with agents used in anesthesia are reviewed. [less ▲]

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See detailMonocatenary, branched, double-headed and bolaform surface active carbohydrate esters via photochemical thiol-ene/-yne reactions
Boyère, Cédric ULg; Broze, Guy ULg; Blecker, Christophe ULg et al

in Carbohydrate Research (2013), 380

An original and versatile method for the synthesis of a range of novel mannose-based surfactants was developed via metal-free photo-induced thiol-ene/-yne “click” reactions. This light-mediated ... [more ▼]

An original and versatile method for the synthesis of a range of novel mannose-based surfactants was developed via metal-free photo-induced thiol-ene/-yne “click” reactions. This light-mediated hydrothiolation reaction involving a thiolated mannose was successfully applied to terminal and internal alkenes, dienes and alkynes, leading to monocatenary, branched, double-headed and bolaform amphiphilic carbohydrate esters,respectively. A surface activity study showed that these new compounds possess valuable properties and display specific behavior at the air-water interface. It also demonstrated the greater flexibility of the thioether moiety in the spacer of the surfactants produced via a thiol-ene reaction in comparison withthe triazole heterocyclic rings in similar glucose-based surfactants synthesized elsewhere by the alkyne-azide 1,3-dipolar addition. [less ▲]

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See detailUn monochrome poétique de Georges Fourest
Robert, Laurent ULg

in Romanitas : Lenguas y Literaturas Romances (2007), 2

Cet article propose une lecture du « Pseudo-sonnet que les amateurs de plaisanterie facile proclameront le plus beau du recueil » extrait de La Négresse blonde (1909) de Georges Fourest. La démarche de ... [more ▼]

Cet article propose une lecture du « Pseudo-sonnet que les amateurs de plaisanterie facile proclameront le plus beau du recueil » extrait de La Négresse blonde (1909) de Georges Fourest. La démarche de Fourest est mise en relation à la fois avec celle des peintures «monochroïdales » d’Alphonse Allais et avec celle du « sonnet en yx » de Stéphane Mallarmé. Elle est finalement perçue dans toute son ambiguïté, entre la mise en cause radicale de la poésie et la facétie fin de siècle. [less ▲]

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See detailMonoclonal antibodies against bovine growth hormone (bGH) enhance the biological response to GH by prolonging GH binding to its liver receptors.
Beauloye, V.; Muaku, S. M.; Portetelle, Daniel ULg et al

in Endocrinology (1995), 136(Suppl 1), 344

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See detailMonoclonal antibodies against bovine growth hormone (bGH) potentiate GH-induced liver IGF-I synthesis and enhance GH binding to hepatic somatogenic receptors
Massart, Serge; Maiter, Dominique; Adam, Emmanuelle et al

in 73° Annual Meeting of the Endocrine Society (1991)

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See detailMonoclonal antibodies against GARP/TGF-b1 complexes inhibit the immunosuppressive activity of human regulatory T cells in vivo
Cuende, Julia; Liénart, Stéphanie; Dedobbeleer, Olivier et al

in Science Translational Medicine (2015)

Regulatory T cells (Tregs) are essential to prevent autoimmunity, but excessive Treg function contributes to cancer progression by inhibiting antitumor immune responses. Tregs exert contact-dependent ... [more ▼]

Regulatory T cells (Tregs) are essential to prevent autoimmunity, but excessive Treg function contributes to cancer progression by inhibiting antitumor immune responses. Tregs exert contact-dependent inhibition of immune cells through the production of active transforming growth factor–b1 (TGF-b1). On the Treg cell surface, TGF-b1 is in an inactive form bound to membrane protein GARP and then activated by an unknown mechanism. We demonstrate that GARP is involved in this activation mechanism. Two anti-GARP monoclonal antibodies were generated that block the production of active TGF-b1 by human Tregs. These antibodies recognize a conformational epitope that requires amino acids GARP137–139 within GARP/TGF-b1 complexes. A variety of antibodies recognizing other GARP epitopes did not block active TGF-b1 production by Tregs. In a model of xenogeneic graft-versus-host disease in NSG mice, the blocking antibodies inhibited the immunosuppressive activity of human Tregs. These antibodies may serve as therapeutic tools to boost immune responses to infection or cancer via a mechanism of action distinct from that of currently available immunomodulatory antibodies. Used alone or in combination with tumor vaccines or antibodies targeting the CTLA4 or PD1/PD-L1 pathways, blocking anti-GARP antibodies may improve the efficiency of cancer immunotherapy. [less ▲]

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See detailMonoclonal antibodies againstbovine growth hormone (bGH) enhance the biological response to GH by prolonging GH binding to its liver receptors
Beauloye, V.; Muaku, S. M.; Portetelle, Daniel ULg et al

in 77° Annual Meeting of the Endocrine Society (1994)

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See detailMonoclonal antibodies to bovine insulin-like growth factor-binding protein -2 and -3: production and preliminary characterization
Portetelle, Daniel ULg; Massart, Serge; Sneyers, Myriam et al

in Hormone binding proteins: physiology and clinical implications symposium (1995)

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See detailMonoclonal Antibodies To Growth Hormone (Gh) Prolong Liver Gh Binding And Gh-Induced Igf-I/Igfbp-3 Synthesis
Beauloye, V.; Muaku, Sm.; Lause, P. et al

in American Journal of Physiology - Endocrinology and Metabolism (1999), 277(2),

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See detailMonoclonal antibodies to oxytocin: production and characterization
Burgeon, Emmanuel; Chapleur, Michèle; Schoenen, Jean ULg et al

in Journal of Neuroimmunology (1991), 31

To induce a good immune response to oxytocin (OT) we developed a two-step technique to conjugate OT to thyroglobulin (TG) using glutaraldehyde. We obtained 30 hybridomas recognizing OT-ovalbumin ... [more ▼]

To induce a good immune response to oxytocin (OT) we developed a two-step technique to conjugate OT to thyroglobulin (TG) using glutaraldehyde. We obtained 30 hybridomas recognizing OT-ovalbumin conjugates and 16 stable lines. Three monoclonal antibodies were selected for further characterization. One of them (O13) was found to be very specific for OT using three different techniques (EIA, RIA and ICC); it si directed to the C-terminal tripeptide. The other two probably recongize tyrosine-containing epitope(s) also shared by vasopressin and other related nonapeptides. [less ▲]

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See detailMonoclonal antibody from hybrid hybridoma: two specificities in one molecule
Rentier, Bernard ULg; Cloes, Jean-Michel

Conference (1988)

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See detailMonoclonal Antibody Production - The Report And Recommendations Of Ecvam Workshop 23
Marx, U.; Embleton, Mj.; Fischer, R. et al

in Alternatives to Laboratory Animals (1997), 25(2),

Detailed reference viewed: 39 (2 ULg)
See detailMonoclonal antibody to growth hormone the discovery of a new variant, human placental growth hormone
Hennen, Georges ULg; Frankenne, F.; Closset, J. et al

in Monoclonal antibodies : Basic principles, experimental and clinical applications in endocrinology (1985)

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See detailA Monoclonal Elisa for Bovine Viral Diarrhoea Pestivirus Antigen Detection in Persistently Infected Cattle
Mignon, Bernard ULg; Dubuisson, J.; Baranowski, E. et al

in Journal of Virological Methods (1991), 35(2), 177-188

Detection of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is crucial to controlling mucosal disease. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal ... [more ▼]

Detection of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is crucial to controlling mucosal disease. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies raised against the 48-kDa glycoprotein and the 120/80-kDa protein was developed for detecting antigens in leucocytes of 3 persistently BVDV-infected calves. The test is simple, sensitive and rapid. Moreover the same ELISA was able to recognise Belgian field isolates of BVDV. These results show that the test can be applied in the field. [less ▲]

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See detailMonoclonal-Antibodies To Bovine Growth-Hormone Potentiate Hormonal Activity In-Vivo By Enhancing Growth-Hormone Binding To Hepatic Somatogenic Receptors
Massart, Serge; Maiter, D.; Portetelle, Daniel ULg et al

in Journal of Endocrinology (1993), 139(3),

Detailed reference viewed: 12 (1 ULg)