Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2009, December 16) Detailed reference viewed: 6 (0 ULg) Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2008, January 31) Detailed reference viewed: 3 (1 ULg) Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2008, February 25) Detailed reference viewed: 4 (1 ULg) Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2007, December 13) Detailed reference viewed: 2 (0 ULg) Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2006, May 05) Detailed reference viewed: 2 (0 ULg) Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2005, September) Detailed reference viewed: 4 (0 ULg) Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2005, April 07) Detailed reference viewed: 2 (0 ULg) Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2002, December) Detailed reference viewed: 5 (0 ULg) Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2003, June 16) Detailed reference viewed: 1 (0 ULg) Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2003, March 11) Detailed reference viewed: 1 (0 ULg) Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2002, November) Detailed reference viewed: 1 (0 ULg) Le Secret des GéantsBeckers, Albert ![]() in Conférence sur invitation : Cotonou - Bénin, 02 au 04 décembre 2002 - Abstract book (2002) Detailed reference viewed: 3 (0 ULg) Le Secret des GéantsBeckers, Albert ![]() Scientific conference (2005, February 26) Detailed reference viewed: 1 (0 ULg) The Secret History of Belgian SurrealismDelville, Michel ![]() in Samizdat (2001) Detailed reference viewed: 24 (1 ULg) Le secret médical dans la législation socialeKefer, Fabienne ![]() in Revue de Droit Pénal et de Criminologie (1992) Detailed reference viewed: 15 (4 ULg) Le secret professionnel de l'avocat , Le secret professionnel : protection ou abus de droitJacobs, Ann ; ; et alin Le secret professionnel de l'avocat (1980) Detailed reference viewed: 68 (1 ULg) Secret professionnel des agents du fiscOrban, Lionel ![]() in Revue Générale du Contentieux Fiscal (2007), 6 Detailed reference viewed: 70 (0 ULg) Secret professionnel, confidentialité et juriste d'entreprise : la nouvelle donneThirion, Nicolas ; in Journal des Tribunaux (2001) Detailed reference viewed: 18 (0 ULg) Secreted dipeptidyl peptidases as potential virulence factors for Microsporum canis.; Baldo, Aline ; et alin FEMS Immunology & Medical Microbiology (2008), 54(3), 299-308 Dermatophytoses caused by Microsporum canis are frequently encountered in cats and dogs; they are highly contagious and readily transmissible to humans. In this study, two single genes, respectively ... [more ▼] Dermatophytoses caused by Microsporum canis are frequently encountered in cats and dogs; they are highly contagious and readily transmissible to humans. In this study, two single genes, respectively coding for dipeptidyl peptidases IV and V (DppIV and DppV), were isolated and characterized. Both proteins share homology with serine proteases of the S9 family, some of which display properties compatible with implication in pathogenic processes. Both genes are expressed in vivo in experimentally infected guinea-pigs and in naturally infected cats, and when the fungus is grown on extracellular matrix proteins as the sole nitrogen and carbon source. DppIV and V were produced as active recombinant proteases in the yeast Pichia pastoris; the apparent molecular weight of rDppV is 83 kDa, whereas rDppIV appears as a doublet of 95 and 98 kDa. Like other members of its enzymatic subfamily, rDppIV has an unusual ability to cleave Pro-X bonds. This activity does not enhance the solubilization of keratin by fungal secreted endoproteases, and the protease probably acts solely on small soluble peptides. RDppV showed no ability to induce delayed-type hypersensitivity (DTH) skin reactions in guinea-pigs, despite the known immunogenic properties of homologous proteins. [less ▲] Detailed reference viewed: 20 (3 ULg) Secreted metalloprotease gene family of Microsporum canis; ; et al in Infection and Immunity (2002), 70(10), 5676-5683 Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently ... [more ▼] Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently reported. Using Aspergillus fumigatus metalloprotease genomic sequence (MEP) as a probe, three genes (MEP1, MEP2, and MEP3) were isolated from an M. canis genomic library. They presented a quite-high percentage of identity with both A. fumigatus MEP and Aspergillus oryzae neutral protease I genes. At the amino acid level, they all contained an HEXXH consensus sequence, confirming that these M. canis genes (MEP genes) encode a zinc-containing metalloprotease gene family. Furthermore, MEP3 was found to be the gene encoding a previously isolated M. canis 43.5-kDa keratinolytic metalloprotease, and was successfully expressed as an active recombinant enzyme in Pichia pastoris. Reverse transcriptase nested PCR performed on total RNA extracted from the hair of M. canis-infected guinea pigs showed that at least MEP2 and MEP3 are produced during the infection process. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes. [less ▲] Detailed reference viewed: 27 (1 ULg) |
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