Secondary-structure characterization by far-UV CD of highly purified uncoupling protein 1 expressed in yeast

in Biochemical Journal (2004), 380(Pt 1), 139-145

The rat UCP1 (uncoupling protein 1) is a mitochondrial inner-membrane carrier involved in energy dissipation and heat production. We expressed UCP1 carrying a His(6) epitope at its C-terminus in ... [more ▼]

The rat UCP1 (uncoupling protein 1) is a mitochondrial inner-membrane carrier involved in energy dissipation and heat production. We expressed UCP1 carrying a His(6) epitope at its C-terminus in Saccharomyces cerevisiae mitochondria. The recombinant-tagged UCP1 was purified by immobilized metal-ion affinity chromatography to homogeneity (>95 %). This made it suitable for subsequent biophysical characterization. Fluorescence resonance energy transfer experiments showed that n-dodecyl-beta-D-maltoside-solubilized UCPI-His(6) retained its PN (purine nucleotide)-binding capacity. The far-UV CD spectrum of the functional protein clearly indicated the predominance of a-helices in the UCP1 secondary structure. The UCP1 secondary structure exhibited an alpha-helical degree of approx. 68 %, which is at least 25 % higher than the previously reported estimations based on computational predictions. Moreover, the helical content remained unchanged in free and PN-loaded UCP1. A homology model of the first repeat of UCP1, built on the basis of X-ray-solved close parent, the ADP/ATP carrier, strengthened the CD experimental results. Our experimental and computational results indicate that (i) alpha-helices are the major component of UCP1 secondary structure; (ii) PN-binding mechanism does not involve significant secondary-structure rearrangement; and (iii) UCP1 shares similar secondary-structure characteristics with the ADP/ATP carrier, at least for the first repeat. [less ▲]

Seconde campagne de mesure des odeurs sur le CET "Cour-au-Bois" à Braine-le-Château Estimation des nuisances olfactives

Report (2004)

The study is made in the frame of a follow-up monitoring of all landfill sites in Wallonia, initiated by the Ministry of Environment and managed by ISSeP. The present report concerns a second campaign on ... [more ▼]

The study is made in the frame of a follow-up monitoring of all landfill sites in Wallonia, initiated by the Ministry of Environment and managed by ISSeP. The present report concerns a second campaign on the landfill site "Cour-au-Bois" at Braine-le-Château. The usual field inspection technique was applied together with a GC-MS analysis aiming at determining the chemical composition of the ambiant air around the tipping area. [less ▲]

Seconde campagne de mesure des odeurs sur le CET de Happe- Chapois Estimation des nuisances olfactives

Report (2006)

The study is conducted in the frame of a programme of monitoring of all landfill sites in Wallonia. The "odour" part is assigned to ULg. The present report concerns more particularly a second campaign on ... [more ▼]

The study is conducted in the frame of a programme of monitoring of all landfill sites in Wallonia. The "odour" part is assigned to ULg. The present report concerns more particularly a second campaign on the site of Happe-Chapois. Besides the usual field inspection technique, the research group has used dynamic olfactometry to estimate the odour flux emitted by the tipping area, through a sample collected by a dynamic flux chamber. The report presents also a critical discussion about the advantages and limits of the field inspection technique. [less ▲]

La seconde génération des immigrés italiens à Herstal

Report (1985)

La seconde ondulation positive (première ondulation systolique) du pouls veineux physiologique chez le chien

in Archives Internationales de Physiologie (1907), V

Seconde réunion du projet de recherche du 7ème programme cadre de recherches de la Commission européenne

Scientific conference (2008, December 15)

Secondiare , Bac 1, une transition difficille. Pour quelles raisons?

Scientific conference (2009, December 04)

Le Secret des Géants - Mise au point des adénomes à TSH

Scientific conference (2002, September 20)

Le secret des géants

Scientific conference (2011, June 09)

Le Secret des Géants

Scientific conference (2008, January 31)