second breadboard design summary, preparation and manufacturing

Report (2007)

The second European evidence-based Consensus on the diagnosis and management of Crohn's disease: Definitions and diagnosis.

in Journal of Crohn’s and Colitis [=JCC] (2010), 4(1), 7-27

Second harmonic generation as a tool to probe the interactions of peptides with membrane-mimicking interfacial monolayers

Conference (2011)

Second harmonic generation to monitor the interactions of the antimicrobial mycosubtilin with membrane-mimicking interfacial monolayers

in BioNanoScience (2012), 2(2), 108-112

Mycosubtilin is a strong antimicrobial agent belonging to the iturinic lipopeptide family which contains a single tyrosine residue. Its cell target has been shown to be the cytoplasmic membrane. This ... [more ▼]

Mycosubtilin is a strong antimicrobial agent belonging to the iturinic lipopeptide family which contains a single tyrosine residue. Its cell target has been shown to be the cytoplasmic membrane. This tyrosine residue has been previously shown to be essential for the biological activity of mycosubtilin. Since we have previously demonstrated that tyrosine, an aromatic amino acid, can be used as an endogenous probe for the frequency doubling process, the presence of a tyrosine residue in mycosubtilin allowed us to investigate the interactions of mycosubtilin with biomimetic lipid monolayers at the air–water interface by second harmonic generation (SHG). Mycosubtilin was added underneath dipalmitoylphosphatidylcholine or cholesterol monolayers at the air–water interface and significant increases in the surface pressure were observed in both cases. This observation demonstrates that mycosubtilin interacts with these biomimetic membranes. A light polarization resolved analysis of the SHG signals recovered for these two systems was then performed and confirmed that those interactions between the tyrosine residue in mycosubtilin and the membranes could be monitored by SHG. Furthermore, the differences exhibited by the nonlinear optical measurements for different membranes showed that these interactions depend on the nature of the biomimetic membrane present at the air–water interface. [less ▲]

Second order pseudo-maximum likelihood estimation and conditional variance misspecification

Conference (1997, August 27)

In this paper, we study the behavior of second order pseudo-maximum likelihood estimators under conditional variance misspecification. We determine sufficient and essentially necessary conditions for such ... [more ▼]

In this paper, we study the behavior of second order pseudo-maximum likelihood estimators under conditional variance misspecification. We determine sufficient and essentially necessary conditions for such a estimator to be, regardless of the conditional variance (mis)specification, consistent for the mean parameters when the conditional mean is correctly specified. These conditions implie that, even if mean and variance parameters vary independently, standard PML2 estimators are generally not robust to conditional variance misspecification. Further, we outline sufficient and essentially necessary conditions for a second order pseudo-maximum likelihood estimator to be consistent for both mean and variance parameters when the conditional mean and the conditional variance are jointly correctly specified, and to remain consistent for the mean parameters when the conditional mean is correctly specified but the conditional variance is not jointly correctly specified. Finally, we provide limiting distribution results for this latter robust to conditional variance misspecification class of estimators under different assumptions regarding the degree of misspecification present in the model, show that its asymptotic covariance matrix is bounded and compare its similarities with QGPML1. [less ▲]

Le second résident néerlandais en Ardenne, un étranger parmi d‘autres

in Diry, Jean-Paul (Ed.) Les étrangers dans les campagnes, Actes du colloque franco-britanique de géographie rurale (2008)

The paper underlines the diversity of the motivations of second homes owners and replaces this diversity in the new ways of living the countryside. The analysis of the distribution of second homes in ... [more ▼]

The paper underlines the diversity of the motivations of second homes owners and replaces this diversity in the new ways of living the countryside. The analysis of the distribution of second homes in Belgium may be enlightening by a Multiple Regression Model combining the subjective quality of environment and the home prices. From this step, the research analyses the diversity of the strategies of localisation in Ardennes and quests for the diversity of appropriation. In place of the common idea of regional space consumption by the Dutch holidaymakers, the paper underlines the involvement of “strangers” in the local community and compares these involvements with those from the other inhabitants like commuters and crossborder workers. [less ▲]

Second supplement to the catalogue of observed periods of Ap stars

in Astronomy and Astrophysics. Supplement Series (1991), 87

Supplementary data on the periods of Ap stars with references are presented 20 new stars are introduced for which a periodic variability has been discovered since 1987 or it was known before but it was ... [more ▼]

Supplementary data on the periods of Ap stars with references are presented 20 new stars are introduced for which a periodic variability has been discovered since 1987 or it was known before but it was not reported in the previous issues of this catalogue. For many of the stars in the previous catalogues new determinations of the period are introduced. Recently attributed variable star names are also reported. [less ▲]

Second-generation octarellins: two new de novo (beta/alpha)8 polypeptides designed for investigating the influence of beta-residue packing on the alpha/beta-barrel structure stability

in Protein Engineering (1995), 8(3), 249-59

The sequence of octarellin I, the first de novo (beta/alpha)8 polypeptide, was revised according to several criteria, among others the symmetry of the sequence, beta-residue volume and hydrophobicity, and ... [more ▼]

The sequence of octarellin I, the first de novo (beta/alpha)8 polypeptide, was revised according to several criteria, among others the symmetry of the sequence, beta-residue volume and hydrophobicity, and charge distribution. These considerations and the overall conclusions drawn from the first design led to two new sequences, corresponding to octarellins II and III. Octarellin II retains perfect 8-fold symmetry. Octarellin III has the same sequence as octarellin II, except for the beta-strands which exhibit a 4-fold symmetry. The two proteins were produced in Escherichia coli. Infrared and CD spectral analyses of octarellins II and III reveal a high secondary structure content. Non-denaturing gel electrophoresis, molecular sieve chromatography and analytical ultracentrifugation suggest that both of these second-generation artificial polypeptides exist as a mixture of a monomer and a dimer form. Octarellins II and III are at least 10 times more soluble than octarellin I. Urea-induced unfolding followed by fluorescence emission suggests that the tryptophan residues, designed to be buried in the (beta/alpha)8, are indeed packed in the hydrophobic core of both proteins. However, octarellin III displays a higher stability towards urea denaturation, indicating that introducing 4-fold symmetry into the beta-barrel might be important for stability of the overall folding. [less ▲]

Second-order Plastic-hinge Analysis of 3D Steel Frames including Strain Hardening Effects

in Engineering Structures (2008), 30

Second-site suppressor mutations for the serine 202 to phenylalanine substitution within the interdomain loop of the tetracycline efflux protein Tet(C)

in Journal of Biological Chemistry (2003), 278