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See detailLarge-scale Analysis of the human genome: from DNA sequence analysis to the modeling of replication in higher eukaryotes
Arneodo, Alain; d'Aubenton-Carafa, Y.; Audit, B. et al

in Proceeding of the 14th EUSIPCO (2006)

We explore large-scale nucleotide compositional fluctuations along the human genome through the optics of the wavelet transform mi- croscope. Analysis of the TA and GC skews reveals the existence of ... [more ▼]

We explore large-scale nucleotide compositional fluctuations along the human genome through the optics of the wavelet transform mi- croscope. Analysis of the TA and GC skews reveals the existence of strand asymmetries associated to transcription and/or replication The investigation of 14854 intron-containing genes shows that both skews display a characteristic step-like profile exhibiting sharp tran- sitions between transcribed (finite bias) and non-transcribed (zero bias) regions. As we observe for 7 out of 9 origins of replica- tion experimentally identified so far, the (AT+GC) skew exhibits rather sharp upward jumps, with a linear decreasing profile in be- tween two successive jumps. We describe a multi-scale method- ology that allows us to predict 1012 replication origins in the 22 human autosomal chromosomes. We present a model of replica- tion with well-positioned replication origins and random termina- tion sites that accounts for the observed characteristic serrated skew profiles. We emphasize these putative replication initiation zones as regions where the chromatin fiber is likely to be more open so that DNA be easily accessible. In the crowded environment of the cell nucleus, these intrinsic decondensed structural defects actually predisposes the fiber to spontaneously form rosette-like structures that provide an attractive description of genome organization into replication foci that are observed in interphase mammalian nuclei. [less ▲]

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See detailLarge-Scale Clinical Expansion of Mesenchymal Stem Cells in the GMP-Compliant, Closed Automated Quantum® Cell Expansion System: Comparison with Expansion in Traditional T-Flasks
LECHANTEUR, Chantal ULg; Baila, Stefano; Janssen, Michel Etienne et al

in Journal of Stem Cell Research & Therapy (2014), 4(8), 1000222

Objectives: Significant advances have been achieved regarding the knowledge of the immunoregulatory properties of mesenchymal stem cells (MSC). We are currently involved in several clinical protocols ... [more ▼]

Objectives: Significant advances have been achieved regarding the knowledge of the immunoregulatory properties of mesenchymal stem cells (MSC). We are currently involved in several clinical protocols evaluating these properties in different settings including hematopoietic cells or solid organ transplantation, and severe or refractory autoimmune disorders. Considering the large number of ex-vivo expanded cells required for these clinical protocols (MSC dose varies from 1 to 4x10-6 MSC/kg patient per infusion), we evaluated the Quantum® device, a GMPcompliant, functionally closed, automated hollow fiber bioreactor system and compared it with our traditional clinical culture system in flasks. Methods: Primary and pre-enriched MSC expansions were simultaneously conducted in both culture systems and evaluated in terms of expansion rates and compliance with quality specifications and ISCT-release criteria. Due to practical considerations, most of the experiments conducted in the bioreactor (P1 and P2 expansions) used thawed MSC. These were compared with both fresh and thawed MSC expansions in flasks. Results: The Quantum® device reproducibly produced therapeutic MSC doses that fulfill ISCT-release criteria, are sterile, devoid of mycoplasma and endotoxin, have normal karyotypes and demonstrate immunosuppressive and differentiation capacities in vitro. Cells also grew faster in the bioreactor than in flasks during passage P1 (doubling time 40 compared to 56 hours in flasks) and P2 expansions but not during the primary expansion phase (P0). Seeding 20x10-6 thawed P2-preselected cells on the device allowed us to harvest 110-276x10-6 MSC after a 7 day expansion; seeding 50x10-6 cells resulted in 291-334x10-6 MSC harvested. Conclusion: In conclusion, the Quantum® device is an excellent system to produce a clinical dose of MSC but cost-effectiveness varies as a function of the manufacturing strategy in place. For our particular situation, the use of the Quantum device didn't result in a cost saving solution. [less ▲]

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See detailLarge-scale coherent orientations of quasar polarisation vectors: interpretation in terms of axion-like particles
Payez, Alexandre ULg; Hutsemekers, Damien ULg; Cudell, Jean-René ULg

in AIP Conference Proceedings (2010), 1274,

The observation of redshift-dependent coherent orientations of quasar polarisation vectors over cosmological distances in some regions of the sky is reviewed. Based on a good-quality sample of 355 ... [more ▼]

The observation of redshift-dependent coherent orientations of quasar polarisation vectors over cosmological distances in some regions of the sky is reviewed. Based on a good-quality sample of 355 measured quasars, this observation seems to infer the existence of a new effect acting on light propagation on such huge distances. A solution in terms of nearly massless axion-like particles has been proposed in the literature and its current status is discussed. [less ▲]

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See detailLarge-scale eradication of rabies using recombinant vaccinia-rabies vaccine
Brochier, Bernard; Kieny, M. P.; Costy, F. et al

in Nature (1991), 354(19/26 December), 520-521

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See detailLarge-scale fire test of unprotected cellular beam acting in membrane action
Vassart, Olivier; Bailey, C.G.; Hawes, M. et al

in Proceedings of the Institution of Civil Engineers: Structures and Buildings (2012), 165(7), 327334

This paper describes a full scale fire test performed recently on a composite floor for analysing the possibility of tensile membrane action to develop when the unprotected steel beams in the central part ... [more ▼]

This paper describes a full scale fire test performed recently on a composite floor for analysing the possibility of tensile membrane action to develop when the unprotected steel beams in the central part of the floor are made of cellular beams. The natural fire was created by a wood crib fire load of 700 MJ/m² and the 9 x 15 m floor survived the fire that peaked at 1000°C and lasted for about 90 minutes. Blind predictions of the air temperature development by the software OZone and of the structural behaviour by the software SAFIR which proved quite satisfactory are also described. [less ▲]

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See detailLarge-Scale Fire Test of Unprotected Cellular Beam Acting in Membrane Action
Vassart, Olivier; Bailey, C. G.; Hawes, M. et al

in Journal of Structural Fire Engineering (2011), 2(4), 259-268

This paper describes a full scale fire test performed the 27th of February 2010 on a composite floor for analysing the possibility of tensile membrane action to develop when the unprotected steel beams in ... [more ▼]

This paper describes a full scale fire test performed the 27th of February 2010 on a composite floor for analysing the possibility of tensile membrane action to develop when the unprotected steel beams in the central part of the floor are made of cellular beams. The natural fire was created by a wood crib fire load of 700 MJ/m2 and the 9 × 15 m floor survived the fire that peaked at 1000°C and lasted for 90 minutes. Blind predictions of the air temperature development by the software OZone and of the structural behaviour by the software SAFIR which proved quite satisfactory are also described. [less ▲]

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See detailLarge-scale fire test of unprotected cellular beam acting in membrane action
Vassart, Olivier; Bailey, C. G.; Hawes, M. et al

in Franssen, Jean-Marc; Kodur, Venkatesh (Eds.) Proceedings of the Sixth International Conference Structures in Fire (2010, June)

This paper describes a full scale fire test performed recently on a composite floor for analysing the possibility of tensile membrane action to develop when the unprotected steel beams in the central part ... [more ▼]

This paper describes a full scale fire test performed recently on a composite floor for analysing the possibility of tensile membrane action to develop when the unprotected steel beams in the central part of the floor are made of cellular beams. The natural fire was created by a wood crib fire load of 700 MJ/m² and the 9 x 15 m floor survived the fire that peaked at 1000°C and lasted for 90 minutes. Blind predictions of the air temperature development by the software Ozone and of the structural behaviour by the software SAFIR which proved quite satisfactory are also described. [less ▲]

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See detailLarge-scale flow and transport modelling for the management of groundwater bodies: the Geer case-study
Orban, Philippe ULg; Goderniaux, Pascal ULg; Brouyère, Serge ULg

in AquaTerra Final Conference. Programme & Proceedings (2009, March)

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See detailLarge-scale groundwater flow and transport modeling: Methodology and application to the Meuse Basin – Belgium
Orban, Philippe ULg; Brouyère, Serge ULg; Corbeanu, Horatiu et al

in Bringing Groundwater Quality Research to the Watershed Scale - Proceedings of GQ2004 (2005)

To meet the requirements of the EU Water Framework Directive, an integrated water management project named PIRENE was initiated by the Walloon Region of Belgium. A partner of this project, the ... [more ▼]

To meet the requirements of the EU Water Framework Directive, an integrated water management project named PIRENE was initiated by the Walloon Region of Belgium. A partner of this project, the Hydrogeology Group of the University of Liège, is in charge of the development of a physically based transient groundwater flow and transport model for the Walloon part of the Meuse Basin (approximately 17 000 km2). To face the problems associated with this large-scale model, a general approach has been developed that combines a hydrogeological database and GIS systems to manage data. Techniques of spatial discretization have been applied that optimize the number of unknowns. For modelling groundwater flow and transport on a large scale, a new numerical approach called the Hybrid Finite Element Mixing Cell (HFEMC) has been implemented in the 3-D simulator SUFT3D. [less ▲]

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See detailLarge-scale groundwater flow and transport modelling : Methodology and application to the Geer basin, Belgium
Orban, Philippe ULg; Dassargues, Alain ULg; Brouyère, Serge ULg

in Stauffer, Fr.; Dassargues, Alain (Eds.) Quantitative Geology from Multiple Sources: S10 Use of multiple sources in conditioning/calibrating groundwater flow and transport models (2006)

Efficient and sustainable management of water resources should be considered from an integrated way, at the basin scale. For that purpose, physically-based, spatially distributed modelling tools has ... [more ▼]

Efficient and sustainable management of water resources should be considered from an integrated way, at the basin scale. For that purpose, physically-based, spatially distributed modelling tools has turned to be very useful. If regional groundwater flow model have been developed for years now, large scale contaminant transport models are still almost inexistent. The objective of this extended abstract is to present briefly new concepts for large transport modelling, more particularly a modelling approach, the Hybrid Finite Element Mixing Cell (HFEMC) developed by the Hydrogeology Group of the University of Liège and implemented in the 3D simulator SUFT3D. First steps for the application to the Geer basin are also presented. [less ▲]

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See detailLarge-scale groundwater modelling in the Walloon part of the Meuse basin: challenges and first steps
Brouyère, Serge ULg; Orban, Philippe ULg

Conference (2006, December 14)

This presentation described the first steps in the development of large (groundwater body) scale groundwater flow and transport modelling in the Meuse district

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See detailLarge-scale identification of selenium metabolites by online size-exclusion-reversed phase liquid chromatography with combined inductively coupled plasma (ICP-MS) and electrospray ionization linear trap-Orbitrap mass spectrometry (ESI-MS(n))
Preudhomme, Hugues; Far, Johann ULg; Gil-Casal, Sandra et al

in Metallomics (2012), 4(5), 422-432

A method was developed for the comprehensive cartography of the selenium metabolites synthesized in the process of conversion of selenite [Se(IV)] into organic compounds and enrichment of yeast with ... [more ▼]

A method was developed for the comprehensive cartography of the selenium metabolites synthesized in the process of conversion of selenite [Se(IV)] into organic compounds and enrichment of yeast with selenium. The number of compounds detected was considerably increased (49) owing to the optimization of the fractionation procedure and the use of UPLC. The increased purity of the minor selenoorganic amino acids and oligopeptides allowed successful on-line de-novo identification based on the exact mass (o1 ppm) and fragmentation (MS2/MS3) mass spectra obtained with high resolution and high mass accuracy. The quality of mass spectra allowed the re-interpretation of previously reported structures of some selenium species. [less ▲]

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See detailLarge-scale modelling within the PIRENE programme in the Walloon Region of Belgium
Dassargues, Alain ULg

Scientific conference (2003, September 29)

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See detailA large-scale multicentre study of patient skin doses in interventional cardiology: dose-area product action levels and dose reference levels.
Bogaert, E.; Bacher, K.; Lemmens, K. et al

in The British journal of radiology (2009), 82(976), 303-12

For 318 patients in 8 different Belgian hospitals, the entire skin-dose distribution was mapped using a grid of 70 thermoluminescence dosimeters per patient, allowing an accurate determination of the ... [more ▼]

For 318 patients in 8 different Belgian hospitals, the entire skin-dose distribution was mapped using a grid of 70 thermoluminescence dosimeters per patient, allowing an accurate determination of the maximum skin dose (MSD). Dose-area product (DAP) values, exposure parameters and geometry, together with procedure, patient and cardiologist characteristics, were also registered. Procedures were divided into two groups: diagnostic procedures (coronary angiography) and therapeutic procedures (dilatation, stent, combined procedures (e.g. coronary angiography + dilatation + stent)). The mean value of the MSD was 0.310 Gy for diagnostic and 0.699 Gy for therapeutic procedures. The most critical projection for receiving the MSD is the LAO90 (left anterior oblique) geometry. In 3% of cases, the MSD exceeded the 2 Gy dose threshold for deterministic effects. Action levels in terms of DAP values as the basis for a strategy for follow-up of patients for deterministic radiation skin effects were derived from measured MSD and cumulative DAP values. Two DAP action levels are proposed. A first DAP action level of 125 Gy cm(2) corresponding to the dose threshold of 2 Gy would imply an optional radiopathological follow-up depending on the cardiologist's decision. A second DAP action level of 250 Gy cm(2) corresponding to the 3 Gy skin dose would imply a systematic follow-up. Dose reference levels - 71.3 Gy cm(2) for diagnostic and 106.0 Gy cm(2) for therapeutic procedures - were derived from the 75 percentile of the DAP distributions. As a conclusion, we propose that total DAP is registered in patient's record file, as it can serve to improve the follow-up of patients for radiation-induced skin injuries. [less ▲]

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See detailLarge-scale palaeontological data analysis of the conodonts fossil history
Martínez-Pérez, Carlos; Botella, Héctor; Cascales - Miñana, Borja ULg

Conference (2010, June)

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See detailLarge-scale pattern of genetic differentiation within African rainforest trees: insights on the roles of ecological gradients and past climate changes on the evolution of Erythrophleum spp (Fabaceae)
Duminil, Jérôme; Brown, Richard P.; Ewédjè, Eben-Ezer BK. et al

in BMC Evolutionary Biology (2013), 13

Background: The evolutionary events that have shaped biodiversity patterns in the African rainforests are still poorly documented. Past forest fragmentation and ecological gradients have been advocated as ... [more ▼]

Background: The evolutionary events that have shaped biodiversity patterns in the African rainforests are still poorly documented. Past forest fragmentation and ecological gradients have been advocated as important drivers of genetic differentiation but their respective roles remain unclear. Using nuclear microsatellites (nSSRs) and chloroplast non-coding sequences (pDNA), we characterised the spatial genetic structure of Erythrophleum (Fabaceae) forest trees in West and Central Africa (Guinea Region, GR). This widespread genus displays a wide ecological amplitude and taxonomists recognize two forest tree species, E. ivorense and E. suaveolens, which are difficult to distinguish in the field and often confused. Results: Bayesian-clustering applied on nSSRs of a blind sample of 648 specimens identified three major gene pools showing no or very limited introgression. They present parapatric distributions correlated to rainfall gradients and forest types. One gene pool is restricted to coastal evergreen forests and corresponds to E. ivorense; a second one is found in gallery forests from the dry forest zone of West Africa and North-West Cameroon and corresponds to West-African E. suaveolens; the third gene pool occurs in semi-evergreen forests and corresponds to Central African E. suaveolens. These gene pools have mostly unique pDNA haplotypes but they do not form reciprocally monophyletic clades. Nevertheless, pDNA molecular dating indicates that the divergence between E. ivorense and Central African E. suaveolens predates the Pleistocene. Further Bayesian-clustering applied within each major gene pool identified diffuse genetic discontinuities (minor gene pools displaying substantial introgression) at a latitude between 0 and 2°N in Central Africa for both species, and at a longitude between 5° and 8°E for E. ivorense. Moreover, we detected evidence of past population declines which are consistent with historical habitat fragmentation induced by Pleistocene climate changes. Conclusions: Overall, deep genetic differentiation (major gene pools) follows ecological gradients that may be at the origin of speciation, while diffuse differentiation (minor gene pools) are tentatively interpreted as the signature of past forest fragmentation induced by past climate changes. [less ▲]

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See detailLarge-Scale Phenotypic Analysis Reveals Identical Contributions To Cell Functions Of Known And Unknown Yeast Genes
Bianchi, Mm.; Ngo, S.; Vandenbol, Micheline ULg et al

in Yeast (2001), 18(15),

Detailed reference viewed: 13 (0 ULg)