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See detailA new map of the lava flow field of Nyamulagira (D.R. Congo) from satellite imagery
Smets, Benoît; Wauthier, Christelle ULg; d'Oreye, N.

in Journal of African Earth Sciences (2010)

Detailed reference viewed: 40 (10 ULg)
See detailA new Mars ionosphere/airglow Model between 60 and 500 km altitude
Witasse, Oliver; Blelly, P.; Lilensten, Jean et al

in Proceedings of the Workshop “Mars Atmosphere Modelling and Observations” (2003)

Detailed reference viewed: 14 (0 ULg)
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See detailNew mass spectrometry based methodology to sequence a whole snake venom
Echterbille, Julien ULg; Boulanger, Madeleine; Degueldre, Michel ULg et al

Poster (2014, February 10)

Animal venoms are complex chemical cocktails, comprising wide ranges of biologically active reticulated peptides that target with high selectivity and efficacy varieties of membrane receptors. Assuming ... [more ▼]

Animal venoms are complex chemical cocktails, comprising wide ranges of biologically active reticulated peptides that target with high selectivity and efficacy varieties of membrane receptors. Assuming the fact that each of the 170,000 venomous species reported can produce more than 250 bioactive toxins, at least 40,000,000 bioactive peptides and proteins may be discovered. Among the four described species of mambas, Eastern Jameson’s mamba (Dendroaspis jamesonii kaimosae) venom is the less characterized since only 9 peptides are referenced in database. This work aims at developing a new strategy devoted to the deep analysis of animal venoms. Our approach consists in a first separation of the venom using cation exchange chromatography. Each primary fraction is then purified a second time by classical RP-HPLC. A total of 328 fractions, containing amongst 1 and 4 toxins, are finally collected. MALDI-MS analysis of each fraction is done in order (1) to obtain information about masses and (2) to obtain sequences of toxins thanks to MALDI-In Source Decay (ISD) dissociation coupled with on MALDI target plate reduction of the peptides. ISD has already been demonstrated efficient for toxin sequencing, and especially when using 1,5-DAN as reducing matrix. ISD yields to sequences that cover more than 50% of peptide sequences by series of singly charged c-type ions. Thanks to this methodology, we were able to obtain 85% of satisfactory results i.e. spectra giving quite long tags of amino acids (up to 20 residues). As a way to validate our method, a tag coming from ISD spectrum interpretation has found a match in database for an Eastern Jameson’s mamba toxin. The global sequence has then been obtained by extrapolation on the ISD spectrum. Since ISD spectra are simpler than classical MS/MS spectra, automation of spectra interpretation, difficult with other fragmentation techniques (CID, ETD…), is implementable. In the near future, sequences obtained with this approach will be used to direct tests of biological activity through sequence homologies with already known ligands for different kinds of membrane receptors. [less ▲]

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See detailThe new Mayo clinic equation for estimating glomerular filtration rate
Delanaye, Pierre ULg; Krzesinski, Jean-Marie ULg

in Annals of Internal Medicine (2005), 142(8), 679-680

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See detailNew measures of the quality and of the reliability of fits applied to forward hadronic data at t = 0
Cudell, Jean-René ULg; Kang, Kyungsik; Ezhela, V. V. et al

in H.M. Fried; Gabellini, Y.; Muller, B. (Eds.) NON - PERTURBATIVE QCD (2001)

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See detailA new mechanism of upwelling generated filaments based on potential vorticity balance
Troupin, Charles ULg; Mason, Evan; Beckers, Jean-Marie ULg et al

Poster (2009, April 19)

Detailed reference viewed: 11 (1 ULg)
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See detailA new mechanism of upwelling generated filaments based on potential vorticity balance
Troupin, Charles ULg; Mason, Evan; Beckers, Jean-Marie ULg et al

Conference (2009, April 27)

Detailed reference viewed: 8 (0 ULg)
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See detailNew mesh generation developments in GMSH
Remacle, Jean-François; Johnen, Amaury ULg; Lambrechts, Jonathan et al

in Actes du 11e Colloque National en Calcul des Structures (CSMA 2013), Giens, France (2013)

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See detailNew method for 3D reconstruction of the human cranial vault from CT-scan data
Laurent, Cédric ULg; JOLIVET, Erwan; HODEL, Jérôme et al

in Medical Engineering & Physics (2011), 33

This study presents a new method for the 3D reconstruction of the human cranial vault from routine Computed Tomography (CT) data. The reconstruction method was based on the conceptualization of the shape ... [more ▼]

This study presents a new method for the 3D reconstruction of the human cranial vault from routine Computed Tomography (CT) data. The reconstruction method was based on the conceptualization of the shape of the cranial vault with a parametric description. An initialization was first realized with the identification of anatomical landmarks and contours on Digitally Reconstructed Radiographs (DRR) in order to obtain a pre-personalized reconstruction. Then an optimization of the reconstruction was performed to segment the internal and external surfaces of the cranial vault for thickness computation. The method was validated by comparing final reconstructions issued from our approach and from a manual sliceby- slice segmentation method on ten CT-scans. Errors were comparable to the CT image resolution, and less than 2 min were dedicated to the operatordependant marking step. The reconstruction of internal and external surfaces of the cranial vault allows quantifying and visualizing of thickness throughout the cranial vault. This thickness mapping is useful for clinical purposes as additional pre-surgical information. Moreover, this study constitutes a first step in the personalized characterization of skull resistance directly from routine exams. [less ▲]

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See detailNew method for a two-step hydrolysis and chromatographic analysis of pectin neutral sugar chains
Garna, H.; Mabon, N.; Wathelet, Bernard ULg et al

in Journal of Agricultural and Food Chemistry (2004), 52(15), 4652-4659

A new method for the determination of the main neutral sugars in pectin has been developed. The sample preparation involves a mild chemical attack followed by an enzymatic hydrolysis. The completeness and ... [more ▼]

A new method for the determination of the main neutral sugars in pectin has been developed. The sample preparation involves a mild chemical attack followed by an enzymatic hydrolysis. The completeness and nondestructive character of the method are demonstrated by comparison of the results obtained with different acids such as H2SO4, HCI, and trifluoroacetic acid (TFA) at different concentrations (2, 1, or 0.2 M) at two temperatures (80 or 100 degreesC). The chemical hydrolysis of pectin neutral sugar chains with strong acid (1 or 2 M) and high temperature (100 degreesC) shows that the liberation of the pectin sugars is not realized at the same rate for each sugar. Different optimum conditions are thus obtained. However, the chemical pectin hydrolysis with 0.2 M TFA at 80 degreesC is characterized by the liberation of pectin neutral sugar side chains without any degradation within 72 h of hydrolysis. Under these conditions, the liberation of some pectin sugars, essentially galactose, glucose, and rhamnose, was not complete. An enzymatic hydrolysis is necessary to obtain a complete release of all the sugars. The combination of the two treatments, a chemical hydrolysis realized with diluted acid (0.2 M) for 72 h at low temperature (80 degreesC) on one hand and an enzymatic hydrolysis on the other hand, allow a total liberation of pectin sugars. The quantitative analysis of the carbohydrates is realized with accuracy, high selectivity, and sensitivity with high-performance anion-exchange chromatography with pulsed-amperometric detection. The sugars can be analyzed without any derivatization with a limit of quantification of 0.1 mM. [less ▲]

Detailed reference viewed: 48 (6 ULg)
See detailA new method for absolute quantification of allergens in food: the "Heavy Peptides method".
Fourdrilis, Séverine; Bourgeon, Cédric; Kirsch, Stéphanie ULg et al

Poster (2008)

Detailed reference viewed: 33 (5 ULg)
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See detailNew method for characterizing highly disulfide-bridged peptides in complex mixtures: Application to toxin identification from crude venoms
Quinton, Loïc ULg; Demeure, Kevin ULg; Dobson, Rowan ULg et al

in Journal of Proteome Research (2007), 6(8), 3216-3223

Animal venoms are highly complex mixtures that can contain many disulfide-bridged toxins. This work presents an LC-MALDI approach allowing (1) a rapid classification of toxins according to their number of ... [more ▼]

Animal venoms are highly complex mixtures that can contain many disulfide-bridged toxins. This work presents an LC-MALDI approach allowing (1) a rapid classification of toxins according to their number of disulfide bonds and (2) a rapid top-down sequencing of the toxins using a new MALDI matrix enhancing in-source decay (ISD). The crude venom is separated twice by LC: the fractions of the first separation are spotted on the MALDI matrix alpha-cyano-4-hydroxycinnamic acid (CHCA) and the others using 1,5-diaminonaphthalene (1,5-DAN). CHCA spots are more convenient for obtaining a precise mass fingerprint of a large number of peptides; however, the analysis of 1,5-DAN spots allows the number of disulfide bridges to be counted owing to their partial in-plume reduction by this particular matrix. Subsequently, the disulfide bonds of all peptides present in the crude venom were reduced by an excess of tris(carboxyethyl)phosphine before the LC separation and were subjected to the same analysis in CHCA and 1,5-DAN. Toxins were sequenced using a TOF/TOF analysis of metastable fragments from CHCA spots and ISD fragmentation from 1,5-DAN spots. Novel conotoxin sequences were found using this approach. The use of 1,5-DAN for ISID top-down sequencing is also illustrated for higher molecular weight toxins such as snake cardiotoxins and neurotoxins (>6500 Da), where sequence coverage >70% is obtained from the c-ion series. [less ▲]

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See detailA new method for computing the derivatives of the mean and amplitude of physiological variables with respect to the parameters of a cardiovascular system model
Pironet, Antoine ULg; Dauby, Pierre ULg; Revie, James A. et al

in Minimally Invasive Therapy & Allied technologies : Official Journal of the Society for Minimally Invasive Therapy (2013), 22

Detailed reference viewed: 23 (6 ULg)
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See detailNew method for establishing a network of operational warning of Septoria leaf blotch disease in winter wheat
El Jarroudi, Moussa ULg; Giraud, Frédéric; Defosse, Philippe et al

Poster (2011, August 06)

Detailed reference viewed: 14 (0 ULg)
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See detailNew method for establishing a network of operational warning of Septoria leaf blotch disease in winter wheat
El Jarroudi, Moussa ULg; Giraud, Frédéric; Delfosse, Philippe et al

in Phytopathology (2011), 101

A mechanistic model, PROCULTURE, based on commonly available meteorological data and assessing in real time the risk of progression of septoria leaf blotch disease on winter wheat has been developed in ... [more ▼]

A mechanistic model, PROCULTURE, based on commonly available meteorological data and assessing in real time the risk of progression of septoria leaf blotch disease on winter wheat has been developed in Belgium and the Grand-Duchy of Luxemburg (GDL) to limit fungicide use. However, the reliability of meteorological stations used for the warning system varies according to the distance to the fields. A weather analysis based on the Fourier transform highlighted a great difference in the intraday variation between two sites in the GDL (Everlange and Reuland). The correlation between these two sites is very high for the hourly temperature (R = 0.96), and for the hourly relative humidity (RH) (R = 0.86), (P < 0.05). However, the intraday variation (<11 hours) highlights contrasts for a given meteorological parameter. Hence, the correlation between temperature or RH decreased respectively from 0.96 to 0.43 and from 0.86 to 0.30. The comparison between infection conditions given by PROCULTURE using the Fourier transform, shows: (i) a positive but weak correlation between temperature at Reuland and Everlange (R = 0.64), (ii) a good correlation between RH for these two sites (R = 0.86), and (iii) a contrasted difference for rain (R = 0.27), (P < 0.05). This Fourier transform based method enables to take into account the RH and temperature variation related to topography levels in the warning system and to understand and explain the variation in disease expression between a plateau and a valley bottom or between North and South slopes. [less ▲]

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See detailA new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.
Barker, Jennifer M.; Charlier, Thierry D.; Ball, Gregory F. et al

in PloS one (2013), 8(5), 63692

Systemic injection of a thymidine analogue such as bromodeoxyuridine (BrdU) in vertebrates is commonly used to detect and study cell production during development, adulthood, and pathology, particularly ... [more ▼]

Systemic injection of a thymidine analogue such as bromodeoxyuridine (BrdU) in vertebrates is commonly used to detect and study cell production during development, adulthood, and pathology, particularly in studies of adult neurogenesis. Although researchers are applying this technique to multiple species in various physiological conditions, the rate of BrdU clearance from the serum remains unknown in most cases. Changes in this clearance rate as a function of the species, sex or endocrine condition could however profoundly affect the interpretation of the results. We describe a rapid, sensitive, but simple bioassay for post-injection detection and quantification of BrdU in serum. This procedure was shown to be suitable for determining the length of time a thymidine analogue remains in the bloodstream of one avian species and seems applicable to any vertebrate provided sufficiently large blood samples can be collected. This technique was used to demonstrate that, in canaries, BrdU injected at a dose of 100 mg/kg is no longer available for incorporation into DNA between 30 and 60 min post-injection, a delay shorter than anticipated based on the available literature. Preliminary data suggest a similar fast clearance in Japanese quail and mice. [less ▲]

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See detailNew method for nitroxyl radical synthesis
Claes, M.; Detrembleur, Christophe ULg; Hoebeke, Maryse ULg et al

Conference (2002)

Detailed reference viewed: 11 (1 ULg)
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See detailNew method for photovoltaic solar cell physical parameters extraction
Aazou, Safae ULg; Ibral, A.; Assaid, M. et al

Conference (2011)

Photovoltaic energy is one of the most important renewable energies. This type of energy, unlike other energy sources, is clean, safe, and abundant. The photovoltaic solar energy is based on the ... [more ▼]

Photovoltaic energy is one of the most important renewable energies. This type of energy, unlike other energy sources, is clean, safe, and abundant. The photovoltaic solar energy is based on the conversion of sunlight into direct current by solar cells. In order to increase the efficiency of the photovoltaic conversion and for a better understanding of the solar cell behavior, an accurate knowledge of the cell physical parameters is required. In this work, the solar cell is considered as a generator and the one-diode equivalent circuit is retained. This electronic circuit modeling the solar cell contains a diode with its reverse saturation current and its ideality factor, parasitic series and shunt resistances and a photocurrent generator. In this paper, a new physical parameters extraction method is presented, for the first time to our knowledge, which is based on the current-voltage characteristics and on the analytical expression of the output voltage given in term of the Lambert W function. This method gives all the physical parameters without any approximation or introduction of initial values. To test the efficiency of the presented method, a comparative study with other extraction methods is done. The obtained results are in good agreement. [less ▲]

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See detailA New Method for Quality by Design Robust Optimization in Liquid Chromatography
Debrus, Benjamin ULg; Lebrun, Pierre ULg; Rozet, Eric ULg et al

in LC-GC Europe (2013)

A new method to optimize liquid chromatography (LC) methods using a Quality by Design (QbD) approach is presented. This method is based on the use of design of experiments (DOE) and independent component ... [more ▼]

A new method to optimize liquid chromatography (LC) methods using a Quality by Design (QbD) approach is presented. This method is based on the use of design of experiments (DOE) and independent component analysis (ICA) to accurately estimate the modelled responses (that is, the retention times at the beginning, the apex, and the end) of each peak, even for coeluted peaks. The modelling of these responses usesmultiple linear regressions, while the propagation of the error affecting the responses and coming from the models is carried out by Monte Carlo simulation. The design space is determined as the region of assay factors where the probability to reach baseline-resolved peaks is higher than the desired level of quality. This method was applied to the optimization of the separation of nine compounds in a mixture, yielding the design space and the demonstration of robustness of the method. Finally, the method was validated. [less ▲]

Detailed reference viewed: 100 (16 ULg)
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See detailA new method for reconstruction of the vertical electron density distribution in the upper ionosphere and plasmasphere
Stankov, Stanimir; Jakowski, Norbert; Muhtarov, Plamen et al

in Journal of Geophysical Research. Space Physics (2003), 108(A51164), 1164

Detailed reference viewed: 22 (4 ULg)