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See detailNMR structure of Citrobacter freundii AmpD, comparison with bacteriophage T7 lysozyme and homology with PGRP domains.
Liepinsh, Edvards; Genereux, Catherine ULg; Dehareng, Dominique ULg et al

in Journal of Molecular Biology (2003), 327(4), 833-42

AmpD is a bacterial amidase involved in the recycling of cell-wall fragments in Gram-negative bacteria. Inactivation of AmpD leads to derepression of beta-lactamase expression, presenting a major pathway ... [more ▼]

AmpD is a bacterial amidase involved in the recycling of cell-wall fragments in Gram-negative bacteria. Inactivation of AmpD leads to derepression of beta-lactamase expression, presenting a major pathway for the acquisition of constitutive antibiotic resistance. Here, we report the NMR structure of AmpD from Citrobacter freundii (PDB accession code 1J3G). A deep substrate-binding pocket explains the observed specificity for low molecular mass substrates. The fold is related to that of bacteriophage T7 lysozyme. Both proteins bind zinc at a conserved site and require zinc for amidase activity, although the enzymatic mechanism seems to differ in detail. The structure-based sequence alignment identifies conserved features that are also conserved in the eukaryotic peptidoglycan recognition protein (PGRP) domains, including the zinc-coordination site in several of them. PGRP domains thus belong to the same fold family and, where zinc-binding residues are conserved, may have amidase activity. This hypothesis is supported by the observation that human serum N-acetylmuramyl-L-alanine amidase seems to be identical with a soluble form of human PGRP-L. [less ▲]

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See detailNMR Studies of Solutions of Actinide Ions and their Complexes. Relaxation and Spectroscopy
Braekers, Damien; Peters, Christelle; Vidick, Geoffrey ULg et al

Poster (2008, July)

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See detailNMR studies on radioactive actinide complexes. A completely foolish idea?
Vidick, Geoffrey ULg; Bouslimani, Nouri; Desreux, Jean-François ULg

Conference (2010, November 26)

The complete separation of actinides and lanthanides by solvent extraction is an important step in the reprocessing of nuclear wastes. The trivalent ions of these two families of f elements have very ... [more ▼]

The complete separation of actinides and lanthanides by solvent extraction is an important step in the reprocessing of nuclear wastes. The trivalent ions of these two families of f elements have very similar properties and it is only recently that an effective separation method has been developed. It is assumed that this separation can be achieved because of small differences in the covalency of the coordination bonds formed by the f ions but this has not been proved so far. We embarked into the first NMR study of the actinide complexes with the hope of better unravelling their solution structures and of clarifying the role of covalency. Nuclear magnetic resonance has nearly never been used for investigating actinide ions and their complexes not only because of their radioactivity and their toxicity but also because of the lack of dedicated spectrometers. It was also assumed that paramagnetism would cause excessive line broadenings. Many difficulties had thus to be overcome and the first step in our work was to show that well-resolved NMR spectra of actinide complexes (U to Cm) could indeed be obtained. Relatively narrow resonances have been observed for a variety of ions in different oxidation states provided their complexes are stable, symmetric and rigid. We used several advanced NMR techniques in order to fully characterize the actinide ions and their chelates in water or in organic solvents. The dispersion of the longitudinal relaxation time T1 of solvent nuclei with the magnetic field (NMRD) yields information on the magnetic properties and on the dynamic behaviour of paramagnetic species; 17O NMR allows the measurement of the water exchange times and 1H and 13C spectra yield information on the solution structures of the complexes. It will be shown that the paramagnetic shifts induced by the actinide ions originate from both a through space dipolar contribution and a through bonds contact contribution. The latter gives access to delocalized unpaired electron densities that are directly related to the covalency of the metal-ligand bonds. However, electron densities can only be obtained after separation of the two contributions. This could be accomplished thanks to variable temperature studies. In another approach, new ligands with rigid aliphatic substituents have been synthesized and contact contribution were deduced with the assumption that delocalization does not proceed to the 1H nuclei most removed from the metal center. This study has been extended to actinides in the trivalent, tetravalent and hexavalent states and new ligands have been synthesized in order to increase the magnetic anisotropy while keeping the symmetry and the rigidity needed to simplify the NMR analyses. [less ▲]

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See detailNMR study of ginkgolides by a suited ROESY approach
Grodent, Denis ULg; Llabres, Gabriel ULg

Poster (1992, August 15)

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See detailNMR study of surfactant molecules intercalated in montmorillonite and silylated montmorillonite
Grandjean, Jean ULg; Bujdak, Janec; Komadel, Peter

in Clay Minerals (2003), 38

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See detailNo accelerated rate of protein evolution in male-biased Drosophila pseudoobscura genes.
Metta, Muralidhar ULg; Gudavalli, Rambabu; Gibert, Jean-Michel et al

in Genetics (2006), 174(1), 411-20

Sexually dimorphic traits are often subject to diversifying selection. Genes with a male-biased gene expression also are probably affected by sexual selection and have a high rate of protein evolution. We ... [more ▼]

Sexually dimorphic traits are often subject to diversifying selection. Genes with a male-biased gene expression also are probably affected by sexual selection and have a high rate of protein evolution. We used SAGE to measure sex-biased gene expression in Drosophila pseudoobscura. Consistent with previous results from D. melanogaster, a larger number of genes were male biased (402 genes) than female biased (138 genes). About 34% of the genes changed the sex-related expression pattern between D. melanogaster and D. pseudoobscura. Combining gene expression with protein divergence between both species, we observed a striking difference in the rate of evolution for genes with a male-biased gene expression in one species only. Contrary to expectations, D. pseudoobscura genes in this category showed no accelerated rate of protein evolution, while D. melanogaster genes did. If sexual selection is driving molecular evolution of male-biased genes, our data imply a radically different selection regime in D. pseudoobscura. [less ▲]

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See detailNo association between C-reactive protein gene polymorphisms and decrease of C-reactive protein serum concentration after infliximab treatment in Crohn's disease
Willot, S.; Vermeire, S.; Ohresser, M. et al

in Pharmacogenetics and Genomics (2006), 16(1), 37-42

We recently showed an association between the FCGR3A V/F polymorphism and the biological response [assessed on the basis of a C-reactive protein (CRP) concentration decrease] to infliximab in Crohn's ... [more ▼]

We recently showed an association between the FCGR3A V/F polymorphism and the biological response [assessed on the basis of a C-reactive protein (CRP) concentration decrease] to infliximab in Crohn's disease. The CRP and FCGR3A genes are located on the same 1q23 locus. The present study aimed: 0) to exclude a linkage disequilibrium (LD) between the two genes and 00 to study the association between CRP polymorphisms and the response to infliximab, particularly the decrease in CRP after treatment, in Crohn's disease patients. FCGR3A (V/F) polymorphism and three CRP polymorphisms (- 717G/A, 1444C/T, CRP 4A/G) were determined in 206 healthy blood donors and 189 Crohn's disease patients who had received infliximab for either refractory luminal or fistulizing Crohn's disease. Clinical response was defined as complete, partial or absent according to the same definition as in controlled trials. The biological response was defined on the basis of CRP decrease. There was no LID between CRP and FCGR3A in healthy donors or Crohn's disease patients. CRP polymorphisms had no impact on CRP decrease after infliximab. The proportions of Crohn's disease having a positive clinical or biological response were not statistically different among the various genotypes of CRP polymorphisms. There was no LD between CRP and FCGR3A polymorphisms. CRP polymorphisms were not associated with the response to infliximab in Crohn's disease. Pharmacogenetics and Genomics 16:37-42. (c) 2006 Lippincott Williams [less ▲]

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See detailNo delay in the court – divorce in France and Belgium
Bastard, Benoit; Delvaux, David ULg; Schoenaers, Frédéric ULg et al

Conference (2012, July)

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See detail"Lo no dicho en 'Corazón de Kaláshnikov', de Alejandro Páez Varela. Personajes, causalidades, parataxis."
Vanden Berghe, Kristine ULg

in Literatura mexicana. Revista semestral del centro de estudios literarios. (2014), 25(2), 93-110

A stylistic analysis of Corazón de Kaláshnikov (2009) by Alejandro Páez Varela highlights that the novel is extremely elliptic: the focalization of the characters is external, the causal relations are ... [more ▼]

A stylistic analysis of Corazón de Kaláshnikov (2009) by Alejandro Páez Varela highlights that the novel is extremely elliptic: the focalization of the characters is external, the causal relations are implicit and the style is very much paratactic. This seems to correspond to an ethical choice for not participating in the sensationalist representations of the violence in Ciudad Juárez, an option Páez Varela shares with the Bolaño of 2666, specifically the part about the femicides. At the same time, Corazón de Kaláshnikov seems to be typical of a certain style practiced in the north of Mexico, tense and sober, and related to a peculiar geographic and climatic environment. [less ▲]

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See detailNo earthquake with characteristic slip on the North Anatolian Fault, Turkey
Hubert, Aurelia ULg; Fraser, J.; Drab, L. et al

in Geophysical Research Abstracts (2009, April)

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See detailNo effect of eletriptan administration during the aura phase of migraine
Olesen, J.; Diener, H. C.; Schoenen, Jean ULg et al

in European Journal of Neurology (2004), 11(10), 671-677

Migraine aura is a warning sign readily recognized by patients. From the onset of aura it takes 30-60 min before the headache phase starts. Administration of acute medication during aura should provide ... [more ▼]

Migraine aura is a warning sign readily recognized by patients. From the onset of aura it takes 30-60 min before the headache phase starts. Administration of acute medication during aura should provide sufficient time to achieve therapeutic plasma levels, counteracting the headache. To test this hypothesis we evaluated the efficacy of eletriptan 80 mg taken during aura. Patients met International Headache Society diagnostic criteria for migraine with aura, with an attack frequency of at least one per month and with aura occurring in >50% of recent attacks. Of 123 patients randomized, 87 (71%) were treated with a double-blind, one attack, during the aura phase before headache, dose of either eletriptan 80 mg (n = 43; 74% female; mean age, 40 years), or placebo (n = 44; 82% female; mean age, 40 years). The primary outcome measure was the proportion of patients not developing moderate-to-severe headache within 6 h post-dose. There was no significant difference in the proportion of patients developing moderate-to-severe headache on eletriptan (61%) versus placebo (46%). Eletriptan was well tolerated and did not prolong the aura phase. Typical transient triptan adverse events were observed; most were mild-to-moderate in intensity. This study confirms the findings of two studies showing that triptans are ineffective but safe when given during the migraine aura phrase. [less ▲]

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See detailNo effect of parity on concentrations of bovine pregnancy associated glycoprotein (PAG) measurement by radioimmunoassay.
Ayad, A.; Melo de Sousa, Noelita ULg; Iguer-Ouada, M. et al

in Reproduction in Domestic Animals (2008), 43(Suppl 5), 90

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See detailNo efficiency of the lateral system on nocturnal feeding in the European sea bass (Dicentrarchus labrax L.)
Faucher, Karine ULg; Dutto, Gilbert; Covès, Denis et al

in Aquaculture (2006), 252(2-4), 462-475

In order to evaluate the effect and consequence of lateral system inactivation on fish nocturnal feeding, the differential growth of groups of European sea bass maintained in different rearing conditions ... [more ▼]

In order to evaluate the effect and consequence of lateral system inactivation on fish nocturnal feeding, the differential growth of groups of European sea bass maintained in different rearing conditions were compared. Whereas some fish with intact lateral system (placebo fish) were placed under a photoperiod of 12-L: 12-D, other placebo fish were kept in the dark. In the same way, fish deprived of lateral system by section of their lateral system nerves and antibiotic treatment were placed under a photoperiod of 12-L :12-D and the others in the dark. For each of these four rearing conditions, two sets of experiment were realized. Percent mortality, feed rhythm, averaged daily feed demand, specific growth rate and feed efficiency were compared among these four groups of fish. After four months of experiment, results revealed that, under a photoperiod of 12-L: 12-D, fish showed a diurnal feed rhythm whereas no rhythm appeared in fish kept in the dark. In addition, as reported by other authors, the average daily feed demand, the quantity of ingested food and specific growth rate were greater in fish maintained under a photoperiod of 12-L :12-D than those kept in the dark. The fish lateral system inactivation did not affect mortality, feed intake, specific growth rate or feed efficiency. These results demonstrated that lateral system is not the major sensory organ leading to European sea bass nocturnal feeding; chemoreception system undoubtedly taking over. If the olfactory system explains equal feed intake between placebo and treated fish, the greater specific growth rate in treated than in placebo fish indicates the action of another mechanism, such as a "booster effect" of antibiotics used for lateral system inactivation on fish. [less ▲]

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See detailNo evidence of age-related increases in unconscious plagiarism during free recall.
Perfect, Timothy J.; Defeldre, Anne-Catherine; Elliman, Rachel et al

in Memory (2011)

In 3 experiments younger and older participants took part in a group generation task prior to a delayed recall task. In each, participants were required to recall the ideas that they had generated ... [more ▼]

In 3 experiments younger and older participants took part in a group generation task prior to a delayed recall task. In each, participants were required to recall the ideas that they had generated, avoiding plagiarism errors. All studies showed the same pattern: older adults did not plagiarise their partners any more than younger adults did. However, older adults were more likely than younger adults to intrude with entirely novel items not previously generated by anyone. These findings stand in opposition to the single previous demonstration of age-related increases in plagiarism during recall. [less ▲]

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See detailNo evidence of human papillomavirus DNA in breast carcinoma in Tunisian patients
Hachana, Mohamed Ridha ULg; Ziadi, Sonia; Amara, Khaled et al

in Breast (Edinburgh, Scotland ) (2010), 19(6), 541-544

Detailed reference viewed: 9 (2 ULg)