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See detailThe PAX2-null immunophenotype defines multiple lineages with common expression signatures in benign and neoplastic oviductal epithelium.
Ning, Gang; Bijron, Jonathan G.; Yamamoto, Yusuke et al

in The Journal of pathology (2014)

The oviducts contain high-grade serous cancer (HGSC) precursors (serous tubal intraepithelial neoplasia or STINs), which are gamma-H2AXp - and TP53 mutation-positive. Although they express wild-type p53 ... [more ▼]

The oviducts contain high-grade serous cancer (HGSC) precursors (serous tubal intraepithelial neoplasia or STINs), which are gamma-H2AXp - and TP53 mutation-positive. Although they express wild-type p53, secretory cell outgrowths (SCOUTs) are associated with older age and serous cancer; moreover, both STINs and SCOUTs share a loss of PAX2 expression (PAX2n ). We evaluated PAX2 expression in proliferating adult and embryonic oviductal cells, normal mucosa, SCOUTs, Walthard cell nests (WCNs), STINs, and HGSCs, and the expression of genes chosen empirically or from SCOUT expression arrays. Clones generated in vitro from embryonic gynaecological tract and adult Fallopian tube were Krt7p /PAX2n /EZH2p and underwent ciliated (PAX2n /EZH2n /FOXJ1p ) and basal (Krt7n /EZH2n /Krt5p ) differentiation. Similarly, non-ciliated cells in normal mucosa were PAX2p but became PAX2n in multi-layered epithelium undergoing ciliated or basal (WCN) cell differentiation. PAX2n SCOUTs fell into two groups: type 1 were secretory or secretory/ciliated with a 'tubal' phenotype and were ALDH1n and beta-cateninmem (membraneous only). Type 2 displayed a columnar to pseudostratified (endometrioid) phenotype, with an EZH2p , ALDH1p , beta-cateninnc (nuclear and cytoplasmic), stathminp , LEF1p , RCN1p , and RUNX2p expression signature. STINs and HGSCs shared the type 1 immunophenotype of PAX2n , ALDH1n , beta-cateninmem , but highly expressed EZH2p , LEF1p , RCN1p , and stathminp . This study, for the first time, links PAX2n with proliferating fetal and adult oviductal cells undergoing basal and ciliated differentiation and shows that this expression state is maintained in SCOUTs, STINs, and HGSCs. All three entities can demonstrate a consistent perturbation of genes involved in potential tumour suppressor gene silencing (EZH2), transcriptional regulation (LEF1), regulation of differentiation (RUNX2), calcium binding (RCN1), and oncogenesis (stathmin). This shared expression signature between benign and neoplastic entities links normal progenitor cell expansion to abnormal and neoplastic outgrowth in the oviduct and exposes a common pathway that could be a target for early prevention. Copyright (c) 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. [less ▲]

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See detailPax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates alpha-cell generation by repressing arx gene expression.
Djiotsa, Joachim; Verbruggen, Vincianne; Giacomotto, Jean et al

in BMC Developmental Biology (2012), 12

BACKGROUND: Genetic studies in mouse have demonstrated the crucial function of PAX4 in pancreatic cell differentiation. This transcription factor specifies beta- and delta-cell fate at the expense of ... [more ▼]

BACKGROUND: Genetic studies in mouse have demonstrated the crucial function of PAX4 in pancreatic cell differentiation. This transcription factor specifies beta- and delta-cell fate at the expense of alpha-cell identity by repressing Arx gene expression and ectopic expression of PAX4 in alpha-cells is sufficient to convert them into beta-cells. Surprisingly, no Pax4 orthologous gene can be found in chicken and Xenopus tropicalis raising the question of the function of pax4 gene in lower vertebrates such as in fish. In the present study, we have analyzed the expression and the function of the orthologous pax4 gene in zebrafish. RESULTS: pax4 gene is transiently expressed in the pancreas of zebrafish embryos and is mostly restricted to endocrine precursors as well as to some differentiating delta- and epsilon-cells but was not detected in differentiating beta-cells. pax4 knock-down in zebrafish embryos caused a significant increase in alpha-cells number while having no apparent effect on beta- and delta-cell differentiation. This rise of alpha-cells is due to an up-regulation of the Arx transcription factor. Conversely, knock-down of arx caused to a complete loss of alpha-cells and a concomitant increase of pax4 expression but had no effect on the number of beta- and delta-cells. In addition to the mutual repression between Arx and Pax4, these two transcription factors negatively regulate the transcription of their own gene. Interestingly, disruption of pax4 RNA splicing or of arx RNA splicing by morpholinos targeting exon-intron junction sites caused a blockage of the altered transcripts in cell nuclei allowing an easy characterization of the arx- and pax4-deficient cells. Such analyses demonstrated that arx knock-down in zebrafish does not lead to a switch of cell fate, as reported in mouse, but rather blocks the cells in their differentiation process towards alpha-cells. CONCLUSIONS: In zebrafish, pax4 is not required for the generation of the first beta- and delta-cells deriving from the dorsal pancreatic bud, unlike its crucial role in the differentiation of these cell types in mouse. On the other hand, the mutual repression between Arx and Pax4 is observed in both mouse and zebrafish. These data suggests that the main original function of Pax4 during vertebrate evolution was to modulate the number of pancreatic alpha-cells and its role in beta-cells differentiation appeared later in vertebrate evolution. [less ▲]

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See detailPAX5/IGH rearrangement is a recurrent finding in a subset of aggressive B-NHL with complex chromosomal rearrangements
Poppe, B.; De Paepe, P.; Michaux, L. et al

in Genes Chromosomes & Cancer (2005), 44(2), 218-223

We present an extensive characterization of 10 B-cell lymphomas with a t(9; 14)(p I 3;q32). The presence of the PAX5/IGH gene rearrangement was demonstrated by fluorescence in situ hybridization (FISH ... [more ▼]

We present an extensive characterization of 10 B-cell lymphomas with a t(9; 14)(p I 3;q32). The presence of the PAX5/IGH gene rearrangement was demonstrated by fluorescence in situ hybridization (FISH) using a validated probe set, whereas complex karyotypic changes were reassessed by multiplex-FISH (M-FISH). Pathologic and clinical review revealed the presence of this rearrangement in 4 histiocyte-rich, T-cell-rich B-cell lymphomas (HRTR-BCLs) and 2 posttransplantation diffuse large B-cell lymphomas (PTLD-DLBCLs). In contrast to initial observations describing this translocation in lymphoplasmacytic lymphoma (LPL) and LPL-derived large B-cell lymphoma, our data showed a wide morphologic and clinical spectrum associated with the PAX5/IGH rearrangement, pointing to an association between this aberration and a subset of de novo DLBCLs presenting with advanced disease and adverse prognosis. In addition, the recurrent incidence of this rearrangement in both HRTR-BCL (4 cases) and PTLD-DLBCL (2 cases) was previously unrecognized and is intriguing. (c) 2005 Wiley-Liss, Inc. [less ▲]

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See detailThe Pax6b homeodomain is dispensable for pancreatic endocrine cell differentiation in zebrafish.
Verbruggen, Vincianne; Ek, Olivier; Georlette, Daphne et al

in Journal of Biological Chemistry (2010), 285(18), 13863-73

Pax6 is a well conserved transcription factor that contains two DNA-binding domains, a paired domain and a homeodomain, and plays a key role in the development of eye, brain, and pancreas in vertebrates ... [more ▼]

Pax6 is a well conserved transcription factor that contains two DNA-binding domains, a paired domain and a homeodomain, and plays a key role in the development of eye, brain, and pancreas in vertebrates. The recent identification of the zebrafish sunrise mutant, harboring a mutation in the pax6b homeobox and presenting eye abnormalities but no obvious pancreatic defects, raised a question about the role of pax6b in zebrafish pancreas. We show here that pax6b does play an essential role in pancreatic endocrine cell differentiation, as revealed by the phenotype of a novel zebrafish pax6b null mutant and of embryos injected with pax6b morpholinos. Pax6b-depleted embryos have almost no beta cells, a strongly reduced number of delta cells, and a significant increase of epsilon cells. Through the use of various morpholinos targeting intron-exon junctions, pax6b RNA splicing was perturbed at several sites, leading either to retention of intronic sequences or to deletion of exonic sequences in the pax6b transcript. By this strategy, we show that deletion of the Pax6b homeodomain in zebrafish embryos does not disturb pancreas development, whereas lens formation is strongly affected. These data thus provide the explanation for the lack of pancreatic defects in the sunrise pax6b mutants. In addition, partial reduction of Pax6b function in zebrafish embryos performed by injection of small amounts of pax6b morpholinos caused a clear rise in alpha cell number and in glucagon expression, emphasizing the importance of the fine tuning of the Pax6b level to its biological activity. [less ▲]

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See detailPaying Attention to Language, Replicas and the Role of the Artist in Janet Frame's Living in the Maniototo
Tunca, Daria ULg

in Journal of Postcolonial Writing (2006), 42(1), 32-43

Janet Frame's 1979 novel Living in the Maniototo features a ubiquitous narrator whose multiple personalities are linked by a common interest in creation. This choice of narrative perspective, coupled with ... [more ▼]

Janet Frame's 1979 novel Living in the Maniototo features a ubiquitous narrator whose multiple personalities are linked by a common interest in creation. This choice of narrative perspective, coupled with the characters and events depicted in the book, provides the basis for an exploration of the related concepts of art, language and replicas. By establishing connections between these elements, this article attempts to unveil the dynamics at work in the novel's multi-layered structure and thus shed light on the role of the artist in the narrative and, by extension, on the author's metafictional strategy. [less ▲]

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See detailPayment of a financial obligation from Tebtynis
Macedo, Gabriel ULg

in Bulletin of the American Society of Papyrologists (2013)

Edition, translation and commentary of P. Tebt. 2. 523 descr., a second century Greek documentary papyrus.

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See detailLe pays de Tintin, l'ami de Tchang: la Belgique et sa Communauté française
Defays, Jean-Marc ULg

Scientific conference (1997, December)

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See detailLe pays mosan : un terroir hagiographique et artistique privilégié (VIIIe-XVIe siècle)?
George, Philippe ULg

Scientific conference (2013, July 17)

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See detailLe pays mosan, laboratoire hagiographique impérial ?
George, Philippe ULg

in BOZOKI Edina (Ed.) Hagiographie, idéologie et politique au Moyen Age en Occident (2012)

The Mosan region, hagiographic imperial laboratory? If political and economic conditions particularly favorables for eleventh and twelfth centuries in the old diocese of Liege make the golden age of the ... [more ▼]

The Mosan region, hagiographic imperial laboratory? If political and economic conditions particularly favorables for eleventh and twelfth centuries in the old diocese of Liege make the golden age of the Mosan art (the Meuse river valley), the hagiography is not outdone. Exceptional narrative sources instrumentalize the Mosan saints as powerful protectors in the space of an episcopal principality dependent on the the Holy Roman Empire. The working hypothesis is verified through the strict sense of hagiography : Triumphus sancti Remacli (c. 1071), Passio Agilolfi, Translatio Malmundarium and Miracula sancti Quirini et aliorum (after 1062), in a prolific author Sigebert of Gembloux, and in some scattered texts ((Vita Lamberti of canon Nicolas (c. 1145), relation of the pilgrimage Liege in Santiago ...). [less ▲]

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See detailLe pays
Merenne-Schoumaker, Bernadette ULg; Mérenne, Emile

in La Belgique (1993)

Présentation générale de la Belgique dans un guide de voyage

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See detailLes Pays-Bas : le paradis des aménageurs ? Le cas de la ville de Nimègue
Halleux, Jean-Marie ULg; van der Krabben, Erwin

Conference given outside the academic context (2012)

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See detailLe paysage comme outil d’aménagement des terroirs villageois
Dubiez, Emilien; Vermeulen, Cédric ULg; Tonneau, Jean-Philippe et al

in Bois et Forêts des Tropiques (2013), 315(1), 11-20

The management of fuelwood in periurban areas has become a major concern in Central Africa, where growing demand for fuelwood is rapidly increasing the radius of deforestation around its cities. The ... [more ▼]

The management of fuelwood in periurban areas has become a major concern in Central Africa, where growing demand for fuelwood is rapidly increasing the radius of deforestation around its cities. The village communities within these areas are living in an environment that has become degraded to the point where forest ecosystems and their associated goods and services have almost disappeared. The over-exploitation of these areas demands new thinking on the management of community lands. This paper presents a simple management planning approach for community areas. The article discusses the relevance of a management approach based on landscape as perceived and used by the local populations themselves. The structure of these landscapes has evolved with each community’s pattern of social organization, practices and rules of access to resources, all of which translate into the typology of a local landscape in daily use. This typology has been called upon to strengthen community appropriation and implementation of development concepts. In contrast with the community forestry approach that has developed in Central Africa for several years, this approach is built around a participatory, iterative, step-by-step process that allows endogenous groups to bring their own vision of their landscape into decision- making, to facilitate discussions around village land management issues and to contribute to the rehabilitation of degraded forest areas. [less ▲]

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See detailPaysage dans l’action, paysage en action(s). Développement territorial et processus de construction de projets locaux de paysage en Wallonie (Belgique).
Droeven, Emilie ULg

Doctoral thesis (2010)

The European Landscape Convention acknowledges landscape as a key element of territorial development, management and planning. At the same time there has been a rising public concern about landscape ... [more ▼]

The European Landscape Convention acknowledges landscape as a key element of territorial development, management and planning. At the same time there has been a rising public concern about landscape evolution and a strong social demand for high quality landscape. In scientific and institutional spheres, as well as within civil society, this has resulted in a growing interest for the everyday landscape and an enlargement of landscape concerns to larger spaces. Consequently a diversification of actors, with an interest in the definition and implementation of the landscape policies, has taken place. These evolutions in landscape concerns require land management and planning, thus permitting new action and research perspectives. Considering that landscape projects can favourably respond to these evolutions, this research, conducted with the support of the Standing Conference on Territorial Development of the Walloon Region, investigates the processes of landscape project design on a local scale in Wallonia (Belgium). The aim of the research is to analyze how action is being carried out in the context of landscape use. In order to do so, it develops a framework for characterizing and interpreting these observed processes. The specificity of the research is to examine the practical implementation of on-going landscape action. Based on the empirical analysis of nine case studies, taking place in Wallonia (Nature Parks, Local Action Groups, River Contracts), the research takes the form of an inductive, holistic, transdisciplinary and qualitative approach. The methods of investigation are a literature review, a stakeholder interview (project coordinator) and participant observation. The research emphasizes four dimensions of the landscape projects: rising and conception conditions (through actors and instruments of public action analysis), implemented actions and underlying social representations. Each enables the researcher to define characterization criteria and specific categories. In doing so, the research relates project contributions and limits assuming new landscape management challenges. In conclusion, it reframes the contribution of the landscape issue to regional development and outlines prospects for further action and research. [less ▲]

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See detailPaysage et développement territorial, apports et limites de la démarche de projet local de paysage. Le cas de la Wallonie (Belgique)
Droeven, Emilie ULg

Conference (2011, December 09)

The European Landscape Convention acknowledges landscape as a key element in territorial development, management and planning (assessment of the effects of certain public and private projects on the ... [more ▼]

The European Landscape Convention acknowledges landscape as a key element in territorial development, management and planning (assessment of the effects of certain public and private projects on the environment, re-landscaping, landscape projects design, charters…). Based on a doctoral research investigating the processes of landscape project design on a local scale in Wallonia (Belgium) in Nature Parks, Local Action Groups or River Contracts, the paper tries to analyze how landscape influences territorial practices and issues. It aims also to produce an evaluation on how landscape action transforms territorial governance and decision-making process. In doing so, the paper relates landscape project contributions and limits assuming new landscape management challenges. And finally, it reframes the contribution of the landscape issue to regional and local development. [less ▲]

Detailed reference viewed: 41 (6 ULg)