Rizzanèse (Corse) - Projet de vanne de transit hydrosédimentaire - Réalisation d'un modèle réduit

Report (2008)

Rmd9p controls the processing/stability of mitochondrial mRNAs and its overexpression compensates for a partial deficiency of oxa1p in Saccharomyces cerevisiae.

in Genetics (2007), 175(3), 1105-15

Oxa1p is a key component of the general membrane insertion machinery of eukaryotic respiratory complex subunits encoded by the mitochondrial genome. In this study, we have generated a respiratory ... [more ▼]

Oxa1p is a key component of the general membrane insertion machinery of eukaryotic respiratory complex subunits encoded by the mitochondrial genome. In this study, we have generated a respiratory-deficient mutant, oxa1-E65G-F229S, that contains two substitutions in the predicted intermembrane space domain of Oxa1p. The respiratory deficiency due to this mutation is compensated for by overexpressing RMD9. We show that Rmd9p is an extrinsic membrane protein facing the matrix side of the mitochondrial inner membrane. Its deletion leads to a pleiotropic effect on respiratory complex biogenesis. The steady-state level of all the mitochondrial mRNAs encoding respiratory complex subunits is strongly reduced in the Deltarmd9 mutant, and there is a slight decrease in the accumulation of two RNAs encoding components of the small subunit of the mitochondrial ribosome. Overexpressing RMD9 leads to an increase in the steady-state level of mitochondrial RNAs, and we discuss how this increase could suppress the oxa1 mutations and compensate for the membrane insertion defect of the subunits encoded by these mRNAs. [less ▲]

RMI-Dourbes ionosonde database - Status 2010

Report (2011)

RNA editing of larch mitochondrial tRNA(His) precursors is a prerequisite for processing

in Nucleic Acids Research (1996), 24(16), 3229-3234

Larch mitochondria contain a 'native' tRNA(His) which is absent from angiosperms, Sequence comparisons of genomic DNA and cDNA obtained from unprocessed primary transcripts of the larch mitochondrial gene ... [more ▼]

Larch mitochondria contain a 'native' tRNA(His) which is absent from angiosperms, Sequence comparisons of genomic DNA and cDNA obtained from unprocessed primary transcripts of the larch mitochondrial gene trnH encoding this tRNA revealed three nucleotide discrepancies. These three nucleotide alterations, in the acceptor stem, D stem and anticodon stem respectively, are conversions of genomic cytidines to thymidines in the cDNA (uridines in the tRNA) and thus resemble the RNA editing events observed in nearly all plant mitochondrial mRNAs. Two cases of editing affecting mitochondrial tRNAs from angiosperms have already been described, but we present here the first example of such events in a gymnosperm mitochondrial tRNA. All three editing events correct mismatched C . A base pairs which appear when folding the gene sequence into the standard cloverleaf structure, thereby improving the secondary structure of the tRNA. When incubated with a heterologous potato mitochondrial processing extract, only the edited form of the larch mitochondrial tRNA(His) precursor was efficiently processed in vitro. These data strongly suggest that editing of larch mitochondrial tRNA(His) is a prerequisite for its processing. [less ▲]

RNA silencing in the dermatophyte Microsporum canis

in FEMS Microbiology Letters (2007), 275(1), 38-45

Dermatomycoses caused by Microsporum canis are frequent in domestic animals and easily transmissible to humans. Several proteases secreted by this fungus were identified as potential virulence factors ... [more ▼]

Dermatomycoses caused by Microsporum canis are frequent in domestic animals and easily transmissible to humans. Several proteases secreted by this fungus were identified as potential virulence factors, but the construction of deficient strains is required to investigate their role in the pathogenesis of the disease. Using target genes encoding two of these proteases, a first evaluation of the utility of RNA-mediated silencing as a reverse genetic tool in dermatophytes was carried out. SUB3 and DPPIV, respectively coding for a subtilisin and a dipeptidyl peptidase, were both down-regulated, by means of two plasmid constructs designed to express an RNA hairpin that corresponds to part of their respective sequence. The degree of attenuation was evaluated by enzymatic assay of the transformants culture supernatants, and by real-time reverse transcriptase-polymerase chain reaction. Enzymatic activities and expression levels varied from less than 5% to 100% of that of control transformants obtained with plasmid without hairpin inserts. Inhibition was globally more efficient for SUB3 than for DPPIV. These results show that RNA silencing can be used for functional genomics in M. canis, and particularly to circumvent the limits and technical difficulties of conventional disruption methods. [less ▲]

Road Pricing as an Impetus for Environment-Friendly Travel Behavior: Results from Stated Adaptation Experiment

in Proceedings of the 88th Annual Meeting of the Transportation Research Board (DVD-ROM) (2009)

An important policy instrument for governments to modify travel behavior and manage the increasing travel demand is the introduction of a congestion pricing system. In this study, the influence of a ... [more ▼]

An important policy instrument for governments to modify travel behavior and manage the increasing travel demand is the introduction of a congestion pricing system. In this study, the influence of a detailed classification of activities is examined to assess likely traveler response to congestion pricing scenarios. Despite the fact that most studies do not differentiate between activity categories, the value of time and in general the space-time properties and constraints of different types of activities vary widely. For this reason, it is of importance to provide sufficient detail and sensitivity in assessing the impact of congestion pricing scenarios. In addition, a first assessment of possible multi-faceted adaptation patterns of travelers is presented. For these purposes, a stated adaptation study was conducted in Flanders (Dutch speaking region of Belgium). The experiment was conducted through an interactive stated adaptation survey. In the stated adaptation experiment respondents could indicate their stated responses to the congestion pricing scenario. The most prevalent conclusion is that the activity type significantly predetermines the willingness to express a more environmental-friendly behavior (i.e. reducing the number of trips, reducing the total distance traveled, switching to more environmental-friendly modes). Also, the willingness to show ecological activity-travel behavior (e.g. carpooling and using public transport) in a non-pricing situation is a major differentiator of future behavior in a congestion pricing scenario. [less ▲]

The ROB-IIV GPS Network on Etna. First Results

in Flick, J. (Ed.) Cahiers du Centre Européen de Géodynamique et de Séismologie - Volume 8 (1993)

Robert Cavenaile (1918-2007)

in Capasso, Mario (Ed.) Hermae. Scholars and Scholarship in Papyrology II (2010)

Life and works of Robert Cavenaile (Wasmes, Belgium, 1918-Liège, Belgium, 2007), Belgian papyrologist and philologist, author of the Corpus Papyrorum Latinarum

Robert CRYER, Tamara HERVEY, Bal SOKHI-BULLEY et Alexandra BOHM, Research Methodologies in EU and International Law, Oxford, Hart, 2011, 143 p.

in Revue Trimestrielle de Droit Européen (2011), 47(4),

Cet ouvrage propose, principalement à l’attention des jeunes doctorants mais son thème intéressera tout auteur de doctrine, une synthèse des différentes méthodologies de la recherche (heuristique) et de ... [more ▼]

Cet ouvrage propose, principalement à l’attention des jeunes doctorants mais son thème intéressera tout auteur de doctrine, une synthèse des différentes méthodologies de la recherche (heuristique) et de la critique (herméneutique) juridique dans le domaine du droit européen et du droit international. [less ▲]

Robert GUIETTE, Poésies complètes, édition et introduction par Gérald Purnelle, préface de Philippe Jones

Book published by Le Taillis Pré (2011)