Over stromen en watervallen : curricua die niet discrimineren
; ; Baye, Ariane
in Nicaise, Julien; Desmedt, E. (Eds.) Gelijke kansen op school : het kan. Zestein sporen voor praktijk en beleid (2008)Detailed reference viewed: 18 (3 ULg)
An Overall Magnetic $t$-$\omega$ Formulation without Cuts
; Dular, Patrick ; Geuzaine, Christophe et al
in Proceedings of the 8th Conference on Electromagnetic Field Computation, CEFC 1998 (1998)Detailed reference viewed: 51 (1 ULg)
Overall properties of hot, massive stars in the X-ray domain
(2011, July 01)
Despite the absence of large surveys, the recent X-ray observatories provide X-ray data for hundreds of massive stars (294 OB stars detected in the 2XMM catalog, 129 OB stars detected in the Chandra ... [more ▼]
Despite the absence of large surveys, the recent X-ray observatories provide X-ray data for hundreds of massive stars (294 OB stars detected in the 2XMM catalog, 129 OB stars detected in the Chandra Carina Complex Project). Analyzing medium-resolution spectra led to new results on the relationship between the X-ray luminosity and the bolometric luminosity, as well as on the typical properties (plasma temperature, variability) of these objects. [less ▲]Detailed reference viewed: 8 (0 ULg)
Overall Safety and Treatment Duration in Lenalidomide (LEN)-, Thalidomide (THAL)-, and Bortezomib (BORT)-Treated Patients within the European Post-Approval Safety Study (EU PASS) of Relapsed/Refractory Multiple Myeloma (RRMM)
; ; et al
Poster (2012)Detailed reference viewed: 8 (0 ULg)
Overcoming problems with adherence to osteoporosis medication.
Rabenda, Véronique ; Reginster, Jean-Yves
in Expert review of pharmacoeconomics & outcomes research (2010), 10(6), 677-89
Nonadherence to pharmacological treatment in osteoporosis is a well-recognized problem. As in other chronic diseases, adherence to osteoporosis treatment is poor, resulting in enormous burden on patients ... [more ▼]
Nonadherence to pharmacological treatment in osteoporosis is a well-recognized problem. As in other chronic diseases, adherence to osteoporosis treatment is poor, resulting in enormous burden on patients and healthcare resources. Most importantly, low adherence rates consistently result in increased rates of fractures. However, it seems that efforts to evaluate and improve rates of both compliance and persistence are increasing. The extension of dosing intervals may be an element, among others, allowing improvements in therapeutic adherence. Improved patient education, enhancing healthcare provider-patients interaction, taking into account patient's preferences and involving them in treatment decisions may improve adherence. [less ▲]Detailed reference viewed: 17 (2 ULg)
Overestimation of the 25OHD serum concentration with the automated IDS EIA kit.
Cavalier, Etienne ; ; et al
in Journal of Bone and Mineral Research (2011), 26(2), 434-6
We have recently observed an increasing number of patients presenting very high serum levels of 25-hydroxyvitamin D [25(OH)D] (> 150 ng/mL), which, in all cases, had been measured with the IDS EIA kit ... [more ▼]
We have recently observed an increasing number of patients presenting very high serum levels of 25-hydroxyvitamin D [25(OH)D] (> 150 ng/mL), which, in all cases, had been measured with the IDS EIA kit adapted on different "open" automated platforms. We performed a comparison between the IDS EIA kit adapted on two different "open"automated platforms and the DiaSorin RIA. We found a systematic bias (higher levels with the IDS EIA kit) for concentrations more than 50-60 ng/mL that was less obvious when the IDS EIA was used in its manual procedure. We thus suggest to use the IDS EIA kit in its manual procedure rather than to adapt it on an automated platform, and to interpret cautiously a 25(OH)D greater than 100 ng/mL with this kit. [less ▲]Detailed reference viewed: 39 (1 ULg)
Overexpression and function of the CIPAR1 gene in a rat prostatic cell line.
Scientific conference (1997, November 29)
In prostate cancer therapy, as the components of the cell death pathway play an important role, it is conceivable that targeted transfer of apoptosis inducer genes into prostatic cells could be useful. A ... [more ▼]
In prostate cancer therapy, as the components of the cell death pathway play an important role, it is conceivable that targeted transfer of apoptosis inducer genes into prostatic cells could be useful. A new gene, CIPAR1 (Castration Induced Prostatic Apoptosis Related gene 1) was identified by differential screening for genes that were upregulated when rat prostates were induced to undergo apoptosis. To define the role of CIPAR1 in the apoptosis pathway, we have therefore investigated its ability to induce apoptosis in the Mat LyLu cells. The CIPAR1 gene was transiently overexpressed in these cells. The rate of apoptosis in the transfected cells was estimated with the help of the following tests: qualitative methods (DNA ladder, TUNEL on slide) and also quantitative methods using flow cytolmetric analysis. Induction of apoptosis was higher in the pcDNA3-CIPAR1 transfected cells than in controls. The establishment of a stable cell line that contains the gene CIPAR1 under the control of an inducible promoter would be an useful model to study the role of CIPAR1 in prostatic cell death. [less ▲]Detailed reference viewed: 19 (1 ULg)
Overexpression of a novel member of the mitochondrial carrier family rescues defects in both DNA and RNA metabolism in yeast mitochondria.
; ; et al
in Molecular & General Genetics [=MGG] (1995), 246
The PIF1 and MRS2 gene products have previously been shown to be essential for mitochondrial DNA maintenance at elevated temperatures and mitochondrial group II intron splicing, respectively, in the yeast ... [more ▼]
The PIF1 and MRS2 gene products have previously been shown to be essential for mitochondrial DNA maintenance at elevated temperatures and mitochondrial group II intron splicing, respectively, in the yeast Saccharomyces cerevisiae. A multicopy suppressor capable of rescuing the respiratory deficient phenotype associated with null alleles of either gene has been isolated. This suppressor is a nuclear gene that was called RIM2/MRS12. The RIM2/MRS12 gene encodes a predicted protein of 377 amino acids that is essential for mitochondrial DNA metabolism and proper cell growth. Inactivation of this gene causes the total loss of mitochondrial DNA and, compared to wild-type rhoo controls, a slow-growth phenotype on media containing glucose. Analysis of the RIM2/MRS12 protein sequence suggests that RIM2/MRS12 encodes a novel member of the mitochondrial carrier family. In particular, a typical triplicate structure, where each repeat consists of two putative transmembrane segments separated by a hydrophilic loop, can be deduced from amino acid sequence comparisons and the hydropathy profile of RIM2/MRS12. Antibodies directed against the aminoterminus of RIM2/MRS12 detect this protein in mitochondria. The function of the RIM2/MRS12 protein and the substrates it might transport are discussed. [less ▲]Detailed reference viewed: 14 (1 ULg)
Overexpression of airway CD39 in transgenic mice enhances lipopolysaccharide-induced inflammation.
Theatre, Emilie ; de Leval, Laurence ; Bettendorff, Lucien et al
in Purinergic Signalling (2008), 4Detailed reference viewed: 26 (9 ULg)
Overexpression of CD39 in mouse airways promotes bacteria induced inflammation
Theatre, Emilie ; ; et al
in Journal of Immunology (2012), 189(4), 1966-1974
In airways, the ecto-nucleoside triphosphate diphosphohydrolase CD39 plays a central role in the regulation of physiological mucosal nucleotide concentrations and likely contributes to the control of ... [more ▼]
In airways, the ecto-nucleoside triphosphate diphosphohydrolase CD39 plays a central role in the regulation of physiological mucosal nucleotide concentrations and likely contributes to the control of inflammation because accelerated ATP metabolism occurs in chronic inflammatory lung diseases.We sought to determine whether constant elevated CD39 activity in lung epithelia is sufficient to cause inflammation and whether this affects the response to acute LPS or Pseudomonas aeruginosa exposure. We generated transgenic mice overexpressing human CD39 under the control of the airway-specific Clara cell 10-kDa protein gene promoter. Transgenic mice did not develop any spontaneous lung inflammation. However, intratracheal instillation of LPS resulted in accelerated recruitment of neutrophils to the airways of transgenic mice. Macrophage clearance was delayed, and the amounts of CD8+ T and B cells were augmented. Increased levels of keratinocyte chemoattractant, IL-6, and RANTES were produced in transgenic lungs. Similarly, higher numbers of neutrophils and macrophages were found in the lungs of transgenic mice infected with P. aeruginosa, which correlated with improved bacteria clearance. The transgenic phenotype was partially and differentially restored by coinstillation of P2X1 or P2X7 receptor antagonists or of caffeine with LPS. Thus, a chronic increase of epithelial CD39 expression and activity promotes airway inflammation in response to bacterial challenge by enhancing P1 and P2 receptor activation. [less ▲]Detailed reference viewed: 26 (8 ULg)
Overexpression of CD9 in human breast cancer cells promotes the development of bone metastases.
; Bellahcene, Akeila ; et al
in Anticancer Research (2012), 32(12), 5211-20
BACKGROUND: Bone is a preferred target for circulating metastatic breast cancer cells. We found that the CD9 protein was up-regulated in the B02 osteotropic cell line, derived from the aggressive parental ... [more ▼]
BACKGROUND: Bone is a preferred target for circulating metastatic breast cancer cells. We found that the CD9 protein was up-regulated in the B02 osteotropic cell line, derived from the aggressive parental MDA-MB-231 breast cancer cell line. Here, we investigated the putative relationship between CD9 expression and the osteotropic phenotype. MATERIALS AND METHODS: Overexpression of CD9 was analyzed by immunoblotting in different cell lines. Immunohistochemistry was used to assess CD9 expression in primary tumors and metastatic lesions. In vivo experiments were conducted in mice using a monoclonal antibody against CD9. RESULTS: CD9 overexpression was confirmed in osteotropic cells. CD9 was significantly overexpressed in bone metastases versus primary tumors and visceral metastatic lesions. Finally, in vivo experiments showed that an antibody against CD9 delays homing of B02 cells in bone marrow, slowing down bone destruction. CONCLUSION: Our study reveals a potential implication of CD9 in the formation of bony metastases from breast cancer cells. [less ▲]Detailed reference viewed: 21 (3 ULg)
Overexpression of GRP94 in breast cancer cells resistant to oxidative stress promotes high levels of cancer cell proliferation and migration: implications for tumor recurrence.
; ; Guénin, Samuel et al
in Free Radical Biology & Medicine (2012), 52(6), 993-1002
Targeting the altered redox status of cancer cells is emerging as an interesting approach to potentiate chemotherapy. However, to maximize the effectiveness of this strategy and define the correct ... [more ▼]
Targeting the altered redox status of cancer cells is emerging as an interesting approach to potentiate chemotherapy. However, to maximize the effectiveness of this strategy and define the correct chemotherapeutic associations, it is important to understand the biological consequences of chronically exposing cancer cells to reactive oxygen species (ROS). Using an H(2)O(2)-generating system, we selected a ROS-resistant MCF-7 breast cancer cell line, namely Resox cells. By exploring different survival pathways that are usually induced during oxidative stress, we identified a constitutive overexpression of the endoplasmic reticulum chaperone, GRP94, in these cells, whereas levels of its cytoplasmic homolog HSP90, or GRP78, were not modified. This overexpression was not mediated by constitutive unfolded protein response (UPR) activation. The increase in GRP94 is tightly linked to an increase in cell proliferation and migration capacities, as shown by GRP94-silencing experiments. Interestingly, we also observed that GRP94 silencing inhibits migration and proliferation of the highly aggressive MDA-MB-231 cells. By immunohistochemistry, we showed that GRP94 expression was higher in recurrent human breast cancers than in their paired primary neoplasias. Similar to the situation in the Resox cells, this increase was not associated with an increase in UPR activation in recurrent tumors. In conclusion, this study suggests that GRP94 overexpression may be a hallmark of aggressiveness and recurrence in breast cancers. [less ▲]Detailed reference viewed: 30 (6 ULg)
Overexpression of non-symbiotic hemoglobin 2 in Arabidopsis thaliana leads to increased oil synthesis in seeds
Conference (2005)Detailed reference viewed: 8 (1 ULg)
Overexpression of the 67-kD laminin receptor correlates with tumor progression in human prostate cancer
Waltregny, David ; de Leval, Laurence ; et al
in Proceedings of the American Association for Cancer Research (1997), 38Detailed reference viewed: 5 (0 ULg)
Overexpression of the 67-kD laminin receptor correlates with tumour progression in human colorectal carcinoma.
; ; et al
in Journal of Pathology (The) (1996), 179(4), 376-80
The high affinity 67-kD laminin receptor (67LR) is a cell surface protein whose expression is increased in a number of human carcinoma models. To date, 67LR expression in colorectal carcinomas has been ... [more ▼]
The high affinity 67-kD laminin receptor (67LR) is a cell surface protein whose expression is increased in a number of human carcinoma models. To date, 67LR expression in colorectal carcinomas has been examined in a small number of cases. 67LR expression has been immunohistochemically analysed in a large series of human colorectal neoplasms, using the MLuC5 monoclonal antibody. The study included 59 samples of non-neoplastic mucosa, 45 polyps (11 hyperplastic, 34 adenomas), 196 carcinomas, and lymph node metastases of 87 carcinomas. Epithelial cells of normal mucosa and hyperplastic polyps were negative or showed weak positivity in the paranuclear and apical areas of the cytoplasm. In adenomas and carcinomas, the staining was stronger, with a membranous or cytoplasmic pattern. The expression of 67LR correlated significantly with the progression from normal mucosa (22 per cent) to adenoma (44 per cent), carcinoma (61 per cent), and lymph node metastasis (75 per cent) (P < 0.0001). Expression of the laminin receptor showed a tendency to be more frequently positive in advanced stage (III+IV; 67 (III+IV; 67 per cent) when compared with early stage (I+II) carcinomas (54 per cent). The difference, however, was not statistically significant (P = 0.058). In addition, 14 out of 28 (50 per cent) primary carcinomas without 67LR expression became positive in lymph node metastases, while most (86 per cent) of the MLuC5-positive primary carcinomas were also immunoreactive in metastases. In conclusion, these results indicate that 67LR is up-regulated in the progression of human colorectal carcinomas and may play a role in the local and metastatic progression of these tumours. [less ▲]Detailed reference viewed: 9 (3 ULg)
Overexpression of the 67-kDa laminin receptor correlates with tumor progression in human prostate cancer
Waltregny, David ; de Leval, Laurence ; et al
in Clinical & Experimental Metastasis (1996, March), 14(Suppl. 1), 1996Detailed reference viewed: 10 (0 ULg)
The overexpression of the c-erbB2 mRNA in the tumor-derived BT-474 cell line is correlated with the presence of trans-acting factors with elevated transcriptional activity.
Pasleau, Françoise ; ; et al
Poster (1994, June)Detailed reference viewed: 4 (0 ULg)
Overexpression of the homeobox gene HOXC8 in human prostate cancer correlates with loss of tumor differentiation
Waltregny, David ; ; et al
in Prostate (2002), 50(3), 162-169
BACKGROUND. Homeobox (HOX)-containing proteins have been identified as regulators controlling the coordinated expression of genes involved in development and differentiation. Recent data also suggest a ... [more ▼]
BACKGROUND. Homeobox (HOX)-containing proteins have been identified as regulators controlling the coordinated expression of genes involved in development and differentiation. Recent data also suggest a possible involvement of HOX genes in malignant transformation and/or progression. We have previously shown that HOXC8 expression was selectively turned on in human cervix cancer cells compared with normal keratinocytes, suggesting that HOXC8 may be involved in the process leading to the transformation of cervix keratinocytes [Alami et al.: Biochem Biophys Res Commun 257:738-745,1999]. METHODS. RT-PCR and in situ hybridization experiments were performed to investigate the expression and cell type localization of HOXC8 transcripts in human prostate cancer cell lines and tissues. In situ hybridization was performed with the use of an HOXC8 anti-sense digoxigenin-labeled probe to investigate HOXC8 mRNA expression in 27 prostate cancer tissue specimens. RESULTS. Out of the three human prostate cancer cell lines tested, DU-145 and PC3 but not LNCaP cells expressed detectable amount of HOXC8 transcripts. Results from in situ hybridization experiments demonstrated that HOXC8 gene was expressed mainly in malignant epithelial cells. Furthermore, the staining intensity in epithelial cells was significantly increased in high Gleason score carcinomas (scores 7-9, n = 12; labeling intensity 2 + to 3 +) compared with the one observed in low and intermediate Gleason score tumors (scores 3-6, n = 15; labeling intensity 0 and 1 +) (ANOVA test, P < 0.0001). CONCLUSIONS. Our data showing that HOXC8 overexpression is associated with the loss of tumor differentiation in human prostate cancer suggests that HOXC8 may play a role in the acquisition of the invasive and metastatic phenotype of this malignancy. (C) 2002 Wiley-Liss, Inc. [less ▲]Detailed reference viewed: 37 (3 ULg)
Overexpression of the platelet P2X1 ion channel in transgenic mice generates a novel prothrombotic phenotype.
Oury, Cécile ; ; et al
in Journal of Thrombosis and Haemostasis (2003)Detailed reference viewed: 19 (1 ULg)