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See detailNew models for analyzing mast cell functions in vivo.
Marichal, Thomas ULg; Reber, Laurent L; Galli, Stephen J.

in Trends in Immunology (2012), 33(12), 613-25

In addition to their well-accepted role as critical effector cells in anaphylaxis and other acute IgE-mediated allergic reactions, mast cells (MCs) have been implicated in a wide variety of processes that ... [more ▼]

In addition to their well-accepted role as critical effector cells in anaphylaxis and other acute IgE-mediated allergic reactions, mast cells (MCs) have been implicated in a wide variety of processes that contribute to disease or help to maintain health. Although some of these roles were first suggested by analyses of MC products or functions in vitro, it is critical to determine whether, and under which circumstances, such potential roles actually can be performed by MCs in vivo. This review discusses recent advances in the development and analysis of mouse models to investigate the roles of MCs and MC-associated products during biological responses in vivo, and comments on some of the similarities and differences in the results obtained with these newer versus older models of MC deficiency. [less ▲]

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See detailNew monomers tailored for direct electrografting onto carbon in water
Cecius, Michaël ULg; Jérôme, Robert ULg; Jérôme, Christine ULg

in Macromolecular Rapid Communications (2007), 28(8), 948-954

Electrografting, which is an electropolymerization technique dedicated to the chemisorption of polyacrylates onto conductive surfaces, was actively studied in anhydrous organic media until now. This ... [more ▼]

Electrografting, which is an electropolymerization technique dedicated to the chemisorption of polyacrylates onto conductive surfaces, was actively studied in anhydrous organic media until now. This communication aims at extending this technique to aqueous media for the grafting of polyacrylates onto carbon. The key criterion for the successful electrografting under these not very demanding experimental conditions (water vs. anhydrous organic solvents) is the use of amphiphilic acrylic monomers. The impact of the chemical structure of these new monomers on the coating properties was investigated. Composition of the coatings was analyzed by FT-IR ATR and XPS, whereas film thickness and morphology were analyzed by ellipsometry and AFM, respectively. [less ▲]

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See detailA new MPC scheme for damping wide-area electromechanical oscillations in power systems
Wang, Da ULg; Glavic, Mevludin; Wehenkel, Louis ULg

in the 2011 IEEE PES PowerTech (2011, June)

This paper introduces a new Model Predictive Control (MPC) scheme to damp wide-area electromechanical oscillations. The proposed MPC controller, based on a linearized discrete-time state space model ... [more ▼]

This paper introduces a new Model Predictive Control (MPC) scheme to damp wide-area electromechanical oscillations. The proposed MPC controller, based on a linearized discrete-time state space model, calculates the optimal input sequence for local damping controllers over a chosen time horizon by solving a quadratic programming problem. Local controllers considered include: Power Systems Stabilizers (PSSs), Thyristor Controlled Series Compensators (TCSCs) and Static Var Compensators (SVCs). The MPC scheme is realized and tested first in ideal conditions (complete state observability and controllability, neglecting communication and computing delays). Next, the effects of state-estimation errors, computation and communication delays, and of the number and type of available local damping controllers are studied in order to assess the versatility of this scheme. Realistic simulations are carried out using a 16 generators, 70 bus test system. [less ▲]

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See detailNew multi-station and multi-decadal trend data on precipitable water. Recipe to match FTIR retrievals from NDACC long-time records to radio sondes within 1mm accuracy/precision
Sussmann, Ralf; Borsdorf, Tobias; Rettinger, M. et al

in Geophysical Research Abstracts (2009), 11

We present an original optimum strategy for retrieval of precipitable water from routine ground-based midinfrared FTS measurements performed at a number globally distributed stations within the NDACC ... [more ▼]

We present an original optimum strategy for retrieval of precipitable water from routine ground-based midinfrared FTS measurements performed at a number globally distributed stations within the NDACC network. The strategy utilizes FTIR retrievals which are set in a way to match standard radio sonde operations. Thereby, an unprecedented accuracy and precision for measurements of precipitable water can be demonstrated: the correlation between Zugspitze FTIR water vapor columns from a 3 months measurement campaign with total columns derived from coincident radio sondes shows a regression coefficient of R = 0.988, a bias of 0.05 mm, a standard deviation of 0.28 mm, an intercept of 0.01 mm, and a slope of 1.01. This appears to be even better than what can be achieved with state-of-the-art micro wave techniques, see e.g., Morland et al. (2006, Fig. 9 therein). Our approach is based upon a careful selection of spectral micro windows, comprising a set of both weak and strong water vapor absorption lines between 839.4 – 840.6 cm-1, 849.0 – 850.2 cm-1, and 852.0 – 853.1 cm-1, which is not contaminated by interfering absorptions of any other trace gases. From existing spectroscopic line lists, a careful selection of the best available parameter set was performed, leading to nearly perfect spectral fits without significant forward model parameter errors. To set up the FTIR water vapor profile inversion, a set of FTIR measurements and coincident radio sondes has been utilized. To eliminate/minimize mismatch in time and space, the Tobin best estimate of the state of the atmosphere principle has been applied to the radio sondes. This concept uses pairs of radio sondes launched with a 1-hour separation, and derives the gradient from the two radio sonde measurements, in order to construct a virtual PTU profile for a certain time and location. Coincident FTIR measurements of water vapor columns (two hour mean values) have then been matched to the water columns obtained by integrating the best-estimate radio sonde profiles. This match was achieved via investigating the quality of the correlation plots between the columns derived from the radio sondes and the FTIR retrievals, and iteratively tuning the regularization strength of the FTIR retrieval. The FTIR regularization matrix is based on a Tikhonov operator which allows for empirical tuning of the regularization strength via one parameter. The figures of merit for the iterative tuning have been the slope, the intercept, and the regression coefficient of the correlation. By this way an optimum retrieval setting could be found, guaranteeing a response of the FTIR retrievals to true water vapor changes, which is matched to the radio sonde operation. As first examples for utilizing this approach to derive long-term trends of precipitable water from NDACC type long-term FTIR measurements, we present trends from two time series. I.e., one retrieved from continuous FTIR measurements at the NDACC Primary Station Zugspitze, Germany (47.42 °N, 10.98 °E, 2964 m a.s.l.), which covers the time span 1995-2009, and one from the International Scientific Station of the Jungfraujoch (ISSJ, 46.5°N, 8.0°E, 3580m a.s.l., Swiss Alps), covering the time span 1984 – 2009. A detailed trend analysis of both series via the bootstrap method will be presented. In ongoing work we apply this optimum retrieval approach to historical long-time measurement series of further selected FTIR stations of the NDACC network. Thereby we will obtain unprecedented new climate data via long term trends of precipitable water at a set of globally distributed locations. [less ▲]

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See detailA new multifractal formalism based on wavelet leaders : detection of non concave and non increasing spectra (Part I)
Esser, Céline ULg; Kleyntssens, Thomas ULg; Nicolay, Samuel ULg et al

Conference (2014, March 25)

Multifractal analysis is concerned with the study of very irregular signals. For such functions, the pointwise regularity may change widely from a point to another. Therefore, it is more interesting to ... [more ▼]

Multifractal analysis is concerned with the study of very irregular signals. For such functions, the pointwise regularity may change widely from a point to another. Therefore, it is more interesting to determine the spectrum of singularities of the signal, which is the Hausdorff dimension of the set of points which have the same Hölder exponent. The spectrum of singularities of many mathematical functions can be determined directly from its definition. However, for many real-life signals, the numerical determination of their Hölder regularity is not feasible. Therefore, one cannot expect to have a direct access to their spectrum of singularities and one has to find an indirect way to compute it. A multifractal formalism is a formula which is expected to yield the spectrum of singularities from quantities which are numerically computable. Several multifractal formalisms based on the wavelet coefficients of a signal have been proposed to estimate its spectrum. The most widespread of these formulas is the so-called thermodynamic multifractal formalism, based on the Frish-Parisi conjecture. This formalism presents two drawbacks: it can hold only for spectra that are concave and it can yield only the increasing part of the spectrum. This first problem can be avoided using Snu spaces. The second one can be avoided using a formalism based on wavelet leaders of the signal. In this talk, we propose a new multifractal formalism, based on a generalization of the Snu spaces using wavelet leaders. It allows to detect non concave and non increasing spectra. An implementation of this method is presented in the talk "A new multifractal formalism based on wavelet leaders: detection of non concave and non increasing spectra (Part II)" of T. Kleyntssens. [less ▲]

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See detailA new multifractal formalism based on wavelet leaders: detection of non concave and non increasing spectra (Part II)
Kleyntssens, Thomas ULg; Esser, Céline ULg; Nicolay, Samuel ULg

Conference (2014, March 25)

This talk follows "A new multifractal formalism based on wavelet leaders: detection of non concave and non increasing spectra (Part I)" given by Céline Esser. For real-life signals, it is impossible to ... [more ▼]

This talk follows "A new multifractal formalism based on wavelet leaders: detection of non concave and non increasing spectra (Part I)" given by Céline Esser. For real-life signals, it is impossible to compute the spectrum of singularities by using its definition. A multifractal formalism is used to approximate this spectrum. We present a new multifractal formalism for non concave and non increasing spectra based on wavelet leaders. In this talk, an implementation of this formalism is given and several numerical examples are presented. [less ▲]

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See detailA new murine model of osteoblastic/osteolytic lesions from human androgen-resistant prostate cancer.
Fradet, Anais; Sorel, Helene; Depalle, Baptiste et al

in PloS one (2013), 8(9), 75092

BACKGROUND: Up to 80% of patients dying from prostate carcinoma have developed bone metastases that are incurable. Castration is commonly used to treat prostate cancer. Although the disease initially ... [more ▼]

BACKGROUND: Up to 80% of patients dying from prostate carcinoma have developed bone metastases that are incurable. Castration is commonly used to treat prostate cancer. Although the disease initially responds to androgen blockade strategies, it often becomes castration-resistant (CRPC for Castration Resistant Prostate Cancer). Most of the murine models of mixed lesions derived from prostate cancer cells are androgen sensitive. Thus, we established a new model of CRPC (androgen receptor (AR) negative) that causes mixed lesions in bone. METHODS: PC3 and its derived new cell clone PC3c cells were directly injected into the tibiae of SCID male mice. Tumor growth was analyzed by radiography and histology. Direct effects of conditioned medium of both cell lines were tested on osteoclasts, osteoblasts and osteocytes. RESULTS: We found that PC3c cells induced mixed lesions 10 weeks after intratibial injection. In vitro, PC3c conditioned medium was able to stimulate tartrate resistant acid phosphatase (TRAP)-positive osteoclasts. Osteoprotegerin (OPG) and endothelin-1 (ET1) were highly expressed by PC3c while dikkopf-1 (DKK1) expression was decreased. Finally, PC3c highly expressed bone associated markers osteopontin (OPN), Runx2, alkaline phosphatase (ALP), bone sialoprotein (BSP) and produced mineralized matrix in vitro in osteogenic conditions. CONCLUSIONS: We have established a new CRPC cell line as a useful system for modeling human metastatic prostate cancer which presents the mixed phenotype of bone metastases that is commonly observed in prostate cancer patients with advanced disease. This model will help to understand androgen-independent mechanisms involved in the progression of prostate cancer in bone and provides a preclinical model for testing the effects of new treatments for bone metastases. [less ▲]

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See detailNew mutations in ADC-type beta-lactamases from Acinetobacter spp. affect cefoxitin and ceftazidime hydrolysis.
Perez, Astrid; Perez-Llarena, Francisco Jose; Garcia, Patricia et al

in The Journal of antimicrobial chemotherapy (2014)

OBJECTIVES: Two natural variants of ADC-type beta-lactamases of Acinetobacter spp., ADC-1 and ADC-5, differ by nine mutations in their protein sequence. ADC-5 hydrolyses cefoxitin better than ADC-1 and ... [more ▼]

OBJECTIVES: Two natural variants of ADC-type beta-lactamases of Acinetobacter spp., ADC-1 and ADC-5, differ by nine mutations in their protein sequence. ADC-5 hydrolyses cefoxitin better than ADC-1 and the opposite is true for ceftazidime. We produced single and combined mutations in ADC-5 and characterized the variants microbiologically and biochemically to determine which amino acid residues are involved in the hydrolysis of beta-lactam antibiotics in this family of beta-lactamases. METHODS: Site-directed mutagenesis, with blaADC-5 as a source of DNA, was used to generate nine single mutated and three combined mutated enzymes. The proteins (wild-type and derivatives) were then expressed in isogenic conditions in Escherichia coli. MICs of beta-lactams were determined using Etest strips. ADC-1, ADC-5, ADC-5-P167S and ADC-5-P167S/D242G/Q163K/G342R were also purified and the kinetic parameters determined for ceftazidime, cefoxitin, cefalotin and ampicillin. RESULTS: Single mutations did not significantly convert the hydrolysis spectrum of the ADC-5 enzyme into that of the ADC-1 enzyme, although among all studied mutants only the quadruple mutant (ADC-5-P167S/D242G/Q163K/G342R) displayed microbiological and biochemical properties consistent with those of ADC-1. CONCLUSIONS: Although some single mutations are known to affect cefepime hydrolysis in ADC-type beta-lactamases, little is known about ceftazidime and cefoxitin hydrolysis in this family of beta-lactamases. Hydrolysis of these antibiotics appears to be positively and negatively affected, respectively, by the Q163K, P167S, D242G and G342R amino acid replacements. [less ▲]

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See detailNew nanocomposite materials based on plasticized poly(L-lactide) and organo-modified montmorillonites: thermal and morphological study
Paul, Marie-Amélie; Degée, Philippe; Henrist, Catherine ULg et al

in Polymer (2003), 44(2), 443-450

Plasticized poly(L-lactide) (PLA) based nanocomposites were prepared by melt blending of the matrix with 20 wt% of poly(ethyleneglycol) 1000 (PEG 1000) and different amounts of montmorillonite, organo ... [more ▼]

Plasticized poly(L-lactide) (PLA) based nanocomposites were prepared by melt blending of the matrix with 20 wt% of poly(ethyleneglycol) 1000 (PEG 1000) and different amounts of montmorillonite, organo-modified or not. The intercalation of the polymer chains between the aluminosilicates layers and morphological structure of the filled PLAs were analysed by wide-angle X-ray scattering (WAXS). Thermogravimetric analyses (TGA) and differential scanning calorimetry (DSC) were performed to study the thermal behaviour of the prepared composites. At constant filler level, it appears that from all the clays studied, the montmorillonite organo-modified by bis-(2-hydroxyethyl)methyl (hydrogenated tallowalkyl) ammonium cations brings the greater effect in terms of thermal stability. Increasing the amount of clay allows to delay the onset of thermal degradation of the plasticized polymer matrix. It was also pointed out, by WAXS and DSC analyses, that it exists a real competition between PEG 1000 and PLA for the intercalation into the interlayer spacing of the clay. (C) 2002 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailNew nanostructured materials based on fullerene and biodegradable polyesters
Stoilova, Olya; Jérôme, Christine ULg; Detrembleur, Christophe ULg et al

in Chemistry of Materials (2006), 18(20), 4917-4923

Star-shaped poly(epsilon-caprolactone) (PCL) with a fullerene (C-60) core, C-60[N(CH2)(12)OPCLOH](x), was successfully synthesized by reaction of azide-terminated PCL with C-60. Both the experimental ... [more ▼]

Star-shaped poly(epsilon-caprolactone) (PCL) with a fullerene (C-60) core, C-60[N(CH2)(12)OPCLOH](x), was successfully synthesized by reaction of azide-terminated PCL with C-60. Both the experimental conditions and the stoichiometry were optimized, such that an average of six PCL chains was grafted per fullerene core. The molecular weight of the polyester chains directly controlled the length of the arms of the star-shaped polymers. Singlet oxygen was generated on irradiation of the C-60[N(CH2)(12)OPCLOH] x nanohybrids. These C-60[N(CH2)(12)OPCLOH](x) nanohybrids were then processed in two kinds of nanomaterials. First, they were encapsulated within the core of micelles formed by biocompatible amphiphilic block copolymers. In water, the particle size distribution of these nanoparticles was narrow, and their diameter was in the range of 100 to 200 nm. Second, C-60-containing micro-/nanosized polymer fibers were prepared, for the first time, by electrospinning. The average diameter of the fibers was varied by tuning the PCL/C-60[N(CH2)(12)OPCLOH](x) weight ratio. Grafting of polyester chains onto C-60 is thus a suitable strategy for producing easily processable C-60 and attractive building blocks for incorporation of C-60 in nanomaterials. [less ▲]

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See detailNew national and regional bryophyte records, 12
Blockeel, T. L.; Chlebicko, A.; Hajkova, P. et al

in Journal of Bryology (2006), 28(1), 68-70

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See detailA new natural hGH variant--17.5 kd--produced by alternative splicing. An additional consensus sequence which might play a role in branchpoint selection
Lecomte, C. M.; Renard, A.; Martial, Joseph ULg

in Nucleic Acids Research (1987), 15(16), 6331-48

From a human pituitary cDNA library, we have cloned 3 distinct human growth hormone (hGH) cDNAs, coding respectively for the 22 K hGH, the 20 K variant, and a yet unknown 17.5 K variant. S1 mapping ... [more ▼]

From a human pituitary cDNA library, we have cloned 3 distinct human growth hormone (hGH) cDNAs, coding respectively for the 22 K hGH, the 20 K variant, and a yet unknown 17.5 K variant. S1 mapping analysis using human pituitary RNA confirms the existence of at least four distinct hGH mRNAs originating from alternative acceptor sites at the second intron of the primary transcript. We have analysed the hGH gene sequence to explain the high frequency of alternative splicings which occur only at this location. In this study we propose CTTGNNPyPyPy as an additional consensus sequence guiding the selection of the branched nucleotide. [less ▲]

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See detailNew Non-Overlapping Domain Decomposition Algorithm for the Helmholtz Equation
Boubendir, Y.; Antoine, X.; Geuzaine, Christophe ULg

in Proceedings of the 20th International Conference on Domain Decomposition Methods (DD20) (2011, February)

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See detailNew noncovalent inhibitors of penicillin-binding proteins from penicillin-resistant bacteria.
Turk, Samo; Verlaine, Olivier ULg; Gerards, Thomas ULg et al

in PloS one (2011), 6(5), 19418

BACKGROUND: Penicillin-binding proteins (PBPs) are well known and validated targets for antibacterial therapy. The most important clinically used inhibitors of PBPs beta-lactams inhibit transpeptidase ... [more ▼]

BACKGROUND: Penicillin-binding proteins (PBPs) are well known and validated targets for antibacterial therapy. The most important clinically used inhibitors of PBPs beta-lactams inhibit transpeptidase activity of PBPs by forming a covalent penicilloyl-enzyme complex that blocks the normal transpeptidation reaction; this finally results in bacterial death. In some resistant bacteria the resistance is acquired by active-site distortion of PBPs, which lowers their acylation efficiency for beta-lactams. To address this problem we focused our attention to discovery of novel noncovalent inhibitors of PBPs. METHODOLOGY/PRINCIPAL FINDINGS: Our in-house bank of compounds was screened for inhibition of three PBPs from resistant bacteria: PBP2a from Methicillin-resistant Staphylococcus aureus (MRSA), PBP2x from Streptococcus pneumoniae strain 5204, and PBP5fm from Enterococcus faecium strain D63r. Initial hit inhibitor obtained by screening was then used as a starting point for computational similarity searching for structurally related compounds and several new noncovalent inhibitors were discovered. Two compounds had promising inhibitory activities of both PBP2a and PBP2x 5204, and good in-vitro antibacterial activities against a panel of Gram-positive bacterial strains. CONCLUSIONS: We found new noncovalent inhibitors of PBPs which represent important starting points for development of more potent inhibitors of PBPs that can target penicillin-resistant bacteria. [less ▲]

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See detailNew norovirus classified as a recombinant GII.g/GII.1 causes an extended foodborne outbreak at a university hospital in Munich
Hoffman, Dieter; Mauroy, Axel ULg; Seebach, Judith et al

in Journal of Clinical Virology (2013), (58), 24-30

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See detailNew observational results on the colliding wind WR+O binary WR 30a
Gosset, Eric ULg; Royer, P.; Rauw, Grégor ULg et al

in Wolf-Rayet Phenomena in Massive Stars and Starburst Galaxies (1999)

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See detailNew observations and gravitational lens models of the Cloverleaf quasar H1413 + 117
Kayser, R.; Surdej, Jean ULg; Condon, J. J. et al

in Astrophysical Journal (1990), 364

New optical and radio observations of the quadruple quasar H1413 + 117, the Cloverleaf, are presented. VLA data show radio counterparts at the positions of the four optical images and an additional strong ... [more ▼]

New optical and radio observations of the quadruple quasar H1413 + 117, the Cloverleaf, are presented. VLA data show radio counterparts at the positions of the four optical images and an additional strong radio source between images B and D. Gravitational lens models of H1413 + 117 using (1) a single elliptical galaxy and (2) two spherical galaxies are presented, which fit the positions of the four images of the quasar remarkably well. The models suggest that the strong radio source is a feature of the quasar, e.g., an ejected blob, which lies right on the caustic of the lens and is thereby strongly amplified. The time delays predicted by these models are sufficiently short to allow for their determination within one observational season, making H1413 + 117 an exceptionally well suited object for gravitational lens research. [less ▲]

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See detailNew observations on some Upper Devonian brachiopods from the Namur-Dinant Basin (Belgium)
Mottequin, Bernard ULg

in Geodiversitas (2008), 30

Brachiopods (Productida [except Chonetidina], Orthida, Athyridida, Spiriferida [Adolfiidae, Reticulariidae, Ambocoeliidae, Thomasariidae], and Spiriferinida) recovered in the interval, in terms of the ... [more ▼]

Brachiopods (Productida [except Chonetidina], Orthida, Athyridida, Spiriferida [Adolfiidae, Reticulariidae, Ambocoeliidae, Thomasariidae], and Spiriferinida) recovered in the interval, in terms of the standard conodont zonation, between the Palmatolepis hassi and P. triangularis zones (Late Middle Frasnian to Early Famennian) in the Namur-Dinant Basin (Belgium) are described. The orders and suborders investigated include 32 species: 21 are described in open nomenclature, seven are previously known, four are new of which three are assigned to previously known genera: Aulacella aggeris n. sp., Dicamara plutonis n. sp., and Warrenella (Warrenella) aquaealbae n. sp. Th e fourth species belonging to a newly defined genus: Neptunathyris buxi n. gen., n. sp. A major brachiopod faunal change occurs at the top of the P. rhenana Zone in southern Belgium, with the disappearance of most existing species in parallel with the deterioration of the oxygenation conditions preceding the Upper Kelwasser Event. Only an impoverished fauna (Lingulida, Chonetidina, Rhynchonellida) has been collected in the P. linguiformis Zone. The recovery of the Famennian brachiopods after the end-Frasnian biotic crisis was rapid in the basal Famennian but, despite their great abundance, their diversity was relatively low. New cosmopolitan genera appeared at this time especially among the rhynchonellids, athyridids and spiriferids with new species of pre-existing orthid and orthotetid genera. [less ▲]

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See detailA new observed pre-copulatory behaviour of the lesser-spotted dogfish, scyliorhinus canicula, in captivity
Domi, Nadège; Poncin, Pascal ULg; Voss, Jacques

in Société française d'Ichtyologie (1999)

Detailed reference viewed: 73 (3 ULg)