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See detailMicrobiological state of the art of walloon farm raw milk butter (2006 – 2009).
Godrie, Thérèse ULg; Di Tanna, Sybille ULg; Helleputte, Murielle ULg et al

in Fourteenth conference on food microbiology (2009, June)

For several years, Walloon Region has been supporting financially a project to help farmers: la Cellule Qualité Produits Fermiers (CQPF). This project aims to accompany farmers in their diversification ... [more ▼]

For several years, Walloon Region has been supporting financially a project to help farmers: la Cellule Qualité Produits Fermiers (CQPF). This project aims to accompany farmers in their diversification activities. In this context, Agricultural University of Gembloux, Department of Food Technology and “Centre d’Expertise pour la Qualité” have ensured and are ensuring autocontrol system implementation based on the HACCP principles, together with the application of good hygiene practice. In this context, microbiological analyses on farm raw milk butter have been performed. Results collected during the period from September 2006 to April 2009 are presented below. [less ▲]

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See detailMicrobiological zoonotic emerging risks, transmitted between livestock animals and humans (2007-2015)
Filippitzi, ME; Goumperis, T; Robinson, T et al

in Transboundary and emerging diseases (in press)

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See detailLa microbiologie prévisionnelle : un nouvel outil de maîtrise de la qualité microbiologique des denrées alimentaires
Delhalle, Laurent ULg; Clinquart, Antoine ULg; Daube, Georges ULg

in Food Science and Law (2010), 2

Les nouveaux outils de microbiologie prévisionnelle permettent de mieux comprendre et de maîtriser le comportement des micro-organismes dans la chaîne alimentaire. La législation européenne récente, dont ... [more ▼]

Les nouveaux outils de microbiologie prévisionnelle permettent de mieux comprendre et de maîtriser le comportement des micro-organismes dans la chaîne alimentaire. La législation européenne récente, dont le but est garantir un niveau élevé de sécurité sanitaire des aliments proposés au consommateur, mentionne cette méthodologie comme outil de référence. Les autorités en charge de la gestion de la santé publique et les industriels ont maintenant à leur disposition des outils leur permettant de comprendre les flux de contamination dans la chaîne alimentaire, de fixer une date limite de consommation, d’optimaliser les processus de transformation et de conservation, et de proposer des aliments sûrs aux consommateurs. Cette discipline offre la possibilité, à travers ses modèles et ses concepts, d’exploiter des données issues d’expérimentations réalisées au laboratoire ou en production et de les convertir en applications qui permettent d’évaluer la stabilité et la sécurité d’une denrée alimentaire. [less ▲]

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See detailMicrobiologische risico's in verbans met voeding
Daube, Georges ULg

in Institut Danone (Ed.) Boesdselveliligheid : een wetenschapelijke benadering (2003)

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See detailThe microbiome from the Lyme disease principal reservoir host in southern Quebec (Peromyscus leucopus)
André, Adrien ULg; Mouton, Alice ULg; Millien, Virginie et al

Poster (2015, June 25)

The emergence of the Lyme disease in Southern Quebec appears directly linked to the recent arrival in the region of the rodent Peromyscus leucopus. Indeed, this species is considered to be the principal ... [more ▼]

The emergence of the Lyme disease in Southern Quebec appears directly linked to the recent arrival in the region of the rodent Peromyscus leucopus. Indeed, this species is considered to be the principal reservoir of the bacteria Borrelia Burgdorferei, responsible of the Lyme disease and the recent climatic warming has allowed the mice to colonize higher latitude territories. Other factors, like the regulation made by pathogens might as well play an important role in the dynamic of expansion of P. leucopus. In our project, we sampled several populations of P. leucopus from the North American border, where the species is thought to be present for 30 years, to the most recently colonized zones, situated approximatively 150km inside of the Quebec province. A characterisation of their microbiome was then performed from their liver, spleen and lungs. Our objectives are threefold: First, we settled a protocol based on NGS methods for the detection of Borrelia Burgdorferei in micro-mammal internal organs. Second, we plan to identify the Borreliosis infection zones and to study their spatio-temporal evolution. Third, we aim to test which of the central-marginal hypothesis or the enemy release hypothesis is best describing the scenario presently happening in southern Quebec concerning the distribution’s expansion of P. leucopus. These information will be of great interest to understand the dynamic of emergence of the Borreliosis and to predict the current and future distribution of this disease in order to inform the Canadian health authorities. [less ▲]

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See detailMICROBIOMED: Planar optic chip manufacturing and read-out
Fleury-Frenette, Karl ULg; Hastanin, Juriy ULg; Lenaerts, Cedric ULg et al

Poster (2014, September 11)

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See detailLa microbiopsie musculaire : un nouvel outil pour le suivi sportif et la détection précoce des dysfonctions musculaires
Votion, Dominique ULg; Fraipont, Audrey ULg; Robert, Céline et al

in 36ème Journée de la Recherche Equine (2010)

The objective of this study was to confirm the practical value of high-resolution respirometry (HRR) applied to biopsies to determine, in horses, the level of training, their athletic ability and for the ... [more ▼]

The objective of this study was to confirm the practical value of high-resolution respirometry (HRR) applied to biopsies to determine, in horses, the level of training, their athletic ability and for the early detection of muscular dysfunction. Materials and methods – The muscle mitochondrial respiration was determined by HRR in 20 endurance horses and 10 trotters sampled at the triceps brachii at different stages of their training. Results – Training increases mitochondrial respiration, in addition, the best performers had the highest rate of respiration. A trotter had abnormally low levels of muscle mitochondrial respiration for its level of training. This horse has presented several episodes of rhabdomyolysis during its racing season. Discussion – The biopsy is easily achievable by the attending veterinarian. This study shows that the athletic ability of horses is closely linked to respiratory muscle function, and suggests the value of HRR for performance prediction. In addition, the RHR has the ability to demonstrate mitochondrial dysfunction potentially responsible for exercise-induced rhabdomyolysis. [less ▲]

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See detailMicrobiopsies mammaires sous échographie
COLIN, Claude; LIFRANGE, Eric ULg

in Le Sein (1994), 4

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See detailMicrobiota characterization of a protected designation of origin Belgian cheese: Herve cheese, using metagenomic analysis.
Delcenserie, Véronique ULg; Taminiau, Bernard ULg; Delhalle, Laurent ULg et al

in Journal of Dairy Science (2014), 97

Herve cheese is a Belgian soft cheese with a washed rind, and is made from raw or pasteurized milk. The specific microbiota of this cheese has never previously been fully explored and the use of raw or ... [more ▼]

Herve cheese is a Belgian soft cheese with a washed rind, and is made from raw or pasteurized milk. The specific microbiota of this cheese has never previously been fully explored and the use of raw or pasteurized milk in addition to starters is assumed to affect the microbiota of the rind and the heart. The aim of the study was to analyze the bacterial microbiota of Herve cheese using classical microbiology and a metagenomic approach based on 16S ribosomal DNA pyrosequencing. Using classical microbiology, the total counts of bacteria were comparable for the 11 samples of tested raw and pasteurized milk cheeses, reaching almost 8 log cfu/g. Using the metagenomic approach, 207 different phylotypes were identified. The rind of both the raw and pasteurized milk cheeses was found to be highly diversified. However, 96.3 and 97.9% of the total microbiota of the raw milk and pasteurized cheese rind, respectively, were composed of species present in both types of cheese, such as Corynebacterium casei, Psychrobacter spp., Lactococcus lactis ssp. cremoris, Staphylococcus equorum, Vagococcus salmoninarum, and other species present at levels below 5%. Brevibacterium linens were present at low levels (0.5 and 1.6%, respectively) on the rind of both the raw and the pasteurized milk cheeses, even though this bacterium had been inoculated during the manufacturing process. Interestingly, Psychroflexus casei, also described as giving a red smear to Raclettetype cheese, was identified in small proportions in the composition of the rind of both the raw and pasteurized milk cheeses (0.17 and 0.5%, respectively). In the heart of the cheeses, the common species of bacteria reached more than 99%. The main species identified were Lactococcus lactis ssp. cremoris, Psychrobacter spp., and Staphylococcus equorum ssp. equorum. Interestingly, 93 phylotypes were present only in the raw milk cheeses and 29 only in the pasteurized milk cheeses, showing the high diversity of the microbiota. Corynebacterium casei and Enterococcus faecalis were more prevalent in the raw milk cheeses, whereas Psychrobacter celer was present in the pasteurized milk cheeses. However, this specific microbiota represented a low proportion of the cheese microbiota. This study demonstrated that Herve cheese microbiota is rich and that pasteurized milk cheeses are microbiologically very close to raw milk cheeses, probably due to the similar manufacturing process. The characterization of the microbiota of this particular protected designation of origin cheese was useful in enabling us to gain a better knowledge of the bacteria responsible for the character of this cheese. [less ▲]

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See detailMicrocalorimetry as applied to psychrophilic enzymes
D'Amico, Salvino ULg

Conference (2002)

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See detailMicrocalorimetry as applied to psychrophilic enzymes
D'Amico, Salvino ULg; Georlette, D.; Collins, T. et al

in Ladbury, J. E. (Ed.) Biocalorimetry 2: Application of Calorimetry in the Biological Sciences (2003)

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See detailMicrocapsules/microparticles loaded with biologically active molecules for medicine
Markvicheva, E; Selina, O; Stashevskaya, K et al

in Proceedin of European Congress on Chemical Engineering (2005, September 25)

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See detailMicrocarriers for animal cell culture
Markvicheva, Elena; Grandfils, Christian ULg

in Fundamentals of Cell Immobilisation Biotechnology, Series Focus on Biotechnology (2004)

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See detailMICROCARRIERS LOADED WITH BIOACTIVE MOLECULES FOR TISSUE ENGINEERING
Markvicheva, Elena; Drozdova, Maria; Zlobina, M. et al

in Bioencapsulation Innovations, Newsletter (2015), November

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See detailMICROCARRIERS LOADED WITH BIOACTIVE MOLECULES FOR TISSUE ENGINEERING
Markvicheva, Elena; Drozdova, Maria; Zlobina, M. et al

in Poncelet, Denis (Ed.) Proceeding of 23th International Conference on Bioencapsulation (2015)

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See detailMicrocephaly, muscular build, rhizomelia, and cataracts: Description of a possible recessive syndrome and some comments on the use of electronic databases in syndromology
Verloes, Alain ULg; Lesenfants, S.; Misson, Jean-Paul ULg et al

in American Journal of Medical Genetics (1997), 68(4), 455-60461

We report on a 7-year-old boy born of consanguineous parents with severe microcephaly (-5 SD) but borderline intelligence, juvenile cataract, muscular build, rhizomelic shortness of limbs predominantly of ... [more ▼]

We report on a 7-year-old boy born of consanguineous parents with severe microcephaly (-5 SD) but borderline intelligence, juvenile cataract, muscular build, rhizomelic shortness of limbs predominantly of femora, advanced bone age, and micropenis. This combination of signs appears unique and may represent an undescribed, possibly autosomal recessive MCA syndrome. The use of LDDB and POSSUM in the workup of such "new syndromes" is reviewed. Three search strategies are discussed: single rare sign browsing, best combinatory fit using an array of key words, and combined rare signs scan. Pitfalls in the use of such databases and the some problems raised by inconsistent/ incomplete encoding in those two popular, highly useful syndromology retrieval systems are discussed. [less ▲]

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See detailMicrochirurgie cordale et implantation d'acide hyaluronique esterifié
FINCK, Camille ULg; Harmegnies, Bernard; REMACLE, Angélique ULg

Conference (2007, October 16)

La microchirurgie cordale a deux buts : améliorer les caractéristiques vocales subjectives et objectives du patient porteur d’une lésion bénigne , ainsi qu’augmenter le rendement aérodynamique laryngé. La ... [more ▼]

La microchirurgie cordale a deux buts : améliorer les caractéristiques vocales subjectives et objectives du patient porteur d’une lésion bénigne , ainsi qu’augmenter le rendement aérodynamique laryngé. La qualité du résultat fonctionnel obtenu dépend de l’amélioration des capacités vibrantes de la corde opérée , et en particulier, de la souplesse de sa couche épithéliale et de son espace de Reinke. Moduler la cicatrisation cordale par l’implantation, en fin d’intervention, d’acide hyaluronique (AHE) estérifié dans l’espace de Reinke disséqué , a fait l’objet d’une précédente étude pilote chez 11 patients (1). Les résultats obtenus sont encourageants: bonne tolérance de l’implant et amélioration post-chirurgicale de la souplesse cordale sont observés dans tous les cas. L’étude actuelle porte sur 83 patients opérés entre février 2003 et février 2007. Les patients sont répartis en 2 groupes : 50 patients (groupe contrôle) bénéficiant d’une microchirurgie cordale sans implantation d’AHE (12 oedèmes, 4 kystes muqueux, 14 polypes, 7 nodules, 13 pseudokystes) et 33 patients (groupe AHE) bénéficiant d’une microchirurgie cordale suivie de l’implantation d’AHE dans l’espace de Reinke disséqué(6 oedèmes, 5 kystes muqueux, 4 polypes, 6 nodules,1 sulcus, 2 cicatrices, 2 fibroses sous-épithéliales, 3 vergetures, 4 kystes épidermoïdes ouverts). L’évaluation laryngée et vocale est pratiquée lors d’une consultation préopératoire, d’une consultation postopératoire immédiate et de plusieurs (1 à 6) consultations postopératoires différées. L’excellente tolérance de l’implant est par ailleurs confirmée par cette plus grande série. Deux études statistiques sont pratiquées afin d’évaluer d’une part le résultat postopératoire immédiat et d’autre part, l’évolution fonctionnelle à long terme. Conclusions : L’implantation d’AHE est bien toléré. Les résultats fonctionnels immédiats chez les patients bénéficiant de l’implantation d’AHE sont excellents et superposables au groupe témoin. Les patients implantés démontrent un continuum évolutif, à long terme, de différentes caractéristiques vocales et laryngées, bien au-delà de la phase postopératoire immédiate. A l’inverse, les patients du groupe contrôle, non implanté, n’évoluent plus au-delà de la phase post-opératoire immédiate. [less ▲]

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