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See detailOXA carbapenemase and their inhibition
Kerff, Frédéric ULg

Conference (2010, April 13)

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See detailOXA-198, an acquired carbapenem-hydrolyzing class D beta-lactamase from Pseudomonas aeruginosa.
El Garch, Farid; Bogaerts, Pierre; Bebrone, Carine ULg et al

in Antimicrobial Agents and Chemotherapy (2011), 55(10)

A carbapenem-resistant Pseudomonas aeruginosa strain (PA41437) susceptible to expanded-spectrum cephalosporins was recovered from several consecutive lower-respiratory-tract specimens of a patient who ... [more ▼]

A carbapenem-resistant Pseudomonas aeruginosa strain (PA41437) susceptible to expanded-spectrum cephalosporins was recovered from several consecutive lower-respiratory-tract specimens of a patient who developed a ventilator-associated pneumonia while hospitalized in an intensive care unit. Cloning experiments identified OXA-198, a new class D β-lactamase which was weakly related (less than 45% amino acid identity) to other class D β-lactamases. Expression in Escherichia coli TOP10 and in P. aeruginosa PAO1 led to transformants that were resistant to ticarcillin and showed reduced susceptibility to carbapenems and cefepime. The bla(OXA-198) gene was harbored by a class 1 integron carried by a ca. 46-kb nontypeable plasmid. This study describes a novel class D β-lactamase involved in carbapenem resistance in P. aeruginosa. [less ▲]

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See detailOxford Centre for Evidence‐Based Medicine 2011 Levels of Evidence ‐ Traduction française
OCEBM Levels of Evidence Working Group; Durieux, Nancy ULg; Pasleau, Françoise ULg et al

E-print/Working paper (2012)

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See detailOxidant activity of rabbit synoviocytes (HIG-82) demonstrated by oxymetry and ethylene production.
Schneider, Nicole ULg; Lejeune, Jean-Philippe ULg; Mouithys-Mickalad, Ange ULg et al

Poster (2003)

We are interested in a possible role of synoviocytes in the ROS production implicated in osteoarthritis, therefore we studied the response of a rabbit synoviocyte cell line (HIG-82) to variable oxygen ... [more ▼]

We are interested in a possible role of synoviocytes in the ROS production implicated in osteoarthritis, therefore we studied the response of a rabbit synoviocyte cell line (HIG-82) to variable oxygen tensions and the oxidant activity of these cells in response to stimuli. Synoviocytes were cultured at 5 and 21 % O2, their O2 consumption (cellular respiration, monitored with Clark electrode) was measured at 21% O2 and after anoxia, before and after stimulation with phorbol myristate acetate (PMA), and their oxidant response to PMA stimulation was quantified by measuring ethylene (gas chromatography) released when the substrate, alpha-keto-gamma-methylbutyric acid, is oxidised by the ROS produced by the cells. Cell growth was faster at 21 % O2 than at 5% O2, and microscopic observation revealed 2 cell populations: a few small round cells in suspension and many adherent cells. By oxymetry, we observed that a 106 synoviocytes suspension in 2 ml completely consumed O2 within 15 min, that anoxia (7 min) slightly slowed the respiration rate down and that PMA stimulation increased O2 consumption (150 % increase). The oxidant activity (ethylene production) of the cells was stimulated by PMA in a dose-dependent manner (10-9 to 10-7M) but the cell response was highly variable (from 150 to 1500 % increase) and was largely reduced by diphenyliodonium, an inhibitor of NADPH-oxidase and NO-synthase. The capacity to produce free radical species was confirmed for the small round cells by detection of an electron paramagnetic resonance (EPR) signal after stimulation. These results thus demonstrate a sensibility to O2 and an oxidant activity of synoviocytes at least related to ROS production by NADPH-oxidase activity. [less ▲]

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See detailOxidant-Scavenging Activities of Beta-Lactam Agents
Carreer, R.; Deby-Dupont, G.; Deby, C. et al

in European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology (1998), 17(1), 43-6

The relative antioxidant effect of ampicillin, ceftazidime, ceftriaxone, and cefuroxime on oxygen-reactive species was examined in vitro using stimulated human polymorphonuclear neutrophils. There was no ... [more ▼]

The relative antioxidant effect of ampicillin, ceftazidime, ceftriaxone, and cefuroxime on oxygen-reactive species was examined in vitro using stimulated human polymorphonuclear neutrophils. There was no evidence that any of the beta-lactam agents tested had an effect on superoxide or H2O2 generation. In contrast, all of the beta-lactam agents prevented hypochlorous acid (HOCI) chlorination of 1,1-dimethyl-4-chloro-3,5-cyclo-hexanedione in a cell-free system at concentrations of < 10 microg/ml. Furthermore, all antibiotics provided dose-dependent protection against HOCI cytotoxicity to 16HBE140 bronchial epithelial cells. Taken together, these data indicate a possible therapeutic role for beta-lactam agents in protecting host tissues from HOCI-induced oxidative damage. [less ▲]

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See detailThe oxidant/antioxidant equilibrium in horses
Kirschvink, Nathalie; de Moffarts, Brieuc; Lekeux, Pierre ULg

in Veterinary Journal (2008), 177(2), 178-191

Since "free radical research" started in 1954, understanding the role of oxidants and antioxidants in physiological and pathological conditions has increased continuously. Oxidants are essentially ... [more ▼]

Since "free radical research" started in 1954, understanding the role of oxidants and antioxidants in physiological and pathological conditions has increased continuously. Oxidants are essentially generated by metabolic enzymes, inflammatory cells and mitochondrial electron leakage; they are indispensable for the cellular redox regulation and may, under certain conditions, have a pro-inflammatory stimulatory role. Endogenous and exogenous antioxidants counterbalance the oxidative processes and so maintain the oxidant/antioxidant equilibrium. Excessive oxidant generation or antioxidant insufficiency can lead to oxidative stress. The aims of this review are: (1) to provide an insight into the concept of the oxidant/antioxidant equilibrium by briefly introducing the oxidant and the antioxidant systems; (2) to describe how the oxidant/antioxidant equilibrium or oxidative stress can be evaluated in horses, and (3) to summarise current knowledge about oxidative stress in equine medicine and equine exercise physiology. [less ▲]

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See detailOxidants and airway inflammation
Kirschvink, N.; Lekeux, Pierre ULg

in 3rd World Equine Airways Symposium (WEAS) (2005)

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See detailOxidation and orally administrated 'naturally labeled 13C-glucose' during prolonged muscular exercise : 100g versus 4 x 250g
Pirnay, Freddy ULg; Krzentowski, G.; Crielaard, Jean-Michel ULg et al

in Poortmans, J. (Ed.) Biochemistry of exercise IV-A : 4th International Symposium of Biochemistry on Exercise, June 19-22, 1979, Bruxelles (1981)

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See detailOxidation des allylséléniures par les réactifs AD de Sharpless
Colaux, Catherine ULg; Krief, Alain; Dumont, willy

Poster (1998, September)

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See detailOxidation Mechanism in SiCO: First-Principles Study of O2 Diffusion and Reaction
Xu, Bin ULg; Kroll, Peter

Conference (2010, August)

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See detailOxidation of allylselenides with the Sharpless AD reagents
Colaux, Catherine ULg; Krief, alain; Dumont, Willy

Poster (1998, July)

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See detailOxidation of allylselenides with the Sharpless AD reagents
Colaux, Catherine ULg; Krief, Alain; Dumont, Willy

Poster (1997, December)

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See detailOxidation of allylselenides with the Sharpless AD-reagents
Krief, Alain; Colaux, Catherine ULg; Dumont, willy

in Tetrahedron Letters (1997), 38(18), 3315-3318

Allylselenides are efficiently oxidized by AD-mix reagents. Oxidation takes chemoselectively place on the selenium atom of allylselenides bearing a methylseleno or a phenylseleno moiety, but ... [more ▼]

Allylselenides are efficiently oxidized by AD-mix reagents. Oxidation takes chemoselectively place on the selenium atom of allylselenides bearing a methylseleno or a phenylseleno moiety, but chemoselectively occurs on the C,C double bond of the corresponding o-nitrophenyl derivatives. [less ▲]

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See detailOxidation of organic substrates with potassium ferrate (VI) in the presence of the K10 montmorillonite
Delaude, Lionel ULg; Laszlo, Pierre ULg; Lehance, Pascal

in Tetrahedron Letters (1995), 36(46), 8505-8508

Among various aluminosilicate supports, the K10 montmorillonite clay was the best catalyst for the oxidation of organic substrates with potassium ferrate (VI). Thus, a solid mixture of K2FeO4 and K10 has ... [more ▼]

Among various aluminosilicate supports, the K10 montmorillonite clay was the best catalyst for the oxidation of organic substrates with potassium ferrate (VI). Thus, a solid mixture of K2FeO4 and K10 has been used for the efficient oxidation of selected alcohols, benzylamine, thiophenol, and aniline in hydrocarbon solvents at room temperature. Somewhat humid clay performs best, and it is important to control the amount of water associated to the solid support. [less ▲]

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See detailOxidation Sensitivity May Be a Useful Tool for the Detection of the Hematotoxic Potential of Newly Developed Molecules: Application to Antipsychotic Drugs
Liégeois, Jean-François ULg; Bruhwyler, J.; Petit, C. et al

in Archives of Biochemistry & Biophysics (1999), 370(1), 126-37

Some antipsychotic agents have been found to produce agranulocytosis and aplastic anemia. The oxidation phenomena and/or the formation of free radicals has been suggested to be causally related to various ... [more ▼]

Some antipsychotic agents have been found to produce agranulocytosis and aplastic anemia. The oxidation phenomena and/or the formation of free radicals has been suggested to be causally related to various hematological disorders, e.g., agranulocytosis. Using five experimental conditions, we tested the oxidative potential of compounds with and without a history of hematological side effects, e.g., agranulocytosis and aplastic anemia. A statistical analysis was undertaken for each experimental condition and a multivariate analysis combining all results was performed. Two peroxidase-induced free radical models did not successfully discriminate between drugs with and without a history of causing hematologic problems (<70%). The lipid peroxidation system provided even less satisfactory discrimination, with only 56.25% correct classification. However, an 87.5% correct classification was obtained when using the oxidation potentials of these drugs determined at pH 4.7 and at pH 7.4. A multivariate analysis taking into account the five variables provided 87.5% success in classification. The two clusters were better discriminated in terms of a "distance coefficient." In a second analysis, the putative antipsychotic pyridobenzodiazepine analogues (JL5, JL8, JL18, and JL25) were classified in the cluster of toxic compounds, while the oxa- and thiazepine analogues (JL2, JL3, and JL13) were classified as nontoxic compounds. On the other hand, a few metabolites of clozapine and fluperlapine were classified in the toxic compound group. The procedure described herein is, to our knowledge, the first which classifies molecules of different structures as well as different pharmacological profiles according to their hematotoxic potential. Such a procedure could be used to predict drug-induced hematological side effects. [less ▲]

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See detailOxidative degradations of oxodegradable LDPE enhanced with thermoplastic pea starch: thermo-mechanical properties, morphology, and UV-ageing studies
Raquez, Jean-Marie; Bourgeois, Aurore; Jacobs, Heidi et al

in Journal of Applied Polymer Science (2011), 122(1), 489496

The abiotic UV-degradation behavior of oxodegradable LDPE was investigated in the presence of thermoplastic pea starch (TPPS) in this study. Oxodegradable LDPE was first melt-blended with thermoplastic ... [more ▼]

The abiotic UV-degradation behavior of oxodegradable LDPE was investigated in the presence of thermoplastic pea starch (TPPS) in this study. Oxodegradable LDPE was first melt-blended with thermoplastic pea starch (TPPS) using an internal mixing chamber to enhance the abiotic oxidative degradation of oxodegradable LDPE. Because of their different affinity, maleated polyethylene was added as compatibilizer. Tensile properties, thermal properties, and morphology of resulting melt-blends were determined at different content in TPPS. High content in TPPS (40 wt %) could be readily added to oxodegradable LDPE without affecting the tensile properties of resulting melt-blends. UV-ageing studies on compatibilized TPPS/oxodegradable LDPE melt-blends were carried out by Attenuated Total Reflectance infrared spectroscopy (ATR-FTIR), Dynamic Thermomechanical Analyses (DMTA) and Differential Scanning Calorimetry (DSC) under abiotic conditions. These results suggested a synergistic effect on the UV-ageing of TPPS-based melt-blends provided by both components during the first stage of UV-irradiation. [less ▲]

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See detailOxidative induction time: a quality assurance measurement to predict environmental stress-cracking
Courard, Luc ULg; Rigo, Jean-Marie ULg

in De Groot, M.B.; Den Hoedt, G.; Termaat, R.J. (Eds.) Proceedings of the 1st European Geosynthetics Conference EUROGEO 1 (1996)

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See detailOxidative phosphorylation: Building blocks and related components
Cardol, Pierre ULg; Figueroa, Francisco; Remacle, Claire ULg et al

in Stern, David; Harris, Elizabeth; Witman, George (Eds.) The Chlamydomonas Sourcebook 3-vol set, 1-3 (2009)

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See detailOxidative Processes in Human Promonocytic Cells (Thp-1) after Differentiation into Macrophages by Incubation with Chlamydia Pneumoniae Extracts
Mouithys-Mickalad, Ange ULg; Deby-Dupont, Ginette; Nys, Monique ULg et al

in Biochemical and Biophysical Research Communications (2001), 287(3), 781-8

Human monocytes differentiated into macrophages by Chlamydia pneumoniae were able to oxidize blood lipoproteins, as discovered by Kalayoglu et al. (1998). Using a model of human promonocytic cells (THP-1 ... [more ▼]

Human monocytes differentiated into macrophages by Chlamydia pneumoniae were able to oxidize blood lipoproteins, as discovered by Kalayoglu et al. (1998). Using a model of human promonocytic cells (THP-1), the cells were differentiated into macrophages by preincubation with C. pneumoniae extract, and further stimulated by phorbol myristate acetate. In these conditions, the differentiated cells oxidized a thiol compound and released superoxide anion as demonstrated respectively by gas liquid chromatography and electron spin resonance. The thiol oxidation and superoxide anion release were inhibited by diphenyliodonium, a NADPH oxidase and NOsynthase inhibitor, proving that the respiratory burst and the NOsynthase were involved in the oxidation processes occurring in the differentiated THP-1. The role of H(2)O(2) (derived from superoxide anion) was indicated by the enhancing effect of a peroxidase on the thiol oxidation. The presence of alpha-tocopherol in the surrounding medium strongly diminished the oxidation of the thiol target. [less ▲]

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See detailOxidative Stress in Clinical Situations--Fact or Fiction?
Pincemail, Joël ULg; Defraigne, Jean-Olivier ULg; Limet, Raymond ULg

in European Journal of Anaesthesiology (1996), 13(3), 219-34

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