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See detailOverproduction and properties of the mannuronate alginate lyase AlxMB
Malissard, Martine; Chavagnat, Frédéric; Duez, Colette ULg et al

in FEMS Microbiology Letters (1995), 126(2), 105-111

In previous studies (Malissard et al., FEMS Microbiol. Lett. (1993) 110, 101-106), the alginate lyase AlxM of the marine bacterium ATCC 433367 was produced in Escherichia coli TC4/pAL-A3 with a yield of ... [more ▼]

In previous studies (Malissard et al., FEMS Microbiol. Lett. (1993) 110, 101-106), the alginate lyase AlxM of the marine bacterium ATCC 433367 was produced in Escherichia coli TC4/pAL-A3 with a yield of 50 mu g per litre of culture. The polypeptide chain was cleaved between two cysteine residues, C169 and C183, themselves linked by a disulphide bridge. AlxM has now been overproduced in E. coli BL21(DE3)/pAL-Sur/pLysS. Under conditions in which formation of inclusion bodies can be avoided, the enzyme is synthesized as a catalytically active, water-soluble, unnicked polypeptide with a yield of 32 mg per litre of culture. It has been purified to protein homogeneity using a one-step procedure. The nicked ALxM(A) and unnicked ALxM(B) alginate lyases have identical alginate-degrading activities at high salt concentrations. [less ▲]

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See detailOverproduction of g-decalactone by short-chain acyl-CoA oxidase blockage in Yarrowia lipolytica
Waché, Y.; Aguedo, Mario ULg; LeDall, M.-T. et al

Conference (1999, September)

Detailed reference viewed: 14 (1 ULg)
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See detailOverproduction of lipase by Yarrowia lipolytica mutants
Fickers, Patrick ULg; Nicaud, J. M.; Destain, Jacqueline ULg et al

in Applied Microbiology & Biotechnology (2003), 63(2), 136-142

Non-genetically modified mutants with increased capacities of extracellular lipase production were obtained from Yarrowia lipolytica strain CBS6303 by chemical mutagenesis. Of the 400 mutants isolated ... [more ▼]

Non-genetically modified mutants with increased capacities of extracellular lipase production were obtained from Yarrowia lipolytica strain CBS6303 by chemical mutagenesis. Of the 400 mutants isolated, LgX64.81 had the highest potential for the development of an industrial lipase production process. This mutant exhibits lipase production uncoupled from catabolite repression by glucose, and a 10-fold increased productivity upon addition of oleic acid. Using a LIP2-LacZ reporter gene, we demonstrate that the mutant phenotype originates from a trans-acting mutation. The glucose uptake capacity of LgX64.81 is reduced 2.5-fold compared to the wild-type-strain, and it exhibits high lipase production on glucose medium. A trans-acting mutation in a gene involved in glucose transport could thus explain this mutant phenotype. [less ▲]

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See detailOverriding the norm: the use of gender-marked personal pronouns in spoken language
De Vos, Lien ULg

Scientific conference (2011, March 18)

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See detailOvershooting and semiconvection: structural changes and asteroseismic signatures
Noels-Grötsch, Arlette ULg; Montalban Iglesias, Josefa ULg; Miglio, Andrea ULg et al

in Astrophysics & Space Science (2010), 328

Overshooting and semiconvection are two poorly known mechanisms which affect the extent and the efficiency of chemical mixing outside classical convection zones in stars. We discuss the uncertainties and ... [more ▼]

Overshooting and semiconvection are two poorly known mechanisms which affect the extent and the efficiency of chemical mixing outside classical convection zones in stars. We discuss the uncertainties and the inferences of those processes in main sequence stars burning hydrogen in a convective core. We then focus on the asteroseismic signatures of partially or fully mixed zones surrounding the convective core, through the detailed shape of the induced chemical composition profile. We emphasize the potential power of asteroseismology to determine the internal structure of stars and thus to help us understand the physical processes at work inside the stars. [less ▲]

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See detailOvershootings and spurious oscillations caused by biharmonic mixing
Delhez, Eric ULg; Deleersnijder, Eric

in Ocean Modelling (2007), 17(3), 183-198

Biharmonic mixing is often used in large scale numerical models of the ocean because of its scale selectivity; it effectively damps small scale noise and leaves the large scale dynamics nearly unaffected ... [more ▼]

Biharmonic mixing is often used in large scale numerical models of the ocean because of its scale selectivity; it effectively damps small scale noise and leaves the large scale dynamics nearly unaffected. The biharmonic operator lacks however positiveness and monotonicity and can therefore produce unphysical results exhibiting spurious overshootings and oscillations. This problematic behaviour cannot be avoided by the addition of an ordinary Laplacian diffusion term. It appears in both continuous and discrete approaches/solutions in both unbounded and bounded domains. The overshootings and oscillations are induced by the strong damping of the smaller scale modes and are therefore comparable to the Gibbs' phenomenon. With appropriate boundary conditions, the variance of the field decreases monotonically and the oscillations are expected to remain small. The lack of positiveness is however a severe drawback for (dynamic) tracer studies. (C) 2007 Elsevier Ltd. All rights reserved. [less ▲]

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See detailOverview and Benchmarking of Motion Detection Methods
Jodoin, Pierre-Marc; Pierard, Sébastien ULg; Wang, Yi et al

in Bouwmans; Porikli; Hoferlin (Eds.) et al Background Modeling and Foreground Detection for Video Surveillance (2014)

In this chapter, we provide an overview of the most highly cited motion detection meth- ods. We identify the most commonly used background models together with their features, the kind of updating scheme ... [more ▼]

In this chapter, we provide an overview of the most highly cited motion detection meth- ods. We identify the most commonly used background models together with their features, the kind of updating scheme they use, some spatial aggregation models as well as the most widely used post-processing operations. We also provide an overview of datasets used to validate motion detection methods. Please note that this literature review is by no means exhaustive and thus we provide a list of surveys that the reader can rely on for further details. [less ▲]

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See detailOverview of animal related-accidents in one of the world’s densest road network region
Lehaire, François ULg; Morelle, Kevin ULg; Lejeune, Philippe ULg et al

Poster (2012, October)

With its 4.7 km/km² of public roads, Southern Belgium (Wallonia) has one of the densest road network of Western Europe. This network as well as the observed increase in main game species populations (red ... [more ▼]

With its 4.7 km/km² of public roads, Southern Belgium (Wallonia) has one of the densest road network of Western Europe. This network as well as the observed increase in main game species populations (red deer, roe deer and wild boar) make Wallonia an interesting region for studying the patterns of traffic accidents caused by animals. Moreover, compared to most of European countries, no statistics are available for this area. To shed light on the current situation of wildlife roadkills, the police database of traffic accident statement was thoroughly investigated. Those statements concern accidents that occurred between 2006 and 2010. Each record includes date, time, species involved and information about the location of the accident event. Based on this dataset, we have analysed (i) composition and percentage of involved species, (ii) the consequence (material damages / bodily injuries), (iii) the spatial (type of roads, proximity to landscape features, ‘hot spot’ map) and (iv) temporal (daily, weekly and seasonal) distribution of animal-related accidents. In conclusion future research perspectives are presented. [less ▲]

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See detailOverview of CO2 dynamics within sea ice
Delille, Bruno ULg; Geilfus, Nicolas-Xavier ULg; Vancoppenolle, M. et al

Conference (2011)

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See detailOverview of cobalt-mediated radical polymerization: roots, state of the art and future prospects
Debuigne, Antoine ULg; Poli, Rinaldo; Jérôme, Christine ULg et al

in Progress in Polymer Science (2009), 34(3), 211-239

Controlled radical polymerization (CRP) techniques offer the opportunity to properly design polymer chains and adjust their chemical and physical properties. Among these techniques, cobalt-mediated ... [more ▼]

Controlled radical polymerization (CRP) techniques offer the opportunity to properly design polymer chains and adjust their chemical and physical properties. Among these techniques, cobalt-mediated radical polymerization (CMRP) distinguished itself by the high level of control imparted to the polymerization of acrylic and vinyl ester monomers, even for high molar masses. This article summarizes for the first time the advances in understanding and synthetic scope of CMRP since its discovery. Notably, the cobalt–carbon bond formation by dual contribution of reversible termination and degenerative chain transfer is discussed, as well as the impact of additives able to coordinate the metal. The potential of computational chemistry in the field of CMRP as a rationalization and predicting tool is also presented. These mechanistic considerations and achievements in macromolecular engineering will be discussed along with challenges and future prospects in order to assess the CMRP system as a whole. [less ▲]

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See detailOverview of european national forestinventories : attempt to a comparison
Rondeux, Jacques ULg

in Testlauf zur Landeswaldinventur : Konzeption, Verfahrenstest, Ergebnisse- (1997)

Detailed reference viewed: 8 (0 ULg)
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See detailOverview of fire design
Franssen, Jean-Marc ULg; Wald, F.; Burgess, I. et al

in Mazzolani, Federico (Ed.) Proceedings of Urban Habitat Constructions under Catastrophic Events (COST Action C26) (2008)

Detailed reference viewed: 63 (7 ULg)
See detailOverview of fire design
Wang, Y. C.; Franssen, Jean-Marc ULg; Heinisuo et al

in Urban Habitat Constructions under catastrophic events - Technical sheets – Fire Resistance (2009)

Detailed reference viewed: 39 (1 ULg)
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See detailOverview of genetic testing in patients with pituitary adenomas
Beckers, Albert ULg; Rostomyan, Liliya ULg; Daly, Adrian ULg

in Annales d'Endocrinologie (2012), 73

Detailed reference viewed: 18 (5 ULg)
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See detailAn Overview of Interchanges and Ramps in Case of Sabzevar
Ismail, Amiruddin; Zourbakhsh, Mojtaba; Eftekhar, Hamed ULg et al

in Research Journal of Applied Sciences, Engineering and Technology (2014), 7(6), 977-980

Due to increase of the urbanization and raising the number of the vehicles road traffic incident and delay time in traffic jam are the main concern of countries in all over the world. Due to this problem ... [more ▼]

Due to increase of the urbanization and raising the number of the vehicles road traffic incident and delay time in traffic jam are the main concern of countries in all over the world. Due to this problem drivers face an elevated crash risk especially when drivers on freeway ramp interchanges compared with other sections of freeways. The definition of ramp was based on the type and number of lanes used by traffic to freeways. The vehicle accidents are prevalent on highway ramps because of over-speeding, related to the characteristics and circumstances of ramps. Site survey and observation has been done for each location to identify the road condition, adjacent environment and vehicle operations. SPSS is used to analysis the accidents that they are collected data from case study. Descriptive analyses are performed by output of the analyzing data of accident. Differences in accident rates are due to driver behavior, weather potential safety issues were identified at interchanges. Ramps are scheduled for auditing based on descending road speed limits. [less ▲]

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See detailOverview of Ionospheric-Magnetospheric Coupling at Jupiter: The Jovian Aurora
Waite, J. H.; Grodent, Denis ULg; Crary, F. et al

Conference (2001, June 25)

Detailed reference viewed: 4 (0 ULg)
See detailAn Overview Of Methodology For Predicting Activities And Functionalities Of A Wide Range Of Carbohydrate- Based Surfactants From Their Fundamental Properties
Razafindralambo, Hary ULg

Conference (2011, June)

Introduction and Background: Carbohydrate-based surfactants (CBS) are, today, among the most attractive amphiphilic compounds owing to their high potentiality for a large product concept. The main ... [more ▼]

Introduction and Background: Carbohydrate-based surfactants (CBS) are, today, among the most attractive amphiphilic compounds owing to their high potentiality for a large product concept. The main fundamental reasons are the abundance of their precursors from renewable resources, the excellent environmental compatibility, and the quasi-unlimited availability of their molecular structure and geometry. Based on the latter aspect, CBS would be expected to generate a wide range of functionalities and activities for food and non-food applications. All kinds of amphiphilic compounds including small surfactants, bolaforms, geminis, oligomeric, and polymeric molecules with a single or a multiple hydrophobic tails can be designed by synthetic routes thanks to a large number of reactive chemical groups in their basic structure. Consequently, various methodologies are required, and could be used in complementary for identifying all of their potentiality for further development. Methodology: The methodology is based on the characterization in the laboratory scale, i.e. using only small amounts of sample, of CBS properties at fluid and solid interfaces, in bulk liquid phases, and at the solid state with simplified models. Results and Discussion: In this communication, an overview of functionality and activity-predicting methods for a wide range of CBS structures varying in head polar groups, hydrophobic tail and number, and linkers will be presented and discussed. Performances and complementarities of different approaches, methodologies, and techniques will be emphasized. These include sample preparation and analysis procedures, interfacial-characterizing instruments, mainly tensiometers and Langmuir balances used both in dynamic and (quasi)-static modes, particle size and electrical charge analyser, and (micro)calorimeters using differential scanning, isothermal titration, and thermogravimetry methods. Conclusion: The knowledge of these basic properties is helpful for predicting their main activities and functionalities like their aptitude for forming and stabilizing colloidal systems, and for developing biological activities. This is also necessary for the rational design of CBS amphiphilic structure regarding to specific properties. The same approach can also be applied for other required features as the compound stability, toxicity, and biodegradability. [less ▲]

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See detailAn Overview Of Methodology For Predicting Activities And Functionalities Of A Wide Range Of Carbohydrate- Based Surfactants From Their Fundamental Properties
Razafindralambo, Hary ULg; Blecker, Christophe ULg; Paquot, Michel ULg

in 8th World surfactant congress and business convention (2011, June 06)

Introduction and Background: Carbohydrate-based surfactants (CBS) are, today, among the most attractive amphiphilic compounds owing to their high potentiality for a large product concept. The main ... [more ▼]

Introduction and Background: Carbohydrate-based surfactants (CBS) are, today, among the most attractive amphiphilic compounds owing to their high potentiality for a large product concept. The main fundamental reasons are the abundance of their precursors from renewable resources, the excellent environmental compatibility, and the quasi-unlimited availability of their molecular structure and geometry. Based on the latter aspect, CBS would be expected to generate a wide range of functionalities and activities for food and non-food applications. All kinds of amphiphilic compounds including small surfactants, bolaforms, geminis, oligomeric, and polymeric molecules with a single or a multiple hydrophobic tails can be designed by synthetic routes thanks to a large number of reactive chemical groups in their basic structure. Consequently, various methodologies are required, and could be used in complementary for identifying all of their potentiality for further development. Methodology: The methodology is based on the characterization in the laboratory scale, i.e. using only small amounts of sample, of CBS properties at fluid and solid interfaces, in bulk liquid phases, and at the solid state with simplified models. Results and Discussion: In this communication, an overview of functionality and activity-predicting methods for a wide range of CBS structures varying in head polar groups, hydrophobic tail and number, and linkers will be presented and discussed. Performances and complementarities of different approaches, methodologies, and techniques will be emphasized. These include sample preparation and analysis procedures, interfacial-characterizing instruments, mainly tensiometers and Langmuir balances used both in dynamic and (quasi)-static modes, particle size and electrical charge analyser, and (micro)calorimeters using differential scanning, isothermal titration, and thermogravimetry methods. Conclusion: The knowledge of these basic properties is helpful for predicting their main activities and functionalities like their aptitude for forming and stabilizing colloidal systems, and for developing biological activities. This is also necessary for the rational design of CBS amphiphilic structure regarding to specific properties. The same approach can also be applied for other required features as the compound stability, toxicity, and biodegradability. [less ▲]

Detailed reference viewed: 31 (14 ULg)
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See detailAn overview of NDSC-related activities at the Jungfraujoch through high-resolution infrared solar observations
Zander, Rodolphe ULg; Demoulin, Philippe ULg; Mahieu, Emmanuel ULg et al

in Bojkov, R.; Visconti, G. (Eds.) Proceedings of "The XXVIII Quadrennial Ozone Symposium" (1998)

Since the mid-1970s, infrared remote sensing at the Jungfraujoch, Switzerland, has been performed in response to the need to measure the concentrations and assess the seasonal and long-term changes of an ... [more ▼]

Since the mid-1970s, infrared remote sensing at the Jungfraujoch, Switzerland, has been performed in response to the need to measure the concentrations and assess the seasonal and long-term changes of an increasing number of atmospheric constituents. Currently, the vertical column abundances of over 20 telluric gases are being monitored, based on the analysis of solar spectra recorded regularly between 2 and 14 microns, using two high-resolution Fourier transform spectrometers. In this paper, emphasis is placed on those activities more specifically performed within the framework of the NDSC (Network for the Detection of Stratospheric Change) whose objectives are briefly described in the introduction. [less ▲]

Detailed reference viewed: 14 (2 ULg)