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See detailMicrobiota characterization of a protected designation of origin Belgian cheese: Herve cheese, using metagenomic analysis.
Delcenserie, Véronique ULg; Taminiau, Bernard ULg; Delhalle, Laurent ULg et al

in Journal of Dairy Science (2014), 97

Herve cheese is a Belgian soft cheese with a washed rind, and is made from raw or pasteurized milk. The specific microbiota of this cheese has never previously been fully explored and the use of raw or ... [more ▼]

Herve cheese is a Belgian soft cheese with a washed rind, and is made from raw or pasteurized milk. The specific microbiota of this cheese has never previously been fully explored and the use of raw or pasteurized milk in addition to starters is assumed to affect the microbiota of the rind and the heart. The aim of the study was to analyze the bacterial microbiota of Herve cheese using classical microbiology and a metagenomic approach based on 16S ribosomal DNA pyrosequencing. Using classical microbiology, the total counts of bacteria were comparable for the 11 samples of tested raw and pasteurized milk cheeses, reaching almost 8 log cfu/g. Using the metagenomic approach, 207 different phylotypes were identified. The rind of both the raw and pasteurized milk cheeses was found to be highly diversified. However, 96.3 and 97.9% of the total microbiota of the raw milk and pasteurized cheese rind, respectively, were composed of species present in both types of cheese, such as Corynebacterium casei, Psychrobacter spp., Lactococcus lactis ssp. cremoris, Staphylococcus equorum, Vagococcus salmoninarum, and other species present at levels below 5%. Brevibacterium linens were present at low levels (0.5 and 1.6%, respectively) on the rind of both the raw and the pasteurized milk cheeses, even though this bacterium had been inoculated during the manufacturing process. Interestingly, Psychroflexus casei, also described as giving a red smear to Raclettetype cheese, was identified in small proportions in the composition of the rind of both the raw and pasteurized milk cheeses (0.17 and 0.5%, respectively). In the heart of the cheeses, the common species of bacteria reached more than 99%. The main species identified were Lactococcus lactis ssp. cremoris, Psychrobacter spp., and Staphylococcus equorum ssp. equorum. Interestingly, 93 phylotypes were present only in the raw milk cheeses and 29 only in the pasteurized milk cheeses, showing the high diversity of the microbiota. Corynebacterium casei and Enterococcus faecalis were more prevalent in the raw milk cheeses, whereas Psychrobacter celer was present in the pasteurized milk cheeses. However, this specific microbiota represented a low proportion of the cheese microbiota. This study demonstrated that Herve cheese microbiota is rich and that pasteurized milk cheeses are microbiologically very close to raw milk cheeses, probably due to the similar manufacturing process. The characterization of the microbiota of this particular protected designation of origin cheese was useful in enabling us to gain a better knowledge of the bacteria responsible for the character of this cheese. [less ▲]

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See detailMicrocalorimetry as applied to psychrophilic enzymes
D'Amico, Salvino ULg

Conference (2002)

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See detailMicrocalorimetry as applied to psychrophilic enzymes
D'Amico, Salvino ULg; Georlette, D.; Collins, T. et al

in Ladbury, J. E. (Ed.) Biocalorimetry 2: Application of Calorimetry in the Biological Sciences (2003)

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See detailMicrocapsules/microparticles loaded with biologically active molecules for medicine
Markvicheva, E; Selina, O; Stashevskaya, K et al

in Proceedin of European Congress on Chemical Engineering (2005, September 25)

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See detailMicrocarriers for animal cell culture
Markvicheva, Elena; Grandfils, Christian ULg

in Fundamentals of Cell Immobilisation Biotechnology, Series Focus on Biotechnology (2004)

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See detailMICROCARRIERS LOADED WITH BIOACTIVE MOLECULES FOR TISSUE ENGINEERING
Markvicheva, Elena; Drozdova, Maria; Zlobina, M. et al

in Bioencapsulation Innovations, Newsletter (2015), November

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See detailMICROCARRIERS LOADED WITH BIOACTIVE MOLECULES FOR TISSUE ENGINEERING
Markvicheva, Elena; Drozdova, Maria; Zlobina, M. et al

in Poncelet, Denis (Ed.) Proceeding of 23th International Conference on Bioencapsulation (2015)

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See detailMicrocephaly, muscular build, rhizomelia, and cataracts: Description of a possible recessive syndrome and some comments on the use of electronic databases in syndromology
Verloes, Alain ULg; Lesenfants, S.; Misson, Jean-Paul ULg et al

in American Journal of Medical Genetics (1997), 68(4), 455-60461

We report on a 7-year-old boy born of consanguineous parents with severe microcephaly (-5 SD) but borderline intelligence, juvenile cataract, muscular build, rhizomelic shortness of limbs predominantly of ... [more ▼]

We report on a 7-year-old boy born of consanguineous parents with severe microcephaly (-5 SD) but borderline intelligence, juvenile cataract, muscular build, rhizomelic shortness of limbs predominantly of femora, advanced bone age, and micropenis. This combination of signs appears unique and may represent an undescribed, possibly autosomal recessive MCA syndrome. The use of LDDB and POSSUM in the workup of such "new syndromes" is reviewed. Three search strategies are discussed: single rare sign browsing, best combinatory fit using an array of key words, and combined rare signs scan. Pitfalls in the use of such databases and the some problems raised by inconsistent/ incomplete encoding in those two popular, highly useful syndromology retrieval systems are discussed. [less ▲]

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See detailMicrochirurgie cordale et implantation d'acide hyaluronique esterifié
FINCK, Camille ULg; Harmegnies, Bernard; REMACLE, Angélique ULg

Conference (2007, October 16)

La microchirurgie cordale a deux buts : améliorer les caractéristiques vocales subjectives et objectives du patient porteur d’une lésion bénigne , ainsi qu’augmenter le rendement aérodynamique laryngé. La ... [more ▼]

La microchirurgie cordale a deux buts : améliorer les caractéristiques vocales subjectives et objectives du patient porteur d’une lésion bénigne , ainsi qu’augmenter le rendement aérodynamique laryngé. La qualité du résultat fonctionnel obtenu dépend de l’amélioration des capacités vibrantes de la corde opérée , et en particulier, de la souplesse de sa couche épithéliale et de son espace de Reinke. Moduler la cicatrisation cordale par l’implantation, en fin d’intervention, d’acide hyaluronique (AHE) estérifié dans l’espace de Reinke disséqué , a fait l’objet d’une précédente étude pilote chez 11 patients (1). Les résultats obtenus sont encourageants: bonne tolérance de l’implant et amélioration post-chirurgicale de la souplesse cordale sont observés dans tous les cas. L’étude actuelle porte sur 83 patients opérés entre février 2003 et février 2007. Les patients sont répartis en 2 groupes : 50 patients (groupe contrôle) bénéficiant d’une microchirurgie cordale sans implantation d’AHE (12 oedèmes, 4 kystes muqueux, 14 polypes, 7 nodules, 13 pseudokystes) et 33 patients (groupe AHE) bénéficiant d’une microchirurgie cordale suivie de l’implantation d’AHE dans l’espace de Reinke disséqué(6 oedèmes, 5 kystes muqueux, 4 polypes, 6 nodules,1 sulcus, 2 cicatrices, 2 fibroses sous-épithéliales, 3 vergetures, 4 kystes épidermoïdes ouverts). L’évaluation laryngée et vocale est pratiquée lors d’une consultation préopératoire, d’une consultation postopératoire immédiate et de plusieurs (1 à 6) consultations postopératoires différées. L’excellente tolérance de l’implant est par ailleurs confirmée par cette plus grande série. Deux études statistiques sont pratiquées afin d’évaluer d’une part le résultat postopératoire immédiat et d’autre part, l’évolution fonctionnelle à long terme. Conclusions : L’implantation d’AHE est bien toléré. Les résultats fonctionnels immédiats chez les patients bénéficiant de l’implantation d’AHE sont excellents et superposables au groupe témoin. Les patients implantés démontrent un continuum évolutif, à long terme, de différentes caractéristiques vocales et laryngées, bien au-delà de la phase postopératoire immédiate. A l’inverse, les patients du groupe contrôle, non implanté, n’évoluent plus au-delà de la phase post-opératoire immédiate. [less ▲]

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See detailLa microchirurgie cordale, chez qui et comment?
FINCK, Camille ULg

Scientific conference (2002, December)

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See detailLa microchirurgie cordale
FINCK, Camille ULg

Scientific conference (2007, May)

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See detailMicroclimat et composition de l'air dans une enceinte fermée destinée à l'étude de la toxicité des polluants atmosphériques chez le porcelet
Urbain, B.; Gustin, Pascal ULg; Prouvost, J. F. et al

in Veterinary Research (1993), 24(6), 503-514

An experimental model representing the pollution background in a pigpen was constructed in order to study the toxicology of pig respiratory air pollutants. Temperature, relative humidity, air-flow rate ... [more ▼]

An experimental model representing the pollution background in a pigpen was constructed in order to study the toxicology of pig respiratory air pollutants. Temperature, relative humidity, air-flow rate, total dust, total viable particles, endotoxins in respirable dust (< 5 microns) and ammonia were measured in a 1.9 m3 environmental chamber designed for piglets. The activity of 1 piglet was recorded on video. Temperature and relative humidity were respectively 23.9 +/- 1.3 degrees C and 70.5 +/- 8.3% (mean +/- SD). Air flow rate was 10 m3/h. Dust, viable particles, endotoxin and ammonia concentrations were respectively 7 +/- 2 particles/ml, 3.4 x 10(4) +/- 2.9 x 10(4) BCFP (bacterial colony-forming particles)/m3, 4.8 +/- 1.5 ng/m3 and 6 +/- 0.8 ppm (mean +/- SD). All these parameters were comparable to the lowest values recorded in pigpens [less ▲]

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See detailMicrocracks-induced damage modelling for transversely isotropic rock
Levasseur, Séverine ULg; Welemane, Hélène; Kondo, Djimédo

in 3rd International Symposium on Computational Geomechanics (ComGeo III) (2013)

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See detailMicrocrystalline cellulose, a direct compression binder in a quality by design environment—A review
Thoorens, Grégory; Krier, Fabrice ULg; Leclercq, Bruno et al

in International Journal of Pharmaceutics (2014), 473(1-2), 64-72

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See detailMicrocystin congener- and concentration-dependent induction of murine neuron apoptosis and neurite degeneration.
Feurstein, Daniel; Stemmer, Kerstin; Kleinteich, Julia ULg et al

in Toxicological sciences : an official journal of the Society of Toxicology (2011), 124(2), 424--31

Cyanobacterial microcystins (MCs) represent a toxin group with > 100 variants, requiring active uptake into cells via organic anion-transporting polypeptides, in order to irreversibly inhibit serine ... [more ▼]

Cyanobacterial microcystins (MCs) represent a toxin group with > 100 variants, requiring active uptake into cells via organic anion-transporting polypeptides, in order to irreversibly inhibit serine/threonine-specific protein phosphatases. MCs are a human health hazard with repeated occurrences of severe poisonings. In the well-known human MC intoxication in Caruaru, Brazil (1996), patients developed signs of acute neurotoxicity, e.g., deafness, tinnitus, and intermittent blindness, as well as subsequent hepatotoxicity. The latter data, in conjunction with some animal studies, suggest that MCs are potent neurotoxins. However, there is little data to date demonstrating MC neuron-specific toxicity. MC exposure-induced cytotoxicity, caspase activity, chromatin condensation, and microtubule-associated Tau protein hyperphosphorylation (epitopes serine199/202 and serine396) were determined. Neurite degeneration was analyzed with confocal microscopy and neurite length determined using image analysis. MC-induced apoptosis was significantly increased by MC-LF and MC-LW, however, only at high concentrations (≥ 3$\mu$M), whereas significant neurite degeneration was already observed at 0.5$\mu$M MC-LF. Moreover, sustained hyperphosphorylation of Tau was observed with all MC congeners. The concentration- and congener-dependent mechanisms observed suggest that low concentrations of MC-LF and MC-LW can induce subtle neurodegenerative effects, reminiscent of Alzheimer's disease type human tauopathies, and thus should be taken more seriously with regard to potential human health effects than the apical cytotoxicity (apoptosis or necrosis) demonstrated at high MC concentrations. [less ▲]

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See detailLe microdamier d’expression : un outil performant dans la compréhension de la réponse
Schoeneker, Julie; Art, Tatiana ULg; Pirottin, Dimitri ULg et al

in Annales de Médecine Vétérinaire (2011), 154

Malgré de multiples études, les interrelations entre l’exercice, le stress et la réponse immune sont peu définies. Or, la compréhension de ces interrelations pourrait jouer un rôle très important dans ... [more ▼]

Malgré de multiples études, les interrelations entre l’exercice, le stress et la réponse immune sont peu définies. Or, la compréhension de ces interrelations pourrait jouer un rôle très important dans l’amélioration de la santé et des résultats sportifs des athlètes. En effet, l’exercice peut être reconnu comme un stress. Il induit des modifications de l’équilibre homéostatique qui peuvent à leur tour altérer la réponse immunitaire de l’hôte et donc sa susceptibilité aux maladies. L’adrénaline est la molécule essentielle de tout processus de stress. La technologie des microdamiers, outil majeur d’investigation transcriptomique, permet l’étude de l’expression génique de l’ensemble du génome. Son utilisation devrait donc permettre de mieux caractériser et définir les interrelations entre l’exercice, le stress et la réponse immune. Cette revue recense les interrelations connues entre la réponse immune à un stress adrénergique d’une part et la réponse immune à l’exercice d’autre part. Elle considère en outre la contribution potentielle des microdamiers à une meilleure compréhension des effets d’un stress, et plus particulièrement celui lié à l’exercice, sur l’immunité. [less ▲]

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See detailLe microdamier: un outil du futur pour le diagnostic de l’inflammation ?
Mignot, Clémence ULg; Fraipont, Audrey ULg; Richard, Eric et al

Poster (2009)

Detailed reference viewed: 49 (19 ULg)