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See detailA lysine cluster in domain II of Bacillus subtilis PBP4a plays a role in the membrane attachment of this C1-PBP
Vanden Broeck, Arnaud; Van Der Heiden, Edwige ULg; Sauvage, Eric ULg et al

in PLoS ONE (2015)

In PBP4a, a Bacillus subtilis class-C1 penicillin-binding protein (PBP), four clustered lysine (K) residues, K86, K114, K119, and K265, protrude from domain II. Replacement of these amino acids with ... [more ▼]

In PBP4a, a Bacillus subtilis class-C1 penicillin-binding protein (PBP), four clustered lysine (K) residues, K86, K114, K119, and K265, protrude from domain II. Replacement of these amino acids with glutamine (Q) residues by site-directed mutagenesis yielded Mut4KQ PBP4a. When produced in Escherichia coli without its predicted Sec-signal peptide, wildtype (WT) PBP4a was found mainly associated with the host cytoplasmic membrane, whereas Mut4KQ PBP4a remained largely unbound. After purification, the capacities of the two proteins to bind to B. subtilis membranes were compared. The results were similar to those obtained in E. coli: in vitro, a much higher percentage of WT PBP4a than of Mut4KQ PBP4a was found to interact with B. subtilis membranes. Immunodetection of PBP4a in B. subtilis membrane extracts revealed that a processed form of this PBP (as indicated by its size) associates with the B. subtilis cytoplasmic membrane. In the absence of any amphiphilic peptide in PBP4a, the crown of positive charges on the surface of domain II is likely responsible for the cellular localization of this PBP and its attachment to the cytoplasmic membrane. [less ▲]

Detailed reference viewed: 39 (14 ULg)
See detailLysosomal phospholipases inhibition by emetine, strychnopentamine and usambarensine
Quetin-Leclercq, Joelle; Schomer, G; Van Bambeke, F et al

Poster (1999)

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See detailLysozyme gene activity in chicken macrophages is controlled by positive and negative regulatory elements.
Steiner, Christoph; Muller, Marc ULg; Baniahmad, Aria et al

in Nucleic Acids Research (1987), 15(10), 4163-78

The chicken lysozyme gene is constitutively active in macrophages and under the control of steroid hormones in the oviduct. To investigate which DNA elements are involved in the control of its expression ... [more ▼]

The chicken lysozyme gene is constitutively active in macrophages and under the control of steroid hormones in the oviduct. To investigate which DNA elements are involved in the control of its expression in macrophages we performed transient DNA transfer experiments with two different types of plasmids: 5'-deletion mutants of the upstream region of the chicken lysozyme gene and different fragments from this area in front of the thymidine kinase promoter (herpes simplex virus), each placed in front of the CAT (chloramphenicol acetyl transferase) coding sequence. Two enhancers (E-2.7 kb and E-0.2 kb) were characterized. They are active in macrophages, but not in chicken fibroblasts. Furthermore a negative element (N-2.4 kb) was identified, which is active in fibroblasts and promyelocytes, but not in mature macrophages. The combined action of all three elements contributes to the observed lysozyme gene activities: no activity in fibroblasts, moderate activity in promyelocytes and high activity in mature macrophages. [less ▲]

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See detailLʼétalement urbain en Europe : un défi environnemental ignoré
Halleux, Jean-Marie ULg

Article for general public (2007)

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See detailLʼimmobilier de bureau en Belgique, un révélateur des mutations économiques et urbaines ?
Bruck, L.; Mairy, N.; Halleux, Jean-Marie ULg et al

in Bulletin de la Société Géographique de Liège (2005), 46

The aim of the paper is to analyse how office markets are in interaction with the evolutions in the field of territory and economy. The first part of the paper analyses the data available to study the ... [more ▼]

The aim of the paper is to analyse how office markets are in interaction with the evolutions in the field of territory and economy. The first part of the paper analyses the data available to study the office stocks. Afterwards, we present an urban hierarchy based on the office equipment, in relation with the process of metropolization and its location consequences. The last part of the paper shows that the office function is also concerned with the process of periurbanization. [less ▲]

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See detailM Cushing : a life long experience with pituitary surgery
Beckers, Albert ULg

Scientific conference (2008, October 07)

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See detailM- Service Value Perceptions and Customer Loyalty
Pihlstrom, M.; Liljander, V.; Van Riel, Allard ULg

Poster (2008, July 14)

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See detailM-Ingestion: Simultaneous Ingestion of Ionosonde and GNSS Data into the NeQuick Model
Bidaine, Benoît ULg; Nava, Bruno; Stankov, Stanimir et al

in Doherty, P.; Hernández-Pajares, M.; Juan, J.-M. (Eds.) et al The International Beacon Satellite Symposium BSS2010 (2010, June)

The steady increase in volume and quality of ionospheric measurements allows for potential improvements in the existing ionospheric models. In particular ionosonde and Global Navigation Satellite Systems ... [more ▼]

The steady increase in volume and quality of ionospheric measurements allows for potential improvements in the existing ionospheric models. In particular ionosonde and Global Navigation Satellite Systems (GNSS) data provide complementary information for original developments regarding the NeQuick model, a quick-run empirical model of the electron density supplying flexible solutions for various applications. Indeed NeQuick is often used to investigate radiowave propagation issues: among others it has been implemented within the Galileo Single Frequency Ionospheric Correction Algorithm. The model is particularly suited to be used within an optimization procedure called ingestion. In this framework, an "effective ionization level" Az plays the role of solar activity input in order to fit a specific dataset eg of slant Total Electron Content (TEC). However larger discrepancies can then obviously be observed towards other parameters eg the F2-layer critical frequency foF2. This draws the interest for a multiple ingestion - or m-ingestion - scheme implying an adaptation towards several datasets by defining several effective parameters. In our study, we perform m-ingestion of ionosonde and GNSS data providing us with slant TEC. In fact it allows us to constrain the electron density profile slab thickness. For the purpose, a high solar activity period (year 2002) was analysed based on collocated digisonde and EUREF GNSS observations at a mid-latitude site, Dourbes (50.1◦N, 4.6◦E). In this way, we can gather some clues on expected ionospheric effects on Galileo applications during the next solar activity maximum. [less ▲]

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See detailM-N interaction in beam-to-column joints - Development of a design model
Demonceau, Jean-François ULg; Jaspart, Jean-Pierre ULg

in Zingoni, Alphose (Ed.) Proceedings of the SEMC 2010 International Conference (2010, September 06)

The component method is a nowadays widely recognized procedure for the evaluation of the design properties of structural joints. It is used as a reference method in the Eurocodes and the proposed rules in ... [more ▼]

The component method is a nowadays widely recognized procedure for the evaluation of the design properties of structural joints. It is used as a reference method in the Eurocodes and the proposed rules in these codes are mainly devoted to the characterization of joints subjected to bending moments and shear forces. However, in some situations, these joints can be subjected to combined axial loads and bending mo-ments, for instance in frames subjected to an exceptional event leading to the loss of a column, situation where significant tying forces can developed in the structural beams above the lost column. In this paper, a design model, founded on the component method, aiming at predicting the behaviour of joints subjected to com-bined axial loads and bending moments is presented. In particular, it is illustrated how this model was vali-dated through comparisons to recent experimental tests performed on steel-concrete composite beam-to-column joints. [less ▲]

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See detailM-type channels selectively control bursting in rat dopaminergic neurons
Drion, Guillaume ULg; Bonjean, Maxime; Waroux, Olivier ULg et al

in European Journal of Neuroscience (2010), 31

Detailed reference viewed: 127 (60 ULg)
See detailM. Migeotte
Biémont, Emile ULg

in Nouvelle Bibliographie Nationale, Académie Royale de Belgique (2001), 6

Detailed reference viewed: 23 (4 ULg)
See detailM. Pastor et l'histoire des papes
Kurth, Godefroid ULg

in XXe Siecle (1896)

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See detailM. Paul Heger et les instituts universitaires de Gand et de Liége
Fredericq, Léon ULg

Book published by Vaillant Carmanne (1891)

Detailed reference viewed: 28 (0 ULg)
See detailM. Stumpf: Thermodynamische Untersuchungen wäßriger Zweiphasensysteme mit Leitsalzen zur extraktiven Trennung von Biomolekülen
Gaube, J; Keil, B; Müller, A et al

in BIOforum (1994), 17

Detailed reference viewed: 6 (0 ULg)