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See detailA New Dimethyl Sulfoxide-Based Method for Gene Promoter Methylation Detection
Kholod, Natalia ULg; Boniver, Jacques ULg; Delvenne, Philippe ULg

in Journal of Molecular Diagnostics (2007), 9(5), 574-81

The identification of gene promoter methylation is a useful tool for the molecular diagnosis of human diseases. We have developed a new PCR-based technique for detecting the methylation status of CpG ... [more ▼]

The identification of gene promoter methylation is a useful tool for the molecular diagnosis of human diseases. We have developed a new PCR-based technique for detecting the methylation status of CpG islands of gene promoters. This new method, named methyl-sensitive dimethyl sulfoxide-PCR (Ms-DMSO-PCR), is based on the finding that methylated and unmethylated DNAs show a different sensitivity to the amount of DMSO used in the PCR reaction. For the amplification of methylated DNA, more DMSO is required in comparison to unmethylated DNA. This finding resulted in the development of a simple PCR screening of CpG islands with addition of DMSO in the range from 0 to 8% (v/v), and the same pair of primers is sufficient for distinguishing hyper- or hypomethylated gene promoters from normally methylated sequences. This new technique is a one-step procedure and does not require any modifications of DNA or expensive equipment. Therefore, Ms-DMSO-PCR has the potential to be widely used for clinical applications as well in basic research. [less ▲]

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See detailA New Discontinuous Galerkin Formulation for Kirchhoff-Love Shells
Noels, Ludovic ULg; Radovitzky, Raul

Conference (2007)

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See detailA New Discontinuous Galerkin Formulation for Non-Linear Elasticity
Noels, Ludovic ULg; Chandran, Ram B; Radovitzky, Raul

Conference (2006)

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See detailA new discontinuous Galerkin method for Kirchhoff-Love shells
Noels, Ludovic ULg; Radovitzky, Raúl

in Computer Methods in Applied Mechanics & Engineering (2008), 197

Discontinuous Galerkin methods (DG) have particular appeal in problems involving high-order derivatives since they provide a means of weakly enforcing the continuity of the unknown-field derivatives. This ... [more ▼]

Discontinuous Galerkin methods (DG) have particular appeal in problems involving high-order derivatives since they provide a means of weakly enforcing the continuity of the unknown-field derivatives. This paper proposes a new discontinuous Galerkin method for Kirchhoff–Love shells considering only the membrane and bending response. The proposed one-field method utilizes the weak enforcement in such a way that the displacements are the only unknowns, while the rotation continuity is weakly enforced. This work presents the formulation of the new discontinuous Galerkin method for linear elastic shells, demonstrates the consistency and stability of the proposed framework, and establishes the method’s convergence rate. After a description of the formulation implementation into a finite-element code, these properties are demonstrated on numerical applications. [less ▲]

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See detailA new discontinuous Galerkin method for non-linear Mechanics
Noels, Ludovic ULg; Radovitzky, Raúl

(2006)

A discontinuous Galerkin framework for large deformations of solids is established. The method is based on a general Hu-Washizu-de Veubeke functional allowing for displacement and stress discontinuities ... [more ▼]

A discontinuous Galerkin framework for large deformations of solids is established. The method is based on a general Hu-Washizu-de Veubeke functional allowing for displacement and stress discontinuities in the domain interior. The focus of this paper is on the treatment of large elasto-plastic deformations. The method is shown to possess the required numerical properties: consistency in the non-linear range and linearized stability. In order to demonstrate the versatility, accuracy and robustness of the method examples of application and convergence studies in three dimensions are provided. Further developments of the method will lead to applications where physical discontinuities must be modeled like fragmentation or failure. [less ▲]

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See detailNew disruption cassettes for rapid gene disruption and marker rescue in the yeast Yarrowia lipolytica
Fickers, Patrick ULg; Le Dall, M. T.; Gaillardin, C. et al

in Journal of Microbiological Methods (2003), 55(3), 727-737

Yarrowia lipolytica is one of the most extensively studied nonconventional yeasts. Unfortunately, few methods for gene disruption have been reported for this yeast, and all of them are time-consuming and ... [more ▼]

Yarrowia lipolytica is one of the most extensively studied nonconventional yeasts. Unfortunately, few methods for gene disruption have been reported for this yeast, and all of them are time-consuming and laborious. The functional analysis of unknown genes requires powerful disruption methods. Here, we describe such a new method for rapid gene disruption in Y lipolytica. This knockout system combines SEP method and the Cre-lox recombination system, facilitating efficient marker rescue. Versatility was increased by using both auxotrophic markers like ylURA3 and ylLEU2, as well as the antibiotic resistance marker hph. The hph marker, which confers resistance to hygromycin-B, allows gene disruption in a strain lacking any conventional auxothrophic marker. The disruption cassette was shown to integrate at the correct locus at an average frequency of 45%. Upon expression of Cre recombinase, the marker was excised at a frequency of 98%, by recombination between the two lox sites. This new method for gene disruption is an ideal tool for the functional analysis of gene families, or for creating large-scale mutant collections in general. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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See detailA new distribution of vegetation types and its inclusion in a global biosphere model
Warnand; François, Louis ULg; Nemry, B. et al

Poster (1995, August)

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See detailA new diterpene enone from the soldier beetle Cantharis livida (Coleoptera : Cantharidae)
Durvaux, Christophe; Laurent, Pascal ULg; Daloze, Désiré et al

in ARKIVOC (2007), 10

Cantharenone, a new diterpene structurally related to the prenylbisabolane skeleton, has been isolated from the beetle Cantharis livida (Cantharidae). Its structure has been determined on the basis of its ... [more ▼]

Cantharenone, a new diterpene structurally related to the prenylbisabolane skeleton, has been isolated from the beetle Cantharis livida (Cantharidae). Its structure has been determined on the basis of its spectral properties. This is the first report of such a type of diterpenoid from an insect. [less ▲]

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See detailNew diversity patterns of Triassic conodonts
Plasencia, Pablo; Martínez-Perez, Carlos; Cascales - Miñana, Borja ULg et al

Conference (2013, July)

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See detailNew drugs and nutraceuticals in the treatment of osteoarthritis
Henrotin, Yves ULg; Reginster, Jean-Yves ULg

in Cox Gad, Shayne (Ed.) Development of Therapeutic Agents Handbook (2012)

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See detailA new easy method for specific measurement of active myeloperoxidase in human biological fluids and tissue extracts
Franck, Thierry ULg; Kohnen, Stephan; Zouaoui Boudjeltia, Karim et al

in Talanta (2009), 80

The SIEFED (“Specific Immunological Extraction Followed by Enzymatic Detection”) method already developed for the specific detection of the activity of equine myeloperoxidase (MPO) was adapted for the ... [more ▼]

The SIEFED (“Specific Immunological Extraction Followed by Enzymatic Detection”) method already developed for the specific detection of the activity of equine myeloperoxidase (MPO) was adapted for the specific measurement of active human MPO in biological fluids or tissue extracts. [less ▲]

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See detailNew Economy, New Myths ? The American Media Industry in Perspective
Geuens, Geoffrey ULg

Scientific conference (2008, October 16)

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See detailNew efficient bicomponent organocatalysts for the chemical fixation of CO2 onto epoxides: a theoretical study
Alves, Margot ULg; Méreau, Raphaël; Grignard, Bruno ULg et al

Poster (2015, May 18)

Regarding the economic and environmental issues, valorising CO2 as a C1 feedstock for producing useful building blocks is seducing as it is a free and abundant waste resulting from the human activity ... [more ▼]

Regarding the economic and environmental issues, valorising CO2 as a C1 feedstock for producing useful building blocks is seducing as it is a free and abundant waste resulting from the human activity. Cyclic carbonates are useful compounds that found application as green solvents, electrolytes for lithium battery or monomers for polycarbonates or polyurethanes synthesis. These cyclocarbonates can be synthesized with a total atom economy by chemical fixation of CO2 onto epoxides using organometallic complexes or organocatalysts. To date, although this area of research has been the subject of many studies, the identification and development of (organo)catalysts highly efficient under mild experimental conditions still remains challenging. In this context, we developed a new organocatalytic platform based on the use of ammonium salts in combination with single or double hydrogen bond donor activators derived from fluorinated alcohols that showed unexpected booster effect. In this contribution, the ammonium/fluoroalcohol promoted CO2/propylene oxide coupling was investigated through detailed kinetic studies by IR spectroscopy under pressure and results were compared to the most efficient organocatalysts based on ammonium salts and (multi)phenolic derivatives that were reported in the literature. In order to finely understand the reaction mechanism, this study was completed by molecular modeling. DFT calculations showed that the addition of H-bond donors (HBD) with hexafluoroisopropanol functionalities modified the mechanism of the ammonium promoted coupling of CO2 with epoxides. HBDs dramatically decreased the epoxide ring-opening step barrier which highlights the key role of the fluorinated activators in stabilizing the intermediates and transitions states by inter- and intra-molecular hydrogen bonds. [less ▲]

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See detailA new elastic-visco-plastic damage model for annealed aluminium: theory, calibration and validation
Habraken, Anne ULg; Zhang, Lihong ULg; Wang, X. C. et al

in Benallal, A. (Ed.) Proceedings of the Continuous Damage and Fracture Symposium (2000)

This article describes an energy based, two variables, isotropic damage model merged with Bodner's elasto-visco-plastic model and implemented in a non-linear finite element code. The procedure to identify ... [more ▼]

This article describes an energy based, two variables, isotropic damage model merged with Bodner's elasto-visco-plastic model and implemented in a non-linear finite element code. The procedure to identify the parameters of the model and its experimental validation are presented and applied to an annealed aluminium. [less ▲]

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See detailNew emission lines in Mira Ceti
Swings, Polydore ULg

in Publications of the Astronomical Society of the Pacific [=PASP] (1940), 52

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See detailNew Energy Levels in Ne II deduced from FTS spectra in the Infrared Region (1800-9000 cm-1)
Quinet, Pascal ULg; Palmeri, P.; Biémont, Emile ULg

in Physica Scripta (1994), 49

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See detailA New Energy-Dissipative Momentum-Conserving Time Integration Scheme Using the Variational Updates Framework.
Noels, Ludovic ULg; Stainier, Laurent ULg; Ponthot, Jean-Philippe ULg

(2007)

Although they are the most widely used time integration algorithms for finite-element discretizations, algorithms based on the Newmark family are numerically stable only for linear models. To overcome ... [more ▼]

Although they are the most widely used time integration algorithms for finite-element discretizations, algorithms based on the Newmark family are numerically stable only for linear models. To overcome that drawback, researches have recently focused on time integration algorithms stable in the non-linear range, leading to the development of Energy Momentum Conserving Algorithms or EMCA. A new Energy Momentum Conserving Algorithm was recently presented by the authors for elasto-plastic material models. This algorithm is based on a variational formulation of elasto-plastic updates for which the stress tensor always derives from an incremental potential, even when plastic behavior is considered. In this work this formalism is used to develop Energy-Dissipative Momentum-Conserving time integration algorithms, which have property of being stable in non-linear range, but also dissipate high-frequency numerical modes to improve the convergence properties. Using the potential existence of the variational updates framework, the verification of the numerical dissipation of energy in the non-linear range can easily be demonstrated. [less ▲]

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See detailA new ent-clerodane diterpenoid from Crassocephalum bauchiense Huch. (Asteraceae).
Tchinda, Alembert T.; Mouokeu, Simplice R.; Ngono, Rosalie A. N. et al

in Natural product research (2015)

A phytochemical investigation of the whole plant of Crassocephalum bauchiense Huch. resulted in the isolation of a new clerodane diterpenoid, ent-2beta,18,19-trihydroxycleroda-3,13-dien-16,15-olide (1 ... [more ▼]

A phytochemical investigation of the whole plant of Crassocephalum bauchiense Huch. resulted in the isolation of a new clerodane diterpenoid, ent-2beta,18,19-trihydroxycleroda-3,13-dien-16,15-olide (1), together with two known flavonoids 3',5-dihydroxy-4',5',6,7,8-pentamethoxyflavone (2) and 4',5-dihydroxy-3',5',6,7,8-pentamethoxyflavone (3). The compounds were tested against the chloroquine-sensitive 3D7 strain of Plasmodium falciparum. Compound 2 showed weak activity (IC50 = 10.1 g/mL) whilst compounds 1 and 3 were inactive. The structures of the compounds were elucidated by using detailed spectral analyses, especially 1H and 13C NMR, 1H-1H COSY, NOESY, HMBC and HR-ESI-MS. [less ▲]

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See detailA New Equilibrium Cell with Variable Cell Volume for Static Vapour-Pressure Measurements
Gaube, J; Hammer, S; Pfennig, Andreas ULg

in Fluid Phase Equilibria (1996), 123((1-2)), 245-257

Detailed reference viewed: 4 (0 ULg)