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Peer Reviewed
See detailLong term catheter-induced pulmonary endarteritis and right ventricular failure in a horse
Amory, Hélène ULg; Christmann, U.; Busoni, Valeria ULg et al

in Proceedings of the 39th Annual Congress of the British Equine Veterinairy Association (2000)

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See detailLong term clinical experience with glucosamine sulfate long-term clinical trials
Bruyère, Olivier ULg

Conference (2003, October 18)

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See detailLong term culture and characterization of avian primordial germ cells
Waroux, Olivier ULg; Tonus, Céline ULg; Grobet, Luc ULg

Conference (2013, October 26)

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See detailLong term culture and characterization of chicken primordial germ cells
Tonus, Céline ULg; Cloquette, Karine; Ectors, Fabien ULg et al

Poster (2013, October)

Avian primordial germ cells (PGCs) can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species ... [more ▼]

Avian primordial germ cells (PGCs) can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species. We have developed original methods that allow long term (20 month) expansion of primary cultures of undifferentiated PGCs and their efficient cryopreservation. Blood samples were collected from stage 13-18 embryos, pooled, deposited in cell culture inserts and co-cultivated in the presence of irradiated BRL cells. This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of undifferentiated PGCs lines. Overall, 35% of blood samples gave rise to PGCs cell lines originating from three commercial layer breeds and two Belgian endangered breeds. Moreover, we recently isolate and cultivate a new PGC line from turkey. All PGCs lines were first characterised for the expression of the stem cells and PGCs characteristic marker SSEA-1 by FACS. RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. In addition, by means of a quantitative PCR amplification of a chromosome W specific sequence, we demonstrated a progressive drift of all our lines towards the male sex (WL), while they were initially isolated from pooled blood samples with statistically equivalent numbers of male and female embryos (35 females: 29 males). PGCs were subsequently efficiently cryopreserved by slow freezing or by a newly developed vitrification method. Labelled PGCs from 10 lines were injected in recipient embryos. At day 6, colonization of the genital ridges confirmed that PGCs retain their gonadal migratory ability, both after long-term culture (min 3, max 20 month) and after cryopreservation. In order to evaluate the germinal differentiation of cultured PGCs during the gonadal development as well as the germline transmission rate, we established a stably expressing GFP line that was successfully injected in emrbyos. Results are in progress. [less ▲]

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See detailLong term culture and characterization of chicken primordial germ cells
Tonus, Céline ULg; Waroux, Olivier ULg; Cloquette, Karine et al

Poster (2012, November)

Avian primordial germ cells (PGCs), can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species ... [more ▼]

Avian primordial germ cells (PGCs), can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species. We have developed original methods that allow long term (20 month) expansion of primary cultures of undifferentiated PGCs and their efficient cryopreservation. Blood samples were collected from stage 13-18 embryos, pooled, deposited in cell culture inserts and co-cultivated in the presence of irradiated BRL cells. This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of undifferentiated PGCs lines. Overall, 35% of blood samples gave rise to PGCs cell lines originating from three commercial layer breeds and two Belgian endangered breeds. PGCs lines were first characterised for the expression of the stem cells and PGCs characteristic marker SSEA-1 by FACS (expression rate: 90-99%). RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. In addition, by means of a quantitative PCR amplification of a chromosome W specific sequence, we demonstrated a drift of all our lines towards the male sex (WL), while they were initially isolated from pooled blood samples with statistically equivalent numbers of male and female embryos (35 females: 29 males). PGCs were subsequently efficiently cryopreserved by slow freezing or by a newly developed vitrification method. Labelled PGCs from 10 lines were injected in recipient embryos. Colonization of the genital ridges confirmed that PGCs retain their gonadal migratory ability, both after long-term culture (min 3, max 20 month) and after cryopreservation. [less ▲]

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See detailLong term culture, cryopreservation and genetic modification of chicken primordial germ cells
Tonus, Céline ULg; Garcia Gil, Francisco José ULg; Cloquette, Karine et al

Poster (2015, October 16)

Avian primordial germ cells (PGCs) are precursor of gametes and appear during early stages of embryonic development. Under appropriate culture conditions, these cells can keep their germ cells properties ... [more ▼]

Avian primordial germ cells (PGCs) are precursor of gametes and appear during early stages of embryonic development. Under appropriate culture conditions, these cells can keep their germ cells properties in vitro and are foreseen as promising tools for developing efficient avian genetic engineering and preservation of germplasm. We propose original methods that allow long term expansion, efficient cryopreservation and genetic modification of primary cultures of undifferentiated PGCs. PGCs are collected from embryonic blood during their migratory period and grown in cell-culture insert in the presence of feeder cells (BRL). This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of PGCs lines. Forty percent of blood samples gave rise to lines originating from three commercial layer and two Belgian endangered breeds. PGCs lines were characterized for the expression of the stem cells and PGCs marker SSEA-1 by FACS. RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. All lines were male although isolated from pooled male and female blood samples. Two cryopreservation methods were developed based upon slow-freezing and aseptic vitrification. Both have shown a similar effectiveness in allowing storage without phenotype drift. Stably expressing lines were obtained by Lipofectamine® mediated transfection of a GFP plasmid. PGCs were subsequently injected in recipient embryos. Persistence of exogenous PGCs in the developing gonad of recipient embryos confirmed that PGCs retain their gonadal colonisation ability, both after long-term culture and after cryopreservation. [less ▲]

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See detailLong term dynamics of carabid beetles in Belgium : a preliminary analysis on the influence of changing climate and land use by means of a database covering more than a century.
Desender, Konjev; Dufrêne, Marc ULg; Maelfait, Jean-Pierre

in Desender, Konjev; Dufrêne, Marc; Loreau, Michel (Eds.) et al Carabid Beetles Ecology and Evolution (1994)

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See detailLong term efficacy of strontium ranelate in reducing the risk of vertebral and non-vertebral including hip fractures in post menopausal osteoporotic women over 5 years
Reginster, Jean-Yves ULg; Brixen, Kim; Cormier, C. et al

in Annals of the Rheumatic Diseases (2007, June), 66(Suppl.II), 102

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See detailLong term evolution and impact of immunomodulator cotreatment and withdrawal on infliximab on trough levels in 223 patients with Crohn's disease
Drobne, D; Bossuyt, P; Breynaert, C et al

in Journal of Crohn’s and Colitis [=JCC] (2011)

Detailed reference viewed: 42 (1 ULg)
See detailLong term evolution of the seagrass bed in Calvi, and carbon cycling in the ecosystem
Soullard, Mady; Bouquegneau, Jean-Marie ULg; Dauby, Patrick ULg et al

Conference (1994, January)

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See detailLong term follow-up of patients with acute myelogenous leukemia who received the daunorubicin, vincristine, and cytosine arabinoside regimen.
Beguin, Yves ULg; Sautois, Brieuc ULg; Forget, Patricia ULg et al

in Cancer (1997), 79(7), 1351-4

BACKGROUND: In 1985, the authors published a study of acute myelogenous leukemia (AML) patients treated with a chemotherapeutic regimen that was then considered intensive. Ten years later, the authors ... [more ▼]

BACKGROUND: In 1985, the authors published a study of acute myelogenous leukemia (AML) patients treated with a chemotherapeutic regimen that was then considered intensive. Ten years later, the authors reanalyzed the same cohort to determine whether the very promising actuarial results observed at 5 years held after longer follow-up. METHODS: Between 1977 and 1982, 61 patients with AML were treated with a protocol consisting of daunorubicin, vincristine, and cytosine arabinoside induction followed by consolidation and maintenance for a total of 2 years. The complete remission (CR) rate was 66%, 84% in males versus 47% in females (P < 0.005). At the time of the first analysis in 1984, the overall survival (OS) was 17%, the projected 5-year continuous CR rate (CCR) 32%, and the disease free survival (DFS) rate 29%, with the best results observed for males and for patients ages 40-60 years (P < 0.05). RESULTS: When the data were reanalyzed 11 years later in 1995, the results were 14% OS, 23% CCR, and 16% DFS at 5 years. However, these figures dropped to 8%, 18%, and 11% at 10 years and to 8%, 12%, and 7% at 15 years, respectively. Among the 40 CR patients, 31 relapsed (up to 13 years after CR), and all died within 1.6 years after relapse. Nine patients were in CCR: 4 died of unrelated causes (suicide, alcoholic cirrhosis, acute peritonitis, or bladder carcinoma), 1 was lost to follow-up after 11 years, 2 were alive and well at 17 years at last follow-up, and 2 were transplanted in first CR and were doing well at 13 and 14 years at last follow-up. The survival advantage for males over females persisted (P = 0.0197), but the advantage for patients age 40-60 years did not hold. CONCLUSIONS: These long term data indicate that actuarial analysis at 5 years may overestimate the cure rate of AML patients because a number of late relapses do occur. However, the picture is blurred by the incidence of death not related to leukemia or its treatment; and when these patients were censored at the time of death, 17% of CR patients were still projected to be alive and free of leukemia after 17 years. [less ▲]

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See detailLong term follow-up study of occipital nerve stimulation (ONS) for refractory chronic cluster headache: drastic change from short term outcome
Magis, Delphine ULg; Remacle, J. M.; Schoenen, Jean ULg

in Cephalalgia : An International Journal of Headache (2006, November), 26(11), 1398

Detailed reference viewed: 29 (3 ULg)
Peer Reviewed
See detailLong Term household waste management : Insignhts from the policymaking process
Bakkour, Darine; Berthomé, Karim; Mormont, Marc ULg et al

Conference (2013, July 05)

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See detailLong term Immune Reconstitution and infection burden after Mismatched Hematopoietic Stem Cell Transplantation
SERVAIS, Sophie ULg; Lengline, Etienne; Porcher, Raphael et al

in Biology of Blood & Marrow Transplantation (2014), 20(4), 507-517

Mismatched unrelated donor (MMUD) or umbilical cord blood (UCB) can be chosen as alternative donors for allogeneic stem cell transplantation but might be associated with long lasting immune deficiency ... [more ▼]

Mismatched unrelated donor (MMUD) or umbilical cord blood (UCB) can be chosen as alternative donors for allogeneic stem cell transplantation but might be associated with long lasting immune deficiency. Sixty-six patients who underwent a first transplantation from either UCB or 9/10 MMUD (n= 36) and who survived beyond 3 months were evaluated. Immune reconstitution was prospectively assessed at sequential time points after transplantation. NK, B, CD4+ and CD8+T cells and their subsets as well as regulatory T cells (Treg) were studied. Detailed analyses on infections occurring after 3 months were also assessed. The 18-month cumulative incidences of infection-related death were 8 and 3%, and of infections were 72 and 57% after MMUD and UCB transplantation, respectively. Rates of infection per 12 patient-month were roughly 2 overall (1 for bacterial, 0.9 for viral and 0.3 for fungal infections). Memory, naïve CD4+ and CD8+T cells, naïve B cells and Treg cells reconstitution between the 2 sources was roughly similar. Absolute CD4+T cells hardly reached 500 per μL by one year posttransplantation and most B cells were of naïve phenotype. Correlations between immune reconstitution and infection were then performed by multivariate analyses. Low CD4+ and high CD8+T cells absolute counts at 3 months were linked to increased risks of overall and viral (but not bacterial) infections. When assessing for the naïve/memory phenotypes at 3 months among the CD4+ T cell compartment, higher percentages of memory subsets were protective against late infections: central memory CD4+T cells protected against overall and bacterial infections; late effector memory CD4+T cells protected against overall, bacterial and viral infections. At the opposite, high percentage of effector- and late effector-memory subsets at 3 months among the CD8+ T cell compartment predicted higher risks for viral infections. Patients transplanted from alternative donors represent a population with very high risk of infection. Detailed phenotypic analysis of immune reconstitution may help to evaluate infection risk and to adjust infection prophylaxis. [less ▲]

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See detailLong term measurements of VOC exchanges above a maize field at Lonzée (Belgium)
Bachy, Aurélie ULg; Aubinet, Marc ULg; SALERNO, Giovanni ULg et al

Poster (2013, June 10)

For the last decades, VOC had arisen scientifict interest due to their important role in the atmospheric chemistry and their final impact on air pollution and climate change. Terrestrial ecosystems being ... [more ▼]

For the last decades, VOC had arisen scientifict interest due to their important role in the atmospheric chemistry and their final impact on air pollution and climate change. Terrestrial ecosystems being the main VOC source, evaluation of current and future biogenic VOC emissions through VOC exchange modeling is thus necessary to better estimate future climate and assess future air pollution risks. BVOC exchanges depend on edaphic variables and are plant species specific. Therefore, their modeling and global budget evaluation requires a comprehensive understanding of production and exchange dynamics under a wide panel of climatic conditions and ecosystems, which necesserily implies BVOC exchange measurements under varied conditions. In that perspective, forest and non pastured grasslands have been largely studied for the last decade, but knowledge about BVOC fluxes from croplands remains still scarce. As a consequence, crop species-specific standard emissions that feed bottom-up BVOC emission models are still often assigned to a default value that is in addition kept constant for the entire growth season, although recent research has shown that plant phenology, acclimation and stress can drastically influence BVOC emissions. To help filling this knowledge gap, we run a project that aims to study VOC fluxes from two major croplands, maize (2nd most important culture worldwide) and winter wheat (1st most important culture worldwide), and a pastured grassland. We present here a specific study focussing on the VOC exchanges between a maize field and the atmosphere. VOC fluxes were measured at ecosystem-scale during the whole 2012 growing season using the eddy covariance by mass-scaning technique with a proton-transfer-reaction mass spectrometer. Together with VOC fluxes, we also recorded a wide set of ancillary data including CO2 fluxes, meteorological variables and biomass evolution. As far as we know, we are the first study dealing with BVOC measurements on maize at ecosystem scale and spanning all the phenological stages of the crop. Although first results show half-hourly bidirectionnal exchanges among all the preselected compounds, in average methanol is the greatest emitted VOC, followed by green leaf volatiles. Acetic acid and acetaldehyde are the greatest taken up VOC. Small isoprene and monoterpene fluxes are also observed. A diurnal pattern is found for all those VOC, with greater emission/uptake during the day, suggesting a flux dependence on environmental parameters. Influence of environmental controls, biomass evolution (including growth primary production) and phenology on fluxes is currently under investigation. Our research allows to quantify BVOC exchanges by a maize field throughout a whole growing season. Hence, obtained results will refine the understanding of the BVOC exchanges mechanisms by including both environmental and phenological parameters. Such results are expected to be very useful for BVOC modeling, especially for oxygenated compounds such as methanol. [less ▲]

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See detailLong term measurements of volatile organic compounds exchanges above a maize field at Lonzee (Belgium)
Bachy, Aurélie ULg; Aubinet, Marc ULg; SALERNO, Giovanni ULg et al

in Communications in Agricultural and Applied Biological Sciences (2013, February), 78(1), 127-132

VOC (volatile organic compounds) include a wide set of molecules which are mostly emitted by the plants. Atmospheric scientists are strongly interested in these compounds because of their important role ... [more ▼]

VOC (volatile organic compounds) include a wide set of molecules which are mostly emitted by the plants. Atmospheric scientists are strongly interested in these compounds because of their important role in the atmospheric chemistry and their final impact on air pollution and climate change. Evaluation of current and future VOC emissions is thus necessary and requires a comprehensive understanding of VOC production and exchange dynamics under a wide panel of climatic conditions and ecosystems. Forest and non pastured grasslands have been largely studied for the last decade. However, knowledge about VOC fluxes from croplands remains scarce. Our study focuses on the VOC exchanges between a maize field and the atmosphere. It is incorporated in a wider project that aims to study VOC fluxes from two croplands (maize and winter wheat) and a pastured grassland. VOC fluxes have been measured on a maize field during the whole growing season using a micrometeorological method (eddy covariance). While first results show half-hourly bidirectionnal exchanges among all the preselected compounds, in average methanol stands for the greatest emitted VOC, followed by green leaf volatiles, and acetic acid is the greatest taken up VOC. Small isoprene and monoterpenes fluxes are also observed. A diurnal pattern is found for all those VOC, with greater emission/uptake during the day, suggesting a flux dependence on environmental parameters. These environmental controls will be further investigated [less ▲]

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See detailLong term mobilisation of chemical elements in tephra-rich peat (NE Iceland)
De Vleeschouwer, François ULg; Lanoe, Brigitte Van Vliet; Fagel, Nathalie ULg

in Applied Geochemistry (2008), 23(12), 3819-3839

This paper presents geochemical profiles of a tephra-bearing minerotrophic peat column from NE-Iceland obtained using various elemental analyses of the solid phase and the pore water. The influence of ... [more ▼]

This paper presents geochemical profiles of a tephra-bearing minerotrophic peat column from NE-Iceland obtained using various elemental analyses of the solid phase and the pore water. The influence of tephra grain size, thickness and composition of each tephra on the peat geochemistry was investigated. Interpretations are supported by a statistical approach, in particular by autocorrelation, and by microscopy observations. Minerotrophic peat geochemistry may be strongly dependent upon post-depositional mobilization and possible leaching of elements as demonstrated by Fe and trace metal concentration profiles. Chemical elements, and more specifically potentially harmful metals, can be slowly leached out of volcanic falls during their weathering and re-accumulate downwards. It is emphasised that a tephra deposit can act as an active geochemical barrier, blocking downward elemental movements and leading to the formation of enriched layers. In this study, the formation of poorly amorphous Fe phases above the Hekla 3 tephra is shown. These poorly crystalline Fe phases scavenged Ni. (c) 2008 Elsevier Ltd. All rights reserved. [less ▲]

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See detailLong term observations of carbon dioxide exchange over cultivated savanna under a Sudanian climate in Benin (West Africa)
Ago, Expédit Evariste ULg; Agbossou, Euloge Kossi; Galle, Sylvie et al

in Agricultural and Forest Meteorology (2014), 197

Turbulent CO2 exchanges between a cultivated Sudanian savanna and the atmosphere were measured during 29 months (August 2007–December 2009) by an eddy-covariance system in North-Western Benin, West Africa ... [more ▼]

Turbulent CO2 exchanges between a cultivated Sudanian savanna and the atmosphere were measured during 29 months (August 2007–December 2009) by an eddy-covariance system in North-Western Benin, West Africa. The site (Lat 9.74◦ N, Long 1.60◦ E, Alt: 449 m) is the one of three sites fitted out by the international AMMA-CATCH program. The flux station footprint area is mainly composed of herbs and crops with some sparse trees and shrubs. Fluxes data were completed by an inventory of dominating species around the tower and the meteorological measurements. Flux response to climatic and edaphic factors was studied. Water was found the main controlling factor of ecosystem dynamics: much larger uptake was found in wet than dry season. During wet season, a very clear answer of net CO2 fluxes to photosynthetic photon fluxes density (PPFD) was observed. A low limitation in response to saturation deficit and soil water variability was however observed. The total ecosystem respiration (TER) was found highly dependent on soil moisture below 0.1 m3m−3, but saturates above this threshold. The average annual carbon sequestration was 232 ± 27 gC m−2 with its inter-annual variability mainly controlled by TER. Finally, the ecosystem appeared more efficient during morning and wet season than during afternoon and dry period. [less ▲]

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See detailLong term outcome in dogs with sinonasal aspergillosis treated with intranasal infusion of enilconazole
Schuller, S.; Clercx, Cécile ULg

in 14th ESVIM Meeting - Barcelona - Espagne (2004, September)

Detailed reference viewed: 17 (0 ULg)