Relationship between propeptide pH unfolding and inhibitory ability during ProDer p 1 activation mechanism

in Journal of Molecular Biology (2007), 374(1), 170-185

The major allergen Der p1 of the house dust mite Dermatophagoides pteronyssinus is a papain-like cysteine protease (CA1) produced as an inactive precursor and associated with allergic diseases. The ... [more ▼]

The major allergen Der p1 of the house dust mite Dermatophagoides pteronyssinus is a papain-like cysteine protease (CA1) produced as an inactive precursor and associated with allergic diseases. The propeptide of Der p I exhibits a specific fold that makes it unique in the CA1 propeptide family. In this study, we investigated the activation steps involved in the maturation of the recombinant protease Der p 1 expressed in Pichia pastoris and the interaction of the full-length and truncated soluble propepticles with their parent enzyme in terms of activity inhibition and BIAcore interaction analysis. According to our results, the activation of protease Der p 1 is a multistep mechanism that is characterized by at least two intermediates. The propeptide strongly inhibits unglycosylated and glycosylated recombinant Der p 1 (K-D = 7 nM) at neutral pH. This inhibition is pH dependent. It decreases from pH 7 to pH 4 and can be related to conformational changes of the propepticle characterized by an increase of its flexibility and formation of a molten globule state. Our results indicate that activation of the zymogen at pH 4 is a compromise between activity preservation and propeptide unfolding. (c) 2007 Elsevier Ltd. All rights reserved. [less ▲]

Relationship between sedimentary features and permeability at different scales in the Brussels Sands

in Geologica Belgica (2012), 15(3), 156-164

The Brussels Sands display a complex three-dimensional subsurface architecture. This sedimentological heterogeneity induces a highly heterogeneous spatial distribution of hydrogeological parameters at ... [more ▼]

The Brussels Sands display a complex three-dimensional subsurface architecture. This sedimentological heterogeneity induces a highly heterogeneous spatial distribution of hydrogeological parameters at different scales and may consequently influence subsurface fluid flow and solute migration. This study aims at characterizing spatial variability of permeability at different scales in the Brussels Sands. Firstly, a literature review on the permeability distribution of the Brussels Sands was performed. Secondly, a field campaign was carried out consisting of field observations of the small-scale sedimentary structures and in situ measurements of air permeability. A total of 6550 cm-scale air permeability measurements were carried out in situ in three Brussels Sands quarries in the central part of Belgium: Bierbeek, Mont Saint Guibert and Chaumont Gistoux. On the large basin scale, substantial differences in permeability are observed. A literature data analysis shows that there is no clear correlation between hydraulic conductivity and sedimentary facies. At the small scale, results show that permeability heterogeneity and anisotropy are strongly influenced by sedimentary heterogeneity in all three quarries. Clay-rich sedimentary features such as bottomsets and distinct mud drapes exhibit a different statistical and geostatistical permeability distribution compared to the cross-bedded lithofacies, where the permeability anisotropy is dominated by the foreset lamination orientation. [less ▲]

The relationship between serial order STM and vocabulary development: a longitudinal study.

in Developmental Psychology (2010), 46(2), 417-427

The relationship between short-term memory and language acquisition and development in Down syndrome

Conference (1995, April 27)

Some neuropsychological studies of brain damaged patients and studies of typical young children lead to the conclusion that the phonological short-term memory abilities directly influence the way new ... [more ▼]

Some neuropsychological studies of brain damaged patients and studies of typical young children lead to the conclusion that the phonological short-term memory abilities directly influence the way new vocabulary items are acquired. As Down’s syndrome people generally show an impairment of phonological short-term memory and a lack in lexical knowledge, we decided to study the relationship between both variables in this population. Our results indicate that in Down's syndrome subjects, as well as in typically developing children lexical knowledge seems to be linked to the phonological short-term memory abilities and more specially to nonword repetition. [less ▲]

Relationship between structural properties and affinity for herpes simplex virus type 1 thymidine kinase of bromine substituted 5-heteroaromatic 2'-deoxyuridines

in Antiviral Research (1996), 30

The crystal structures of 5-(5-furan-2-yl)-2'-deoxyuridine (II), 5-(5-bromofuran-2-yl)-2' deoxyuridine (IV) and 5-(3-bromothien-2-yl)-2'-deoxyuridine (V) have been studied in order to explain the ... [more ▼]

The crystal structures of 5-(5-furan-2-yl)-2'-deoxyuridine (II), 5-(5-bromofuran-2-yl)-2' deoxyuridine (IV) and 5-(3-bromothien-2-yl)-2'-deoxyuridine (V) have been studied in order to explain the different affinity of the compounds for the herpes simplex virus type 1 (HSV-1) thymidine kinase. These compounds present a variable affinity according to the position of the heteroatom substituting the five-membered ring. An unfavourable substitution in the five-membered ring for interaction with the HSV-1 thymidine kinase has been identified. [less ▲]

) Relationship between subaerial exposure features on a carbonate platform and reservoir properties (example of the Campo section, Spain, Paleocene).

in Abstract book 17th ISC - Fukuoka, Japan - 2006 (2006)

Relationship between subchondral tibial bone mineral density and joint space width at the medial femoro-tibial compartment in patients with knee osteoarthritis

in Clinical Rheumatology (2001), 5(Suppl.1), 411

Relationship between surface characteristics and superficial cohesion of concrete

in Beushausen, H.; Dehn, F.; Alexander, M. G. (Eds.) Concrete Repair, Rehabilitation and Retrofitting (2006)

Existing concrete surfaces have to be prepared before the application of a new layer. Different surface treatments (grinding, sandblasting, shotblasting, hand- and mechanical milling) have been performed ... [more ▼]

Existing concrete surfaces have to be prepared before the application of a new layer. Different surface treatments (grinding, sandblasting, shotblasting, hand- and mechanical milling) have been performed and the quality of the preparation has been established on the base of three main parameters: surface roughness, superficial concrete microcracking and adhesion. Cracks have been systematically observed, in relation with surface treatment by means of Scanning Electron Microscope and Light Microscope. Finally, a repair mortar, with or without bond coat, has been applied on the concrete substrates and adhesion has been measured. Analysis of results clearly show the effect of roughness on adhesion in the case where no bond coat is used, the influence of the surface treatment on the waviness shape of the profile and the presence of microcracks in the near-to-surface layer, which is related to failure type. [less ▲]

Relationship between the evolution of the shoreline and the Posidonia oceanica meadow limit in a Sardinian coastal zone

in Journal of Coastal Research (2007), 23(3), 787-793

Important environmental changes have been observed for some coastal processes in the Gulf of Oristano (west coast of Sardinia, Italy). With remote sensing as the principal tool, this study aims to assess ... [more ▼]

Important environmental changes have been observed for some coastal processes in the Gulf of Oristano (west coast of Sardinia, Italy). With remote sensing as the principal tool, this study aims to assess littoral evolution over time (1977-2000) and to evaluate whether there is a relationship between the evolutionary trend of the shoreline and that of the upper limit of the Posidonia oceanica (L.) Delile meadow. Results show that some portions of the coastline, mainly located on the sandy part of the littoral, have encountered significant variations that can be partially related to the evolution of the upper limit of the P. oceanica meadow. Mainly of natural origins, this evolutionary trend has also been related to anthropogenic pressures put on the seagrass meadow. This finding confirms that Posidonia meadows significantly affect the littoral geomorphology, providing biogenic sediments, controlling beach slope, and acting as a '' brake '' on coastal water masses. [less ▲]

Relationship between the protective activities of several strains of Trichoderma against damping-off agents and their ability to produce hydrolytic enzymes activities in soil or in synthetic media.

in Mededelingen van de Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen (Rijksuniversiteit te Gent) (1994), 59(3a),