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See detailOrganochlorine pollutants in sea turtles and their association with vitamin A
Dyc, Christelle ULg; Debier, Cathy; Thomé, Jean-Pierre ULg et al

Poster (2010, May)

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See detailOrganochlorine pollution in tropical rivers (Guadeloupe): Role of ecological factors in food web bioaccumulation
Coat, Sophie; Monti, Dominique; Legendre, Pierre et al

in Environmental Pollution (2011), 159

Concentrations of organochlorine pesticides and stable isotope ratios of nitrogen and carbon were measured in a tropical freshwater ecosystem to evaluate the contamination level of biota and examine the ... [more ▼]

Concentrations of organochlorine pesticides and stable isotope ratios of nitrogen and carbon were measured in a tropical freshwater ecosystem to evaluate the contamination level of biota and examine the bioaccumulation patterns of pollutants through the food web. Chemical analyses showed a general and heavy contamination of the entire food web. They revealed the strong accumulation of pollutants by juveniles of diadromous fishes and shrimps, as they re-enter the river. The role of ecological factors in the bioaccumulation of pesticides was evaluated. Whereas the most persistent pollutants (chlordecone and monohydro-chlordecone) were related to the organisms diet and habitat, bioaccumulation of b-HCH was only influenced by animal lipid content. The biomagnification potential of chlordecone through the food chain has been demonstrated. It highlighted the importance of trophic transfer in this compound bioaccumulation process. In contrast, bioconcentration by passive diffusion from water seemed to be the main exposure route of biota to b-HCH. [less ▲]

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See detailOrganochlorines and lactation : mobilization and transfer to offspring in northern elephant seals
Debier, Cathy; Crocker, D.; Xhonneux, V. et al

in Organohalogen Compounds (2007), 69

The transfer of PCBs and DDTs from mother to pup was studied in 20 Northern elephant seal Mirounga angustirostris mother-pup pairs. Organochlorine concentrations in milk increased from 127.3 ± 25.0 ng/g ... [more ▼]

The transfer of PCBs and DDTs from mother to pup was studied in 20 Northern elephant seal Mirounga angustirostris mother-pup pairs. Organochlorine concentrations in milk increased from 127.3 ± 25.0 ng/g to 200.9 ± 39.6 ng/g for PCBs and from 278.2 ± 92.4 ng/g to 494.9 ± 163.4 ng/g for DDTs between day 4 and day 21 of lactation. A similar longitudinal rise was observed in the serum of mothers (5.4 ± 0.8 to 7.7 ± 1.4 ng/ml for PCBs and 7.8 ± 2.4 to 11.9 ± 4.1 ng/ml for DDTs) and, to a lesser extend, in the serum of their pups (5.8 ± 1.4 to 6.1 ± 2.5 ng/ml for PCBs and 8.3 ± 2.5 to 9.9 ± 4.3 ng/ml for DDTs) between early and late lactation. The rise of contaminants in serum and milk might originate from the changes observed in maternal inner blubber in which PCB and DDT levels increased significantly throughout lactation (604.2 ± 142.8 to 1292.5 ± 421.6 ng/g lipids for PCBs and 1223.9 ± 460.0 to 2395.9 ± 737.3 ng/g lipids for DDTs). By contrast, contaminant levels remained constant in outer blubber (933.2 ± 271.8 to 999.7 ± 223.5 ng/g lipids for PCBs and 2010.7 ± 698.3 to 2061.9 ± 603.5 ng/g lipids for DDTs). [less ▲]

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See detailOrganoclays prepared in supercritical CO2: implication of onium stability on the properties of PA6 nanocomposites
Naveau, Elodie ULg; Calberg, Cédric ULg; Detrembleur, Christophe ULg et al

Conference (2009, June 23)

The organomodification of layered silicates via our patented supercritical CO2 ion-exchange process, enables the use of a large variety of surfactants, among which phosphonium and ammonium ions of the ... [more ▼]

The organomodification of layered silicates via our patented supercritical CO2 ion-exchange process, enables the use of a large variety of surfactants, among which phosphonium and ammonium ions of the very same structure. The as-obtained organoclays were melt blended with PA6 and the morphology as well as the fire properties of the nanocomposites were studied. With the same degree of nanodispersion, longer ignition times were observed with phosphonium-modified clays compared to ammonium-modified clays. [less ▲]

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See detailOrganometallic-mediated radical polymerization of vinyl amides: Effect of metal coordination
Kermagoret, Anthony ULg; Morin, A.; Hurtgen, Marie ULg et al

Poster (2012, September 04)

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See detailOrganometallic-mediated radical polymerization of vinyl amides: Effect of metal coordination
Kermagoret, Anthony ULg; Morin, A.; Hurtgen, Marie ULg et al

Conference (2012, September 04)

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See detailOrganometallic-mediated radical polymerization: unusual route toward (quasi-) diblock graft copolymers starting from a mixture of monomers of opposed reactivity
Hurtgen, Marie ULg; Debuigne, Antoine ULg; Fustin, Charles-André et al

in Macromolecules (2011), 44(12), 4623-4631

Graft copolymers have been prepared by one-step organometallic-mediated radical polymerization (OMRP) for the first time. Poly(ethylene glycol) acrylate (PEGA) was copolymerized with vinyl acetate (VAc ... [more ▼]

Graft copolymers have been prepared by one-step organometallic-mediated radical polymerization (OMRP) for the first time. Poly(ethylene glycol) acrylate (PEGA) was copolymerized with vinyl acetate (VAc) to yield well-defined P(PEGA-grad-VAc) gradient graft copolymers using bis(acetylacetonato)cobalt(II) as the control agent. The influence of experimental parameters such as the PEGA/VAc molar ratio, the nature of the initiator, and the temperature on the control of the copolymerization was discussed. The use of an excess of cobalt complex appeared as a key parameter to maintain a good level of control when higher contents of acrylate were used in the comonomer feed. The reactivity ratios were estimated and revealed that PEGA was added around 30 times faster than VAc, which gave access to a gradient P(PEGA-grad-VAc) copolymer or to a P(PEGA-grad-VAc)-b-PVAc diblock copolymer when the VAc polymerization was pursued after the full consumption of PEGA. The amphiphilic character of the copolymers makes them prone to self-assemble into micelles in water, as evidenced by dynamic light scattering. [less ▲]

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See detailOrganomodification of montmorillonite in supercritical carbon dioxide
Naveau, Elodie ULg; Calberg, Cédric ULg; Detrembleur, Christophe ULg et al

in Applied Clay Science (2011), 51(4), 467-772

The use of organo-montmorillonite as nanofiller for the preparation of polymer nanocomposites still attracts a lot of attention both on experimental and industrial scale. In order to enlarge the range of ... [more ▼]

The use of organo-montmorillonite as nanofiller for the preparation of polymer nanocomposites still attracts a lot of attention both on experimental and industrial scale. In order to enlarge the range of organo-montmorillonites available for this application, supercritical carbon dioxide (scCO2) was used as a medium for the organomodification process. This environmentally friendly solvent showed a great potential to obtain ready-to-use organo-montmorillonite powders. Intercalation with high degrees of exchange was obtained when the surfactants (alkyl ammonium, phosphonium, and imidazolium salts) were in the liquid state at the reaction temperature. The addition of a small quantity of co-solvent enabled the intercalation of solid surfactants, such as those bearing hydroxyl or carboxyl groups. Basal spacings of montmorillonite modified in scCO2 corresponded to those measured for organo-montmorillonite prepared in the wet process. A detailed investigation of the reaction conditions was finally performed in order to up-scale the process. [less ▲]

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See detailOrganon
Seron, Denis ULg

in Motte, André; Rutten, Christian (Eds.) Philosophie de la forme: Eidos, idea, morphé dans la philosophie grecque des origines à Aristote (2003)

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See detailOrganotypic Culture of HPV-Transformed Keratinocytes: A Model for Testing Lymphocyte Infiltration of (Pre)Neoplastic Lesions of the Uterine Cervix
Jacobs, Nathalie ULg; Moutschen, Michel P; Franzen-Detrooz, E. et al

in Virchows Archiv : An International Journal of Pathology (1998), 432(4), 323-30

The aim of our study was to establish the relevance of an in vitro model for analysing the ability of human lymphocytes to infiltrate human papillomavirus (HPV)-associated (pre)neoplastic lesions of the ... [more ▼]

The aim of our study was to establish the relevance of an in vitro model for analysing the ability of human lymphocytes to infiltrate human papillomavirus (HPV)-associated (pre)neoplastic lesions of the uterine cervix. To mimic these lesions, we have used the organotypic raft culture of HPV-transformed keratinocytes (SiHa). The SiHa organotypic raft culture was co-cultured with resting or prestimulated (IL-2 or IL-2+anti-CD3 mAb) allogeneic peripheral blood mononuclear cells (PBMC) for 24 and 72 h. The majority of infiltrating cells were T lymphocytes. Occasional NK cells were also identified. The stimulation with IL-2+anti-CD3 mAb induced the highest number of infiltrating cells, with the maximum lymphocyte infiltration observed after 24 h of co-culture. The lymphocyte infiltration was associated with an increased number of apoptotic cells in the organotypic cultures. The ability of PBMC and purified T cell and NK cell populations to lyse HPV-transformed keratinocytes was also investigated on monolayer cultures. As expected in an allogenic model, the highest cytotoxicity was mediated by NK cells activated by IL-2 or IL-2+anti-CD3 mAb. The cytotoxic activity of T cells was weak but, interestingly, increased in the presence of phytohaemagglutinin A (PHA), assuming that T cells were able to kill HPV-infected keratinocytes when a bridge between T cells and keratinocytes was provided. In conclusion, the organotypic culture of HPV-transformed keratinocytes may provide an effective in vitro model for investigating novel T cell-based immunotherapy protocols for the treatment of HPV-associated lesions. [less ▲]

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See detailThe organotypic culture of HPV-transformed keratinocytes: an effective in vitro model for the development of new immunotherapeutic approaches for mucosal (pre)neoplastic lesions.
Delvenne, Philippe ULg; Hubert, Pascale ULg; Jacobs, Nathalie ULg et al

in Vaccine (2001), 19(17-19), 2557-64

The purpose of this study is to develop a reliable in vitro human model to test new immunotherapeutic approaches for squamous cell carcinoma that develop on mucosal surfaces. The organotypic (raft ... [more ▼]

The purpose of this study is to develop a reliable in vitro human model to test new immunotherapeutic approaches for squamous cell carcinoma that develop on mucosal surfaces. The organotypic (raft) culture permits cells to proliferate and differentiate at an air-liquid interface on a dermal equivalent support. Normal keratinocytes stratify and fully differentiate in a manner similar to the normal squamous epithelial tissues, while human papillomavirus-immortalized and established squamous carcinoma cell lines exhibit dysplastic morphologies similar to (pre)neoplastic lesions seen in vivo. We have demonstrated the ability of these organotypic cultures to be manipulated by altering the epithelial stratification with cytokines (interferon-gamma and tumor necrosis factor-alpha) and by integrating activated lymphocytes or dendritic cells into the in vitro formed epithelial sheet. This model may provide a useful tool to investigate the factors contributing to the presence and function of immunocompetent cells within a neoplastic epithelium that develops on a mucosal surface. [less ▲]

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See detailOrges brassicoles
Monfort, Bruno; Bodson, Bernard ULg

in Livre Blanc: Céréales - Gembloux (2010, February 24)

Aperçu de l'année en orge de brasserie - Résultats d'expérimentations - Recommandations pratiques pour la culture

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See detailOrges brassicoles
Monfort, bruno; Bodson, Bernard ULg

in Livre Blanc: Céréales - Gembloux (2009, February 18)

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See detailL'orgie ou la boucle du temps dans le film noir hollywoodien
Tomasovic, Dick ULg

Conference (2003, March 17)

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See detailL’orgueil de Foucault. Ontologie, expérience et critique
Bolmain, Thomas ULg

Conference (2010, May 20)

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See detailOriani lettore di Francesco Guicciardini
Curreri, Luciano ULg

in Moreno, Paola; Palumbo, Giovanni (Eds.) Francesco Guicciardini : Tra ragione e inquietudine (2005)

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See detailOrientation And Effects Of Mastoparan X On Phospholipid Bicelles
Whiles, Ja.; Brasseur, Robert ULg; Glover, Kj. et al

in Biophysical Journal (2001), 80(1), 280-93

Mastoparan X (MPX: INWKGIAAMAKKLL-NH2) belongs to a family of ionophoric peptides found in wasp venom. Upon binding to the membrane, MPX increases the cell's permeability to cations leading to a ... [more ▼]

Mastoparan X (MPX: INWKGIAAMAKKLL-NH2) belongs to a family of ionophoric peptides found in wasp venom. Upon binding to the membrane, MPX increases the cell's permeability to cations leading to a disruption in the electrolyte balance and cell lysis. This process is thought to occur either through a membrane-thinning mechanism, where the peptide resides on the membrane surface thereby disrupting lipid packing, or through formation of an oligomeric pore. To address this issue, we have used both high-resolution and solid-state 2H NMR techniques to study the structure and orientation of MPX when associated with bicelles. NOESY and chemical shift analysis showed that in bicelles, MPX formed a well-structured amphipathic alpha-helix. In zwitterionic bicelles, the helical axis was found to rest generally perpendicular to the membrane normal, which could be consistent with the "carpet" mechanism for lytic activity. In anionic bicelles, on the other hand, the helical axis was generally parallel to the membrane normal, which is more consistent with the pore model for lytic activity. In addition, MPX caused significant disruption in lipid packing of the negatively charged phospholipids. Taken together, these results show that MPX associates differently with zwitterionic membranes, where it rests parallel to the surface, compared with negatively charged membranes, where it penetrates longitudinally. [less ▲]

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