Regulatory elements controlling pituitary-specific expression of the human prolactin gene

in Molecular & Cellular Biology (1990), 10(9), 4690-700

We have performed transfection and DNase I footprinting experiments to investigate pituitary-specific expression of the human prolactin (hPRL) gene. When fused to the chloramphenicol acetyltransferase ... [more ▼]

We have performed transfection and DNase I footprinting experiments to investigate pituitary-specific expression of the human prolactin (hPRL) gene. When fused to the chloramphenicol acetyltransferase (CAT) reporter gene, 5,000 base pairs of the 5'-flanking sequences of the hPRL gene were able to drive high cat gene expression in prolactin-expressing GH3B6 cells specifically. Deletion analysis indicated that this pituitary-specific expression was controlled by three main positive regulatory regions. The first was located just upstream from the TATA box between coordinates -40 and -250 (proximal region). We have previously shown that three motifs of this region bind the pituitary-specific Pit-1 factor. The second positive region was located in the vicinity of coordinates -1300 to -1750 (distal region). DNase I footprinting assays revealed that eight DNA motifs of this distal region bound protein Pit-1 and that two other motifs were recognized by ubiquitous factors, one of which seems to belong to the AP-1 (jun) family. The third positive region was located further upstream, between -3500 and -5000 (superdistal region). This region appears to enhance transcription only in the presence of the distal region. [less ▲]

A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig

in Nature (2003), 425(6960), 832-836

Most traits and disorders have a multifactorial background indicating that they are controlled by environmental factors as well as an unknown number of quantitative trait loci (QTLs)(1,2). The ... [more ▼]

Most traits and disorders have a multifactorial background indicating that they are controlled by environmental factors as well as an unknown number of quantitative trait loci (QTLs)(1,2). The identification of mutations underlying QTLs is a challenge because each locus explains only a fraction of the phenotypic variation(3,4). A paternally expressed QTL affecting muscle growth, fat deposition and size of the heart in pigs maps to the IGF2 (insulin-like growth factor 2) region(5,6). Here we show that this QTL is caused by a nucleotide substitution in intron 3 of IGF2. The mutation occurs in an evolutionarily conserved CpG island that is hypomethylated in skeletal muscle. The mutation abrogates in vitro interaction with a nuclear factor, probably a repressor, and pigs inheriting the mutation from their sire have a threefold increase in IGF2 messenger RNA expression in postnatal muscle. Our study establishes a causal relationship between a single-base-pair substitution in a non-coding region and a QTL effect. The result supports the long-held view that regulatory mutations are important for controlling phenotypic variation(7). [less ▲]

Regulatory requirements : clinical evaluation of drug therapy

in Reginster, Jean-Yves; Pelletier, J. P.; Martel-Pelletier, J. (Eds.) et al Experimental and Clinical Aspects of Osteoarthritis (1998)

Regulatory sequences in the human prolactin gene

in Labrie, F.; Proulx, L. (Eds.) Endocrinology 1984 (1984)

Regulatory T cells in Wiskott-Aldrich syndrome and after allogeneic transplantation with nonmyeloablative conditioning

Doctoral thesis (2009)

Réguler les enseignements dans une approche qualité l'apport diagnostic du modèle du polygone des paramètres des actions didactiques

in 25ème Congrès de l'Association Internationale de Pédagogie Universitaire (AIPU) - "Le défi de la qualité dans l'enseignement supérieur : vers un changement de paradigme" (2008, May)

Réguler par la science: le cas des aliments fonctionnels

Conference (2013, March 08)

Réhabilitation cognitive en Centre de Jour: une démarche éthique

Conference given outside the academic context (2008)

La réhabilitation des anciens sites industriels : l'expérience wallonne

in Hommes et Terres du Nord (1989), (4), 353-357

Rehabilitation des structures incendiées

Learning material (1991)

Introduction, reconstruction of the fire, steel structures (non destructive testing, effects of heat on steel, behaviour of steel during a fire), reinforced concrete structures (in situ investigation ... [more ▼]

Introduction, reconstruction of the fire, steel structures (non destructive testing, effects of heat on steel, behaviour of steel during a fire), reinforced concrete structures (in situ investigation methods, effects of heat on reinforced concrete), prestressed concrete structures, bibliography (9 references) [less ▲]