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See detailNUCLEAR DELIVERY OF A THERAPEUTIC PEPTIDE BY LONG CIRCULATING pH-SENSITIVE LIPOSOMES: BENEFITS OVER CLASSICAL VESICLES
Ducat, Emilie; Deprez, Juile; Noël, Agnès ULg et al

Poster (2011, December 06)

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See detailNuclear delivery of a therapeutic peptide by long circulating pH-sensitive liposomes: Benefits over classical vesicles.
Ducat, Emilie ULg; Deprez, Julie ULg; Gillet, Aline ULg et al

in International Journal of Pharmaceutics (2011)

The purpose of this study is to propose a suitable vector combining increased circulation lifetime and intracellular delivery capacities for a therapeutic peptide. Long circulating classical liposomes ... [more ▼]

The purpose of this study is to propose a suitable vector combining increased circulation lifetime and intracellular delivery capacities for a therapeutic peptide. Long circulating classical liposomes [SPC:CHOL:PEG-750-DSPE (47:47:6 molar% ratio)] or pH-sensitive stealth liposomes [DOPE:CHEMS:CHOL:PEG(750)-DSPE (43:21:30:6 molar% ratio)] were used to deliver a therapeutic peptide to its nuclear site of action. The benefit of using stealth pH-sensitive liposomes was investigated and formulations were compared to classical liposomes in terms of size, shape, charge, encapsulation efficiency, stability and, most importantly, in terms of cellular uptake. Confocal microscopy and flow cytometry were used to evaluate the intracellular fate of liposomes themselves and of their hydrophilic encapsulated material. Cellular uptake of peptide-loaded liposomes was also investigated in three cell lines: Hs578t human epithelial cells from breast carcinoma, MDA-MB-231 human breast carcinoma cells and WI-26 human diploid lung fibroblast cells. The difference between formulations in terms of peptide delivery from the endosome to the cytoplasm and even to the nucleus was investigated as a function of time. Characterization studies showed that both formulations possess acceptable size, shape and encapsulation efficiency but cellular uptake studies showed the important benefit of the pH-sensitive formulation over the classical one, in spite of liposome PEGylation. Indeed, stealth pH-sensitive liposomes were able to deliver hydrophilic materials strongly to the cytoplasm. Most importantly, when encapsulated in pH-sensitive stealth liposomes, the peptide was able to reach the nucleus of tumorigenic and non tumorigenic breast cancer cells. [less ▲]

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See detailNuclear Energy: Between Legitimacy and Threat
Michel, Quentin ULg

Conference (2013, December 12)

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See detailNuclear Export Control in the European Union
Michel, Quentin ULg

Learning material (2010)

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See detailNuclear export of histone deacetylase 7 during thymic selection is required for immune self-tolerance.
Kasler, Herbert G.; Lim, Hyung W.; Mottet, Denis ULg et al

in EMBO Journal (2012)

Histone deacetylase 7 (HDAC7) is a T-cell receptor (TCR) signal-dependent regulator of differentiation that is highly expressed in CD4/CD8 double-positive (DP) thymocytes. Here, we examine the effect of ... [more ▼]

Histone deacetylase 7 (HDAC7) is a T-cell receptor (TCR) signal-dependent regulator of differentiation that is highly expressed in CD4/CD8 double-positive (DP) thymocytes. Here, we examine the effect of blocking TCR-dependent nuclear export of HDAC7 during thymic selection, through expression of a signal-resistant mutant of HDAC7 (HDAC7-DeltaP) in thymocytes. We find that HDAC7-DeltaP transgenic thymocytes exhibit a profound block in negative thymic selection, but can still undergo positive selection, resulting in the escape of autoreactive T cells into the periphery. Gene expression profiling reveals a comprehensive suppression of the negative selection-associated gene expression programme in DP thymocytes, associated with a defect in the activation of MAP kinase pathways by TCR signals. The consequence of this block in vivo is a lethal autoimmune syndrome involving the exocrine pancreas and other abdominal organs. These experiments establish a novel molecular model of autoimmunity and cast new light on the relationship between thymic selection and immune self-tolerance. [less ▲]

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See detailNuclear Exports Controls in Europe - chapitre consacré à la Belgique
Michel, Quentin ULg

in Müller, Harald (Ed.) Nuclear Exports Controls in Europe (1995)

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See detailNuclear Factor - Kappab-Dependent Regulation of P53 Gene Expression Induced by Daunomycin Genotoxic Drug
Hellin, Anne-Cécile; Calmant, Philippe ULg; Bours, Vincent ULg et al

in Oncogene (1998), 16(9), 1187-95

Anthracycline drugs are widely used for the treatment of solid tumors and leukemia, but the molecular basis of their biological effect is still poorly understood. In the HCT116 colon carcinoma cell line ... [more ▼]

Anthracycline drugs are widely used for the treatment of solid tumors and leukemia, but the molecular basis of their biological effect is still poorly understood. In the HCT116 colon carcinoma cell line, which retains a wild-type inducible p53 gene, we show that the anthracycline daunomycin is a potent inducer of p53 and NF-kappaB transcription factors. Nuclear accumulation of p53 protein occurred because of increased protein stability and enhanced gene expression. In addition, daunomycin induced the p53 promoter through the binding of p50/p65 NF-kappaB heterodimers to the kappaB site in the p53 promoter. Under our conditions, the free radical scavengers NAC and PDTC were not able to block NF-kappaB activation or p53 induction, indicating that reactive oxygen intermediates were not involved in the cellular response to daunomycin stimulation. Overexpression of a stable unresponsive IkappaBalpha mutant in HCT116 cells resulted in a complete inhibition of the NF-kappaB activation but only a partial impairment of the p53 protein accumulation induced by daunomycin. We conclude that the p53-activating signal generated by daunomycin is partially regulated by NF-kappaB. [less ▲]

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See detailNuclear factor kappaB activity and characterization in lung cells from heaves affected horses
Sandersen, Charlotte ULg; Turlej, R. K.; Fievez, Laurence ULg et al

in Pflügers Archiv : European Journal of Physiology (2000), 440(R217),

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See detailNuclear factor kappaB inhibition in bovine mammary epithelial cells reduces intracellular infection by Staphylococcus aureus.
Boulanger, D.; Bureau, Fabrice ULg; Lekeux, Pierre ULg

in Proceedings : 23rd World Buiatrics Congress (2004)

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See detailNuclear Factor-Kappa B, Cancer, and Apoptosis
Bours, Vincent ULg; Bentires-Alj, M.; Hellin, A. C. et al

in Biochemical Pharmacology (2000), 60(8), 1085-9

The role of nuclear factor (NF)-kappa B in the regulation of apoptosis in normal and cancer cells has been extensively studied in recent years. Constitutive NF-kappa B activity in B lymphocytes as well as ... [more ▼]

The role of nuclear factor (NF)-kappa B in the regulation of apoptosis in normal and cancer cells has been extensively studied in recent years. Constitutive NF-kappa B activity in B lymphocytes as well as in Hodgkin's disease and breast cancer cells protects these cells against apoptosis. It has also been reported that NF-kappa B activation by tumor necrosis factor (TNF)-alpha, chemotherapeutic drugs, or ionizing radiations can protect several cell types against apoptosis, suggesting that NF-kappa B could participate in resistance to cancer treatment. These observations were explained by the regulation of antiapoptotic gene expression by NF-kappa B. However, in our experience, inhibition of NF-kappa B activity in several cancer cell lines has a very variable effect on cell mortality, depending on the cell type, the stimulus, and the level of NF-kappa B inhibition. Moreover, in some experimental systems, NF-kappa B activation is required for the onset of apoptosis. Therefore, it is likely that the NF-kappa B antiapoptotic role in response to chemotherapy is cell type- and signal-dependent and that the level of NF-kappa B inhibition is important. These issues will have to be carefully investigated before considering NF-kappa B as a target for genetic or pharmacological anticancer therapies. [less ▲]

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See detailNuclear forward and inelastic spectroscopy on 125Te and Sb2 125Te3 2
Wille, H.-C.; Hermann, Raphaël ULg; Sergueev, I. et al

in Europhysics Letters [=EPL] (2010)

We report on the observation of nuclear forward and nuclear inelastic scattering of synchrotron radiation by Te-125 and the application of both spectroscopic methods to tellurium compounds by using a high ... [more ▼]

We report on the observation of nuclear forward and nuclear inelastic scattering of synchrotron radiation by Te-125 and the application of both spectroscopic methods to tellurium compounds by using a high-resolution backscattering sapphire monochromator in combination with fast detection electronics. The lifetime of the nuclear resonance and the energy of the transition were determined to be 2.131(12) ns and 35493.12(30) eV, respectively. As applications, the nuclear inelastic spectrum in Sb2Te3 and the nuclear forward scattering by Te metal were measured. These measurements open the field of nuclear resonance spectroscopy on tellurium compounds such as thermoelectric and superconducting materials. [less ▲]

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See detailNuclear forward scattering by the 68.7 keV state of 73Ge in CaGeO3 and GeO2
Simon, RE; Sergueev, I; Persson, J et al

in Europhysics Letters [=EPL] (2013), 104

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See detailNuclear imaging methods for non-invasive drug monitoring.
Bhatnagar, A.; Hustinx, Roland ULg; Alavi, A.

in Advanced Drug Delivery Reviews (2000), 41(1), 41-54

Functional imaging techniques provide complimentary information to that provided by structural studies such as MRI and CT. Functional imaging is based upon known parameters such as physiology, metabolism ... [more ▼]

Functional imaging techniques provide complimentary information to that provided by structural studies such as MRI and CT. Functional imaging is based upon known parameters such as physiology, metabolism, biochemistry, pharmacology, and any other biological process. As such, this methodology plays a major role in understanding the basic mechanisms of a multitude of disorders, accurate diagnosis of certain diseases, and developing effective treatment for serious illnesses such as cancer and central nervous system maladies. Although this type of imaging can be performed with various modalities, nuclear procedures have played the leading role in this discipline. Advances made in labeling various radionuclides to biologically important compounds, and development of sophisticated instruments have substantially contributed to the growth of the field of functional imaging. The introduction of positron emission topography (PET), which is based on imaging of compounds labeled with elements such as carbon, nitrogen, and fluorine, has added a major dimension to the evolution of the discipline. This review deals with a brief introduction to the methodologies utilized with radiolabeled tracers and then deals with specific applications of this technology. These applications include assessment of blood flow and metabolism, receptor imaging, elucidating the pathophysiologic process, evaluating role of labeled therapeutic agents, and the potential of these techniques in the development of novel biologic therapies. Functional imaging with radiolabeled tracers will play an increasingly important role in modern medicine, and its impact will be substantial in the management of patients with various disorders. [less ▲]

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See detailNuclear Lesions During Rat Hepatocarcinogenesis. I. Measuring the Sister-Chromatid Exchanges During Initiation, Promotion and Progression of Rat Hepatocarcinogenesis Induced with Diethylnitrosamine
Herens, Christian ULg; Jacquemart, M.; Koulischer, Lucien ULg et al

in Mutation Research : Fundamental & Molecular Mechanisms of Mutagenesis (1995), 329(2), 153-9

Cytogenetic endpoints such as sister-chromatid exchanges (SCEs), chromosomal aberrations and micronuclei (MNs) have been widely used as indicators of genetic damage. However, no systematic attempts have ... [more ▼]

Cytogenetic endpoints such as sister-chromatid exchanges (SCEs), chromosomal aberrations and micronuclei (MNs) have been widely used as indicators of genetic damage. However, no systematic attempts have been made to correlate the levels of these cytogenetic endpoints with the different steps of carcinogenesis. In the present report, the induction, accumulation and persistence of SCEs and high frequency cells (HFCs) were measured in liver cells during the initiation, promotion and progression steps of rat hepatocarcinogenesis induced by diethylnitrosamine (DEN). The results indicate that lesions leading to SCEs accumulate during initiation only. When DEN administration is longer than the duration of this first step, SCE values stabilize. After stopping the carcinogenic treatment, the SCE levels decrease to control values whether or not promotion and progression occur. [less ▲]

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See detailNuclear Lesions During Rat Hepatocarcinogenesis. II. Measuring the Micronuclei During Initiation, Promotion and Progression of Rat Hepatocarcinogenesis Induced with Diethylnitrosamine
Herens, Christian ULg; Massart, Sandrina ULg; Bouzahzah, B. et al

in Mutation Research : Fundamental & Molecular Mechanisms of Mutagenesis (1995), 329(2), 161-71

We reported in our companion paper the strong correlation between elevated sister-chromatid exchange (SCE) frequencies and the initiation step of rat hepatocarcinogenesis. We have also shown that SCEs ... [more ▼]

We reported in our companion paper the strong correlation between elevated sister-chromatid exchange (SCE) frequencies and the initiation step of rat hepatocarcinogenesis. We have also shown that SCEs return to normal values during the promotion and the progression stages. In the present study, we evaluated the clastogenic activity of diethylnitrosamine (DEN) during initiation, promotion and progression of rat hepatocarcinogenesis. We measured, at various times after DEN administration, the number of micronuclei (MN) produced by the mitotic response to partial hepatectomy. The results established that the DEN treatment induces a great number of preclastogenic lesions. In subcarcinogenic conditions (initiation alone), the number of MN expressed after partial hepatectomy remains high regardless of the time interval between the end of the DEN treatment and the operation. In this condition, the preclastogenic lesions persist for up to 1 year after the DEN administration is discontinued. Conversely, in carcinogenic conditions (initiation + promotion + progression), the number of MN expressed after partial hepatectomy decreases during the promotion and progression stages. These observations indicate that promotion and progression but not initiation are associated with the expression of persistent preclastogenic lesions, resulting in the production of chromosomally abnormal hepatocytes. [less ▲]

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See detailNuclear Localization of a New C-Cbl Related Protein, Carp 90, During in Vivo Thymic Apoptosis in Mice
Denis, Ghislaine; Mandard, S.; Verlaet, Myriam ULg et al

in Cell Death & Differentiation (1999), 6(7), 689-97

This study investigates the involvement of the c-cbl protooncogene in thymocyte apoptosis occurring in vivo after hydrocortisone treatment. In the thymus of untreated mice, a few medullary and cortical ... [more ▼]

This study investigates the involvement of the c-cbl protooncogene in thymocyte apoptosis occurring in vivo after hydrocortisone treatment. In the thymus of untreated mice, a few medullary and cortical thymocytes expressed p120cbl, mainly in the cytoplasm. In the cortex, their number and distribution resemble that of apoptotic cells evidenced by TUNEL staining. The expression of Cbl is rapidly increased when apoptosis is triggered by hydrocortisone. This Cbl-specific immunostaining was detected in the nucleus and is due to a Cbl-related 90 kDa protein (CARP 90). These results show that a c-cbl product could localize in the nucleus and suggest that it could be involved as a regulator of thymic apoptosis. [less ▲]

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See detailNuclear magnetic relaxation studies on actinide ions and models of actinide complexes
Desreux, Jean-François ULg

in Advances in Inorganic Chemistry - Including Bioinorganic Studies, Vol 57 (2005)

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See detailNuclear Magnetic Resonance and Nuclear Waste Reprocessing.
Vidick, Geoffrey ULg; Bouslimani, Nouri; Desreux, Jean-François ULg

Poster (2011, November 22)

Detailed reference viewed: 21 (3 ULg)